Advanced search
Publication Cover
Platelets Volume 31, 2020 - Issue 7
Submit an article Journal homepage
798
Views
15
CrossRef citations to date
0
Altmetric
Methods Article

Guidelines for panel design, optimization, and performance of whole blood multi-color flow cytometry of platelet surface markers

Xavier Busuttil-Crellin1 Haematology Research Laboratory, Murdoch Children’s Research Institute, Melbourne, Australia;2 Department of Paediatrics, The University of Melbourne, Melbourne, Australia
,
Conor McCafferty1 Haematology Research Laboratory, Murdoch Children’s Research Institute, Melbourne, Australia
,
Suelyn Van Den Helm1 Haematology Research Laboratory, Murdoch Children’s Research Institute, Melbourne, Australia
,
Hui Ping Yaw1 Haematology Research Laboratory, Murdoch Children’s Research Institute, Melbourne, Australia;2 Department of Paediatrics, The University of Melbourne, Melbourne, Australia
,
Paul Monagle1 Haematology Research Laboratory, Murdoch Children’s Research Institute, Melbourne, Australia;2 Department of Paediatrics, The University of Melbourne, Melbourne, Australia;3 Department of Clinical Haematology, Royal Children’s Hospital, Melbourne, Australia
,
Matthew Linden4 School of Biosciences, The University of Western Australia, Perth, Australia
&
Vera Ignjatovic1 Haematology Research Laboratory, Murdoch Children’s Research Institute, Melbourne, Australia;2 Department of Paediatrics, The University of Melbourne, Melbourne, AustraliaCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0002-5548-5813
ORCID Icon show all
Pages 845-852 | Received 24 Sep 2019, Accepted 20 Dec 2019, Published online: 06 Jan 2020

References

  • Linden MD, Frelinger AL 3rd, Barnard MR, Przyklenk K, Furman MI, Michelson AD. Application of flow cytometry to platelet disorders. Semin Thromb Hemost 2004;30:501–511.
  • Gresele P. Diagnosis of inherited platelet function disorders: guidance from the SSC of the ISTH. J Thromb Haemost 2015;13:314–322. doi:10.1111/jth.2015.13.issue-2.
  • Mezzano D, Quiroga T, Pereira J. The level of laboratory testing required for diagnosis or exclusion of a platelet function disorder using platelet aggregation and secretion assays. Semin Thromb Hemost 2009;35:242–254. doi:10.1055/s-0029-1220785.
  • Michelson A. Flow cytometry: a clinical test of platelet function. Blood 1996;87:4925–4936. doi:10.1182/blood.V87.12.4925.bloodjournal87124925.
  • Tan CW, Bourcy M, Pasalic L, Chen VM. Flow cytometry assessment of procoagulant platelets using a dithiol-reactive probe. Methods Mol Biol 2019;1967:305–321.
  • Mallouk N, Varvat J, Berger A, Epinat M, Accassat S, Garcin A, Montmartin A, Li G, Garnier P, Mismetti P, et al. Assessment of a flow cytometry technique for studying signaling pathways in platelets: monitoring of VASP phosphorylation in clinical samples. Pract Lab Med 2018;11:10–18. doi:10.1016/j.plabm.2018.02.002.
  • Assinger A, Volf I, Schmid D, Obukhov AG. A novel, rapid method to quantify intraplatelet calcium dynamics by ratiometric flow cytometry. PLoS One 2015;10:e0122527. doi:10.1371/journal.pone.0122527.
  • Hui H, Fuller K, Erber WN, Linden MD. Measurement of monocyte-platelet aggregates by imaging flow cytometry. Cytometry A 2015;87:273–278.
  • Pasalic L, Pennings GJ, Connor D, Campbell H, Kritharides L, Chen VM. Flow cytometry protocols for assessment of platelet function in whole blood. Methods Mol Biol 2017;1646:369–389.
  • Ashhurst TM, Smith AL, King NJC. High-dimensional fluorescence cytometry. Curr Protoc Immunol 2017;119:5.8.1–5.8.38. doi:10.1002/cpim.37.
  • Linden MD. Platelet Flow Cytometry. In: editor, Monagle P. Haemostasis: methods and Protocols. New York, NJ, USA: Humana Press; 2013. 241–262. doi:10.1007/978-1-62703-339-8_18.
  • Linden MD. Platelet Physiology. In: editor, Monagle P. Haemostasis: methods and Protocols. New York, NJ, USA: Humana Press; 2013. 13–30. doi:10.1007/978-1-62703-339-8_2.
  • Kazemi A, Abolghasemi H, Kazemzadeh S, Vahidi R, Faranoush M, Farsinejad A, Ala F. Molecular characterization of Glanzmann’s thrombasthenia in Iran: identification of three novel mutations. Blood Coagul Fibrin 2017;681. doi:10.1097/MBC.0000000000000673.
  • FluoroFinder – Fluorescent Experiment Design. Available from: https://fluorofinder.com/
  • Mahnke YD, Roederer M. Optimizing a multicolor immunophenotyping assay. Clin Lab Med 2007;27:469–485. doi:10.1016/j.cll.2007.05.002.
  • Keeney M, Gratama JW, Chin-Yee IH, Sutherland DR. Isotype controls in the analysis of lymphocytes and CD34+ stem and progenitor cells by flow cytometry?time to let go! Cytometry 1998;34:280–283. doi:10.1002/(ISSN)1097-0320.
  • Cossarizza A, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies. Eur J Immunol 2017;47:1584–1797.
  • Maecker HT, Trotter J. Flow cytometry controls, instrument setup, and the determination of positivity. Cytometry Part A 2006;69A:1037–1042. doi:10.1002/(ISSN)1552-4930.
  • Daly ME. Determinants of platelet count in humans. Haematologica 2011;96:10–13. doi:10.3324/haematol.2010.035287.
  • Hulspas R, O’Gorman MRG, Wood BL, Gratama JW, Sutherland DR. Considerations for the control of background fluorescence in clinical flow cytometry. Cytometry Part B 2009;76B:355–364. doi:10.1002/cyto.b.20485.
  • Maecker HT, Frey T, Nomura LE, Trotter J. Selecting fluorochrome conjugates for maximum sensitivity. Cytometry Part A 2004;62A:169–173. doi:10.1002/(ISSN)1097-0320.
  • Yip C, Linden MD, Attard C, Monagle P, Ignjatovic V. Platelets from children are hyper-responsive to activation by thrombin receptor activator peptide and adenosine diphosphate compared to platelets from adults. Br J Haematol 2015;168:526–532. doi:10.1111/bjh.2015.168.issue-4.
  • Guthikonda S, Mangalpally K, Vaduganathan M, Patel R, DeLao T, Bergeron AL, Dong J-F, Lev EI, Kleiman NS, et al. Increased platelet sensitivity among individuals with aspirin resistance - platelet aggregation to submaximal concentration of arachidonic acid predicts response to antiplatelet therapy. Thromb Haemost 2008;100:83–89. doi:10.1160/TH07-10-0590.
  • Fluidics of a Flow Cytometer - AU. Available from: https://www.thermofisher.com/au/en/home/life-science/cell-analysis/cell-analysis-learning-center/molecular-probes-school-of-fluorescence/flow-cytometry-basics/flow-cytometry-fundamentals/fluidics-flow-cytometer.html

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.