1,368
Views
1
CrossRef citations to date
0
Altmetric
Methods and Methods Development

Variation in activation marker expression within the platelet population – a new parameter for evaluation of platelet flow cytometry data

, , &
Pages 1113-1118 | Received 16 Dec 2021, Accepted 10 May 2022, Published online: 18 Jul 2022

References

  • Ramstrom S, Sodergren AL, Tynngard N, Lindahl TL. Platelet function determined by flow cytometry: new perspectives? Semin Thromb Hemost 2016;42(3):268–281. Epub 2016/02/18. doi:10.1055/s-0035-1570082.
  • Boknas N, Macwan AS, Sodergren AL, Ramstrom S. Platelet function testing at low platelet counts: when can you trust your analysis? Res Pract Thromb Haemost 2019;3(2):285–290. Epub 2019/04/24. doi:10.1002/rth2.12193.
  • Shattil SJ, Cunningham M, Hoxie JA. Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry. Blood 1987;70(1):307–315. Epub 1987/07/01. doi:10.1182/blood.V70.1.307.307.
  • Soderstrom AC, Nybo M, Nielsen C, Vinholt PJ. The effect of centrifugation speed and time on pre-analytical platelet activation. Clin Chem Lab Med 2016;54(12):1913–1920. Epub 2016/11/02. doi:10.1515/cclm-2016-0079.
  • Merolla M, Nardi MA, Berger JS. Centrifugation speed affects light transmission aggregometry. Int J Lab Hematol 2012;34(1):81–85. Epub 2011/07/29. doi:10.1111/j.1751-553X.2011.01360.x.
  • Michelson AD. Flow cytometry: a clinical test of platelet function. Blood 1996;87(12):4925–4936. doi:10.1182/blood.V87.12.4925.bloodjournal87124925.
  • Burgstaler EA. Blood component collection by apheresis. J Clin Apher 2006;21(2):142–151. Epub 2005/05/10. doi:10.1002/jca.20043.
  • Lagerberg JW, Salado-Jimena JA, Lof H, Bontekoe IJ, Nielsen C, Verheggen C, van Waeg G, van der Meer PF, de Korte D, Hansen MB, et al. Evaluation of the quality of blood components obtained after automated separation of whole blood by a new multiunit processor. Transfusion 2013;53(8):1798–1807. Epub 2012/12/12. doi:10.1111/trf.12010.
  • Lindahl TL, Festin R, Larsson A. Studies of fibrinogen binding to platelets by flow cytometry: an improved method for studies of platelet activation. Thromb Haemost 1992;68(2):221–225. doi:10.1055/s-0038-1656352.
  • Hulspas R, O’Gorman MR, Wood BL, Gratama JW, Sutherland DR. Considerations for the control of background fluorescence in clinical flow cytometry. Cytometry B Clin Cytom 2009;76(6):355–364. Epub 2009/07/04. doi:10.1002/cyto.b.20485.
  • Sodergren AL, Ramstrom S. Platelet subpopulations remain despite strong dual agonist stimulation and can be characterised using a novel six-colour flow cytometry protocol. Sci Rep 2018;8(1):1441. Epub 2018/01/25. doi:10.1038/s41598-017-19126-8.
  • Beckman coulter technical documents: kaluza analysis software instructions for use. [Accessed 2021-07-27]. Available from https://www.beckman.com/search#q=kaluza&t=coveo-tab-techdocs
  • Ramstrom S, O’Neill S, Dunne E, Kenny D. Annexin V binding to platelets is agonist, time and temperature dependent. Platelets 2010;21(4):289–296. Epub 2010/03/17. doi:10.3109/09537101003660564.
  • Dachary-Prigent J, Freyssinet JM, Pasquet JM, Carron JC, Nurden AT. Annexin V as a probe of aminophospholipid exposure and platelet membrane vesiculation: a flow cytometry study showing a role for free sulfhydryl groups. Blood 1993;81(10):2554–2565. doi:10.1182/blood.V81.10.2554.2554.
  • International clinical cytometry society: what is MFI and how is it calculated?. [Accessed 2021-07-27]. Available from https://www.cytometry.org/web/q_view.php?id=152&filter=Analysis%20Techniques
  • Schmitz G, Rothe G, Ruf A, Barlage S, Tschope D, Clemetson KJ, Goodall AH, Michelson AD, Nurden AT, Shankey TV. European working group on clinical cell analysis: consensus protocol for the flow cytometric characterisation of platelet function. Thromb Haemost 1998;79(5):885–896. Epub 1998/06/03. doi:10.1055/s-0037-1615088.
  • Hui KY, Jakubowski JA, Wyss VL, Angleton EL. Minimal sequence requirement of thrombin receptor agonist peptide. Biochem Biophys Res Commun 1992;184(2):790–796. doi:10.1016/0006-291X(92)90659-9.
  • Hollenberg MD, Saifeddine M, Sandhu S, Houle S, Vergnolle N. Proteinase-activated receptor-4: evaluation of tethered ligand-derived peptides as probes for receptor function and as inflammatory agonists in vivo. Br J Pharmacol 2004;143(4):443–454. doi:10.1038/sj.bjp.0705946.
  • Morton LF, Hargreaves PG, Farndale RW, Young RD, Barnes MJ. Integrin alpha 2 beta 1-independent activation of platelets by simple collagen-like peptides: collagen tertiary (triple-helical) and quaternary (polymeric) structures are sufficient alone for alpha 2 beta 1-independent platelet reactivity. Biochem J 1995;306:337–344. doi:10.1042/bj3060337.
  • Ranganathan P, Pramesh CS, Buyse M. Common pitfalls in statistical analysis: clinical versus statistical significance. Perspect Clin Res 2015;6(3):169–170. Epub 2015/08/01. doi:10.4103/2229-3485.159943.
  • Anderson AA. Assessing statistical results: magnitude, precision, and model uncertainty. Am Stat 2019;73(sup1):118–121. doi:10.1080/00031305.2018.1537889.
  • Levin E, Serrano K, Devine DV. Biomedical excellence for safer transfusion C. Standardization of CD62P measurement: results of an international comparative study. Vox Sang 2013;105(1):38–46. Epub 2013/02/07. doi:10.1111/vox.12023.