Platelet mass cytometry reveals dysregulation of prothrombotic pathways in essential thrombocythemia

References

• Spivak JL. Myeloproliferative neoplasms. New Engl J Medicine. 2017;376(22):2168–9. doi:10.1056/nejmra1406186.
   PubMed Web of Science ®Google Scholar
• Barbui T. Refining prognostication of thrombosis in ET. Am J Hematol. 2016;91(4):361–3. doi:10.1002/ajh.24320.
   PubMed Web of Science ®Google Scholar
• Barbui T, Barosi G, Birgegard G, Cervantes F, Finazzi G, Griesshammer M, Harrison C, Hasselbalch HC, Hehlmann R, Hoffman R. et al. Philadelphia-negative classical myeloproliferative neoplasms: Critical concepts and management recommendations from European LeukemiaNet. J Clin Oncol. 2011;29(6):761–70. doi:10.1200/jco.2010.31.8436.
   PubMed Web of Science ®Google Scholar
• Tefferi A, Pardanani A, Solomon CG. Essential thrombocythemia. N Engl J Med. 2019;381(22):2135–44. doi:10.1056/nejmcp1816082.
   PubMed Web of Science ®Google Scholar
• Barbui T, Finazzi G, Carobbio A, Thiele J, Passamonti F, Rumi E, Ruggeri M, Rodeghiero F, Randi ML, Bertozzi I. et al. Development and validation of an international prognostic score of thrombosis in World Health Organization–essential thrombocythemia (IPSET-thrombosis). Blood. 2012;120(26):5128–33. doi:10.1182/blood-2012-07-444067.
   PubMed Web of Science ®Google Scholar
• Braunstein EM, Chaturvedi S. Aspirin in ET: will twice a day keep thrombosis away? Blood. 2020;136(2):151–3. doi:10.1182/blood.2020005970.
   PubMed Web of Science ®Google Scholar
• Arellano-Rodrigo E, Alvarez-Larrán A, Reverter JC, Villamor N, Colomer D, Cervantes F. Increased platelet and leukocyte activation as contributing mechanisms for thrombosis in essential thrombocythemia and correlation with the JAK2 mutational status. Haematologica. 2006;91(2):169–75.
   PubMed Web of Science ®Google Scholar
• Barbui T, Falanga A. Molecular biomarkers of thrombosis in myeloproliferative neoplasms. Thromb Res. 2016;140:71–5. doi:10.1016/s0049-3848(16)30102-5.
   PubMed Web of Science ®Google Scholar
• Falanga A, Marchetti M. Thrombosis in myeloproliferative neoplasms. Semin Thromb Hemostasis. 2014;40(3):348–58. doi:10.1055/s-0034-1370794.
   PubMed Web of Science ®Google Scholar
• Oyarzún CPM, Glembotsky AC, Goette NP, Lev PR, Luca GD, Pietto MCB, Moiraghi B, Castro Ríos MA, Vicente A, Marta RF. et al. Platelet toll-like receptors mediate thromboinflammatory responses in patients with essential thrombocythemia. Front Immunol. 2020;11:705. doi:10.3389/fimmu.2020.00705.
   PubMed Web of Science ®Google Scholar
• Karakantza M, Giannakoulas NC, Zikos P, Sakellaropoulos G, Kouraklis A, Aktypi A, Metallinos IC, Theodori E, Zoumbos NC, Maniatis A. et al. Markers of endothelial and in vivo platelet activation in patients with essential thrombocythemia and polycythemia vera. Int J Hematol. 2004;79(3):253–9. doi:10.1532/ijh97.e0316.
   PubMed Web of Science ®Google Scholar
• Falanga A, Marchetti M, Vignoli A, Balducci D, Barbui T. Leukocyte-platelet interaction in patients with essential thrombocythemia and polycythemia vera. Exp Hematol. 2005;33(5):523–30. doi:10.1016/j.exphem.2005.01.015.
   PubMed Web of Science ®Google Scholar
• Hauschner H, Horev MB, Misgav M, Nagar M, Seligsohn U, Rosenberg N, Koren‐Michowitz M. Platelets from Calreticulin mutated essential thrombocythemia patients are less reactive than JAK2 V617F mutated platelets. Am J Hematol. 2020;95(4):379–86. doi:10.1002/ajh.25713.
   PubMed Web of Science ®Google Scholar
• Bongiovanni D, Klug M, Lazareva O, Weidlich S, Biasi M, Ursu S, Warth S, Buske C, Lukas M, Spinner CD. et al. SARS-CoV-2 infection is associated with a pro-thrombotic platelet phenotype. Cell Death Disease. 2021;12(1):50. doi:10.1038/s41419-020-03333-9.
   PubMed Web of Science ®Google Scholar
• Klug M, Kirmes K, Han J, Lazareva O, Rosenbaum M, Viggiani G, von Scheidt M, Ruland J, Baumbach J, Condorelli G, Laugwitz K-L. et al. Mass cytometry of platelet-rich plasma: a new approach to analyze platelet surface expression and reactivity. Platelets. 2022;33(6):1–8. Published online 2021. doi:10.1080/09537104.2021.2009453.
   PubMed Web of Science ®Google Scholar
• Arber DA, Orazi A, Hasserjian R, Thiele J, Borowitz MJ, Beau MML, Bloomfield CD, Cazzola M, Vardiman JW. The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia. Blood. 2016;127(20):2391–405. doi:10.1182/blood-2016-03-643544.
   PubMed Web of Science ®Google Scholar
• Klug M, Lazareva O, Kirmes K, Rosenbaum M, Lukas M, Weidlich S, Spinner CD, von Scheidt M, Gosetti R, Baumbach J. et al. Platelet surface protein expression and reactivity upon TRAP stimulation after BNT162b2 vaccination. Thromb Haemostasis. 2022;122(10):1706–11. Published online 2021. doi:10.1055/s-0041-1733934.
   PubMed Web of Science ®Google Scholar
• Sumatoh HR, Teng KWW, Cheng Y, Newell EW. Optimization of mass cytometry sample cryopreservation after staining. Cytom Part A. 2017;91(1):48–61. doi:10.1002/cyto.a.23014.
   PubMedGoogle Scholar
• Kotecha N, Krutzik PO, Irish JM. Web‐based analysis and publication of flow cytometry experiments. Curr Protoc Cytom. 2010;53(1):10.17.1–10.17.24. doi:10.1002/0471142956.cy1017s53.
   Google Scholar
• Arend L, Bernett J, Manz Q, Klug M, Lazareva O, Baumbach J. et al. A systematic comparison of novel and existing differential analysis methods for CyTOF data. Brief Bioinform. 2022;23(1):bbab471. Published online 2021. doi:10.1093/bib/bbab471.
   PubMed Web of Science ®Google Scholar
• Belkina AC, Ciccolella CO, Anno R, Halpert R, Spidlen J, Snyder-Cappione JE. Automated optimized parameters for T-distributed stochastic neighbor embedding improve visualization and analysis of large datasets. Nat Commun. 2019;10(1):5415. doi:10.1038/s41467-019-13055-y.
   PubMed Web of Science ®Google Scholar
• Gassen SV, Callebaut B, Helden MJV, Lambrecht BN, Demeester P, Dhaene T, Saeys Y. FlowSOM: Using self‐organizing maps for visualization and interpretation of cytometry data. Cytom Part A. 2015;87(7):636–45. doi:10.1002/cyto.a.22625.
   PubMedGoogle Scholar
• Zeiler M, Moser M, Mann M. Copy number analysis of the murine platelet proteome spanning the complete abundance range. Molecular & Cellular Proteomics: MCP. 2014;13(12):3435–45. doi:10.1074/mcp.m114.038513.
   PubMed Web of Science ®Google Scholar
• Nieswandt B, Watson SP. Platelet-collagen interaction: is GPVI the central receptor? Blood. 2003;102(2):449–61. doi:10.1182/blood-2002-12-3882.
   PubMed Web of Science ®Google Scholar
• Kim SJ, Jenne CN. Role of platelets in neutrophil extracellular trap (NET) production and tissue injury. Semin Immunol. 2016;28(6):546–54. doi:10.1016/j.smim.2016.10.013.
   PubMed Web of Science ®Google Scholar
• Sehayek E, Ben-Yosef N, Modan M, Chetrit A, Meytes D. Platelet parameters and aggregation in essential and reactive thrombocytosis. Am J Clin Pathol. 1988;90(4):431–6. doi:10.1093/ajcp/90.4.431.
   PubMed Web of Science ®Google Scholar
• Lee E, Kim M, Jeon K, Lee J, Lee JS, Kim HS, Kang, HJ, Lee, YK. Mean platelet volume and platelet distribution width indicate that platelets remain small for most of their lifespans in patients with essential thrombocythemia. Clin Lab. 2019;65(4/2019). doi:10.7754/clin.lab.2018.180938.
   Web of Science ®Google Scholar
• Lador A, Leshem-Lev D, Spectre G, Abelow A, Kornowski R, Lev EI. Characterization of surface antigens of reticulated immature platelets. J Thromb Thrombolys. 2017;44(3):291–7. doi:10.1007/s11239-017-1533-x.
   PubMed Web of Science ®Google Scholar
• Borst O, Gawaz M. Glycoprotein VI -Novel target in antiplatelet medication. Pharmacol Therapeut. 2020;217:107630. doi:10.1016/j.pharmthera.2020.107630.
   PubMed Web of Science ®Google Scholar
• Schüpke S, Hein-Rothweiler R, Mayer K, Janisch M, Sibbing D, Ndrepepa G, Hilz R, Laugwitz K-L, Bernlochner I, Gschwendtner S. et al. Revacept, a novel inhibitor of platelet adhesion, in patients undergoing elective PCI—design and rationale of the randomized ISAR-PLASTER trial. Thromb Haemostasis. 2019;119(9):1539–45. doi:10.1055/s-0039-1692423.
   PubMed Web of Science ®Google Scholar
• Pedersen OH, Larsen ML, Grove EL, van Kooten Niekerk PB, Bønløkke S, Nissen PH, Kristensen SD, Hvas A-M. Platelet characteristics in patients with essential thrombocytosis. Cytom Part B: Clin Cytom. 2018;94(6):918–27. doi:10.1002/cyto.b.21642.
   PubMed Web of Science ®Google Scholar
• Ryningen A, Apelseth T, Hausken T, Bruserud Ø. Reticulated platelets are increased in chronic myeloproliferative disorders, pure erythrocytosis, reactive thrombocytosis and prior to hematopoietic reconstitution after intensive chemotherapy. Platelets. 2006;17(5):296–302. doi:10.1080/09537100600746508.
   PubMed Web of Science ®Google Scholar
• Veninga A, Handtke S, Aurich K, Tullemans B, Brouns S, Schwarz SL, Heubel-Moenen FCJI, Greinacher A, Heemskerk JWM, van der Meijden PEJ. et al. GPVI expression is linked to platelet size, age, and reactivity. Blood Adv. 2022;6(14):4162–73. doi:10.1182/bloodadvances.2021006904.
   PubMed Web of Science ®Google Scholar