Advanced search
Publication Cover
International Journal of Radiation Biology Volume 78, 2002 - Issue 8
Submit an article Journal homepage
81
Views
24
CrossRef citations to date
0
Altmetric
Research Article

The effect of the ratio of CD4 + to CD8 + T-cells on radiation-induced apoptosis in human lymphocyte subpopulations

R. C. Wilkins Health Canada, Consumer and Clinical Radiation Protection Bureau, 775 Brookfield Road, Postal Locator 6303B, Ottawa, Ontario, K1A 1C1, Canada
,
B. C. Kutzner Health Canada, Consumer and Clinical Radiation Protection Bureau, 775 Brookfield Road, Postal Locator 6303B, Ottawa, Ontario, K1A 1C1, Canada
,
M. Truong Health Canada, Consumer and Clinical Radiation Protection Bureau, 775 Brookfield Road, Postal Locator 6303B, Ottawa, Ontario, K1A 1C1, Canada
&
J. R. N. McLean Health Canada, Consumer and Clinical Radiation Protection Bureau, 775 Brookfield Road, Postal Locator 6303B, Ottawa, Ontario, K1A 1C1, Canada
Pages 681-688 | Published online: 03 Jul 2009

  • AGGARWAL, S., GOLLAPUDI, S., and GUPTA, S., 1999, Increased TNF-a-induced apoptosis in lymphocytes from aged humans: changes in TNF-a receptor expression and activation of caspases. Journal of Immunology, 162, 2154-2161.
  • ANDERSON, R. E., OLSON, G. B., AUTRY, J. R., HOWARTH, J. L., TROUP, G. M., and BARTELS, P. H.,1977a, Radiosensitivity of T and B lymphocytes. IV. Effect of whole body irradiation upon various lymphoid tissues and numbers of recirculating lymphocytes. Journal of Immunology, 118, 1191-1200.
  • ANDERSON, R. E., STANDEFER, J. C., and SCALETTI, J. V., 1977b, Radiosensitivity of defined populations of lymphocytes. VI. Functional, structural, and biochemical consequences of in vitro irradiation. Cell Immunology, 33, 45-61.
  • ASHKENAZI, A. and DIXIT, V. M., 1998, Death receptors: signaling and modulation. Science, 281, 1305-1308.
  • BARBER, G. N., 2000, The interferons and cell death: guardians of the cell or accomplices of apoptosis? Seminars in Cancer Biology, 10, 103-111.
  • BOREHAM, D. R., GALE, K. L., MAVES, S. R., WALKER, J. A., and MORRISON, D. P., 1996, Radiation-induced apoptosis in human lymphocytes: potential as a biological dosimeter. Health Physics, 71, 685-691.
  • COHEN, J. J., 1991, Programmed cell death in the immune system. Advances in Immunology, 50, 55-85.
  • COLLINS, R. J., HARMON, B. V., GOBE, G. C., and KERR, J. F., 1992, Internucleosomal DNA cleavage should not be the sole criterion for identifying apoptosis. International Journal of Radiation Biology, 61, 451-453.
  • CROMPTON, N. E. and OZSAHIN, M., 1997, A versatile and rapid assay of radiosensitivity of peripheral blood leukocytes based on DNA and surface-marker assessment of cytotoxicity. Radiation Research, 147, 55-60.
  • DANCESCU, M., RUBIO-TRUJILLO, M., BIRON, G., BRON, D., DELESPESSE, G., and SARFATI, M., 1992, Interleukin 4 protects chronic lymphocytic leukemic B cells from death by apoptosis and upregulates Bcl-2 expression. Journal of Experimental Medicine, 176, 1319-1326.
  • DURUM, S. K. and GENGOZIAN, N., 1978, The comparative radiosensitivity of T and B lymphocytes. International Journal of Radiation Biology, 34, 1-15.
  • GOLDMAN, M., 2000, [Th2 lymphocytes in man: a new cause of hypereosinophilic syndrome]. Bulletin et Memoires de l'Academie Royale de Medecine de Belgique, 155, 141-145.
  • GOUGEON, M. L., LEDRU, E., NAORA, H., BOCCHINO, M. and LECOEUR, H., 2000, HIV, cytokines and programmed cell death. A subtle interplay. Annals of the New York Academy of Sciences, 926, 30-45.
  • HAGIWARA, E., ABBASI, F., MOR, G., ISHIGATSUBO, Y. and KLINMAN, D. M., 1995, Phenotype and frequency of cells secreting IL-2, IL-4, IL-6, IL-IO, IFN and TNF-alpha in human peripheral blood. Cytokine, 7, 815-822.
  • HASPER, H. J., WEGHORST, R. M., RICHEL, D. J., MEERWALDT, J. H., OLTHUIS, F. M. and SCHENKEVELD, C. E., 2000, A new four-color flow cytometric assay to detect apoptosis in lymphocyte subsets of cultured peripheral blood cells. Cytometry, 40, 167-171.
  • HERTVELDT, K., PHILIPPE, J., THIERENS, H., CORNELISSEN, M., VRAL, A. and DE RIDDER, L., 1997, Flow cytometry as a quantitative and sensitive method to evaluate low dose radiation induced apoptosis in vitro in human peripheral blood lymphocytes. International Journal of Radiation Biology, 71, 429-433.
  • JASON, J. and LARNED, J., 1997, Single-cell cytokine profiles in normal humans: comparison of flow cytometric reagents and stimulation protocols. Journal of Immunological Methods, 207, 13-22.
  • LORENZ, H. M., HIERONYMUS, T., Grunke, M., Manger, B. and KALDEN, J. R., 1997, Differential role for IL-2 and IL-15 in the inhibition of apoptosis in short-term activated human lymphocytes. Scandinavian Journal of Immunology, 45, 660-669.
  • MATSUMURA, R., UMEMIYA, K., GOTO, T., NAKAZAWA, T., OCHIAI, K., KAGAMI, M., TOMIOKA, H., TANABE, E., SUGIYAMA, T. and SUEISHI, M., 2000, Interferon gamma and tumor necrosis factor alpha induce Fas expression and anti-Fas mediated apoptosis in a salivary ductal cell line. Clinical and Experimental Rheumatology, 18, 311-318.
  • MORVAN, P. Y., PICOT, C., DEJOUR, R., GENETET, B., and GENETET, N., 1995, Distinct pattern of IL-2 and IFN-gamma gene expression in CD4 and CD8 T cells: cytofluorometric analysis at a single cell level using non-radioactive probes. Cellular and Molecular Biology, 41, 945-957.
  • NAUJOKAT, C., SEZER, O. and POSSINGER, K., 1999, Tumor necrosis factor-alpha and interferon-gamma induce expression of functional Fas ligand on HT29 and MCF7 adenocarcinoma cells. Biochemical and Biophysical Research Communications, 264, 813-819.
  • NETA, R., 1997, Modulation of radiation damage by cytokines. Stem Cells, 15 Suppl 2, 87-94.
  • NORTH, M. E., IVORY, K., FUNAUCHI, M., WEBSTER, A. D., LANE, A. C. and FARRANT, J., 1996, Intracellular cytokine production by human CD4+ and CD8+ T cells from normal and immunodeficient donors using directly conjugated anti- cytokine antibodies and three-colour flow cytometry. Clinical and Experimental Immunology, 105, 517-522.
  • OZSAHIN, M., OZSAHIN, H., SHI, Y., LARSSON, B., WURGLER, F. E. and CROMPTON, N. E., 1997, Rapid assay of intrinsic radiosensitivity based on apoptosis in human CD4 and CDS T-lymphocytes. International Journal of Radiation Oncology, Biology, Physics, 38, 429-440.
  • PERICLE, F., LIU, J. H., DIAZ, J. I., BLANCHARD, D. K., WEI, S., FORM, G. and DJEU, J. Y., 1994, Interleukin-2 prevention of apoptosis in human neutrophils. European Journal of Immunology, 24, 440-444.
  • PHILIPPE, J., LOUAGIE, H., THIERENS, H., VRAL, A., CORNELISSEN, M. and DE RIDDER, L., 1997, Quantification of apoptosis in lymphocyte subsets and effect of apoptosis on apparent expression of membrane antigens. Cytametry, 29, 242-249.
  • POULY, S., BECHER, B., BLAIN, M. and ANTEL, J. P., 2000, Interferon-gamma modulates human oligodendrocyte susceptibility to Fas- mediated apoptosis. Journal of Neuropathology and Experimental Neurology, 59, 280-286.
  • PROSSER, J. S., 1976, Survival of human T and B lymphocytes after X-irradiation. International Journal of Radiation Biology, 30, 459-465.
  • SEKI, H., IWAI, K., KANEGANE, H., KONNO, A., OHTA, K., YACHIE, A, TANIGUCHI, N. and MIYAWAKI, T., 1995, Differential protective action of cytokines on radiationinduced apoptosis of peripheral lymphocyte subpopulations. Cell Immunology, 163, 30-36.
  • SEMPLE, J. W., ALLEN, D., CHANG, W., CASTALDI, P. and FREEDMAN, J., 1993, Rapid separation of CD4+ and CD19+ lymphocyte populations from human peripheral blood by a magnetic activated cell sorter (MACS). Cytometry, 14, 955-960.
  • STRAUSS, G. H. S., 1991, Non-random cell killing in cryopreservation: Implications for performance of the battery of leukocyte tests (BLT), I. Toxic and immunotoxic effects. Mutation Research, 252, 1-15.
  • VAN RENSBURG, E. J., LOUW, W. K., IZATT, H. and VAN DER WATT, J. J., 1985, DNA supercoiled domains and radiosensitivity of subpopulations of human peripheral blood lymphocytes. International Journal of Radiation Biology, 47, 673-679.
  • VANDENABEELE, P., DECLERCQ, W., VANHAESEBROECK, B., GROOTEN, J. and FIERS, W., 1995, Both TNF receptors are required for TNF-mediated induction of apoptosis in PC60 cells. Journal of Immunology, 154, 2904-2913.
  • VERMES, I., HAANEN, C. and REUTELINGSPERGER, C., 2000, Flow cytometry of apoptotic cell death. Journal of Immunologcial Methods, 243, 167-190.
  • WASSERMAN, J., PETRINI, B. and BLOMGREN, H., 1982, Radiosensitivity of T-lymphocyte subpopulations. Journal of Clinical and Laboratory Immunology, 7, 139-140.
  • WEBER, A. K., WAHN, U. and RENZ, H., 2000, Superantigen-induced T cell death by apoptosis: analysis on a single cell level and effect of IFN-gamma and IL-4 treatment. International Archives of Allergy and Immunology, 121, 215-223.
  • WILKINS, R. C., WILKINSON, D., MAHARAJ, H. P., BELLIER, P. V., CYBULSKI, M. B. and MCLEAN, J. R. N., 2001, Differential apoptotic response to ionizing radiation in subpopulations of human white blood cells. Mutation Research, 513, 27-36.
  • Zaki, M., Douglas, R., Patten, N., Bachinsky, M., Lamb, R., Nowell, P. and MOORE, J., 2000, Disruption of the IFN-gamma cytokine network in chronic lymphocytic leukemia contributes to resistance of leukemic B cells to apoptosis. Leukemia Research, 24, 611-621.
  • ZAMORANO, J., WANG, H. Y., WANG, L. M., PIERCE, J. H. and KEEGAN, A. D., 1996, IL-4 protects cells from apoptosis via the insulin receptor substrate pathway and a second independent signaling pathway. Journal of Immunology, 157, 4926-4934.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.