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Technical Report

A protocol for irradiation of cell lines cultured in multi-well plates: effect of air inhomogeneity on irradiated cell survival

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Pages 1543-1546 | Received 19 Jul 2018, Accepted 03 Jun 2019, Published online: 27 Sep 2019

References

  • Abatzoglou I, Zois CE, Pouliliou S, Koukourakis MI. 2013. Establishment and validation of a method for multi-dose irradiation of cells in 96-well microplates. Biochem Biophys Res Commun. 431:456–459.
  • Attix FH. 2008. Introduction to radiological physics and radiation dosimetry. New York (NY): John Wiley & Sons.
  • Blockhuys S, Vanhoecke B, Paelinck L, Bracke M, De Wagter C. 2009. Development of in vitro models for investigating spatially fractionated irradiation: physics and biological results. Phys Med Biol. 54:1565.
  • Bray MA, Carpenter A. 2013. Advanced assay development guidelines for image-based high content screening and analysis. In: Sittampalam GS, Coussens NP, Brimacombe K, et al., editors. Assay guidance manual. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences.
  • Bromley R, Davey R, Oliver L, Harvie R, Baldock C. 2006. A preliminary investigation of cell growth after irradiation using a modulated x-ray intensity pattern. Phys Med Biol. 51:3639.
  • Buch K, Peters T, Nawroth T, Sänger M, Schmidberger H, Langguth P. 2012. Determination of cell survival after irradiation via clonogenic assay versus multiple MTT assay – a comparative study. Radiat Oncol. 7:1.
  • Claridge Mackonis E, Hammond L, Esteves AI, Suchowerska N. 2017. Radiation dosimetry in cell biology: comparison of calculated and measured absorbed dose for a range of culture vessels and clinical beam qualities. Int J Radiat Biol. 94:1–17.
  • Franken NA, Rodermond HM, Stap J, Haveman J, Van Bree C. 2006. Clonogenic assay of cells in vitro. Nat Protoc. 1:2315–2319.
  • Kulmala J, Rantanen V, Pekkola-Heino K, Tuominen J, Grénman R. 1995. Dosimetry of irradiation models the 96-well clonogenic assay for testing radiosensitivity of cell lines. Acta Oncol. 34:105–109.
  • Ling CC, Li WX, Anderson LL.. 1995. The relative biological effectiveness of I-125 and Pd-103. Int J Radiat Oncol Biol Phys. 32:373–378.
  • Mackonis CE, Suchowerska N, Naseri P, McKenzie DR. 2012. Optimisation of exposure conditions for in vitro radiobiology experiments. Australas Phys Eng Sci Med. 35:151–157.
  • Pelleg J. 2012. Mechanical properties of materials. NewYork (NY): Springer Science & Business Media.
  • Sabater S, Berenguer R, Honrubia-Gomez P, Rivera M, Nuñez A, Jimenez-Jimenez E, Martos A, Ramirez-Castillejo C. 2014. How air influences radiation dose deposition in multiwell culture plates: a Monte Carlo simulation of radiation geometry. J Radiat Res. 55:1009–1014.
  • Sterzing F, Münter MW, Schäfer M, Haering P, Rhein B, Thilmann C, Debus J. 2005. Radiobiological investigation of dose-rate effects in intensity-modulated radiation therapy. Strahlenther Onkol. 181:42–48.
  • Tomic N, Gosselin M, Wan JF, Saragovi U, Podgorsak EB, Evans M, Devic S. 2007. Verification of cell irradiation dose deposition using a radiochromic film. Phys Med Biol. 52:3121.
  • van Tonder A, Joubert AM, Cromarty A. 2015. Limitations of the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared to three commonly used cell enumeration assays. BMC Res Notes. 8:47.

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