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Original Articles

Influence of sample preparation optimization on the accuracy of dose assessment of an automatic non-fluorescent MN scoring system

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Pages 1470-1484 | Received 26 May 2021, Accepted 23 Jul 2021, Published online: 19 Aug 2021

References

  • Ainsbury EA, Lloyd DC. 2010. Dose estimation software for radiation biodosimetry. Health Phys. 98(2):290–295.
  • Castelain P, Van Hummelen P, Deleener A, Kirsch-Volders M. 1993. Automated detection of cytochalasin-B blocked binucleated lymphocytes for scoring micronuclei. Mutagenesis. 8(4):285–293.
  • De Sanctis S, De Amicis A, Di Cristofaro S, Franchini V, Regalbuto E, Mammana G, Lista F. 2014. Cytokinesis-block micronucleus assay by manual and automated scoring: calibration curves and dose prediction. Health Phys. 106(6):745–749.
  • Decordier I, Papine A, Plas G, Roesems S, Vande Loock K, Moreno-Palomo J, Cemeli E, Anderson D, Fucic A, Marcos R, et al. 2008. Automated image analysis of cytokinesis-blocked micronuclei: an adapted protocol and a validated scoring procedure for biomonitoring. Mutagenesis. 24(1):85–93.
  • Decordier I, Papine A, Vande Loock K, Plas G, Soussaline F, Kirsch-Volders M. 2011. Automated image analysis of micronuclei by IMSTAR for biomonitoring. Mutagenesis. 26(1):163–168.
  • Depuydt J, Baeyens A, Barnard S, Beinke C, Benedek A, Beukes P, Buraczewska I, Darroudi F, De Sanctis S, Dominguez I, et al. 2016. RENEB intercomparison exercises analyzing micronuclei (cytokinesis-block micronucleus assay). Int J Radiat Biol. 93(1):36–47.
  • Fenech M. 2000. The in vitro micronucleus technique. Mutat Res Mol Mech Mutagen. 455(1–2):81–95.
  • Fenech M. 2011. Current status, new frontiers and challenges in radiation biodosimetry using cytogenetic, transcriptomic and proteomic technologies. Radiat Meas. 46(9):737–741.
  • Fenech M, Bonassi S. 2011. The effect of age, gender, diet and lifestyle on DNA damage measured using micronucleus frequency in human peripheral blood lymphocytes. Mutagenesis. 26(1):43–49.
  • Fenech M, Chang WP, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E. 2003. HUMN project: detailed description of the scoring criteria for the cytokinesis – block micronucleus assay using isolated human lymphocyte PubMed Commons. Mutat Res. 534(1–2):65–75.
  • Fenech M, Kirsch-Volders M, Rossnerova A, Sram R, Romm H, Bolognesi C, Ramakumar A, Soussaline F, Schunck C, Elhajouji A, et al. 2013. HUMN project initiative and review of validation, quality control and prospects for further development of automated micronucleus assays using image cytometry systems. Int J Hyg Environ Health. 216(5):541–552.
  • Fenech M, Morley AA. 1986. Cytokinesis-block micronucleus method in human lymphocytes: effect of in vivo ageing and low dose X-irradiation. Mutat Res. 161(2):193–198.
  • Frieauff W, Martus HJ, Suter W, Elhajouji A. 2013. Automatic analysis of the micronucleus test in primary human lymphocytes using image analysis. Mutagenesis. 28(1):15–23.
  • Garty G, Chen Y, Salerno A, Turner H, Zhang J, Lyulko O, Bertucci A, Xu Y, Wang H, Simaan N, et al. 2010. The RABIT: a rapid automated biodosimetry tool for radiological triage. Health Phys. 98(2):209–217.
  • Huber R, Braselmann H, Bauchinger M. 1992. Intra- and inter-individual variation of background and radiation-induced micronucleus frequencies in human lymphocytes. Int J Radiat Biol. 61(5):655–661.
  • Hülber T, Kocsis Z, Kis E, Sáfrány G, Pesznyák C. 2020. A scanning and image processing system with integrated design for automated micronucleus scoring. Int J Radiat Biol. 96:5: 628–641.
  • International Atomic Energy Agency (IAEA). 2011. Cytogenetic dosimetry: applications in preparedness for and response to radiation emergencies. Vienna: IAEA. https://www.iaea.org/publications/8735/cytogenetic-dosimetry-applications-in-preparedness-for-and-response-to-radiation-emergencies
  • Kinneberg M, Lyulko MV. 2010. Applications of the cytokinesis-block micronucleus cytome assay on human lymphocytes: construction of a calibration curve for use in the rapid automated biodosimetry tool.
  • Kocsis SZ, Ágoston P, Farkas Gy, Kun-Gazda M, Székely G, Major T, Mihály D, Pesznyák C, Stelzer G, Jorgo K, et al. 2019. HDR, seed-brachyterápia, LINAC és CyberKnife alapú prosztata-sugárkezelés mellékhatásainak összehasonlítása biodozimetriai és klinikai szempontból (Examination of chromosome aberrations and side effects in different prostate cancer radiotherapies). MAGYAR ONKOLÓGIA. 63(1 Suppl): 36–37.
  • Lyulko OV, Garty G, Randers-Pehrson G, Turner HC, Szolc B, Brenner DJ. 2014. Fast image analysis for the micronucleus assay in a fully automated high-throughput biodosimetry system. Radiat Res. 181(2):146–161.
  • Rogan PK, Li Y, Wickramasinghe A, Subasinghe A, Caminsky N, Khan W, Samarabandu J, Wilkins R, Flegal F, Knoll JH. 2014. Automating dicentric chromosome detection from cytogenetic biodosimetry data. Radiat Prot Dosimetry. 159(1–4):95–104.
  • Romm H, Barnard S, Boulay-Greene H, De Amicis A, De Sanctis S, Franco M, Herodin F, Jones A, Kulka U, Lista F, et al. 2013. Laboratory intercomparison of the cytokinesis-block micronucleus assay. Radiat Results. 180(2):120–128.
  • Rossnerova A, Spatova M, Schunck C, Sram RJ. 2011. Automated scoring of lymphocyte micronuclei by the MetaSystems Metafer image cytometry system and its application in studies of human mutagen sensitivity and biodosimetry of genotoxin exposure. Mutagenesis. 26(1):169–175.
  • Seager AL, Shah UK, Brüsehafer K, Wills J, Manshian B, Chapman KE, Thomas AD, Scott AD, Doherty AT, Doak SH, et al. 2014. Recommendations, evaluation and validation of a semi-automated, fluorescent-based scoring protocol for micronucleus testing in human cells. Mutagenesis. 29(3):155–164.
  • Sullivan JM, Prasanna PGS, Grace MB, Wathen LK, Wallace RL, Koerner JF, Coleman CN. 2013. Assessment of biodosimetry methods for a mass-casualty radiological incident: medical response and management considerations. Health Phys. 105(6):540–554.
  • Tamizh Selvan G, Chaudhury NK, Venkatachalam P. 2015. Comparison of results of the manual and automated scoring of micronucleus frequencies in 60Co-irradiated peripheral blood lymphocytes for triage dosimetry. Appl Radiat Isot. 97:70–77.
  • Varga D, Johannes T, Jainta S, Schuster S, Schwarz-Boeger U, Kiechle M, Patino Garcia B, Vogel W. 2004. An automated scoring procedure for the micronucleus test by image analysis. Mutagenesis. 19(5):391–397.
  • Verhaegen F, Vral A, Seuntjens J, Schipper NW, de Ridder L, Thierens H. 1994. Scoring of radiation-induced micronuclei in cytokinesis-blocked human lymphocytes by automated image analysis. Cytometry. 17(2):119–127.
  • Willems P, August L, Slabbert J, Romm H, Oestreicher U, Thierens H, Vral A. 2010. Automated micronucleus (MN) scoring for population triage in case of large scale radiation events. Int J Radiat Biol. 86(1):2–11.

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