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Original Article

The Irradiation of V79 Mammalian Cells by Protons with Energies below 2 MeV. Part II. Measurement of Oxygen Enhancement Ratios and DNA Damage

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Pages 261-277 | Received 27 Nov 1989, Accepted 20 Mar 1990, Published online: 03 Jul 2009

References

  • Barendsen G.W. Responses of cultured cells, tumours and normal tissue to radiations of different linear energy transfer. Current Topics in Radiation Research 1968; 4: 293–356
  • Barendsen G.W., Koot C.J., van der Kersen G.R., Bewley D.K., Field S.B., Parnell C.J. The effect of oxygen on impairment of the proliferative capacity of human cells in culture by ionizing radiations of different LET. International Journal of Radiation Biology 1966; 10: 317–327
  • Belli M., Cherubini R., Finotto S., Moschini G., Sapora O., Simone G., Tabocchini M.A. RBE-LET relationship for the survival of V79 cells irradiated with low energy protons. International Journal of Radiation Biology 1989; 55: 93–104
  • Bradley M.O., Kohn K.W. X-ray induced DNA double-strand break production and repair in mammalian cells as measured by neutral filter elution. Nucleic Acid Research 1979; 7: 793–804
  • Bryant P.E. Enzymatic restriction of mammalian cell DNA using PvuII and BamHI: evidence for the double-strand break origin of chromosomal aberrations. International Journal of Radiation Biology 1984; 46: 57–65
  • Bryant P.E. Enzymatic restriction of mammalian cell DNA: evidence for double-strand breaks as potentially lethal lesions. International Journal of Radiation Biology 1985; 48: 55–60
  • Burns W.G., May R., Baverstock K.F. Oxygen as a product of water radiolysis in high LET tracks. I. The origin of the hydroperoxyl radical in water radiolysis. Radiation Research 1981; 86: 1–19
  • Charlton D.E., Nikjoo H., Humm J.L. Calculation of initial yields of single- and double-strand breaks in cell nuclei from electrons, protons and alpha-particles. International Journal of Radiation Biology 1989; 56: 1–19
  • Coquerelle T.M., Weibezahn K.F., Lücke-Huhle C. Rejoining of double-strand breaks in normal human and ataxia-telangiectasia fibroblasts after exposure to 60Co γ-rays, 241Am α-particles or bleomycin. International Journal of Radiation Biology 1987; 51: 209–218
  • Flick M.B., Warters R.L., Yasui L.S., Kirsch R.E. Measurement of radiation-induced DNA damage using gel-electrophoresis or neutral filter elution shows an increased frequency of DNA strand breaks after exposure to pH 9·6. Radiation Research 1989; 119: 452–465
  • Folkard M., Prise K.M., Vojnovic B., Davies S., Roper M., Michael B.D. The irradiation of V79 mammalian cells by protons with energies below 2 MeV. Part I. Experimental arrangement and measurements of cell survival. International Journal of Radiation Biology 1989; 56: 221–237
  • Fox J.C., McNally N.J. Cell survival and DNA double-strand break repair following X-ray or neutron irradiation of V79 cells. International Journal of Radiation Biology 1988; 54: 1021–1030
  • Frankenberg D., Frankenberg-Schwager M., Blöcher D., Harbich R. Evidence for DNA double-strand breaks as the critical lesions in yeast cells irradiated with sparsely or densely ionizing radiation under oxic or anoxic conditions. Radiation Research 1981; 88: 524–532
  • Goodhead D.T., Nikjoo H. Track structure analysis of ultrasoft X-rays compared to high- and low-LET radiations. International Journal of Radiation Biology 1989; 55: 513–529
  • Iliakis G., Okayasu R. The level of induced DNA double-strand breaks does not correlate with cell killing in X-irradiated mitotic and G1-phase CHO cells. International Journal of Radiation Biology 1988; 53: 395–404
  • Kampf G. Influence of oxygen on the induction of DNA strand breaks in mammalian cells by radiations of different LET and relations to cell killing. I. Experimental results. Studia Biophysica 1982; 88: 87–96
  • Kampf G., Eichhorn K. DNA strand breakage by different radiation qualities and relations to cell killing: further results after the influence of α-particles and carbon ions. Studia Biophysica 1983; 93: 17–26
  • Katz R. RBE, LET and Z*2/β2. Health Physics 1970; 18: 175–175
  • Kellerer A.M., Chmelevsky D. Criteria for the applicability of LET. Radiation Research 1975; 63: 226–234
  • Kraft G. Radiobiological effects of very heavy ions: inactivation, induction of chromosome aberrations and strand breaks. Nuclear Science Applications 1987; 3: 1–28
  • Maki H., Saito M., Kobayashi T., Kawai K., Akaboshi M. Cell inactivation and DNA single- and double-strand breaks in cultured mammalian cells irradiated by a thermal neutron beam. International Journal of radiation Biology 1986; 50: 795–809
  • Moss A.J., Baker M.L., Prior R.M., Erichsen E.A., Nagle W.A., Dalrymple G.V. Fast neutron and X-ray induced single-strand DNA breaks in cultured mammalian cells. Radiology 1976; 119: 459–461
  • Natarajan A.T., Obe G. Molecular mechanisms involved in the production of chromosomal aberrations. I. Utilization of Neurospora endonuclease for the study of aberration production in G2 stage of the cell cycle. Mutation Research 1978; 52: 137–149
  • Natarajan A.T., Obe G., Van Zeeland A.A., Palatti F., Meijers M., Verdegaal-Immerzeel E.A.M. Molecular mechanisms involved in the production of chromosomal aberrations. II. Utilization of Neurospora endonuclease for the study of aberration production by X-rays in G1 and G2 stages of the cell cycle. Mutation Research 1980; 69: 293–305
  • Neary G.L., Simpson-Gildemeister V.F.W., Peacocke A.R. The influence of radiation quality and oxygen on strand breakage in dry DNA. International Journal of Radiation Biology 1970; 18: 25–40
  • Neary G.J., Horgan V.J., Bance D.A., Stretch A. Further data on DNA strand breakage by various radiation qualities. International Journal of Radiation Biology 1972; 22: 525–537
  • Okayasu R., Iliakis G. Linear DNA elution dose-response curves obtained in CHO cells with non-unwinding filter elution after appropriate selection of lysis conditions. International Journal of Radiation Biology 1989; 55: 569–581
  • Painter R.B. The role of DNA damage and repair in cell killing induced by ionizing radiation. Radiation Biology in Cancer Research, R.E. Meyn, H.R. Withers. Raven Press, New York 1980; 59–68
  • Peak J.G., Blazek E.R., Hill C.K., Peak M.J. Measurement of double-strand breaks in Chinese hamster cell DNA by neutral filter elution: calibration by 125I decay. Radiation Research 1988; 115: 624–629
  • Perris A., Pailogou P., Katsanos A.A., Sideris E.G. Biological effectiveness of low energy protons. I. Survival of Chinese hamster cells. International Journal of Radiation Biology 1986; 50: 1093–1101
  • Prise K.M., Davies S., Michael D. The relationship between radiation-induced DNA double-strand breaks and cell kill in hamster V79 fibroblasts irradiated with 250 kVp X-rays, 2·3 MeV neutrons or 238Pu α-particles. International Journal of Radiation Biology 1987; 52: 893–902
  • Prise K.M., Davies S., Michael B.D. An improved method for the treatment of data from DNA strand break measurements using filter elution techniques with an internal standard. International Journal of Radiation Biology 1989a; 55: 323–330
  • Prise K.M., Davies S., Michael B.D. Cell killing and DNA damage in Chinese hamster V79 cells treated with hydrogen peroxide. International Journal of Radiation Biology 1989b; 55: 583–592
  • Prise K.M., Davies S., Michael B.D. Non-linear dose-effect curve for DNA double-strand breaks by low LET radiation: the effect of eluting buffer composition on the measurement of breaks by the filter elution technique. International Journal of Radiation Biology 1989c; 56: 943–950
  • Radford I.R. The level of induced DNA double-strand breakage correlates with cell killing after X-irradiation. International Journal of Radiation Biology 1985; 48: 45–54
  • Radford I.R. Evidence for a general relationship between the induced level of DNA double-strand breakage and cell killing after X-irradiation of mammalian cells. International Journal of Radiation Biology 1986; 49: 611–620
  • Radford I.R. The dose response for low-LET radiation-induced DNA double-strand breakage. Methods for measurement and implications for radiation action models. International Journal of Radiation Biology 1988; 54: 1–11
  • Radford I.R., Hodgson G.S. 125I-induced DNA double-strand breaks: use in calibration of the neutral filter elution technique and comparison with X-ray-induced breaks. International Journal of Radiation Biology 1985; 48: 555–566
  • Van der Schans G.P., Paterson M.C., Cross W.G. DNA strand break and rejoining in cultured human fibroblasts exposed to fast neutrons or gamma-rays. International Journal of Radiation Biology 1983; 44: 75–85
  • Ward J.F., Blakely W.F., Joner E.I. Mammalian cells are not killed by DNA single-strand breaks caused by hydroxyl radicals from hydrogen peroxide. Radiation Research 1985; 103: 383–392
  • Wlodek D., Hittelman W.N. The repair of double-strand DNA breaks correlates with radiosensitivity of L5178Y-S and L5178Y-R cells. Radiation Research 1987; 112: 146–155
  • Zwelling L.A., Michaels S., Erickson L.C., Ungerleider R.S., Nichols M., Kohn K.W. Protein-associated DNA strand breaks in L1210 cells treated with the DNA intercalating agents 4′-(9-acridinylamino) methanesulfon-m-anisidide and adriamycin. Biochemistry 1981; 20: 6553–6563

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