5
Views
13
CrossRef citations to date
0
Altmetric
Original Article

Chromosomal Aberration Induction in CHO Cells by Combined Exposure to Restriction Enzymes and X-rays

, , , , &
Pages 627-634 | Received 15 Nov 1990, Accepted 26 Mar 1991, Published online: 03 Jul 2009

References

  • Ager D.D., Phillips J.W., Abella Columna E., Winegar R.A., Morgan W.F. Analysis of restriction enzyme-induced DNA double-strand breaks in Chinese hamster ovary cells by pulsed-field gel electrophoresis: implications for chromosome damage. Radiation Research 1991, in press
  • Bryant P.E. Enzymatic restriction of mammalian cell DNA using Pvu II and Bam HI: evidence for the double-strand break origin of chromosomal aberrations. International Journal of Radiation Biology 1984; 46: 57–65
  • Bryant P.E. Use of restriction endonucleases to study relationships between DNA double-strand breaks, chromosomal aberrations and other end-points in mammalian cells. International Journal of Radiation Biology 1988; 54: 869–890
  • Bryant P.E. Restriction endonuclease- and radiation-induced DNA double-strand breaks and chromosomal aberrations: Similarities and differences. Chromosomal Aberrations: Basic and Applied Aspects, G. Obe, A.T. Natarajan. Springer-Verlag, Berlin 1990; 61–69
  • Chung H.W., Phillips J.W., Winegar R.A., Preston R.J., Morgan W.F. Modulation of restriction enzyme-induced damage by chemicals that interfere with cellular responses to DNA damage: a cytogenetic and pulsed-field gel analysis. Radiation Research 1991; 125: 107–113
  • Costa N.D., Bryant P.E. The induction of DNA double-strand breaks in CHO cells by Pvu II: kinetics using neutral filter elution (pH 9.6). International Journal of Radiation Biology 1990; 57: 933–938
  • Evans H.J. Chromosome aberrations induced by ionizing radiations. International Review of Cytology 1962; 13: 221–321
  • Evans H.J. Molecular mechanisms in the induction of chromosome aberrations. Progress in Genetic Toxicology, D. Scott, B.A. Bridges, F.H. Sobels. Elsevier/North-Holland Biomedical Press, Amsterdam 1977; 57–74
  • Morgan W.F., Winegar R.A. The use of restriction endonucleases to study the mechanisms of chromosome damage. Chromosomal Aberrations: Basic and Applied Aspects, G. Obe, A.T. Natarajan. Springer-Verlag, Berlin 1990; 70–78
  • Morgan W.F., Ager D., Chung H.W., Ortiz T., Phillips J.W., Winegar R.A. The cytogenetic effects of restriction endonucleases following their introduction into cells by electroporation. Mutation and the Environment, Part B: Metabolism, Testing Methods, and Chromosomes, M.L. Mendelsohn, R.J. Albertini. Wiley-Liss, New York 1990a; 355–361
  • Morgan W.F., Phillips J.W., Chung H.W., Ager D.D., Winegar R.A. The use of restriction endonucleases to mimic the cytogenetic damage induced by ionizing radiations. Ionizing Radiation Damage to DNA: Molecular Aspects, S.S. Wallace, R.B. Painter. Wiley-Liss, New York 1990b; 191–199
  • Natarajan A.T., Obe G. Molecular mechanisms involved in the production of chromosomal aberrations. III. Restriction endonucleases. Chromosoma 1984; 90: 120–127
  • Natarajan A.T., Obe G., Van Zeeland A.A., Palitti F., Meijers M., Verdegaal-Immerzeel E.A.M. Molecular mechanisms involved in the production of chromosomal aberrations. II. Utilization of Neurospora endonuclease for the study of aberration production by X-rays in G1 and G2 stages of the cell cycle. Mutation Research 1980; 69: 293–305
  • Obe G., Winkel E.-U. The chromosome-breaking activity of the restriction endonuclease Alu I in CHO cells is independent of the S-phase of the cell cycle. Mutation Research 1985; 152: 25–29
  • Prempree T., Merz T. Radiosensitivity and repair time: the repair time of chromosome breaks produced during the different stages of the cell cycle. Mutation Research 1969; 7: 441–451
  • Tanzarella C., De Salvia R., Degrassi F., Fiore M., Palitti F. Interaction between X-ray- and restriction endonuclease-induced lesions in the formation of chromosomal aberrations. Mutation Research 1990; 244: 197–200
  • Winegar R.A., Preston R.J. The induction of chromosome aberrations by restriction endonucleases that produce blunt-end or cohesive-end double-strand breaks. Mutation Research 1988; 197: 141–149
  • Winegar R.A., Phillips J.W., Youngblom J.H., Morgan W.F. Cell electroporation is a highly efficient method for introducing restriction endonucleases into cells. Mutation Research 1989; 225: 49–53
  • Winegar R.A., Phillips J.W., Lutze L.H., Morgan W.F. Chromosome aberration induction in Chinese hamster ovary cells by restriction enzymes with different methylation sensitivity. Somatic Cell and Molecular Genetics 1990; 16: 251–256
  • Wolff S. Chromosome aberrations and the cell cycle. Radiation Research 1968; 33: 609–619
  • Wolff S. Chromosomal effects of mutagenic carcinogens and the nature of the lesions leading to sister chromatid exchange. Mutagen-induced Chromosome Damage in Man, H.J. Evans, D.C. Lloyd. Edinburgh University Press, Edinburgh 1978; 208–215
  • Wolff S., Atwood K.C. Independent X-ray effect on chromosome breakage and reunion. Proceedings of the National Academy of Sciences, USA 1954; 40: 187–192

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.