15
Views
35
CrossRef citations to date
0
Altmetric
Original Article

Level of DNA Double-strand Break Rejoining in Chinese Hamster xrs-5 Cells is Dose-dependent: Implications for the Mechanism of Radiosensitivity

, &
Pages 315-321 | Received 22 Apr 1991, Accepted 01 Sep 1991, Published online: 03 Jul 2009

References

  • Ahnstrom G., Bryant P.E. DNA double-strand breaks generated by the repair of x-ray damage in Chinese hamster cells. International Journal of Radiation Biology 1982; 41: 671–676
  • Blöcher D., Pohlet W. DNA double-strand breaks in Ehrlich ascites tumour cells at low doses of X-rays, II. Can cell death be attributed to double-strand breaks?. International Journal of Radiation Biology 1982; 42: 329–338
  • Costa N.D., Bryant P.E. Repair of DNA single-strand and double-strand breaks in the Chinese hamster xrs-5 mutant cell line as determined by DNA-unwinding. Mutation Research 1988; 194: 93–99
  • Darroudi F., Natarajan A.T. Cytological characterization of Chinese hamster ovary X-ray-sensitive mutant xrs5 and xrs6. I. Induction of chromosomal aberrations by X-irradiation and its modulation with 3-aminobenzamide and caffeine. Mutation Research 1987; 177: 133–148
  • Iliakis G., Okayasu R. Radiosensitivity throughout the cell cycle and repair of potentially lethal damage and DNA double-strand breaks in an X-ray-sensitive CHO mutant. International Journal of Radiation Biology 1990; 57: 1195–1211
  • Iliakis G., Pantelias G.E. Production and repair of chromosome damage in an X-ray-sensitive CHO mutant visualized and analyzed in interphase using the technique of premature chromosome condensation. International Journal of Radiation Biology 1990; 57: 1213–1223
  • Iliakis G., Metzger L., Denko N., Stamato T.D. Detection of DNA double strand breaks in synchronous cultures of CHO cells by means of asymmetric field inversion gel electrophoresis. International Journal of Radiation Biology 1991a; 59: 321–341
  • Iliakis G., Okayasu R., Seaner R. Radiosensitive xrs-5 and parental CHO cells show identical DNA neutral filter elution dose-response: implications for a relationship between cell radiosensitivity and induction of DNA double-strand breaks. International Journal of Radiation Biology 1988; 54: 55–62
  • Iliakis G.E., Cicilioni O., Metzger L. Measurement of DNA double strand breaks in CHO cells at various stages of the cell cycle using pulsed field gel electrophoresis: calibrations by means of 125I decay. International Journal of Radiation Biology 1991b; 59: 343–357
  • Iliakis G., Wang Y., Pantelias G, Metzger L. Mechanism of radiosensitization by halogenated pyrimidines: Effect of BrdU on repair of DNA breaks, interphase chromatin breaks and potentially lethal damage in plateau-phase CHO cells. Radiation Research 1991d, (Submitted.)
  • Iliakis G., Bloecher D., Metzger L., Pantelias G. Comparison of DNA double-strand break rejoining as measured by pulsed field gel electrophoresis, neutral sucrose gradient centrifugation and non-unwinding filter elution in irradiated plateau-phase CHO cells. International Journal of Radiation Biology 1991c; 59: 927–939
  • Jeggo P.A. Cenetic analysis of X-ray-sensitive mutants of the CHO cell line. Mutation Research 1985; 146: 265–270
  • Jeggo P.A. Studies on mammalian mutants defective in rejoining double-strand breaks in DNA. Mutation Research 1990; 239: 1–16
  • Jeggo P.A., Holliday R. Azacytidine-induced reactivation of a DNA repair gene in Chinese hamster ovary cells. Molecular Cellular Biology 1986; 6: 2944–2949
  • Jeggo P.A., Kemp L.M. X-Ray-sensitive mutants of Chinese hamster ovary cell line, isolation and cross-sensitivity to other DNA-damage agents. Mutation Research 1983; 112: 313–327
  • Kemp L.M., Jeggo P.A. Radiation-induced chromosome damage in X-ray-sensitive mutants (xrs) of the Chinese hamster ovary cell line. Mutation Research 1986; 166: 255–263
  • Kemp L.M., Sedgwick S.G., Jeggo P.A. X-Ray-sensitive mutants of Chinese hamster ovary cells defective in double-strand break rejoining. Mutation Research 1984; 132: 189–196
  • Metzger L., Iliakis G. Repair kinetics of DNA double strand breaks throughout the cell cycle measured by pulsed field gel electrophoresis. International Journal of Radiation Biology 1991; 59: 1325–1339
  • Weibezahn K.F., Lohrer H., Herrlich P. Double strand break repair and G2 block in Chinese hamster ovary cells and their radiosensitive mutants. Mutation Research 1985; 145: 177–183

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.