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Original Article

DNA Double-strand Breaks Measured by Pulsed-field Gel Electrophoresis in Irradiated Lymphocytes from Normal Humans and Those with Alzheimer's Disease

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Pages 617-622 | Received 26 Oct 1992, Accepted 25 Jan 1993, Published online: 03 Jul 2009

References

  • Ager D.D., Dewey W.C. Calibration of pulsed field gel electrophoresis for measurement of DNA double-strand breaks. International Journal of Radiation Biology 1990; 58: 249–259
  • Ager D.D., Dewey W.C., Gardiner K., Harvey W., Johnson R.T., Waldren C.A. Measurement of radiation-induced DNA double-strand breaks by pulsed-field gel electrophoresis. Radiation Research 1990; 122: 181–187
  • Bender M.A., Griggs H.G., Bedford J.S. Mechanisms of chromosomal aberration production. III: chemicals and ionizing radiation. Mutation Research 1974; 23: 197–212
  • Bender M.A., Rary J.M., Kale R.P. Go chromosomal radiosensitivity in ataxia telangiectasia lymphocytes. Mutation Research 1985; 150: 277–282
  • Blöcher D., Einspenner M., Zalackowski J. CHEF electrophoresis, a sensitive technique-for the determination of DNA double-strand breaks. International Journal of Radiation Biology 1969; 56: 437–448
  • Blöcher D., Kunhi M. DNA double-strand break analysis by CHEF electrophoresis. International Journal of Radiation Biology 1990; 58: 23–34
  • Blöcher D., Pohlit W. DNA double-strand breaks in Ehrlich ascites tumour cells at low doses of X-rays. II. Can cell death be attributed to double-strand breaks?. International Journal of Radiation Biology 1982; 42: 329–338
  • Blöcher D., Sigut D., Hannan M.A. Fibroblasts from ataxia telangiectasia (AT) and AT heterozygotes show an enhanced level of residual DNA double-strand breaks after low dose-rate γ-irradiation as assayed by pulsed field gel electrophoresis. International Journal of Radiation Biology 1991; 60: 791–802
  • Boerrigter M.E.T.I., Vijg J. Single-strand break disappearance in quiescent and phytohaemag-glutinin-stimulated human peripheral blood lymphocytes exposed to a single low dose of γ-radiation. International Journal of Radiation Biology 1992; 61: 95–101
  • Bryant P.E. Enzymatic restriction of mammalian cell DNA using Pvu II and Bam Hl: evidence for the double-strand break origin of chromosomal aberrations. International Journal of Radiation Biology 1984; 46: 57–65
  • Costa N.D., Bryant P.E. Neutral filter elution detects only limited inhibition of double-strand break repair by 9-β-d-arabinofuranosyladenine. Mutation Research 1990; 235: 217–223
  • Iliakis G. The role of DNA double-strand breaks in ionizing radiation-induced killing of eukaryotic cells. BioEssays 1991; 13: 641–648
  • Iliakis G., Blöcher D., Metzger L., Pantelias G. Comparison of DNA double-strand break rejoining as measured by pulsed-field gel electrophoresis, neutral sucrose gradient centrifugation and non-unwinding filter elution in irradiated plateau-phase CHO cells. International Journal of Radiation Biology 1991b; 59: 927–939
  • Iliakis G.E., Metzger L., Denko N., Stamato T.D. Detection of double-strand breaks in synchronous cultures of CHO cells by means of asymmetric field inversion gel electrophoresis. International Journal of Radiation Biology 1991a; 59: 321–341
  • Kemp L.M., Jeggo P.A. Radiation-induced chromosome damage in X-ray-sensitive mutants (xrs) of the Chinese hamster ovary cell line. Mutation Research, DNA Repair Reports 1986; 166: 255–263
  • Mayer P.J., Bradley M.O., Nichols W.W. Incomplete rejoining of DNA double-strand breaks in unstimulated normal human lymphocytes. Mutation Research 1986; 166: 275–285
  • Mayer P.J., Lange C.S., Bradley M.O., Nichols W.W. Age-dependent decline in rejoining of X-ray-induced DNA double-strand breaks in normal human lymphocytes. Mutation Research 1989; 219: 95–100
  • Mayer P.J., Lange C.S., Bradley M.O., Nichols W.W. Gender differences in age-related decline in DNA double-strand break damage and repair in lymphocytes. Annals of Human Biology 1991; 18: 405–415
  • Metzger L., Iliakis G. Kinetics of DNA double-strand break repair throughout the cell cycle as assayed by pulsed-field gel electrophoresis in CHO cells. International Journal of Radiation Biology 1991; 59: 1325–1339
  • Natarajan A.T., Obe G., Van Zeeland A.A., Palitti F., Meijers M., Verdegaal-Immerzell E.A.M. Molecular mechanisms involved in the production of chromosomal aberrations; II. Utilization of Neurospora endonuclease for the study of aberration production by X-rays in G1 and G2 stages of the cell cycle. Mutation Research 1980; 69: 293–305
  • Pantelias G.E., Maillie H.D. Direct analysis of radiation-induced chromosome fragments and rings in unstimulated human peripheral blood lymphocytes by means of the premature chromosome condensation technique. Mutation Research 1985; 149: 67–72
  • Radford I.R. The level of induced DNA double-strand breakage correlates with cell killing after X-irradiation. International Journal of Radiation Biology 1985; 48: 45–54
  • Radford I.R. Effect of cell-cycle position and dose on the kinetics of DNA double-strand breakage repair in X-irradiated Chinese hamster cells. International Journal of Radiation Biology 1987; 52: 555–563
  • Smith T.A.D., Itzhaki R.F. Radiosensitivity of lymphocytes from patients with Alzheimer's disease. Mutation Research 1989; 217: 11–17
  • Smith T.A.D., Itzhaki R.F. Repair of DNA single-strand breaks in lymphocytes from Alzheimer's disease patients. Gerontology 1991; 37: 193–198
  • Smith T.A.D., Neary D., Itzhaki R.F. DNA repair in lymphocytes from young and old individuals and from patients with Alzheimer's disease. Mutation Research 1987; 184: 107–112
  • Tobi S.E., Moquet J.E., Edwards A.A., Lloyd D.C., Itzhaki R.F. Chromosomal radiosensitivity of lymphocytes from Alzheimer's disease patients. Journal of Medicine and Genetics 1990; 27: 437–440

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