Use of the alkaline comet assay to detect DNA repair deficiencies in human fibroblasts exposed to UVC, UVB, UVA and gamma-rays

References

• ANDERSON, D., Yu, T. W., PHILLIPS, B.J., and SCHMEZER, P., 1994, The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay. Mutation Research, 307, 261–271.
   PubMed Web of Science ®Google Scholar
• ARLETT, C. F., HARCOURT, S. A., COLE, J., GREEN, M. H. L., and ANSTEY, A. V., 1992, A comparison of the response of unstimulated and stimulated T-lymphocytes and fibro-blasts from normal, xeroderma pigmentosum and trichothiodystrophy donors to the lethal action of UV-C. Mutation Research, 273, 127–135.
   PubMed Web of Science ®Google Scholar
• ARLETT, C. F., LOWE, J. E., HARCOURT, S. A., WAUGH, A. P. W., COLE, J., ROZA, L., DIFFEY, B. L., MORI, NIKAIDO, 0., and GREEN, M. H. L., 1993, Hypersensitivity of human lymphocytes to UV-B and solar irradiation. Cancer Research, 53, 609–614.
   Google Scholar
• CHURCHILL, M. E., PEAK, J. G., and PEAK, M. J., 1991, Correlation between cell survival and DNA single-strand break repair proficiency in the Chinese hamster ovary cell lines AA8 and EM9 irradiated with 365 nm ultraviolet-A radiation. Photochemistry and Photobiology, 53, 229–236.
   PubMed Web of Science ®Google Scholar
• CLEAVER, J. E., 1968, Defective repair replication of DNA in xeroderma pigmentosum, Nature, 218, 652–656.
   Google Scholar
• CLINGEN, P. H., ARLETT, C. F., COLE, J., WAUGH, A. P. W., LowE, J. E., HARCOURT, S. A., HERMANOVA, N., ROZA, L., MORI, T., NIKAIDO, 0., and GREEN, M. H. L., 1995, Correlation of UVC and UVB cytotoxicity with the induction of specific photoproducts in T-lymphocytes and fibro-blasts from normal human donors. Photochemistry and Photobiology, 61, 163–170.
   Google Scholar
• COLLINS, A. R., AI-GUO, M., and DUTHIE, S. J., 1995, The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells. Mutation Research, 336, 69–77.
   Google Scholar
• CooHILL, T. P., PEAK, M. J., and PEAK, J. G., 1987, The effects of the ultraviolet wavelengths of radiation present in sunlight on human cells in vitro. Photochemistry and Photobiology, 46, 1043–1050.
   PubMedGoogle Scholar
• DAYA-GROSJEANT, L., ROBERT, C., D ROU GARD, C., SUAREZ, H., and SARASIN, A., 1993, High mutation frequency in ras genes of skin tumors isolated from DNA repair deficient xeroderma pigmentosum patients. Cancer Research, 53, 1625–1629.
   PubMedGoogle Scholar
• DROBETSKY, E. A., TURCOTTE, J., and CHATEAUNEUE, A., 1995, A role for ultraviolet A in solar mutagenesis. Proceedings of the National Academy of Sciences, USA, 92, 2350–2354.
   PubMed Web of Science ®Google Scholar
• DUMAZ, N., DROUGARD, C., SARASIN, A., and DAYA-GROSJEAN, L., 1993, Specific UV-induced mutation spectrum in the p53 gene of skin tumors from DNA repair-deficient xeroderma pigmentosum patients. Proceedings of the National Academy of Sciences, USA, 90, 10529–10533.
   PubMed Web of Science ®Google Scholar
• DUMAZ, N., STARY, A., Soussi, T., DAYA-GROSJEAN, L., and SARASIN, A., 1994, Can we predict solar ultraviolet radiation as the causal event in human tumours by analysing the mutation spectra of the p53 gene? Mutation Research, 307, 375–386.
   Google Scholar
• EVENO, E., BOURRE, F., QUILLIET, X., CHEVALLIER-LAGENTE, O., ROZA, L., EKER, A. P. M., KLEIJER, W. J., NIKAIDO, 0., STEFANINI, M., HOEIJMAKERS, J. H. J., BOOTSMA, D., CLEA-VER, J. E., SARASIN, A., and MEZZINA, M., 1995, Different removal of ultraviolet photoproducts in genetically related xeroderma pigmentosum and trichothio-dystrophy diseases. Cancer Research, 55, 4325–4332.
   Google Scholar
• FAIRBAIRN, D. W., OLIVE, P. L., and O'NEILL, K. L., 1995, The comet assay: a comprehensive review. Mutation Research, 339, 37–59.
   Google Scholar
• FREDERICK, J. E., 1993, Ultraviolet sunlight reaching the earth surface: a review of recent research. Photochemistry and Photobiology, 57, 175–178.
   Web of Science ®Google Scholar
• FREEMAN, S. E., HACHAM, H., GANGE, R. W., MAYTUM, D. J., SUTHERLAND, J. C., and SUTHERLAND, B. M., 1989, Wave-length dependence of pyrimidine dimer formation in DNA of human skin irradiated in situ with ultraviolet light. Proceedings of the National Academy of Sciences, USA, 86, 5605–5609.
   PubMed Web of Science ®Google Scholar
• GEDIK, C. M., EWEN, S. W. B., and COLLINS, A. R., 1992, Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells. International Journal of Radiation Biology, 62, 313–320.
   PubMed Web of Science ®Google Scholar
• GREEN, M. H. L., ARLETT, C. F., COLE, J., HARCOURT, S. A., PRIESTLEY, A., WAUGH, A. P. W., STEPHENS, G., BEARE, D. M., BROWN, N. A. P., and SHUN-SHIN, G. A., 1991, Comparative human cellular radiosensitivity. III: y-Radiation survival of cul-tured skin fibroblasts and resting T-lymphocytes from the peripheral blood of the same individual. International Journal of Radiation Biology, 59, 749–765.
   Google Scholar
• GREEN, M. H. L., LOWE, J. E., HARCOURT, S. A., AKINLUYI, p., ROWE, T., COLE, J., ANSTEY, A. V., and ARLETT, C. F., 1992, UV-C sensitivity of unstimulated and stimulated human lym-phocytes from normal and xeroderma pigmentosum donors in the comet assay: a potential diagnostic technique. Mutation Research, 273, 137–144.
   PubMed Web of Science ®Google Scholar
• GREEN, M. H. L., LOWE, J. E., WAUGH, A. P. W., ALDRIDGE, K. E., COLE, J., and ARLETT, C. F., 1994, Effect of diet and vitamin C on DNA strand breakage in freshly-isolated human white blood cells. Mutation Research, 316, 91–102.
   PubMed Web of Science ®Google Scholar
• IARG Monographs on the Evaluation of Carcinogenic Risks to Humans, 1992, Solar and Ultraviolet Radiation, vol. 55 (IARC, Lyon).
   Google Scholar
• KEYSE, S. M., MOSS, S. H., and DAVIES, D. J. G., 1983, Action spectra for inactivation of normal and xeroderma pigmentosum human skin fibroblasts by ultraviolet radiations. Photochemistry and Photobiology, 37, 307–312.
   PubMed Web of Science ®Google Scholar
• KRAEMER, G. F., LEE, M. M., and SCOTTO, J., 1987, Xeroderma pigmentosum. Cutaneous, ocular and neurologic abnormalities in 830 published cases. Archives of Dermatology, 123, 241–250.
   PubMed Web of Science ®Google Scholar
• LEHMANN, A. R., ARLETT, C. F., BROUGHTON, B. C., HARCOURT, S. A., STEINGRIMSDOTTIR, H., STEFANINI, M., TAYLOR, A. M. R., NATARAJAN, A. T., GREEN, S., KING, M. D., MACKIE, R. M., STEPHENSON, J. B. P., and ToLmIE, J. L., 1988, Trichothio-dystrophy, a human DNA repair disorder with hetero-geneity in the cellular response to ultraviolet light. Cancer Research, 48, 6090–6096.
   PubMedGoogle Scholar
• LEHMANN, A. R., and NORRIS, P. G., 1989, DNA repair and cancer: speculations based on studies with xeroderma pigmentosum, Cockayne's syndrome and trichothio-dystrophy. Carcinogenesis, 10, 1353–1356.
   PubMed Web of Science ®Google Scholar
• MADRONICH, S., and de GRUIJL, F. R., 1993, Skin cancer and UV radiation. Nature, 366, 23.
   PubMed Web of Science ®Google Scholar
• MADRONICH, S., and de GRUIJL, F. R., 1994, Stratospheric ozone depletion between 1979 and 1992: implications for biologically active ultraviolet-B radiation and non-mel-anoma skin cancer incidence. Photochemistry and Photobiology, 59, 541–546.
   PubMed Web of Science ®Google Scholar
• MAHER, V., and McCoKmicK, J. J., 1976, Effect of DNA repair on the cytotoxicity and mutagenicity of UV irradiation and of chemical carcinogens in normal and xeroderma pigmentosum cells. In: Biology of Radiation Carcinogen-esis. Edited by: J. M. Yuhas, R. W. Tennant and J. B. Regan (New York, Raven), pp. 129–145.
   Google Scholar
• MCKELVEY-MARTIN, V. J., GREEN, M. H. L., SCHMEZER, p. POOL-ZOBEL, B. L., DE MEO, M. P., and COLLINS, A., 1993, The single cell gel electrophoresis assay (comet assay): a european review. Mutation Research, 288, 47–63.
   Google Scholar
• MITCHELL, D. L., HAIPEK, C. A., and CLARKSON, J. M., 1985, (6-4) photoproducts are removed from the DNA of UV-irradiated mammalian cells more efficiently than cyclo-butane pyrimidine dimers. Mutation Research, 143, 109–112.
   PubMed Web of Science ®Google Scholar
• MONDELLO, C., NARDO, T., GILIANI, S., ARRAND, J. E., WEBER, C. A., LEHMANN, A. R., Nuzzo, F., and STEFANINI, M., 1994, Molecular analysis of the XP-D gene in Italian families with patients affected by trichothiodystrophy and xer-oderma pigmentosum group D. Mutation Research, 314, 159–165.
   PubMedGoogle Scholar
• NAKAZAWA, H., ENGLISH, D., RANDELL, P. F., NAKAZAWA, K., MARTEL, N., ARMSTRONG, B. K., and YAMASAKI, H., 1994, UV and skin cancer: specific p53 gene mutation in normal skin as a biologically relevant exposure measurement. Pro-ceedings of the National Academy of Sciences, USA, 91, 360–364.
   PubMed Web of Science ®Google Scholar
• OLIVE, P. L., BANATH, J. P., and DURAND, R. E., 1990, Hetero-geneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the 'comet' assay. Radiation Research, 122, 86–94.
   PubMed Web of Science ®Google Scholar
• OLIVE, P. L., and BANATH, J. P., 1993, Induction and rejoining of radiation-induced DNA single-strand breaks: 'tail moment' as a function of position in the cell cycle. Mutation Research, 294, 275–283.
   PubMed Web of Science ®Google Scholar
• PEAK, J. G., PILAS, B., DUDEK, E. J., and PEAR, M. J., 1991, DNA breaks caused by monochromatic 365 nm ultraviolet-A radiation or hydrogen peroxide and their repair in human epithelioid and xeroderma pigmentosum cells. Photochemistry and Photobiology, 54, 197–203.
   PubMed Web of Science ®Google Scholar
• PEAK, M. J., PEAK, J. G., and CARNES, B. A., 1987, Induction of direct and indirect single-strand breaks in human cell DNA by far- and near-ultraviolet radiations: action spectrum and mechanisms. Photochemistry and Photo-biology, 45, 381–387.
   PubMed Web of Science ®Google Scholar
• RfiNGER, T. M., EPE, B., and MOLLER, K., 1995, Repair of ultraviolet B and singlet oxygen-induced DNA damage in xeroderma pigmentosum cells. Journal of Investigative Dermatology, 104, 68–73
   PubMedGoogle Scholar
• SAG EE., 1993, Distribution and repair of photolesions in DNA: genetic consequences and the role of sequence context. Photochemistry and Photobiology, 57, 163–174.
   PubMedGoogle Scholar
• SARASIN, A., 1991, The paradox of DNA repair-deficient diseases. Cancer Journal, 4, 233–237.
   Google Scholar
• SATO, M., NISHIGORI, C., LU, Y, ZGHAL, M., YAGI, T., and TAKEBE, H., 1994, Far less frequent mutations in ras genes than in the p53 gene in skin tumors of xeroderma pigmen-tosum patients. Molecular Carcinogenesis, 11, 98–105.
   PubMed Web of Science ®Google Scholar
• SETLOW, R. B., and WOODHEAD, A. D., 1994, Temporal changes in the incidence of malignant melanoma: explanation from action spectra. Mutation Research, 307, 365–374.
   PubMed Web of Science ®Google Scholar
• SINGH, N. P., McCoy, M. T., TICE, R. R., and SCHNEIDER, E. L., 1988, A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175, 184–191.
   PubMed Web of Science ®Google Scholar
• SINGH, N. P., TICE, R. R., STEPHENS, R. E. and SCHNEIDER, E. L., 1991, A microgel electrophoresis technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides. Mutation Research, 252, 289–296.
   PubMed Web of Science ®Google Scholar
• SMITH, P. J., and PATERSON, M. C., 1982, Lethality and the induction and repair of DNA damage in far, mid or near UV-irradiated human fibroblasts: comparison of effects in normal, xeroderma pigmentosum and Bloom's syndrome cells. Photochemistry and Photobiology, 36, 333–343.
   PubMed Web of Science ®Google Scholar
• STEFANINI, M., LAGOMARSINI, P., GILIANI, S., NARDO, T., BOTTA, E., PESERICO, A., KLEIHJER, W. J., LEHMANN, A. R., and SARASIN, A., 1993, Genetic heterogeneity of the excision repair defect associated with trichothiodystrophy. Carcino-genesis, 14, 1101–1105.
   PubMedGoogle Scholar
• TICE, R. R., ANDREWS, P. W., HIRAI, 0., and SINGH, N. P., 1991, The single cell gel (SCG) assay: an electrophoretic technique for the detection of DNA damage in indivi-dual cells. Advances in Experimental Medicine Biology, 283, 157–164.
   Google Scholar
• TERRELL, R. M., and KEYSE, S. M., 1990, New trends in photo-biology. The interaction of UVA radiation with cul-tured cells. Journal of Photochemistry and Photobiology, Part B: Biology, 4, 349–361.
   Google Scholar
• VUILLAUME, M., DAYA-GROSJEAN, L., VINCENS, 13. PENNETIER, J. L., TARROUX, p. BARET, A., CALVAYRAC, R., TAIEB, A., and SARASIN, A., 1992, Striking differences in cellular catalase activity between two DNA repair-deficient dis-eases: xeroderma pigmentosum and trichothio-dystrophy. Carcinogenesis, 13, 321–328.
   Google Scholar
• WEEDA, G., and HOEIJMAKERS, J. H. J., 1993, Genetic analysis of nucleotide excision repair in mammalian cells. Semi-nars in Cancer Biology, 4, 105–117.
   PubMed Web of Science ®Google Scholar
• WHITAKER, S. J., POWELL, S. N., and MCMILLAN, T. J., 1991, Molecular assays of radiation-induced DNA damage. European Journal of Cancer, 27, 922–928.
   PubMed Web of Science ®Google Scholar
• DE WITH, A., LEITZ, G., and GRUELICH, K. 0., 1994, UV-B laser-induced DNA damage in lymphocytes observed by single-cell gel electrophoresis. Journal of Photochemistry and Photobiology, Part B: Biology, 24, 47–53.
   Google Scholar