Determining sample size adequacy for animal model studies in nutrition research: limits and ethical challenges of ordinary power calculation procedures

References

• Antonioli L, Caputi V, Fornai M, Pellegrini C, Gentile D, Giron MC, Orso G, Bernardini N, Segnani C, Ippolito C, et al. 2019. Interplay between colonic inflammation and tachykininergic pathways in the onset of colonic dysmotility in a mouse model of diet-induced obesity. Int J Obes. 43:331–343.
   Google Scholar
• Béliard S, Le Goff W, Saint-Charles F, Poupel L, Deswaerte V, Bouchareychas L, Huby T, Lesnik P. 2017. Modulation of Gr1low monocyte subset impacts insulin sensitivity and weight gain upon high-fat diet in female mice. Int J Obes. 41(12):1805.
   Web of Science ®Google Scholar
• Button KS, Ioannidis JPA, Mokrysz C, Nosek BA, Flint J, Robinson ESJ, Munafò MR. 2013. Power failure: why small sample size undermines the reliability of neuroscience. Nat Rev Neurosci. 14(5):365.
   PubMed Web of Science ®Google Scholar
• Charan J, Biswas T. 2013. How to calculate sample size for different study designs in medical research? Indian J Psychol Med. 35(2):121.
   PubMedGoogle Scholar
• Cohen J. 1988. Statistical power analysis for the behavioral sciences. 2nd ed. Hillsdale: Erlbaum Associates.
   Google Scholar
• Diet CB. 1977. Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. J Nutr. 107:1340.
   PubMed Web of Science ®Google Scholar
• Domenichiello AF, Kitson AP, Metherel AH, Chen CT, Hopperton KE, Stavro PM, Bazinet RP. 2017. Whole-body docosahexaenoic acid synthesis-secretion rates in rats are constant across a large range of dietary α-linolenic acid intakes–3. J Nutr. 147(1):37–44.
   PubMed Web of Science ®Google Scholar
• Dominguez-Uscanga A, Loarca-Piña G, Gonzalez de Mejia E. 2017. Baked corn (Zea mays L.) and bean (Phaseolus vulgaris L.) snack consumption lowered serum lipids and differentiated liver gene expression in C57BL/6 mice fed a high-fat diet by inhibiting PPARγ and SREBF2. J Nutr Biochem. 50:1–15.
   PubMed Web of Science ®Google Scholar
• Fay MP, Proschan MA. 2010. Wilcoxon-Mann-Whitney or t-test? On assumptions for hypothesis tests and multiple interpretations of decision rules. Statist Surv. 4(0):1.
   PubMedGoogle Scholar
• Ferdowsian HR, Beck N. 2011. Ethical and scientific considerations regarding animal testing and research. PLoS One. 6(9):e24059.
   PubMed Web of Science ®Google Scholar
• Festing MF, Altman DG. 2002. Guidelines for the design and statistical analysis of experiments using laboratory animals. ILAR J. 43(4):244–258.
   PubMedGoogle Scholar
• Gali Ramamoorthy T, Allen TJ, Davies A, Harno E, Sefton C, Murgatroyd C, White A. 2018. Maternal overnutrition programs epigenetic changes in the regulatory regions of hypothalamic Pomc in the offspring of rats. Int J Obes. 42:1431–1444.
   Web of Science ®Google Scholar
• Gao X, Xiao Z-H, Liu M, Zhang N-Y, Khalil MM, Gu C-Q, Qi D-S, Sun L-H. 2018. Dietary silymarin supplementation alleviates zearalenone-induced hepatotoxicity and reproductive toxicity in rats. J Nutr. 148(8):1209–1216.
   PubMed Web of Science ®Google Scholar
• Gibbons JD, Chakraborti S. 1991. Comparisons of the Mann-Whitney, Student’s t, and alternate t tests for means of normal distributions. J Exp Educ. 59(3):258–267.
   Web of Science ®Google Scholar
• Hackam DG, Redelmeier DA. 2006. Translation of research evidence from animals to humans. JAMA. 296(14):1727–1732.
   Web of Science ®Google Scholar
• Hart A. 2001. Mann-Whitney test is not just a test of medians: differences in spread can be important. BMJ. 323(7309):391.
   PubMed Web of Science ®Google Scholar
• Haygert P, Roversi K, Milanesi LH, Maurer LH, Camponogara C, Duarte T, Barcelos RCS, Emanuelli T, Oliveira SM, Duarte MMMF, et al. 2018. Can the dietary fat type facilitate memory impairments in adulthood? A comparative study between Mediterranean and Western-based diet in rats. J Nutr Biochem. 59:104–113.
   PubMed Web of Science ®Google Scholar
• Heying EK, Ziemer KL, Tanumihardjo JP, Palacios-Rojas N, Tanumihardjo SA. 2018. β-cryptoxanthin–biofortified hen eggs enhance vitamin A status when fed to male Mongolian Gerbils. J Nutr. 148(8):1236–1243.
   PubMed Web of Science ®Google Scholar
• Kilkenny C, Browne WJ, Cuthill IC, Emerson M, Altman DG. 2010. Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research. PLoS Biol. 8(6):e1000412.
   PubMed Web of Science ®Google Scholar
• Krejcie RV, Morgan DW. 1970. Determining sample size for research activities. Educ Psychol Meas. 30(3):607–610.
   Web of Science ®Google Scholar
• Kyung DS, Sung HR, Kim YJ, Kim KD, Cho SY, Choi JH, Lee Y-H, Kim IY, Seong JK. 2018. Global transcriptome analysis identifies weight regain-induced activation of adaptive immune responses in white adipose tissue of mice. Int J Obes. 42(4):755.
   Web of Science ®Google Scholar
• Li W, Tang R, Ma F, Ouyang S, Liu Z, Wu J. 2018. Folic acid supplementation alters the DNA methylation profile and improves insulin resistance in high-fat diet-fed mice. J Nutr Biochem. 59:76–83.
   PubMed Web of Science ®Google Scholar
• Morse JM. 2000. Determining sample size. Thousand Oaks, CA: Sage Publications.
   Google Scholar
• Murphy MO, Herald JB, Leachman J, Villasante TA, Cohn DM, Loria AS. 2018. A model of neglect during postnatal life heightens obesity-induced hypertension and is linked to a greater metabolic compromise in female mice. Int J Obes. 42:1354–1365.
   Web of Science ®Google Scholar
• Nakagawa S, Cuthill IC. 2007. Effect size, confidence interval and statistical significance: a practical guide for biologists. Biol Rev. 82(4):591–605.
   PubMed Web of Science ®Google Scholar
• Noble EE, Hsu TM, Jones RB, Fodor AA, Goran MI, Kanoski SE. 2017. Early-life sugar consumption affects the rat microbiome independently of obesity–3. J Nutr. 147(1):20–28.
   PubMed Web of Science ®Google Scholar
• Parasuraman S. 2011. Toxicological screening. J Pharmacol Pharmacother. 2(2):74.
   PubMedGoogle Scholar
• Poncet A, Courvoisier DS, Combescure C, Perneger TV. 2016. Normality and sample size do not matter for the selection of an appropriate statistical test for two-group comparisons. Methodology. (2):61:12.
   Web of Science ®Google Scholar
• Poret JM, Souza-Smith F, Marcell SJ, Gaudet DA, Tzeng TH, Braymer HD, Harrison-Bernard LM, Primeaux SD. 2018. High fat diet consumption differentially affects adipose tissue inflammation and adipocyte size in obesity-prone and obesity-resistant rats. Int J Obes. 42(3):535.
   Web of Science ®Google Scholar
• Prescott MJ, Lidster K. 2017. Improving quality of science through better animal welfare: the NC3Rs strategy. Lab Anim. 46(4):152.
   Web of Science ®Google Scholar
• Razali NM, Wah YB. 2011. Power comparisons of shapiro-wilk, kolmogorov-smirnov, lilliefors and anderson-darling tests. J Stat Model Anal. 2(1):21–33.
   Google Scholar
• Scott S, Kranz JE, Cole J, Lincecum JM, Thompson K, Kelly N, Bostrom A, Theodoss J, Al-Nakhala BM, Vieira FG, et al. 2008. Design, power, and interpretation of studies in the standard murine model of ALS. Amyotroph Lateral Scler. 9(1):4–15.
   PubMed Web of Science ®Google Scholar
• Singh A, Zapata RC, Pezeshki A, Reidelberger RD, Chelikani PK. 2018. Inulin fiber dose-dependently modulates energy balance, glucose tolerance, gut microbiota, hormones and diet preference in high-fat fed male rats. J Nutr Biochem. 59:142–152.
   PubMed Web of Science ®Google Scholar
• van der Worp HB, Howells DW, Sena ES, Porritt MJ, Rewell S, O'Collins V, Macleod MR. 2010. Can animal models of disease reliably inform human studies? PLoS Med. 7(3):e1000245.
   PubMed Web of Science ®Google Scholar
• Zhou M-M, Ding L, Wen M, Che H-x, Huang J-Q, Zhang T-T, Xue C-h, Mao X-Z, Wang Y-M. 2018. Mechanisms of DHA-enriched phospholipids in improving cognitive deficits in aged SAMP8 mice with high-fat diet. J Nutr Biochem. 59:64–75.
   PubMed Web of Science ®Google Scholar
• Zimmerman DW, Zumbo BD. 1993. Relative power of the Wilcoxon test, the Friedman test, and repeated-measures ANOVA on ranks. J Exp Educ. 62(1):75–86.
   Web of Science ®Google Scholar