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Original Articles

Light-dependent utilization of two radiolabelled carbon sources, sodium bicarbonate and sodium acetate, and relationships with long chain polyunsaturated fatty acid synthesis in the microalga Pavlova lutheri (Haptophyta)

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Pages 143-152 | Received 09 Jul 2010, Accepted 15 Mar 2011, Published online: 23 May 2011

References

  • Apt , KE and Behrens , PW . 1999 . Commercial developments in microalgal biotechnology . J. Phycol. , 35 : 215 – 226 .
  • Beardall , J , Burger-Wiersma , T , Rijkeboer , M , Sukenik , A , Lemoalle , J , Dubinsky , Z and Tontvielle , D . 1994 . Studies on enhanced post-illumination respiration in microalgae . J. Plankton Res. , 16 : 1401 – 1410 .
  • Blanchemain , A and Grizeau , D . 1996 . Eicosapentaenoic acid content of Skeletonema costatum as a function of growth and irradiance; relation with chlorophyll a content and photosynthetic capacity . J. Exp. Mar. Biol. Ecol. , 196 : 177 – 188 .
  • Bligh , EG and Dyer , WJ . 1959 . A rapid method of lipid extraction and purification . Can. J. Biochem. Phys. , 37 : 911 – 917 .
  • Brown , MR , Dunstan , GA , Jeffrey , SW , Volkman , JK , Barrett , SM and Le Roi , JM . 1993 . The influence of irradiance on the biochemical composition of the prymnesiophyte Isochrysis sp. (Clone T-ISO) . J. Phycol. , 29 : 601 – 612 .
  • Browse , J and Somerville , C . 1991 . Glycerolipid synthesis: biochemistry and regulation . Ann. Rev. Plant Physiol. , 42 : 467 – 506 .
  • Cadoret , JP and Bernard , O . 2008 . La production de biocarburant lipidique avec des microalgues: promesses et défis . J. Soc. Biol. , 202 : 201 – 211 .
  • Cardozo , KHM , Guaratini , T , Barros , MP , Falcao , VR , Tonon , AP , Lopes , NP , Campos , S , Torres , MA , Souza , AO , Colepicolo , P and Pinto , E . 2007 . Metabolites from algae with economical impact . Comp. Biochem. Phys. C , 146 : 60 – 78 .
  • Carvalho , AP and Malcata , FX . 2000 . Effect of culture media on production of polyunsaturated fatty acids by Pavlova lutheri . Cryptogamie Algol. , 21 : 59 – 71 .
  • Carvalho , AP , Pontes , I , Gaspar , H and Macalta , FX . 2006 . Metabolic relationships between macro- and micronutrients, and the eicosapentaenoic acid and docosahexaenoic acid contents of Pavlova lutheri . Enzyme Microb. Tech. , 38 : 358 – 366 .
  • Chisti , Y . 2007 . Biodiesel from microalgae . Biotech. Adv. , 25 : 294 – 306 .
  • De Brouwer , JFC , Wolfstein , K and Stal , LJ . 2002 . Physical characterization and diel dynamics of different fractions of extracellular polysaccharides in an axenic culture of a benthic diatom . Eur. J. Phycol. , 37 : 37 – 44 .
  • Doughman , SD , Krupanidhi , S and Sanjeevi , CB . 2007 . Omega-3 fatty acids for nutrition and medicine: considering microalgae oil as a vegetarian source of EPA and DHA . Curr. Diabetes Rev. , 3 : 198 – 203 .
  • Dyerberg , J and Jorgensen , KA . 1982 . Marine oils and thrombogenesis . Prog. Lipid Res. , 21 : 255 – 269 .
  • Eichenberger , W and Gribi , C . 1997 . Lipids of Pavlova lutheri: Cellular site and metabolic role of DGCC . Phytochemistry , 45 : 1561 – 1567 .
  • Guihéneuf , F , Mimouni , V , Ulmann , L and Tremblin , G . 2008 . Environmental factors affecting growth and omega 3 fatty acid composition in Skeletonema costatum. The influences of irradiance and carbon source . Diatom Res. , 23 : 93 – 103 .
  • Guihéneuf , F , Mimouni , V , Ulmann , L and Tremblin , G . 2009 . Combined effects of irradiance level and carbon source on fatty acid and lipid class composition in the microalga Pavlova lutheri commonly used in mariculture . J. Exp. Mar. Biol. Ecol. , 369 : 136 – 143 .
  • Harrison , PJ , Waters , RE and Taylor , FJR . 1980 . A broad spectrum artificial seawater medium for coastal and open ocean phytoplankton . J. Phycol. , 16 : 28 – 35 .
  • Harwood , JL and Jones , AL . 1989 . Lipid metabolism in algae . Adv. Bot. Res. , 16 : 1 – 52 .
  • Henderson , RJ and Tocher , DT . 1992 . “ Thin-layer chromatography ” . In Lipid Analysis – A Practical Approach , Edited by: Hamilton , RJ and Hamilton , S . 65 – 111 . Oxford : Oxford University Press .
  • Hunter , SC and Ohlrogge , JB . 1998 . Regulation of spinach chloroplast acetyl-CoA carboxylase . Arch. Biochem. Biophys. , 359 : 170 – 178 .
  • Ke , J , Behal , RH , Back , SL , Nikolau , BJ , Wurtele , ES and Oliver , DJ . 2000 . The role of pyruvate dehydrogenase and acetyl-coenzyme A synthetase in fatty acid synthesis in developing Arabidopsis seeds . Plant Physiol. , 123 : 497 – 508 .
  • Khotimchenko , SV and Yakovleva , IM . 2005 . Lipid composition of the red alga Tichocarpus crinitus exposed to different levels of photon irradiance . Phytochemistry , 66 : 73 – 79 .
  • Khozin , I , Adlerstein , D , Bigogno , C , Heimer , YM and Cohen , Z . 1997 . Elucidation of the biosynthesis of eicosapentaenoic acid in the microalga Porphyridium cruentum . Plant Physiol. , 114 : 223 – 230 .
  • Khozin-Goldberg , I , Didi-Cohen , S , Shayakhmetova , I and Cohen , Z . 2002 . Biosynthesis of eicosapentaenoic acid (EPA) in the freshwater eustigmatophyte Monodus subterraneus (Eustigmatophyceae) . J. Phycol. , 38 : 745 – 756 .
  • Kispal , G , Cseko , J , Alkonyi , I and Sandor , A . 1991 . Isolation and characterization of carnitine acetyltransferase from S. cerevisiae . Biochim. Biophys. Acta , 1085 : 217 – 222 .
  • Klein , HP and Jahnke , L . 1971 . Variations in the localization of acetyl-coenzyme A synthetase in aerobic yeast cells . J. Bacteriol. , 106 : 596 – 602 .
  • Klyachko-Gurvich , GL , Tsoglin , LN , Doucha , J , Kopetskii , J , Shebalina , JB and Semenenko , VE . 1999 . Desaturation of fatty acids as an adaptive response to shifts in light intensity . Physiol. Plant. , 107 : 240 – 249 .
  • Lafarga-De La Cruz , F , Valenzuela-Espinoza , E , Millan-Nunez , R , Trees , CC , Santamaria-Del-Angel , E and Nunez-Cebrero , F . 2006 . Nutrient uptake, chlorophyll a and carbon fixation by Rhodomonas sp. (Cryptophyceae) cultured at different irradiance and nutrient concentrations . Aquacult. Eng. , 35 : 51 – 60 .
  • Lagarde , M , Croset , M , Sicard , B and Dechavanne , M . 1986 . Biological activities and metabolism of eicosapentaenoic acids in relation to platelet and endothelial function . Prog. Lipid Res. , 25 : 269 – 271 .
  • Lee , YK . 2004 . “ Algal nutrition – Heterotrophic carbon nutrition ” . In Handbook of Microalgal Culture: Biotechnology and Applied Phycology , Edited by: Richmond , A . 116 – 124 . Oxford : Blackwell Publishing .
  • Leonardos , N and Lucas , IAN . 2000 . The nutritional value of algae grown under different culture conditions for Mytilus edulis L. larvae . Aquaculture , 182 : 301 – 315 .
  • Masojidek , J , Kopecky , J , Koblizek , M and Torzillo , G . 2004 . The xanthophyll cycle in green algae (Chlorophyta): its role in the photosynthetic apparatus . Plant Biol. , 6 : 342 – 349 .
  • Mimouni , V , Ulmann , L , Tremblin , G and Robert , JM . 2003 . Desaturation of linoleic acid in the marine diatom Haslea ostrearia Simonsen (Bacillariophyceae) . Cryptogamie Algol. , 24 : 269 – 276 .
  • Morant-Manceau , A , Nguyen , TLN , Pradier , E and Tremblin , G . 2007 . Carbonic anhydrase and photosynthesis in marine diatoms . Eur. J. Phycol. , 42 : 263 – 270 .
  • Moreno , VJ , De Moreno , JEA and Brenner , RR . 1979 . Biosynthesis of unsaturated fatty acids in the diatom Phaeodactylum tricornutum . Lipids , 14 : 15 – 19 .
  • Mouget , JL , Tremblin , G , Manceau , A , Morancais , M and Robert , JM . 1999 . Long-term photoacclimation of Haslea ostrearia (Bacillariophyta): effect of irradiance on growth rates, pigment content and photosynthesis . Eur. J. Phycol. , 34 : 109 – 115 .
  • Narce , M , Gresti , J and Bezard , J . 1988 . Method for evaluating the bioconversion of radioactive polyunsaturated fatty acids by use of reversed-phase liquid chromatography . J. Chromatogr. A , 448 : 249 – 264 .
  • Ohlrogge , JB and Browse , J . 1995 . Lipid biosynthesis . Plant Cell , 7 : 957 – 970 .
  • Perkins , RG , Mouget , JL , Lefebvre , S and Lavaud , J . 2006 . Light response curve methodology and possible implications in the application of chlorophyll fluorescence to benthic diatoms . Mar. Biol. , 149 : 703 – 712 .
  • Ponis , E , Parisi , G , Le Coz , JR , Zitelli , GC and Tredici , MR . 2006a . Effect of the culture system and culture technique on biochemical characteristics of Pavlova lutheri and its nutritional value for Crassostrea gigas larvae . Aquacult. Nut. , 12 : 322 – 329 .
  • Ponis , E , Probert , I , Veron , B , Le Coz , JR , Mathieu , M and Robert , R . 2006b . Nutritional value of six Pavlovophyceae for Crassostrea gigas and Pecten maximus larvae . Aquaculture , 254 : 544 – 553 .
  • Post-Beittenmiller , D , Roughan , PG and Ohlrogge , JB . 1991 . In vivo pools of free and acylated carrier proteins in spinach: evidence for sites of regulation of fatty acid biosynthesis . J. Biol. Chem. , 266 : 1858 – 1865 .
  • Post-Beittenmiller , D , Roughan , PG and Ohlrogge , JB . 1992 . Regulation of plant fatty acid biosynthesis: analysis of acyl-CoA and acyl-ACP substrate pools in spinach and pea chloroplasts . Plant Physiol. , 100 : 923 – 930 .
  • Raven , JA and Beardall , J . 2003 . “ CO2 acquisition mechanisms of algae: carbon dioxide diffusion and carbon dioxide concentrating mechanisms ” . In Photosynthesis in the Algae , Edited by: Larkum , A , Raven , JA and Douglas , S . 225 – 244 . Dordrecht : Kluwer .
  • Rawsthorme , S . 2002 . Carbon flux and fatty acid synthesis in plants . Prog. Lipid Res. , 41 : 182 – 196 .
  • Rech , M , Morant-Manceau , A and Tremblin , G . 2008 . Carbon fixation and carbonic anhydrase activity in Haslea ostrearia (Bacillariophyceae) in relation to growth irradiance . Photosynthetica , 46 : 52 – 62 .
  • Rivkin , RB . 1989 . Influence of irradiance and spectral quality on the carbon metabolism of phytoplankton. I. Photosynthesis, chemical composition and growth . Mar. Ecol. Prog. Ser. , 55 : 291 – 304 .
  • Rousseau , D , Helies-Toussaint , C , Moreau , D , Raederstorff , D and Gryenberg , A . 2003 . Dietary n-3 PUFAs affect the blood pressure rise and cardiac impairments in a hyperinsulinemia rat model in vivo . Am. J. Physiol. Heart Circ. Physiol. , 285 : H1294 – H1302 .
  • Schneider , JC and Roessler , P . 1994 . Radiolabelling studies of lipids and fatty acids in Nannochloropsis (Eustigmatophyceae), an oleaginous marine alga . J. Phycol. , 30 : 594 – 598 .
  • Slover , HT and Lanza , E . 1979 . Quantitative analysis of food fatty acids by capillary gas chromatography . J. Am. Oil Chem. Soc. , 56 : 933 – 943 .
  • Smith , REH and D'Souza , FML . 1993 . Macromolecular labelling patterns and inorganic nutrient limitation of a north Atlantic spring bloom . Mar. Ecol. Prog. Ser. , 92 : 111 – 118 .
  • Spolaore , P , Joannis-Cassan , C , Duran , E and Isambart , A . 2006 . Commercial applications of microalgae . J. Biosci. Bioeng. , 101 : 87 – 96 .
  • Sukenik , A , Carmeli , Y and Berner , Y . 1989 . Regulation of fatty acid composition by irradiance level in the Eustigmatophyceae Nannochloropsis sp . J. Phycol. , 25 : 686 – 692 .
  • Sukenik , A , Zmora , O and Carmeli , Y . 1993 . Biochemical quality of marine unicellular algae with special emphasis on lipid composition: II . Nannochloropsis sp. Aquaculture , 117 : 313 – 326 .
  • Tatsuzawa , H and Takizawa , E . 1995 . Changes in lipid and fatty acid composition of Pavlova lutheri . Phytochemistry , 40 : 397 – 400 .
  • Thompson , PA , Guo , MX and Harrison , PJ . 1996 . Nutritional value of diets that vary in fatty acid composition for larval Pacific oysters (Crassostrea gigas) . Aquaculture , 143 : 379 – 391 .
  • Thompson , PA , Harrison , PJ and White , JNC . 1990 . Influence of irradiance on the fatty acid composition of phytoplankton . J. Phycol. , 26 : 278 – 288 .
  • Tremblin , G and Robert , JM . 2001 . Carbon fixation by the peculiar marine diatom Haslea ostrearia . Photosynthetica , 39 : 215 – 220 .
  • Tremblin , G , Cannuel , R , Mouget , JL , Rech , M and Robert , JM . 2000 . Change in light quality due to a blue-green pigment, mareninne, released in oyster-ponds: effect on growth and photosynthesis in two diatoms, Haslea ostrearia and Skeletonema costatum . J. Appl. Phycol. , 12 : 557 – 566 .
  • Tzovenis , I , De Pauw , N and Sorgeloos , P . 2003 . Optimisation of T-ISO biomass production rich in essential fatty acids: I. Effect of different light regimes on growth and biomass production . Aquaculture , 216 : 203 – 222 .
  • Ward , OP and Singh , A . 2005 . Omega-3/6 fatty acids: alternative sources of production . Process Biochem. , 40 : 3627 – 3652 .
  • Zeiher , CA and Randall , DD . 1991 . Spinach leaf acetyl-coenzyme A synthetase: purification and characterization . Plant Physiol. , 96 : 382 – 389 .

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