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British Journal of Biomedical Science Volume 64, 2007 - Issue 2
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Biomedical Science in Brief

Use of heat provides a fast and efficient way to undertake melanin bleaching with dilute hydrogen peroxide

G.E. OrchardDepartment of Histopathology, St John’s Institute of Dermatology, St Thomas’ Hospital, London, SE1 7EHCorrespondence[email protected]
Pages 89-91 | Published online: 12 Feb 2018

References

  • Fleming MG, Bergfeld WF. A simple immunohistochemical technique for distinguishing melanocytes and melanophages in paraffin- embedded tissue. J Cutan Pathol 1990; 17: 77–81.
  • Kamino H, Tam ST. Immunoperoxidase technique modified by counterstain with azure B as a diagnostic aid in evaluating heavily pigmented melanocytic neoplasms. J Cutan Pathol 1991; 18: 436–9.
  • McGovern J, Crocker J. The effect of melanin pigment removal on the peroxidase-antiperoxidase immunoperoxidase technique. Am J Clin Pathol 1987; 88: 480–3.
  • Kivela. T. Immunohistochemical staining followed by bleaching of melanin: a practical method for ophthalmic pathology (Letter). Br J Biomed Sci 1995; 52: 325.
  • Foss.AJE. Immunohistocehmical staining followed by bleaching of melanin: a practical method for ophthalmic pathology (Reply). Br J Biomed Sci 1995; 52: 326.
  • Orchard GE, Calonje E. Immunohistochemical staining following the melanin bleaching technique in an atypical junctional melanocytic lesion with prominent dermal melanosis (Letter). Br J Biomed Sci 1997; 54: 74–5.
  • Orchard GE, Calonje E. The effect of melanin bleaching on immunohistochemical staining in heavily pigmented melanocytic neoplasms. Am J Dermatopathol 1998; 20: 357–61.
  • Orchard GE. Heavily pigmented melanocytic neoplasms: comparison of two melanin-bleaching techniques and subsequent immunohistochemical staining. Br J Biomed Sci 1999; 56: 188–93.

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