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Original Articles

Application of RT-Bst to enhance detection of pathogenic viruses of the respiratory tract

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Pages 128-134 | Accepted 10 May 2015, Published online: 04 Jan 2016

References

  • Antonishyn NA, Levett PN. Molecular diagnostic assays for detection of viral respiratory pathogens in institutional outbreaks. Mol Diagn Ther 2010; 14 (5): 283–93.
  • Beck ET, Henrickson KJ. Molecular diagnosis of respiratory viruses. Future Microbiol 2010; 5 (6): 901–16.
  • Choudhary ML, Anand SP, Heydari M et al. Development of a multiplex one step RT-PCR that detects eighteen respiratory viruses in clinical specimens and comparison with real time RT- PCR. J Virol Methods 2013; 189 (1): 15–9.
  • Eugene-Ruellan G, Freymuth F, Bahloul C, Badrane H, Vabret A, Tordo N. Detection of respiratory syncytial virus A and B and parainfluenza virus 3 sequences in respiratory tracts of infants by a single PCR with primers targeted to the L-polymerase gene and differential hybridization. J Clin Microbiol 1998; 36 (3): 796–801.
  • Freymuth F, Vabret A, Galateau-Salle F et al. Detection of respiratory syncytial virus, parainfluenza virus 3, adenovirus and rhinovirus sequences in respiratory tract of infants by polymerase chain reaction and hybridization. Clin Diagn Virol 1997; 8 (1): 31–40.
  • Olofsson S, Brittain-Long R, Andersson LM, Westin J, Lindh M. PCR for detection of respiratory viruses: seasonal variations of virus infections. Expert Rev Anti Infect Ther 2011; 9 (8): 615–26.
  • Turner RB. The common cold. Pediatr Ann 1998; 27 (12): 790–5.
  • Arruda E, Pitkäranta A, Witek TJ Jr, Doyle CA, Hayden FG. Frequency and natural history of rhinovirus infections in adults during autumn. J Clin Microbiol 1997; 35 (11): 2864–8.
  • Johnston SL, Sanderson G, Pattemore PK et al. Use of polymerase chain reaction for diagnosis of picornavirus infection in subjects with and without respiratory symptoms. J Clin Microbiol 1993; 31 (1): 111–7.
  • Pappas DE, Hendley JO, Hayden FG, Winther B. Symptom profile of common colds in school-aged children. Pediatr Infect Dis J 2008; 27 (1): 8–11.
  • Eccles R. Mechanisms of symptoms of the common cold and influenza. Br J Hosp Med (Lond) 2007; 68 (2): 71–5.
  • Pizzichini MM, Pizzichini E, Efthimiadis A et al. Asthma and natural colds. Inflammatory indices in induced sputum: a feasibility study Am J Respir Crit Care Med 1998; 158 (4): 1178–84.
  • Mahony J, Chong S, Merante F et al. Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fluid microbead-based assay. J Clin Microbiol 2007; 45 (9): 2965–70.
  • Brunstein JD, Cline CL, McKinney S, Thomas E. Evidence from multiplex molecular assays for complex multipathogen interactions in acute respiratory infections. J Clin Microbiol 2008; 46 (1): 97–102.
  • Raymond F, Carbonneau J, Boucher N et al. Comparison of automated microarray detection with real-time PCR assays for detection of respiratory viruses in specimens obtained from children. J Clin Microbiol 2009; 47 (3): 743–50.
  • Beck ET, Jurgens LA, Kehl SC et al. Development of a rapid automated influenza A, influenza B, and respiratory syncytial virus A/B multiplex real-time RT-PCR assay and its use during the 2009 H1N1 swine-origin influenza virus epidemic in Milwaukee, Wisconsin. J Mol Diagn 2010; 12 (1): 74–81.
  • Pabbaraju K, Tokaryk KL, Wong S, Fox JD. Comparison of the Luminex xTAG respiratory viral panel with in-house nucleic acid amplification tests for diagnosis of respiratory virus infections. J Clin Microbiol 2008; 46 (9): 3056–62.
  • Bellau-Pujol S, Vabret A, Legrand L et al. Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses. J Virol Methods 2005; 126 (1–2): 53–63.
  • Poddar SK. Influenza virus types and subtypes detection by single step single tube multiplex reverse transcription- polymerase chain reaction (RT-PCR) and agarose gel electrophoresis. J Virol Methods 2002; 99 (1–2): 63–70.
  • Karrer EE, Lincoln JE, Hogenhout S et al. In situ isolation of mRNA from individual plant cells: creation of cell-specific cDNA libraries. Proc Natl Acad Sci USA 1995; 92 (9): 3814–8.
  • Schloss L, van Loon AM, Cinque P et al. An international external quality assessment of nucleic acid amplification of herpes simplex virus. J Clin Virol 2003; 28 (2): 175–85.
  • Kabir MS, Clements MO, Kimmitt PT. RT-Bst: an integrated approach for reverse transcription and enrichment of cDNA from viral RNA. Br J Biomed Sci 2015; 72 (1): 1–6.
  • Yoda T, Suzuki Y, Yamazaki K et al. Evaluation and application of reverse transcription loop-mediated isothermal amplification for detection of noroviruses. J Med Virol 2007; 79 (3): 326–34. Erratum in J Med Virol 2007; 79 (6): 863.
  • Fukuda S, Takao S, Kuwayama M, Shimazu Y, Miyazaki K. Rapid detection of norovirus from fecal specimens by real-time reverse transcription-loop-mediated isothermal amplification assay. J Clin Microbiol 2006; 44 (4): 1376–81.
  • Raengsakulrach B, Nisalak A, Maneekarn N et al. Comparison of four reverse transcription-polymerase chain reaction procedures for the detection of dengue virus in clinical specimens. J Virol Methods 2002; 105 (2): 219–32.
  • Dreier J, Störmer M, Kleesiek K. Use of bacteriophage MS2 as an internal control in viral reverse transcription-PCR assays. J Clin Microbiol 2005; 43 (9): 4551–7.
  • Bustin SA, Mueller R. Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis. Clin Sci (Lond) 2005; 109 (4): 365–79.

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