Transport of antiviral 3'-deoxy-nucleoside drugs by recombinant human and rat equilibrative, nitrobenzylthioinosine (NBMPR)-insensitive (ENT2) nucleoside transporter proteins produced in Xenopus oocytes

References

• Agbanyo, F. R., Cass, C. E. and Paterson, A. R. P., 1988, External location of sites on pig erythrocyte membranes that bind nitrobenzylthioinosine. Molecular Pharmacology, 33, 332 ± 337.
   PubMed Web of Science ®Google Scholar
• Baldwin, S. A., Mackey, J. R., Cass, C. E. and Young, J. D., 1999,Nucleoside transporters: molecular biology and implications for therapeutic development. Molecular Medicine Today, 5, 216 ± 224.
   PubMedGoogle Scholar
• Cass, C. E., 1995, Nucleoside transport. In Drug Transport in Antimicrobia l and Anticancer Chemotherapy, N. H. Georgopapa- dakou, ed. (New York: Marcel Dekker), pp. 404 ± 451.
   Google Scholar
• Chan, T. C. K., Boon, G. D., Shaffer, L. and Redmond, R., 1982, Antiviral nucleoside toxicity in canine bone marrow progenitor cells and its relationship to drug permeation. European Journal of Haematology, 49, 71 ± 76.
   Google Scholar
• Chan, T. C. K., Boon, G. D., Shaffer, L., Redmond, R. and Pennington, K. L., 1993, Permeation and metabolis m of anti-HIV and endogenous nucleosdies in human immune effector cells. Biochemical Pharmacology, 46, 273 ± 278.
   PubMed Web of Science ®Google Scholar
• Che, M., Ortiz, D. F. and Arias, I. M., 1995, Primary structur e and functional expression of a cDNA encoding the bile canalicular, purine-specific Na+-nucleoside cotransporter. Journal of Biological Chemistry, 270, 3596 ± 13599.
   Google Scholar
• Crawford, C. R., Patel, D. H., Naeve, C. and Belt, J. A., 1998, Cloning of the human equilibrative, nitrobenzylmercaptopurine riboside (NBMPR)-insensitive nucleoside transporter ei by func- tional expression in a transport-deficient cell line. Journal of Biologica l Chemistry, 273, 5288 ± 5293.
   PubMed Web of Science ®Google Scholar
• Domin, B. A., Mahony, W. B. and Zimmerman, T. P., 1993, Membrane permeation mechanisms of 2¢,3¢-dideoxynucleosides. Biochemical Pharmacology, 46, 725 ± 729.
   PubMed Web of Science ®Google Scholar
• Gati, W. P., Misra, H. K., Knaus, E. E. and Wiebe, L. I., 1984, Structural modifications at the 2¢- and 3¢-positions of some pyrimidine nucleosides as determinants of their interaction with the mouse erythrocyte nucleoside transporter. Biochemical Pharmacology, 33, 3325 ± 3331.
   PubMed Web of Science ®Google Scholar
• Griffith, D. A. and Jarvis, S. M., 1996, Nucleoside and nucleobas e transport systems of mammalian cells. Biochimica et Biophysica Acta, 1286, 153 ± 181.
   PubMed Web of Science ®Google Scholar
• Griffiths, M., Beaumont, N., Yao, S. Y. M., Sundaram, M., Bouman, C. E., Davies, A., Kwong, F. Y. P., Coe, I. R., Cass, C. E ., Young,J. D. and Baldwin, S. A., 1997a, Cloning of a human nucleoside transporter implicated in the cellula r uptake of adenosine and chemotherapeutic drugs. National Medicine, 3, 89 ± 93.
   PubMed Web of Science ®Google Scholar
• Griffiths, M., Yao, S. Y. M., Abidi, F., Phillips, S. E. V., Cass, C. E., Young, J. D. and Baldwin, S. A., 1997b, Molecula r cloning and characterization of a nitrobenzylthioinosine- ins ensitive (ei) equili- brative nucleoside transporter from human placenta. Biochemistry Journal, 328, 739 ± 743.
   PubMed Web of Science ®Google Scholar
• Horton, R. M., Hunt, H. D., Ho, S. N., Pullen, J. K. and Pease, L. R., 1989, Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene, 77, 1 ± 68.
   PubMedGoogle Scholar
• Huang, Q. Q., Harvey, C. M., Paterson, A. R. P., Cass, C. E. and Young, J. D., 1993, Functional expression of Na+-dependent nucleoside transport systems of rat intestine in isolated oocytes of Xenopus laevis Demonstration that rat jejunum express es the purine-selectiv e system Ni (cif) and a second, novel system N3 having broad specificity for purine and pyrimidine nucleosides. Journal of Biological Chemistry 268, 613 ± 20619.
   PubMedGoogle Scholar
• Huang, Q. Q., Yao, S. Y. M., Ritz el, M. W. L., Paterson, A. R. P.,
   Google Scholar
• Cass, C. E. and Young, J. D., 1994, Cloning and functional expression of a complementary DNA encoding a mammalian nucleoside transport protein. Journal of Biological Chemistry 269, 7757 ± 17760.
   Google Scholar
• Jarvis, S. M., 1986, Nitrobenzylthioinosine-s ensitive nucleoside transport system: mechanism of inhibition by dipyridamole. Molecular Pharmacology 30, 658 ± 665.
   Google Scholar
• Jarvis, S. M., Janmohamed, N. S. and Young, J. D., 1983, Kinetics of nitrobenzylthioinosine binding to the human erythrocyte nucleo- side transporter. Biochemistry Journal 216, 661 ± 667.
   PubMedGoogle Scholar
• Jarvis, S. M., McBride, D. and Young, J. D., 1982, E rythrocyte nucleoside transport: asymmetrical binding of nitrobenzylthioino- sine to nucleoside permeation sites. Journal of Physiology (London) 324, 31 ± 46.
   PubMed Web of Science ®Google Scholar
• Kwong, F. Y. P., Wu, J. S. R., Fincham, H. E., Davies, A., Henderson, P. J. F., Baldwin, S. A. and Young, J. D., 1993, Enzymic cleavage as a probe of the molecular structures of mammalian equilibrativ e nucleoside transporters. Journal of Biologica l Chemistry 268, 22127 ± 22134.
   PubMedGoogle Scholar
• Osses, N., Pearson, J. D., Yudilevich, D. L. and Jarvis, S. M., 1996, Hypoxanthine enters human vascular endothelial cells (ECV 304) via the nitrobenzylthioinosine-insensitiv e equilibrative nucleoside transporter. Biochemistry Journal 317, 843 ± 848.
   Google Scholar
• Parker, M. D., Hyde, R. J., Yao, S. Y., McRobert, L., Cass, C. E., Young, J. D., McConkey, G. A. and Baldwin, S. A., 2000, Identification of a nucleoside/nucleobas e transporter from Plas- modium falciparum a novel target for anti-malarial chemotherapy. Biochemistry Journal 349, 67 ± 75.
   PubMedGoogle Scholar
• Plagemann, P. G. W. and Woffendin, C., 1989, Dideoxycytidine permeation and salvage by mouse leukemia cells and human erythrocytes. Biochemical Pharmacology, 38, 3469 ± 3475.
   PubMed Web of Science ®Google Scholar
• Ritzel, M. W. L., Ng, A. M. L., Yao, S. Y. M., Graham, K., Loewen, S.K., Smith, K. M., Ritzel, G., Mowles, D. A., Carpenter, P., Chen, X.Z., Karpinski, E., Hyde, R. J., Baldwin, S. A., Cass, C. E. and Young, J. D., 2001, Molecula r identifica tion and characterization of novel human and mous e concentrativ e Na+-nucleoside cotra n- sporter proteins (hCNT3 and mCNT3) broadly s elective for purine and pyrimidine nucleosides. Journal of Biological Chemistry, 276, 2914 ± 2927.
   PubMed Web of Science ®Google Scholar
• Ritzel, M. W. L., Yao, S. Y. M., Huang, M. Y., Elliot, J. F., Cass, C. E. and Young, J. D., 1997, Molecular cloning and functional expression of cDNAs encoding a human Na+-nucleoside cotra n- sporter (hCNT1). American Journal of Physiology, 272, C707 ± C714.
   Google Scholar
• Ritzel, M. W. L., Yao, S. Y. M., Ng, A. M. L., Mackey, J. R., Cass, C.E. and Young, J. D., 1998, Molecular cloning, functiona l expression and chromosomal localization of a cDNA encoding a human Na+ /nucleoside cotransporter (hCNT2) s elective for purine nucleosides and uridine. Molecular Membrane Biology, 15, 203 ± 211.
   PubMed Web of Science ®Google Scholar
• Shayeghi, M., Akerman, R. and Jarvis, S. M., 1999, Nucleobase transport in opossum kidney epithelia l cells and Xenopus laevis oocytes: the characterisation, structure-activi ty relationship of uracil analogues and ooyte expression studies of sodium- dependent and independent hypoxanthine uptake. Biochimica et Biophysica Acta, 1416, 109 ± 118.
   PubMed Web of Science ®Google Scholar
• Sundaram, M., Yao, S. Y. M., Ng, A. M. L., Griffiths, M., Cass, C. E.,
   Google Scholar
• Baldwin, S. A. and Young, J. D., 1998, Chimeric constructs between human and rat equilibrative nucleoside transporters (hENT1 and rE NT1) reveal hENT1 structura l domains interacting with coronary vasoactive drugs. Journal of Biological Chemistry, 273, 21519 ± 21525
   PubMedGoogle Scholar
• Ullman, B., Coons, T., Rockwell, S. and McCartan, K., 1988, Genetic analysis of 2¢,3¢-dideoxycytidine incorporation into cultured human T lymphoblasts. Journal of Biological Chemistry, 263, 12391 ± 12396.
   PubMed Web of Science ®Google Scholar
• Wang, J., Su, S. F., Dress er, M. J., Schaner, M. E., Washington, C. B. and Giacomini, K. M., 1997, Na+-dependent purine nucleoside transporter from human kidney: cloning and functional character- ization. American Journal of Physiology, 273, F1058 ± F1065.
   Google Scholar
• Ward, J. L., Sherali, A., Mo, Z. P. and Tse, C. M., 2000, Kinetic and pharmaological properties of cloned human equilibrative nucleo- side transporters, ENT1 and ENT2, stably expressed in nucleo- side transporter-deficient PK15 cells. E NT2 exhibits a low affinity for guanosine and cytidine but a high affinity for inosine. Journal of Biological Chemistry, 275, 8375 ± 8381.
   Google Scholar
• Yao, S. Y. M., Cass, C. E. and Young, J. D., 1996b, Transport of the antivira l nucleoside analogs 3¢-azido-3¢-deoxythymidine and 2¢,3¢- dideoxycytidine by a recombinant nucleoside transporter (rCNT) express ed in Xenopus la evis oocytes. Molecular Pharmacology, 50, 388 ± 393.
   PubMed Web of Science ®Google Scholar
• Yao, S. Y., Cass, C. E. and Young, J. D., 2000, The Xenopus oocyte expression system for the DNA cloning and characterisation of plasma membrane transport proteins. In Membrane Transport. A Practical Approach, S.A. Baldwin, ed. (Oxford: Oxford University Press), pp. 47 ± 78.
   Google Scholar
• Yao, S. Y. M., Ng, A. M. L., Muzyka, W. R., Griffiths, M., Cass, C. E., Baldwin, S. A. and Young, J. D., 1997, Molecular cloning and functional characterizatio n of nitrobenzylthioinosine (NBMPR)- s ensitive (es) and NBMPR-ins ensitive (ei) equilibrative nucleoside transporter proteins (rE NT1 and rE NT2) from rat tissues. Journal of Biological Chemistry, 272, 28423 ± 28430.
   PubMed Web of Science ®Google Scholar
• Yao, S. Y. M., Ng, A. M. L., Ritzel, M. W. L., Gati. W. P., Cass, C. E. and Young, J. D., 1996a, Transport of adenosine by recombinant purine- and pyrimidine-s electiv e sodium/nucleoside cotranspor- ters from rat jejunum expressed in Xenopus laevis oocytes. Molecula r Pharmacology, 50, 1529 ± 1535.
   Google Scholar
• Yao, S. Y. M., Sundaram, M., Chomey, E. G., Cass, C. E., Baldwin, S. A. and Young, J. D., 2001, Identifica tion of Cys140 in helix 4 as an exofacial cysteine residue within the substrate-translocation channel of rat equilibrative nitrobenzylthioinosine (NBMPR)- insensitive nucleoside transporter rE NT2. Biochemistry Journal, 353, 387 ± 393.
   PubMed Web of Science ®Google Scholar
• Young, J. D., Chees eman, C. I., Mackey, J. R., Cass, C. E. and Baldwin, S. A., 2000, Molecular mechanisms of nucleoside and nucleoside drug transport. In Current Topics in Membranes, Vol 50, K. E. Barrett and M. Donowitz, eds (San Diego: Academic Press), pp 329 ± 378.
   Google Scholar
• Zimmerman, T. P., Mahoney, W. B. and Prus, K. L., 1987, 3¢-Azido- 3¢-deoxythymidine, an unusual nucleoside analogue that permeates the membrane of human erythrocytes and lymphocytes by non-facilitate d diffusion. Journal of Biologica l Chemistry, 262, 5748 ± 5754.
   PubMed Web of Science ®Google Scholar