Functional expression and characterization of a purine nucleobase transporter gene from Leishmania major

References

• Al-Salabi, M. I., Wallace, L. J. M. and De Koning, H. P., 2003, A Leishmania major nucleobase transporter responsible for allopur- inol uptake is a functional homolog of the Trypanosoma brucei H2 transporter. Molecular Pharmacology, 63, 814 –820.
   PubMed Web of Science ®Google Scholar
• Arriza, J. L., Kavanaugh, M. P., Fairman, W. A., Wu, Y.-N., Murdoch, G. H., North, R. A. and Amara, S. G., 1993, Cloning and expression of a human neutral amino acid tranporter with structural similarity to the glutamate transporter gene family.
   Google Scholar
• Journal of Biological Chemistry, 268, 15329 –15332.
   Google Scholar
• Berens, R. L., Krug, E. C. and Marr, J. J., 1995. Purine and pyrimidine metabolism. In Biochemistry and Molecular Biology of Parasites, J. J. Marr and M. Mu¨ ller, eds (New York: Academic Press), pp. 89 –117.
   Google Scholar
• Carter, N. S., Barrett, M. P. and de Koning, H. P., 1999, A drug resistance determinant in Trypanosoma brucei. Trends in Micro- biology, 7, 469 –471.
   PubMed Web of Science ®Google Scholar
• Carter, N. S., Drew, M. E., Sanchez, M., Vasudevan, G., Landfear, S. M. and Ullman, B., 2000, Cloning of a novel inosine-guanosine transporter gene from Leishmania donovani by functional rescue of a transport-deficient mutant. Journal of Biological Chemistry, 275, 20935–20941.
   PubMed Web of Science ®Google Scholar
• Carter, N. S., Landfear, S. M. and Ullman, B., 2001, Nucleoside transporters of parasitic protozoa. Trends in Parasitology, 17, 142 –145.
   PubMed Web of Science ®Google Scholar
• Cheng, Y. and Prusoff, W. H., 1973, Relationship between the inhibition constant K1 and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction. Biochemical Pharmacology, 22, 3099–3108.
   PubMed Web of Science ®Google Scholar
• Crawford, C. R., Patel, D. H., Naeve, C. and Belt, J. A., 1998, Cloning of the human equilibrative, nitrobenzylmercaptopurine riboside (NBMPR)-insensitive nucleoside transporter ei by func- tional expression in a transport-deficient cell line. Journal of Biological Chemistry, 273, 5288–5293.
   PubMed Web of Science ®Google Scholar
• de Koning, H. P. and Diallinas, G., 2000, Nucleobase transporters. Molecular Membrane Biology, 75, 75 -94.
   Google Scholar
• de Koning, H. P. and Jarvis, S. M., 1997, Hypoxanthine uptake through a purine-selective nucleobase transporter in Trypano- soma brucei brucei procyclic cells is driven by protomotive force. European Journal of Biochemistry, 247, 1102 -1110.
   PubMedGoogle Scholar
• de Koning, H. P. and Jarvis, S. M., 1997, Purine nucleobase transport in bloodstream forms of Trypanosoma brucei brucei is mediated by two novel transporters. Molecular Biochemistry and Parasitology, 89, 245 –258.
   PubMed Web of Science ®Google Scholar
• de Koning, H. P. and Jarvis, S. M., 1998, A highly selective, high- affinity transporter for uracil in Trypanosoma brucei brucei: evidence for proton-dependent transport. Biochemical Cell Biol- ogy, 76, 853 –858.
   PubMedGoogle Scholar
• Griffiths, M., Beaumont, N., Yao, S. Y. M., Sundaram, M., Boumah, E., Davies, A., Kwong, F. Y. P., Coe, I., Cass, C. E., Young, J.C. and Baldwin, S. A., 1997, Cloning of a human nucleoside transporter implicated in the cellular uptake of adenosine and chemotherapeutic drugs. Nature Medicine, 3, 89 –93.
   PubMed Web of Science ®Google Scholar
• Hansen, B. D., Perez-Arbelo, J., Wlakony, J. F. and Hendricks, L. D., 1982, The specificity of purine base and nucleoside uptake in promastigotes of Leishmania braziliensis panamensis. Parasitol- ogy, 85, 271 –282.
   PubMed Web of Science ®Google Scholar
• Hwang, H.-Y. and Ullman, B., 1997, Genetic analysis of purine metabolism in Leishmania donovani. Journal of Biological Chem- istry, 272, 19488–19496.
   PubMed Web of Science ®Google Scholar
• Hyde, R. J., Cass, C. E., Young, J. D. and Baldwin, S. A., 2001, The ENT family of eukaryote nucleoside and nucleobase transporters: recent advances in the investigation of structure/function relation- ships and the identification of novel isoforms. Molecular Mem- brane Biology, 18, 53 -63.
   Google Scholar
• Kerby, D. R. and Detke, S., 1993, Reduced purine accumulation is encoded on an amplified DNA in Leishmania mexicana amazo- nensis resistant to toxic nucleosides. Molecular Biochemistry and Parasitology, 60, 171 –185.
   PubMed Web of Science ®Google Scholar
• Kozak, M., 1999, Initiation of translation in prokaryotes and eukar- yotes. Gene, 234, 187 –208.
   PubMed Web of Science ®Google Scholar
• Langford, C. K., Little, B. M., Kavanaugh, M. P. and Landfear, S. M., 1994, Functional expression of two glucose transporter isoforms from the parasitic protozoan Leishmania enriettii. Journal of Biological Chemistry, 269, 17939 –17943.
   Google Scholar
• Marovu, E., Seebeck, T., Enyaru, J. C. K. and Kaminsky, R., 2001, Drug resistance in Trypanosoma brucei spp., the causative agents of sleeping sickness in man and nagana in cattle. Microbes Infection, 3, 763 –770.
   PubMedGoogle Scholar
• Marr, J. J., 1991, Purine analogs as chemotherapeutic agents in leishmaniasis and American trypanosomiasis. Journal of Labora- tory Clinical Medicine, 118, 111 –119.
   PubMed Web of Science ®Google Scholar
• Martinez, S. and Marr, J. J., 1992, Allopurinol in the treatment of American cutaneous leishmaniasis. New England Journal of Medicine, 326, 741 –744.
   PubMed Web of Science ®Google Scholar
• Ma¨ser, P., Su¨ tterlin, C., Kralli, A. and Kaminsky, R., 1999, A nucleoside transporter from Trypanosoma brucei involved in drug resistance. Science, 285, 242 –244.
   Google Scholar
• Parker, M. D., Hyde, R. J., Yao, S. Y. M., McRobert, L., Cass, C. E., Young, J. D., McConkey, G. A. and Baldwin, S. A., 2000, Identification of a nucleoside/nucleobase transporter from Plas- modium falciparum, a novel target for anti-malarial chemother- apy. Biochemistry Journal, 349, 67 –75.
   PubMedGoogle Scholar
• Sambrook, J., Fritsch, E. F. and Maniatis, T., 1989, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory).
   Google Scholar
• Sanchez, M. A., Tryon, R., Green, J., Boor, J. and Landfear, S. M., 2002a, Six related nucleoside/nucleobase transporters from Trypanosoma brucei exhibit distinct biochemical functions. Jour- nal of Biological Chemistry, 277, 21499–21504.
   PubMedGoogle Scholar
• Sanchez, M. A., Tryon, R., Vasudevan, G. and Landfear, S. M., 2002b, Nucleobase transport in kinetoplastida is mediated by ENT family members. In Molecular Parasitology Meeting XIII (Woods Hole, MA: Marine Biological Laboratory) S. Hajduk, D. Roos and D. Smith, eds., p. 265A.
   Google Scholar
• Sanchez, M. A., Ullman, B., Landfear, S. M. and Carter, N. S., 1999, Cloning and functional expression of a gene encoding a P1 type nucleoside transporter from Trypanosoma brucei. Journal of Biological Chemistry, 274, 30244–30249.
   PubMedGoogle Scholar
• Sundaram, M., Yao, S. Y. M., Ingram, J. C., Berry, Z. A., Abidi, F., Cass, C. E., Baldwin, S. A. and Young, J. D., 2001, Topology of a human equilibrative, nitrobenzylthioinosine (NBMPR)-sensitive nucleoside transporter (hENT1) implicated in the cellular uptake of adenosine and anti-cancer drugs. Journal of Biological Chem- istry, 276, 45270–45275.
   PubMed Web of Science ®Google Scholar
• Van Winkle, L. J., 1999, Biomembrane Transport (San Diego: Academic Press). Vasudevan, G., Carter, N. S., Drew, M. E., Beverley, S. M., Sanchez, M. A., Seyfang, A., Ullman, B. and Landfear, S. M., 1998, Cloning of Leishmania nucleoside transporter genes by rescue of a transport-deficient mutant. Proceedings of the National Academy of Sciences (USA), 95, 9873–9878.
   Google Scholar
• Vasudevan, G., Ullman, B. and Landfear, S. M., 2001, Point mutations in a nucleoside transporter gene from Leishmania donovani confer drug resistance and alter substrate selectivity. Proceedings of the National Academy of Sciences (USA), 98, 6092–6097.
   PubMed Web of Science ®Google Scholar
• Yao, S. Y. M., Ng, A. M. L., Vickers, M. F., Sundaram, M., Cass, C. E., Baldwin, S. A. and Young, J. D., 2002, Functional and molecular characterization of nucleobase transport by recombi- nant human and rat equilibrative nucleoside transporters 1 and 2. Journal of Biological Chemistry, 277, 24938–24948.
   PubMed Web of Science ®Google Scholar