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Original Paper

Plasma membrane microdomains: Organization, function and trafficking (Review)

&
Pages 193-205 | Received 24 Feb 2004, Published online: 09 Jul 2009

References

  • Ahmed, S. N., Brown, D. A. and London, E., 1997, On the origin of sphingolipid-cholesterol rich detergent-insoluble domains in cell membranes: physiological concentrations of cholesterol and sphingolipid induce formation of a detergent-insoluble, liquid- ordered lipid phase in model membranes. Biochemistry, 36, 10944–10953.
  • Baron, W., Decker, L., Colognato, H. andffrench-Constant, C., 2003, Regulation of integrin growth factor interactions in oligodendro- cytes by lipidraft microdomains. Curr. Biol., 13, 151 –155.
  • Bist, A., Fielding, P. E. and Fielding, C. J., 1997, Two sterol regulatory element-like sequences mediate up-regulation of caveolin gene transcription in response to low density lipoprotein free cholesterol. Proc. Natl Acad. Sci. USA, 94, 10693–10698.
  • Brown, D. A. andLondon, E., 1998, Functions of lipidrafts in biological membranes. Annu. Rev. Cell Dev. Biol., 14, 111 –136. Brown, D. A. andRose, J. K., 1992, Sorting of GPI-anchored proteins to glycolipids-enriched membrane subdomains duringtransport to the apical cell surface. Cell, 68, 533 –544.
  • Bucci, M., Gratton, J. P., Rudic, R. D., Acevedo, L., Roviezzo, F., Cirino, G. andSessa, W. C., 2000, In vivo delivery of the
  • caveolin-1 scaffolding domain inhibits nitric oxide synthesis and reduces inflammation. Nat. Med., 6, 1362 –1367.
  • Bunnel, S. C., Hong, D. I., Kardon, J. R., Yamazaki, T., McGlade, C. J., Barr, V. A. andSamelson, L. E., 2002, T cell receptor ligation induces the formation of dynamically regulated signaling assem-
  • blies. J. Cell Biol., 158, 1263 –1275.
  • Dietrich, C., Bagatolli, L. A., Volovyk, Z. N., Thompson, N. L., Levi, M., Jacobson, K. andGratton, E., 2001a, Lipidrafts reconstituted in model membranes. Biophys. J., 80, 1417 -1428.
  • Dietrich, C., Volovyk, Z. N., Levi, M., Thompson, N. L. andJacobson, K., 2001b, Partitioning of Thy-1, GM1, andcross-linked phospho-
  • lipidanalogs into lipidrafts reconstituted in supported bilayer model membranes. Proc. Natl Acad. Sci. USA, 98, 10642 – 10647.
  • Dietrich, C., Yang, B., Fujiwara, T., Kusumi, A. andJacobson, K., 2002, Relationship of lipidrafts to transient confinement zones detected by single particle tracking. Biophys. J., 82, 274 –284.
  • Drab, M., Verkade, P., Elger, M., Kasper, M., Lohn, M., Lauterbach, B., Menne, J., Lindschau, C., Mende, F., Luft, F. C., Schedl, A., Haller, H. andKurzchalia, T. V., 2001, Loss of caveolae, vascular dysfunction, and pulmonary defects in caveolin-1 gene-disrupted
  • mice. Science, 293, 2449 –2552.
  • Edidin, M., 2003, The state of lipid rafts: from model membranes to cells. Annu. Rev. Biophys. Biomol. Struct., 32, 257 –283.
  • Edidin, M., Zuniga, M. C. and Sheetz, M. P., 1994, Truncation mutants define and locate cytoplasmic barriers to lateral mobility
  • of membrane glycoproteins. Proc. Natl Acad. Sci. USA, 91, 3378 –3382.
  • Field, K. A., Holowka, D. and Baird, B., 1995, Fc epsilon RI- medieated recruitment of p53/56lyn to detergent resistant mem-
  • brane domains accompanies cellualar signalling. Proc. Natl Acad. Sci. USA, 92, 9201 –9205.
  • Fielding, C. J., Bist, A. and Fielding, P. E., 1999, Intracellular cholesterol transport in synchronizedhuman skin fibroblasts. Biochemistry, 38, 2506 -2513.
  • Fleming, I. andBusse, R., 2003, Molecular mechanisms involvedin the regulation of the endothelial nitric oxide synthase. Am. J. Physiol. Regul. Integr. Comp. Physiol., 284, R1 -R12.
  • Foster, L. J., De Hoog, C. L. andMann, M., 2003, Unbiased quantitative proteomics of lipidrafts reveals high specificity for signaling factors. Proc. Natl Acad. Sci. USA, 100, 5813 -5818.
  • Fra, A. M., Williamson, E., Simons, K. andParton, R. G., 1995, De novo formation of caveolae in lymphocytes by expression of VIP21-caveolin. Proc. Natl Acad. Sci. USA, 92, 8655 -8659.
  • Fra, A. M., Pasqualetto, E., Mancini, M. andSitia, R., 2000, Genomic organization andtranscriptional analysis of the human genes coding for caveolin-1 and caveolin-2. Gene, 243, 75 –83.
  • Fridriksson, E. K., Shipkova, P. A., Sheets, E. D., Holowka, D., Baird,
  • B. andMcLafferty, F. W., 1999, Quantitative analysis of phos- pholipids in functionally important membrane domains from RBL- 2H3 mast cells using tandem high-resolution mass spectrometry. Biochemistry, 38, 8056 -8063.
  • Friedrichson, T. and Kurzchalia, T. V., 1998, Microdomains of GPI- anchoredproteins in living cells revealed by crosslinking. Nature, 394, 802 –805.
  • Fujiwara, T., Ritchie, K., Murakoshi, H., Jacobson, K. andKusumi, A., 2002, Phospholipids undergo hop diffusion in compartmenta- lizedcell membrane. J. Cell Biol., 157, 1071 -1081.
  • Furuchi, T. and Anderson, R. G. W., 1998, Cholesterol depletion of caveolae causes hyperactivation of extracellular signal-related kinase. J. Biol. Chem., 274, 30636–30643.
  • Galbiati, F., 1998, Targeted downregulation of caveolin-1 is sufficient to drive cell transformation and hyperactivate the p42/44MAP kinase cascade. EMBO J., 17, 6633 -6648.
  • Galbiati, F., Engelman, J. A., Volonte, D., Zhang, X. L., Minetti, C., Li, M., Hou, H., Kneitz, B., Edelmann, W. and Lisanti, M. P., 2001, Caveolin-3 null mice show a loss of caveolae, changes in the
  • microdomain distribution of the dystrophin-glycoprotein complex, andt-tubule abnormalities. J. Biol. Chem., 276, 21425 –21433.
  • Garcia-Cardena, G., Martasek, P., Masters, B. S., Skidd, P. M., Couet, J., Li, S., Lisanti, M. P. andSessa, W. C., 1997, Dissecting the interaction between nitric oxide synthase (NOS) and caveolin. Functional significance of the non caveolin binding domain in
  • vivo. J. Biol. Chem., 272, 25437 –25440.
  • Gaus, K., Gratton, E., Kable, E. P. W., Jones, A. S., Gelissen, I., Kritharides, L. and Jessup, W., 2003, Visualizing lipid structure andraft domains in living cells with two-photon microscopy. Proc.
  • Natl Acad. Sci. USA, 100, 15554 –15559.
  • Germain, R. N., 2001, The T cell receptor for antigen: signaling and ligand discrimination. J. Biol. Chem., 276, 35223–35226.
  • Hagiwara, Y., Sasaoka, T., Araishi, K., Imamura, M., Yorifuji, H., Nonaka, I., Ozawa, E. andKikuchi, T., 2000, Caveolin-3 deficiency causes muscle degeneration in mice. Hum. Mol.Genet., 9, 3047 -3054.
  • Harder, T. and Kuhn, M., 2000, Selective accumulation of raft- associatedmembrane protein LAT in T cell receptor signaling assemblies. J. Cell Biol., 151, 199 –208.
  • Heerklotz, H., 2002, Triton promotes domains formation in lipid raft mixtures. Biophys. J., 83, 2693 -2701.
  • Holmgren, J., 1973, Comparison of the tissue receptors for Vibrio cholerae andEscherichia coli enterotoxins by means of ganglio- sides and natural cholera toxoid. Infect. Immun., 8, 851 –859.
  • Huang, C., Hepler, J. R., Chen, L. T., Gilman, A. G., Anderson, R. G. andMumby, S. M., 1997, Organization of G proteins andadenylyl
  • cyclase at the plasma membrane. Mol. Biol. Cell, 8, 2365 –2378. Ipsen, J. H., Karlstro˜ m, G., Mouritsen, O. G., Wennerstro˜ m, H. and
  • Zuckermann, M. J., 1987, Phase equilibrium in the phosphatidyl- choline-cholesterol system. Biochim. Biophys. Acta, 905, 162 – 172.
  • Ishikawa, H., 1968, Formation of elaborate networks of T-system tubules in culturedskeletal muscle with special reference to the T- system formation. J. Cell Biol., 38, 51 –66.
  • Isshiki, M. and Anderson, R. G., 1999, Calcium signal transduction from caveolae. Cell Calcium, 26, 201 –208.
  • Janes, P. W., Ley, S. C. andMagee, A. I., 1999, Aggregation of lipid rafts accompanies signaling via the T cell antigen receptor. J. Cell Biol., 147, 447 –461.
  • Kabouridis, P. S., Magee, A. I. and Ley, S. C., 1997, S-acylation of LCK protein tyrosine kinase is essential for its signalling function in T lymphocytes. EMBO J., 16, 4983 -4998.
  • Kenworthy, A. K. and Edidin, M., 1998, Distribution of a glycopho- sphatidylinositol-anchored protein at the apical surface of MDCK cell examinedat a reolution of B100A° using imaging fluores-
  • cence resonance energy transfer. J. Cell Biol., 142, 69 –84.
  • Kenworthy, A. K., Petranova, N. and Edidin, M., 2000, High- resolution FRET microscopy of cholera toxin B-subunit and GPI-anchoredproteins in cell plasma membranes. Mol. Biol. Cell, 11, 1645 -1655.
  • Kurzchalia, T. V., Dupree, P., Parton, R. G., Kellner, R., Virta, H., Lehnert, M. andSimons, K., 1992, VIP21, a 21-kD membrane
  • protein is an integral component of trans-Golgi-network-derived transport vesicles. J. Cell Biol., 118, 1003 –1014.
  • Kusumi, A., Sako, Y. andYamamoto, M., 1993, Confinedlateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differ-
  • entiation in culturedepithelial cells. Biophys. J., 65, 2021 –2040. Kwik, J., Boyle, S., Fooksman, D., Margolis, L., Sheetz, M. P. and
  • Edidin, M., 2003, Membrane cholesterol, lateral mobility, and the phosphatidylinositol 4,5-bisphosphate-dependent organization of cell actin. Proc. Natl Acad. Sci. USA, 100, 13964–13969.
  • Lahtinen, U., Honsho, M., Parton, R. G., Simons, K. andVerkade, P., 2003, Involvement of caveolin-2 in caveolar biogenesis in MDCK cells. FEBS Lett., 538, 85 –88.
  • Lang, T., Bruns, D., Wenzel, D., Riedel, D., Holroyd, P., Thiele, C. andJahn, R., 2001, SNAREs are concentratedin cholesterol- dependent clusters that define docking and fusion sites for exocytosis. EMBO J., 20, 2202 -2213.
  • Le, P. U., Guay, G., Altschuler, Y. andNabi, I. R., 2002, Caveolin-1 is a negative regulator of caveolae-mediated endocytosis to the endoplasmic reticulum. J. Biol. Chem., 277, 3371 -3379.
  • Lisanti, M. P., Scherer, P. E., Tang, Z. L. andSargiacomo, M., 1994, Caveolae, caveolin andcaveolin-rich membrane domains: A signalling hypothesis. Trends Cell Biol., 4, 231 –235.
  • Liu, P., Ying, Y., Ko, Y. G. andAnderson, R. G., 1996, Localization of platelet-derived growth factor-stimulated phosphorylation cas- cade to caveolae. J. Biol. Chem., 271, 10299–10303.
  • London, E., 2002, Insights into lipid raft structure and formation from experiments in model membranes. Curr. Opin. Struct. Biol., 12, 480 –486.
  • McCabe, J. B. andBerthiaume, L. G., 2001, N-terminal protein acylation confers localization to cholesterol, sphingolipid-enriched
  • membranes but not to lipidrafts/caveolae. Mol. Biol. Cell, 12, 3601 –3617.
  • Madore, N., Smith, K. L., Graham, C. H., Jen, A., Brady, K., Hall, S. andMorris, R., 1999, Functionally different GPI proteins are organized in different domains on the neuronal surface. EMBO J., 18, 6917 –6926.
  • Melkonian, K. A., Ostermeyer, A. G., Chen, J. Z., Roth, M. G. and Brown, D. A., 1999, Role of lipidmodifications in targeting proteins to detergent-resistant membrane rafts. Many proteins
  • are acylated, while few are prenylated. J. Biol. Chem., 274, 3910 –3917.
  • Moffet, S., Brown, D. A. and Linder, M. E., 2000, Lipid-dependent targeting of G proteins into rafts. J. Biol. Chem., 275, 2191 –2198. Monier, S., Parton, R. G., Vogel, F., Behlke, J., Henske, A. and
  • Kurzchalia, T. V., 1995, VIP21-caveolin, a membrane protein constituent of the caveolar coat, oligomerizes in vivo and in vitro. Mol. <i>Biol. Cell, 6, 911 –927.
  • Montesano, R., Roth, J., Robert, A. andOrci, L., 1982, Non-coated membrane invaginations are involvedin binding andinternaliza- tion of cholera andtetanus toxins. Nature, 296, 651 –653.
  • Montixi, C., 1998, Engagement of T-cell receptor triggers its recruitment to low-density detergent-insoluble membrane do- mains. EMBO J., 17, 5334 -5348.
  • Munro, S., 2003, Lipidrafts: elusive or illusive? Cell, 115, 377 –388. Murata, M., Peranen, J., Schreiner, R., Wieland, F., Kurzchalia, T. V.
  • andSimons, K., 1995, VIP21/caveolin is a cholesterol binding protein. Proc. Natl Acad. Sci. USA, 92, 10339–10343.
  • Murray, D., Ben-Tal, N., Honig, B. andMcLaughlin, S., 1997, Electrostatic interaction of myristoylatedproteins with membra-
  • nes:simplephysics, complicatedbiology. Structure, 5, 985 -989. Murray, D., Arbuzova, A., Hangyas-Mihalyne, G., Gambhir, A., Ben-
  • Tal, N., Honig, B. andMcLaughlin, S., 1999, Electrostatic properties of membranes containing acidic lipids and adsorbed basic peptides: theory and experiment. Biophys. J., 77, 3176- 3188.
  • Nabi, I. R. and Le, P. U., 2003, Caveolae/raft-dependent endocy- tosis. J. Cell Biol., 161, 673 –677.
  • Nichols, B. J., 2002, A distinct class of endosome mediates clathrin- independent endocytosis to the Golgi complex. Nat. Cell Biol., 4, 374 –378.
  • Nichols, B. J., 2003, Caveosomes andendocytosis of lipidrafts. J. Cell Sci., 116, 4707 -4714.
  • Nichols, B. J., Kenworthy, A. K., Polischuk, R. S., Lodge, R., Roberts, T. H., Hirshberg, K., Phair, R. D. andLippincott- Schwartz, J., 2001, Rapidcycling of lipidraft markers betweenthe cell surface andgolgi complex. J. Cell Biol., 153, 529 –542.
  • Niv, H., Gutman, O., Kloog, Y. andHenis, Y., 2002, ActivatedK-ras and H-ras display different interactions with saturable nonraft sites at the surface of live cells. J. Cell Biol., 157, 865 –872.
  • Nomura, R., Inuo, C., Takahashi, Y., Asano, T. andFujimoto, T., 1997, Two-dimensional distribution of Gi2a in the plasma mem- brane: a critical evaluation by immunocytochemistry. FEBS Lett. ,415, 139 –144.
  • Oh, P., McInitosh, D. P. andSchnitzer, J. E., 1998, Dynamin at the neck of caveolae mediates their budding to form transport vesicles by GTP-driven fission from the plasma membrane of endothelium. J. Cell Biol., 141, 101 –114.
  • Okamoto, T., Schlegel, A., Scherer, P. E. andLisanti, M. P., 1998, Caveolins, a family of scaffolding proteins for organizing ‘pre- assembledsignaling complexes’ at the plasma membrane. J. Biol. Chem., 273, 5419 -5422.
  • Orlandi, P. A. and Fishman, P. H., 1998, Filipin-dependent inhibition of cholera toxin: evidence for toxin internalization and activation through caveolae-like domains. J. Cell Biol., 141, 905 –915.
  • Palade, G. E., 1953, Fine structure of blood capillaries. J. Appl.Phys., 24, 1424.
  • Park, D. S., Cohen, A. W., Frank, P. G., Razani, B., Lee, H., Williams, T. M., Chandra, M., Shirani, J., De Souza, A. P., Tang, B., Jelicks, L. A., Factor, S. M., Weiss, L. M., Tanowitz, H. B. and Lisanti, M. P., 2003, Caveolin null (-/-) mice show dramatic reductions in life span. Biochemistry, 42, 15124 –15131.Parton, R. G., 1996, Caveolae andcaveolins. Curr. Opin. Cell Biol., 8, 542 -548.
  • Parton, R. G. andRichards, A. A., 2003, Lipid rafts andcaveolae as portals for endocytosis: new insights and common mechanisms. Traffic, 4, 724 –738.
  • Parton, R. G., Joggerst, B. andSimons, K., 1994, Regulated internalization of caveolae. J. Cell Biol., 127, 1199 -1215.
  • Pelkmans, L., Kartenbeck, J. andHelenius, A., 2001, Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular- transport pathway to the ER. Nat. Cell Biol., 3, 473 –483.
  • Pelkmans, L., Puntener, D. andHelenius, A., 2002, Local actin polymerization and dynamin recruitment in SV40-induced inter- nalization of caveolae. Science, 296, 535 –539.
  • Pike, L. J. andCasey, L., 1996, Localization andturnover of phosphatidylinositol 4,5-bisphosphate in caveolin-enriched mem- brane domains. J. Biol. Chem., 271, 26453–26456.
  • Pike, L. J. and Miller, J. M., 1998, Cholesterol depletion delocalizes phosphatidylinositol bisphosphate and inhibits hormone-stimu- latedphosphatidylinositol turnover. J. Biol. Chem., 273, 22298 -22304.
  • Pike, L. J., Han, X., Chung, K. N. andGross, R. W., 2002, Lipidrafts are enrichedin arachidonic acidand plasmenylethanolamine and
  • their composition is independent of caveolin-1 expression: a quantitative electrospray ionization/mass spectrometric analysis. Biochemistry, 41, 2075 -2088.
  • Pol, A., Luetterforst, R., Lindsay, M. R., Heino, S., Ikonen, E. and Parton, R. G., 2001, A caveolin dominant-negative mutant associates with lipidbodies on the caveolin-cycling pathway and
  • induces intracellular cholesterol imbalance. J. Cell Biol., 152, 1057 –1070.
  • Pralle, A., Keller, P., Florin, E. L., Simons, K. andHorber, J. K., 2000, Sphingolipid-cholesterol rafts diffuse as small entities in the plasma membrane of mammalian cells. J. Cell Biol., 148, 997 -
  • Prior, I. A., Harding, A., Yan, J., Sluimer, J., Parton, R. G. and Hancock, J. F., 2001, GTP-dependent segregation of H-ras from lipidrafts is required for biological activity. Nat. Cell Biol., 3, 368 -
  • Prior, I. A., Muncke, C., Parton, R. G. andHancock, J. F., 2003, Direct visualization of Ras proteins in spatially distinct cell surface microdomains. J. Cell Biol., 160, 165 –170.
  • Razani, B., Engelman, J. A., Wang, X. B., Schubert, W., Zhang, X. L., Marks, C. B., Macaluso, F., Russel, R. G., Li, M., Pestell, R.
  • G., Di Vizio, D., Hou, H., Kneitz, B., Lagaud, G., Christ, G. J., Edelmann, W. and Lisanti, M. P., 2001, Caveolin-1 null mice are viable but show evidence of hyperproliferative and vascular abnormalities. J. Biol. Chem., 276, 38121 –38138.
  • Razani, B., Combs, T. P., Wang, X. B., Frank, P. G., Park, D. S.,
  • Russel, R. G., Li, M., Tang, B., Jelicks, L. A., Scherer, P. E. and Lisanti, M. P., 2002a, Caveolin-1 deficient mice are lean, resistant to diet-induced obesity, and show hypertriglyceridemia with
  • adipocyte abnormalities. J. Biol. Chem., 277, 8635 –8647.
  • Razani, B., Wang, X. B., Engelman, J. A., Battista, M., Lagaud, G., Zhang, X. L., Kneitz, B., Hou, H., Christ, G. J., Edelmann, W. and Lisanti, M. P., 2002b, Caveolin-2-deficient mice show evidence of severe pulmonary dysfunction without disruption of caveolae. Mol. Cell Biol., 22, 2329 -2344.
  • Rietveld, A., Neutz, S., Simons, K. and Eaton, S., 1999, Association of sterol- andglycophosphatidylinositol-linked proteins with Dro- sophila raft microdomains. J. Biol. Chem., 274, 12049–12054.
  • Ringerike, T., Blystad, F. D., Levy, F. O., Madshus, I. H. and Stang, E., 2002, Cholesterol is important in control of EGF-receptor
  • kinase activity but EGF receptors are not concentratedin caveolae. J. Cell Sci., 115, 1331 –1340.
  • Ritchie, K., Iino, R., Fujiwara, T., Murase, K. andKusumi, A., 2003, The fence andpicket structure of the plasma membrane of live cells as revealedby single molecule techniques. Mol. Membr.
  • Biol., 20, 13 –18.
  • Rodal, S. K., Skretting, G., Garred, Ø., Vilhardt, F., van Deurs, B. andSandvig, K., 1999, Extraction of cholesterol with methyl-b- cyclodextrin perturbs formation of clathrin-coated endocytic ve- sicles. Mol. Biol. Cell, 10, 961 –974.
  • Rothberg, K. G., Ying, Y. S., Kamen, B. A. andAnderson, R. G., 1990, Cholesterol controls the clustering of the glycophospholipid- anchoredmembrane receptor for 5-methyltetrahydrofolate. J. Cell
  • Biol., 111, 2931 –2938.
  • Rothberg, K. G., Heuser, J. E., Donzell, W. C., Ying, Y. S., Glenney,
  • J. R. andAnderson, R. G. W., 1992, Caveolin, a protein component of caveolae membrane coats. Cell, 68, 673 –682.
  • Roy, S., Luetterforst, R., Harding, A., Apolloni, A., Etheridge, M., Stang, E., Rolls, B., Hancock, J. F. andParton, R. G., 1999, Dominant-negative caveolin inhibits H-ras function by disrupting cholesterol-rich plasma membrane domains. Nat. Cell Biol., 1, 98-105.
  • Sabharanjak, S., Sharma, P., Parton, R. G. andMayor, S., 2002, GPI-anchored proteins are delivered to recycling endosomes via a distinct cdc42-regulated clathrin-idependent pinocytic pathway. Dev. Cell, 2, 411 –423.
  • Sako, Y. andKusumi, A., 1994, Compartmentalized structure of the plasma-membrane for receptor movements as revealedby a
  • nanometer-level motion analysis. J. Cell Biol., 125, 1251 –1264. Samsonov, A. V., Mihalyov, I. andCohen, F. S., 2001, Character-
  • ization of cholesterol-sphingomyelin domains and their dynamics in bilayer membranes. Biophys. J., 81, 1486 –1500.
  • Sargiacomo, M., Sudol, M., Tang, Z. and Lisanti, M. P., 1993, Signal transducing molecules and glycosyl-phosphatidylinositol-linked proteins form a caveolin-rich insoluble complex in MDCK cells. J. Cell Biol., 122, 789 –807.
  • Schroeder, R., London, E. and Brown, D. A., 1994, Interactions between saturatedacyl chains confer detergent resistence on lipids and GPI-anchoredproteins: GPI-anchoredproteins in
  • liposomes andcells show similar behaviour. Proc. Natl Acad. Sci. USA, 91, 12130 –12134.
  • Schubert, W., Frank, P. G., Razani, B., Park, D. S., Chow, C. W. and Lisanti, M. P., 2001, Caveolae-deficient endothelial cells show defects in the uptake and transport of albumin in vivo. J. Biol.
  • Chem., 276, 48619 –48622.
  • Schuck, S., Honsho, M., Ekroos, K., Shevchenko, A. andSimons, K., 2003, Resistance of cell membranes to different detergents. Proc. Natl Acad. Sci. USA, 100, 5795 -5800.
  • Schutz, G. J., Kada, G., Pastushenko, V. P. and Schindler, H., 2000, Properties of lipidmicrodomains in a muscle cell membrane visualizedby single molecule microscopy. EMBO J., 19, 892 –901.
  • Sharma, D. K., Choudhary, A., Singh, R. D., Wheatley, C. L., Marks,
  • D. L. andPagano, R. E., 2003, Glycosphingolipids internalizedvia caveolar-related endocytosis rapidly merge with the clathrin pathway in early endosomes and form microdomains for recy- cling. J. Biol. Chem., 278, 7564 -7572.
  • Sharma, P., Varma, R., Sarasij, R. C., Ira, Gousset, K., Krishna- moorthy, G., Rao, M. andMayor, S., 2004, Nanoscale organiza- tion of multiple GPI-anchoredproteins in living cell membranes.
  • Cell, 116, 577 -589.
  • Shaul, P. W., Smart, E. J., Robinson, L. J., German, Z., Yuhanna, I. S., Ying, Y., Anderson, R. G. and Michel, T., 1996, Acylation targets endothelial nitric-oxide synthase to plasmalemmal caveo- lae. J. Biol. Chem., 271, 6518 -6522.
  • Sheets, E. D., Lee, G. M., Simson, K. andJacobson, K., 1997, Transient confinement of a glycosylphosphatidylinositol-anchored protein in the plasma membrane. Biochemistry, 36, 12449 -12458.
  • Sheets, E. D., Holowka, D. andBaird, B., 1999, Critical role for cholesterol in Lyn-mediated tyrosine phosphorylation of FceRI andtheir association with detergent-resistant membranes. J. Cell Biol., 145, 877 –887.
  • Shogomori, H. and Brown, D. A., 2003, Use of detergent to study membrane rafts: the good, the bad, and the ugly. Biol. Chem., 384, 1259–1263.
  • Silvius, J. R., 2003, Role of cholesterol in lipidraft formation: lessons from lipidmodel systems. Biochim. Biophys. Acta, 1610, 174 – 183.
  • Silvius, J. R., del Giudice, D. and Lafleur, M., 1996, Cholesterol at different bilayer concentrations can promote or antagonize lateral segregation of phospholipids of differing acyl chain length.
  • Biochemistry, 35, 15198 –15208.
  • Simons, K. andIkonen, E., 1997, Functional rafts in cell membranes.
  • Nature, 387, 569 -572.
  • Simons, K. andToomre, D., 2000, Lipidrafts and signal transduction.
  • Nat. Rev. Mol. Cell Biol., 1, 31 –39.
  • Simons, K. andvan Meer, G., 1988, Lipidsorting in epithelial cells.
  • Biochemistry, 27, 6197 –6202.
  • Simons, K. andWandinger-Ness, A., 1990, Polarizedsorting in epithelia. Cell, 62, 207 –210.
  • Simson, R., Sheets, E. D. andJacobson, K., 1995, Detection of temporary lateral confinement of membrane proteins using single- particle tracking analysis. Biophys. J., 69, 989 –993.
  • Simson, R., Yang, B., Moore, S. E., Doherty, P., Walsh, F. S. and Jacobson, K. A., 1998, Structural mosaicism on the submicron scale in the plasma membrane. Biophys. J., 74, 297 –308.
  • Singer, S. J. andNicholson, G. L., 1972, The fluidmosaic model of the structure of cell membranes. Science, 175, 720 –731.
  • Smart, E. J., Ying, Y., Donzell, W. C. andAnderson, R. G. W., 1996, A role for caveolin in transport of cholesterol from endoplasmic reticulum to plasma membrane. J. Biol. Chem., 271, 29427 -29435.
  • Song, S. K., Li, S., Okamoto, T., Quilliam, L. A., Sargiacomo, M. and Lisanti, M. P., 1996, Co-purification and direct interaction of Ras
  • with caveolin, an integral membrane protein of caveolae micro- domains. Detergent-free purification of caveolae microdomains. J. Biol. Chem., 271, 9690 –9697.
  • Song, K. S., Sargiacomo, M., Galbiati, F., Parenti, M. andLisanti, M. P., 1997, Targeting of a G alpha subunit (Gi1 alpha) and c-Src tyrosine kinase to caveolae membranes: clarifying the
  • role of N-myristoylation. Cell Mol. Biol. (Noisy-le-grand), 43, 293 -303.
  • Sotgia, F., Razani, B., Bonuccelli, G., Schubert, W., Battista, M., Lee, H., Capozza, F., Schubert, A. L., Minetti, C., Buckley, J. T. andLisanti, M. P., 2002, Intracellular retention of glycopho-
  • sphatidyl inositol-linked proteins in caveolin-deficient cells. Mol. Cell Biol., 22, 3905 –3926.
  • Sowa, G., Pypaert, M., Fulton, D. andSessa, W. C., 2003, The phosphorylation of caveolin-2 on serines 23 and36 modulates
  • caveolin-1-dependent caveolae formation. Proc. Natl Acad. Sci. USA, 100, 6511 –6516.
  • Stauffer, T. P. andMeyer, T., 1997, Compartmentalized IgE receptor-mediated signal transduction in living cells. J. Cell Biol., 139, 1447 -1454.
  • Subczynski, W. K. andKusumi, A., 2003, Dynamics of raft molecules in the cell andartificial membranes: approaches by pulse EPR spin labeling andsingle molecule optical microscopy. Biochim. Biophys. Acta, 1610, 231 –243.
  • Subtil, A., Gaidarov, I., Kobylarz, K., Lampson, M. A., Keen, J. H. andMcGraw, T. E., 1999, Acute cholesterol depletion inhibits clathrin-coated pit budding. Proc. Natl Acad. Sci. USA, 96, 6775 -6780.
  • Thomas, J. L., Holowka, D., Baird, B. and Webb, W. W., 1994, Large-scale co-aggregation of fluorescent lipidprobes with cell surface proteins. J. Cell Biol., 125, 795 –802.
  • Thomsen, P., Roepstorff, K., Stahlhut, M. andvan Deurs, B., 2002, Caveolae are highly immobile plasma membrane microdomains which are not involvedin constitutive endocytic trafficking. Mol.Biol. Cell, 13, 238 –250.
  • Torgersen, M. L., Skretting, G., van Deurs, B. andSandvig, K., 2001, Internalization of cholera toxin by different endocytic mechan- isms. J. Cell Sci., 114, 3737 -3747.
  • Tran, D. J., Carpentier, J. L., Sawano, F., Gorden, P. and Orci, L., 1987, Ligands internalisedthrough coatedor non-coatedinvagi- nations follow a common intracellular pathway. Proc. Natl Acad.Sci. USA, 84, 7957 -7961.
  • Trigatti, B. L., Anderson, R. G. W. and Gerber, G. E., 1999, Identification of caveolin-1 as a fatty acid binding protein. Biochem. Biophys. Res. Commun., 255, 34 –39.
  • Uittenbogaard, A., Ying, Y. S. and Smart, E. J., 1998, Characteriza- tion of a cytosolic heat-shock protein caveolin chaperone com- plex. Involvement in cholesterol trafficking. J. Biol. Chem., 275,25595 -25599.
  • van Deurs, B., Roepstorff, K., Hommelgaard, A. M. and Sandvig, K., 2003, Caveolae: anchored, multifunctional platforms in the lipid ocean. Trends Cell Biol., 13, 92 –100.
  • Varma, R. andMayor, S., 1998, GPI-anchoredproteins are organizedin submicron domains at the cell surface. Nature, 394, 798 –801.
  • Vereb, G., Szo¨ llo¨ si, J., Matko´, J., Nagy, P., Farkas, T., Vigh, L., Matyus, L., Waldmann, T. A. and Damjanovich, S., 2003, Dynamic yet structured: the cell membrane three decades after the Singer-Nicholson model. Proc. Natl Acad. Sci. USA, 100, 8053-8058.
  • Wang, T. Y. and Silvius, J. R., 2001, Cholesterol does not induce segregation of liquid-ordered domains in bilayers modeling the inner leaflet of the plasma membrane. Biophys. J., 81, 2762- 2773.
  • Wang, T. Y., Leventis, R. andSilvius, J. R., 2000, Fluorescence- based evaluation of the partitioning of lipids and lipidated peptides into liquid-ordered microdomains: a model for molecular partition-ing into ‘lipidrafts’. Biophys. J., 79, 919 –933.
  • Wang, T. Y., Leventis, R. andSilvius, J. R., 2001, Partitioning of lipidated peptide sequences into liquid-ordered lipid domains in model and biological membranes. Biochemistry, 40, 13031 - 13040.
  • Waugh, M. G., Lawson, D. andHsuann, J. J., 1999, Epidermal growth factor receptor activation is localizedwithin low-buoyant
  • density, non-caveolar membrane domains. Biochem. J., 337, 591 -597.
  • Xavier, R., Brennan, T., Li, Q., McCormack, C. andSeed, B., 1998, Membrane compartmentation is requiredfor efficient T cell activation. Immunity, 8, 723 –732.
  • Xu, X. andLondon, E., 2000, The effect of sterol structure on membrane lipid domains reveals how cholesterol can induce lipid domain formation. Biochemistry, 39, 843 –849.
  • Yamabhai, M. andAnderson, R. G. W., 2002, Secondcysteine-rich region of epidermal growth factor receptor contains targeting information for caveolae/rafts. J. Biol. Chem., 277, 24843–24846.
  • Yamada, E., 1955, The fine structure of the gall bladder epithelium of the mouse. J. Biophys. Biochem. Cytol., 1, 445 –458.
  • Zacharias, D. A., Violin, J. D., Newton, A. C. andTsien, R. Y., 2002, Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science, 296, 913 –916.
  • Zhang, W., Trible, R. P. andSamelson, L. E., 1998, LAT palmitoyla- tion: its essential role in membrane microdomain targeting and tyrosine phosphorylation during T cell activation. Immunity, 9, 239-246.

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