The conserved sequence NXX[S/T]HX[S/T]QDXXXT of the lactate/pyruvate:H⁺ symporter subfamily defines the function of the substrate translocation pathway

References

• Abramson J, Smirnova I, Kasho V, Verner G, Kaback H R, Iwata S. Structure and mechanism of the lactose permease of Escherichia coli. Science 2003; 301: 610–615
   PubMed Web of Science ®Google Scholar
• Andrade RP, Casal M. Expression of the lactate permease gene JEN1 from the yeast Saccharomyces cerevisiae. Fungal Genet Biol 2001; 32: 105–111
   PubMed Web of Science ®Google Scholar
• Andrade RP, Kotter P, Entian KD, Casal M. Multiple transcripts regulate glucose-triggered mRNA decay of the lactate transporter JEN1 from Saccharomyces cerevisiae. Biochem Biophys Res Commun 2005; 332: 254–262
   PubMedGoogle Scholar
• Ansaldi M, Lepelletier M, Mejean V. Site-specific mutagenesis by using an accurate recombinant polymerase chain reaction method. Anal Biochem 1996; 234: 110–111
   PubMedGoogle Scholar
• Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K. Current protocols in molecular biology. Wiley Interscience, New York 2002
   Google Scholar
• Balendiran GK, Molina JA, Xu Y, Torres-Martinez J, Stevens R, Focia PJ, Eakin AE, Sacchettini JC, Craig SP. Ternary complex structure of human HGPRTase, PRPP, Mg2+, and the inhibitor HPP reveals the involvement of the flexible loop in substrate binding. Protein Sci 1999; 8: 1023–1031
   PubMed Web of Science ®Google Scholar
• Biegert A, Mayer C, Remmert M, Söding J, Lupas A. The MPI Toolkit for protein sequence analysis. Nucleic Acids Res 2006; 34: W335–W339
   Google Scholar
• Casal M, Paiva S, Andrade RP, Gancedo C, Leão C. The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1. J Bacteriol 1999; 181: 2620–2623
   PubMed Web of Science ®Google Scholar
• Corpet F. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res 1988; 16: 10881–10890
   PubMed Web of Science ®Google Scholar
• Fang WG, Zhang YJ, Xiao YH, Ma JC, Yang XY, Pei Y. Isolation and characterization of a carboxylic transport protein JEN1 and its promoter from Metarhizium anisopliae. Yi Chuan Xue Bao 2003; 30: 283–288
   PubMedGoogle Scholar
• Garcia CK, Goldstein JL, Pathak RK, Anderson RGW, Brown MS. Molecular characterization of a membrane transporter for lactate, pyruvate, and other monocarboxylates – implications for the Cori Cycle. Cell 1994; 76: 865–873
   PubMed Web of Science ®Google Scholar
• Gietz RD, Woods RA. Transformation of yeast by the LiAc/SS carrier DNA/PEG method. Methods Enzymol 2002; 350: 87–96
   PubMed Web of Science ®Google Scholar
• Goldstein AL, McCusker JH. Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast 1999; 15: 1541–1553
   PubMed Web of Science ®Google Scholar
• Guex N, Peitsch MC. SWISS-MODEL and the Swiss-PdbViewer: An environment for comparative protein modeling. Electrophoresis 1997; 18: 2714–2723
   PubMed Web of Science ®Google Scholar
• Halestrap AP, Price NT. The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation. Biochem J 1999; 343: 281–299
   PubMed Web of Science ®Google Scholar
• Huang YF, Lemieux MJ, Song JM, Auer M, Wang DN. Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli. Science 2003; 301: 616–620
   PubMed Web of Science ®Google Scholar
• Koukaki M, Vlanti A, Goudela S, Pantazopoulou A, Gioule H, Tournaviti S, Diallinas G. The nucleobase-ascorbate transporter (NAT) signature motif in UapA defines the function of the purine translocation pathway. J Mol Biol 2005; 350: 499–513
   PubMedGoogle Scholar
• Krogh A, Larsson B, von Heijne G, Sonnhammer ELL. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 2001; 305: 567–580
   PubMed Web of Science ®Google Scholar
• Lodi T, Fontanesi F, Ferrero I, Donnini C. Carboxylic acids permeases in yeast: two genes in Kluyveromyces lactis. Gene 2004; 339: 111–119
   PubMedGoogle Scholar
• Ma H, Kunes S, Schatz PJ, Botstein D. Plasmid construction by homologous recombination in yeast. Gene 1987; 58: 201–216
   PubMed Web of Science ®Google Scholar
• Marger MD, Saier MHJ. A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiport. Trends Biochem Sci 1993; 18: 13–20
   PubMed Web of Science ®Google Scholar
• Paiva S, Devaux F, Barbosa S, Jacq C, Casal M. Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae. Yeast 2004; 21: 201–210
   PubMedGoogle Scholar
• Paiva S, Kruckeberg AL, Casal M. Utilization of green fluorescent protein as a marker for studying the expression and turnover of the monocarboxylate permease Jen1p of Saccharomyces cerevisiae. Biochem J 2002; 363: 737–744
   PubMedGoogle Scholar
• Pantazopoulou A, Diallinas G. The first transmembrane segment (TMS1) of UapA contains determinants necessary for expression in the plasma membrane and purine transport. Mol Membr Biol 2006; 23: 337–348
   PubMed Web of Science ®Google Scholar
• Sali A, Blundell TL. Comparative protein modelling by satisfaction of spatial restraints. J Mol Biol 1993; 234: 779–815
   PubMed Web of Science ®Google Scholar
• Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: a Laboratory Manual. Cold Spring Harbor. Cold Spring Harbor Laboratory Press, New York 1989
   Google Scholar
• Soares-Silva I, Paiva S, Kotter P, Entian K D, Casal M. The disruption of JEN1 from Candida albicans impairs the transport of lactate. Mol Membr Biol 2004; 21: 403–411
   PubMed Web of Science ®Google Scholar
• Soares-Silva I, Schuller D, Andrade RP, Baltazar F, Cássio F, Casal M. Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris. Biochem J 2003; 376: 781–787
   PubMed Web of Science ®Google Scholar
• Söding J, Biegert, Lupas AN. The HHpred interactive server for protein homology detection and structure prediction. Nucleic Acids Res 2005; 33: W244–248
   PubMed Web of Science ®Google Scholar
• Vimr ER, Troy FA. Identification of an inducible catabolic system for sialic acids (nan) in Escherichia coli. J Bacteriol 1985; 164: 845–853
   PubMed Web of Science ®Google Scholar
• Wach A, Brachat A, Alberti-Segui C, Rebischung C, Philippsen P. Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae. Yeast 1997; 13: 1065–1075
   PubMed Web of Science ®Google Scholar