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Original

Hyper- and hyporesponsive mutant forms of the Saccharomyces cerevisiae Ssy1 amino acid sensor

, &
Pages 164-176 | Received 18 Jul 2007, Published online: 09 Jul 2009

References

  • Boles E, André B. Role of transporter-like sensors in glucose and amino acid signalling in yeast. Top Curr Genet 2004; 9: 121–153
  • Holsbeeks I, Lagatie O, Van Nuland A, Van de Velde S, Thevelein JM. The eukaryotic plasma membrane as a nutrient-sensing device. Trends Biochem Sci 2004; 29: 556–563
  • Hyde R, Taylor PM, Hundal HS. Amino acid transporters: roles in amino acid sensing and signaling in animal cells. Biochem J 2003; 373: 1–18
  • Forsberg H, Ljungdahl PO. Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids. Mol Cell Biol 2001; 21: 814–826
  • Klasson H, Fink GR, Ljungdahl PO. Ssy1p and Ptr3p are plasma membrane components of a yeast system that senses extracellular amino acids. Mol Cell Biol 1999; 19: 5405–5416
  • Poulsen P, Wu B, Gaber RF, Kielland-Brandt MC. Constitutive signal transduction by mutant Ssy5p and Ptr3p components of the SPS amino acid sensor system in Saccharomyces cerevisiae. Eukaryot Cell 2005; 4: 1116–1124
  • Chang AB, Lin R, Studley WK, Tran CV, Saier MH, Jr. Phylogeny as a guide to structure and function of membrane transport proteins. Mol Membr Biol 2004; 21: 171–181
  • Nelissen B, De Wachter R, Goffeau A. Classification of all putative permeases and other membrane plurispanners of the major facilitator superfamily encoded by the complete genome of Saccharomyces cerevisiae. FEMS Microbiol Rev 1997; 21: 113–134
  • Krogh A, Larsson B, von Heijne G, Sonnhammer EL. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 2001; 305: 567–580
  • Bernard F, André B. Genetic analysis of the signalling pathway activated by external amino acids in Saccharomyces cerevisiae. Mol Microbiol 2001; 41: 489–502
  • Didion T, Regenberg B, Jørgensen MU, Kielland-Brandt MC, Andersen HA. The permease homologue Ssy1p controls the expression of amino acid and peptide transporter genes in Saccharomyces cerevisiae. Mol Microbiol 1998; 27: 643–650
  • Iraqui I, Vissers S, Bernard F, de Craene J-O, Boles E, Urrestarazu A, André B. Amino acid signaling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease. Mol Cell Biol 1999; 19: 989–1001
  • Gaber RF, Ottow K, Andersen HA, Kielland-Brandt MC. Constitutive and hyperresponsive signaling by mutant forms of Saccharomyces cerevisiae amino acid sensor Ssy1. Eukaryot Cell 2003; 2: 922–929
  • Wu B, Ottow K, Poulsen P, Gaber RF, Albers E, Kielland-Brandt MC. Competitive intra- and extracellular nutrient sensing by the transporter homologue Ssy1p. J Cell Biol 2006; 173: 327–331
  • Abdel-Sater F, El Bakkoury M, Urrestarazu A, Vissers S, André B. Amino acid signalling in yeast: Casein kinase I and the Ssy5 endoprotease are key determinants of endoproteolytic activation of the membrane-bound Stp1 transcription factor. Mol Cell Biol 2004; 24: 9771–9785
  • Andréasson C, Heesen S, Ljungdahl PO. Regulation of transcription factor latency by receptor-activated proteolysis. Genes Dev 2006; 20: 1563–1568
  • Poulsen P, Lo Leggio L, Kielland-Brandt MC. Mapping of an internal protease cleavage site in the Ssy5p component of the amino acid sensor of Saccharomyces cerevisae and functional characterization of the resulting pro- and protease domains by gain-of-function genetics. Eukaryot Cell 2006; 5: 601–608
  • Andréasson C, Ljungdahl PO. Receptor-mediated endoproteolytic activation of two transcription factors in yeast. Genes Dev 2002; 16: 3158–3172
  • Andréasson C, Ljungdahl PO. The N-terminal regulatory domain of Stp1p is modular and fused to an artificial transcription factor confers full SPS-sensor control. Mol Cell Biol 2004; 24: 7503–7513
  • Sobczak I, Lolkema JS. Structural and mechanistic diversity of secondary transporters. Curr Opin Microbiol 2005; 8: 161–167
  • Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S. Structure and mechanism of the lactose permease of Escherichia coli. Science 2003; 301: 610–615
  • Huang Y, Lemieux MJ, Song J, Auer M, Wang D. Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli. Science 2003; 301: 616–620
  • Yamashita A, Singh SK, Kawate T, Jin Y, Gouaux E. Crystal structure of a bacterial homologue of Na+/Cl− -dependent neurotransmitter transporters. Nature 2005; 437: 216–223
  • Boyd AC, Davidson H, Stevenson B, McLachlan G, Davidson-Smith HH, Porteous DJ. pSURF-2, a modified BAC vector for selective YAC cloning and functional analysis. Biotechniques 1999; 27: 164–170, 172, 175
  • Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning, a laboratory manual2nd edn. Cold Spring Harbor Laboratory Press, New York 1989
  • Poulsen P, Wu B, Gaber RF, Ottow K, Andersen HA, Kielland-Brandt MC. Amino acid sensing by Ssy1. Biochem Soc Trans 2005; 33: 261–264
  • Corpet F. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res 1988; 16: 10881–10890
  • Jaroszewski L, Rychlewski L, Li Z, Li W, Godzik A. 2005. FFAS03: a server for profile-profile sequence alignments. Nucleic Acids Res 33: Web Server issue W284–W288.
  • Beuming T, Shi L, Javitch JA, Weinstein H. A comprehensive structure-based alignment of prokaryotic and eukaryotic neurotransmitter/Na+ symporters (NSS) aids in the use of the LeuT structure to probe NSS structure and function. Mol Pharmacol 2006; 70: 1630–1642
  • Didion T, Grauslund M, Kielland-Brandt MC, Andersen HA. Amino acids induce expression of BAP2, a branched-chain amino acid permease gene in Saccharomyces cerevisiae. J Bacteriol 1996; 178: 2025–2029
  • Guan L, Kaback HR. Lessons from lactose permease. Annu Rev Biophys Biomol Struct 2006; 35: 67–91
  • Mirza O, Guan L, Verner L, Iwata S, Kaback HR. Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY. EMBO J 2006; 25: 1177–1183
  • Stephan MM, Chen MA, Penado KM, Rudnick G. An extracellular loop region of the serotonin transporter may be involved in the translocation mechanism. Biochemistry 1997; 36: 1322–1328
  • Liu Z, Stevens BR, Feldman DH, Hediger MA, Harvey WR. K+ amino acid transporter KAAT1 mutant Y147F has increased transport activity and altered substrate selectivity. J Exp Biol 2003; 206: 245–254
  • Hu L, King SC. Functional sensitivity of polar surfaces on transmembrane helix 8 and cytoplasmic loop 8–9 of the Escherichia coli GABA (4-aminobutyrate) transporter encoded by gabP: mutagenic analysis of a consensus amphipathic region found in transporters from bacteria to mammals. Biochem J 1998; 330: 771–776
  • Eckert-Boulet N, Larsson K, Wu B, Poulsen P, Regenberg B, Nielsen J, Kielland-Brandt MC. Deletion of RTS1, encoding a regulatory subunit of protein phosphatase 2A, results in constitutive amino acid signalling via increased Stp1p processing. Eukaryot Cell 2006; 5: 174–179
  • Spielewoy N, Flick K, Kalashnikova TI, Walker JR, Wittenberg C. Regulation and recognition of SCFGrr1 targets in the glucose and amino acid signaling pathways. Mol Cell Biol 2004; 24: 8994–9005
  • Andréasson C, Ljungdahl PO. Receptor-mediated endoproteolytic activation of two transcription factors in yeast. Genes Dev 2002; 16: 3158–3172
  • Boyd AC, Davidson H, Stevenson B, McLachlan G, Davidson-Smith HH, Porteous DJ. pSURF-2, a modified BAC vector for selective YAC cloning and functional analysis. Biotechniques 1999; 27: 164–170, 172, 175
  • Erdeniz N, Mortensen UH, Rothstein R. Cloning-free PCR-based allele replacements methods. Genome Res 1997; 7: 1174–1183
  • Gaber RF, Ottow K, Andersen HA, Kielland-Brandt MC. Constitutive and hyperresponsive signaling by mutant forms of Saccharomyces cerevisiae amino acid sensor Ssy1. Eukaryot Cell 2003; 2: 922–929
  • Jørgensen MU, Bruun MB, Didion T, Kielland-Brandt MC. Mutations in five loci affecting GAP1-independent uptake of neutral amino acids in yeast. Yeast 1998; 14: 103–114
  • Poulsen P, Wu B, Gaber RF, Kielland-Brandt MC. Constitutive signal transduction by mutant Ssy5p and Ptr3p components of the SPS amino acid sensor system in Saccharomyces cerevisiae. Eukaryot Cell 2005b; 4: 1116–1124
  • Poulsen P, Lo Leggio L, Kielland-Brandt MC. Mapping of an internal protease cleavage site in the Ssy5p component of the amino acid sensor of Saccharomyces cerevisae and functional characterization of the resulting pro- and protease domains by gain-of-function genetics. Eukaryot Cell 2006; 5: 601–608
  • Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 1989; 122: 19–27

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