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Relative substrate affinities of wild-type and mutant forms of the Escherichia coli sugar transporter GalP determined by solid-state NMR

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Pages 474-484 | Received 22 May 2008, Published online: 09 Jul 2009

References

  • Henderson PJF. Function and structure of membrane transport proteins. Transporters factsbook, JK Griffith, CE Sansom. Academic Press, London 1997; 3–29
  • Hoch JA, Silhavy TJ. 1995. Two-component signal transduction. Washington, DC: ASM Press.
  • Weber J, Senior AE. ATP synthesis driven by proton transport in F1F0-ATP synthase. Febs Lett 2003; 545: 61–70
  • Saidijam M, Benedetti G, Ren QH, Xu ZQ, Hoyle CJ, Palmer SL, Ward A, Bettaney KE, Szakonyi G, Meuller J, Morrison S, Pos MK, Butaye P, Walravens K, Langton K, Herbert RB, Skurray RA, Paulsen IT, O'Reilly J, Rutherford NG, Brown MH, Bill RM, Henderson PJF. Microbial drug efflux proteins of the major facilitator superfamily. Curr Drug Targets 2006; 7: 793–811
  • Membrane proteins of known 3D structure [Internet]. ,Irvine: Department of Physiology and Biophysics Stephen White Laboratory at UC [cited 2008 May 16]. Available from: http://blanco.biomol.uci.edu/Membrane_Proteins_xtal.html.
  • Basting D, Lehner I, Lorch M, Glaubitz C. Investigating transport proteins by solid state NMR. N-S Arch Pharmacol 2006; 372: 451–464
  • Patching SG, Brough AR, Herbert RB, Rajakarier JA, Henderson PJF, Middleton DA. Substrate affinities for membrane transport proteins determined by 13C cross-polarization magic-angle spinning nuclear magnetic resonance spectroscopy. J Am Chem Soc 2004; 126: 3072–3080
  • Boland MP, Middleton DA. Insights into the interactions between a drug and a membrane protein target by fluorine cross-polarization magic angle spinning NMR. Magn Reson Chem 2004; 42: 204–211
  • Spooner PJR, Rutherford NG, Watts A, Henderson PJF. NMR observation of substrate in the binding-site of an active sugar-H+ symport protein in native membranes. Proc Natl Acad Sci USA 1994; 91: 3877–3881
  • Pao SS, Paulsen IT, Saier MH, Jr. Major facilitator superfamily. Microbiol Mol Biol Rev 1998; 62: 1–34
  • Baldwin SA, Henderson PJF. Homologies between sugar transporters from eukaryotes and prokaryotes. Ann Rev Physiol 1989; 51: 459–471
  • Psakis G. 2004. The D-galactose-H+ symporter (GalP) from Escherichia coli: Structure-activity relationships [Thesis]. University of Leeds, UK. Available from University of Leeds library.
  • Wang D, Kranz-Eble P, De Vivo DC. Mutational analysis of GLUT1 (SLC2A1) in Glut-1 deficiency syndrome. Hum Mutat 2000; 16: 224–231
  • Maiden MC, Davis EO, Baldwin SA, Moore DC, Henderson PJF. Mammalian and bacterial sugar-transport proteins are homologous. Nature 1987; 325: 641–643
  • Cairns MT, McDonald TP, Horne P, Henderson PJF, Baldwin SA. Cytochalasin B as a probe of protein-structure and substrate recognition by the galactose/H+ transporter of Escherichia coli. J Biol Chem 1991; 266: 8176–8183
  • McDonald TP, Walmsley AR, Henderson PJF. Asparagine 394 in putative helix 11 of the galactose-H+ symport protein (GalP) from Escherichia coli is associated with the internal binding site for cytochalasin B and sugar. J Biol Chem 1997; 272: 15189–15199
  • Litherland GJ. 1996. Substrate and inhibitor recognition by chimaeric sugar-H+ transport proteins of E. coli [Thesis]. University of Leeds, UK. Available from University of Leeds library.
  • Ward A, Sanderson NM, O'Reilly J, Rutherford NG, Poolman B, Henderson PJF. The amplified expression, identification, purification, assay and properties of hexahistidine-tagged bacterial membrane transport proteins. Membrane transport – a practical approach, SA Baldwin. Blackwell, Oxford 2000; 141–166
  • Schaffner W, Weissmann C. Rapid, sensitive and specific method for determination of protein in dilute-solution. Anal Biochem 1973; 56: 502–514
  • Wu X, Zilm KW. Complete spectral editing in CPMAS NMR. J Magn Reson 1993; 102: 205–213
  • Fiser AS, Sali A. MODELLER: Generation and refinement of homology-based protein structure models. Method Enzymol 2003; 374: 461–491
  • Huang Y, Lemieux MJ, Song J, Auer M, Wang D-N. Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli. Science 2003; 301: 616–620
  • Davis IW, Leaver-Fay A, Chen VB, Block JN, Kapral GJ, Wang X, Murray LW, Arendall WB 3rd, Snoeyink J, Richardson JS, Richardson DC. MolProbity: All-atom contacts and structure validation for proteins and nucleic acids. Nucleic Acids Res 2007; 35: W375–383
  • DeLano WL. 2002. The PyMOL Molecular Graphics System. DeLano Scientific, Palo Alto, CAUSA. Available from the website: http://www.pymol.org.
  • Angyal SJ. Composition and conformation of sugars in solution. Angew Chem Int Edit 1969; 8: 157–226
  • Spooner PJR, O'Reilly WJ, Homans SW, Rutherford NG, Henderson PJF, Watts A. Weak substrate binding to transport proteins studied by NMR. Biophys J 1998; 75: 2794–2800
  • Xie H, Patching SG, Gallagher MP, Litherland GJ, Brough AR, Venter H, Yao SYM, Ng AML, Young JD, Herbert RB, Henderson PJF, Baldwin SA. Purification and properties of the Escherichia coli nucleoside transporter NupG, a paradigm for a major facilitator transporter sub-family. Mol Memb Biol 2004; 21: 323–336
  • Appleyard AN, Herbert RB, Henderson PJF, Watts A, Spooner PJR. Selective NMR observation of inhibitor and sugar binding to the galactose-H+ symport protein GalP, of Escherichia coli. Biochim Biophys Acta 2000; 1509: 55–64
  • Patching SG, Herbert RB, O'Reilly J, Brough A, Henderson PJF. Low 13C-background for NMR-based studies of ligand binding using 13C-depleted glucose as carbon source for microbial growth: 13C -labeled glucose and 13C-forskolin binding to the galactose-H+ symport protein GaIP in Escherichia coli. J Am Chem Soc 2004; 126: 86–87
  • Patching SG, Henderson PJF, Herbert RB, Middleton DA. Solid-state NMR spectroscopy detects interactions between tryptophan residues of the E. coli sugar transporter GaIP and the alpha-anomer of the D-glucose substrate. J Am Chem Soc 2008; 130: 1236–1244
  • Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S. Structure and mechanism of the lactose permease of Escherichia coli. Science 2003; 301: 610–615
  • Henderson PJF. The homologous glucose-transport proteins of prokaryotes and eukaryotes. Res Microbiol 1990; 141: 316–328
  • Sato M, Mueckler MA. A conserved amino acid motif (R-X-G-R-R) in the GLUT1 glucose transporter is an important determinant of membrane topology. J Biol Chem 1999; 274: 24721–24725
  • McDonald TP, Walmsley AR, Martin GEM, Henderson PJF. The role of tryptophans 371 and 395 in the binding of antibiotics and the transport of sugars by the D-galactose-H+ symport protein (GalP) from Escherichia coli. J Biol Chem 1995; 270: 30359–30370

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