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Altered pore properties and kinetic changes in mutants of the Vibrio cholerae Porin OmpU

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Pages 498-505 | Received 28 Jun 2008, Published online: 09 Jul 2009

References

  • Nikaido H. Molecular basis of bacterial outer membrane permeability revisited. Microbiol Mol Biol Rev 2003; 67: 593–656
  • Nikaido H. Outer membrane. Escherichia coli and Salmonella, cellular and molecular biology, FC Neidhart. ASM Press, Washington, DC 1996; 29–47
  • Schulz GE. The structures of general porins. Bacterial and Eukaryotic porins, R Benz. Wiley-Vch Verlag GmbH & Co, Weinheim 2004; 25–40
  • Delcour AH. Structure and function of pore-forming beta-barrels from bacteria. J Mol Microbiol Biotechnol 2002; 4: 1–10
  • Delcour AH. Solute uptake through general porins. Front Biosci 2003; 8: d1055–1071
  • Karshikoff A, Spassov V, Cowan SW, Ladenstein R, Schirmer T. Electrostatic properties of two porin channels from Escherichia coli. J Mol Biol 1994; 240: 372–384
  • Hille B. Ionic channels of excitable membranes. Sinauer Associates, Sunderland, MA 1992
  • Schirmer T, Phale PS. Brownian dynamics simulation of ion flow through porin channels. J Mol Biol 1999; 294: 1159–1167
  • Im W, Roux B. Ion permeation and selectivity of OmpF porin: a theoretical study based on molecular dynamics, Brownian dynamics, and continuum electrodiffusion theory. J Mol Biol 2002; 322: 851–869
  • Bishop ND, Lea EJ, Mobasheri H, Spiro S. Altered voltage sensitivity of mutant OmpC porin channels. FEBS Lett 1996; 379: 295–298
  • Delcour AH, Adler J, Kung C. A single amino acid substitution alters conductance and gating of OmpC porin of Escherichia coli. J Membr Biol 1991; 119: 267–275
  • Van Gelder P, Saint N, Phale P, Eppens EF, Prilipov A, van Boxtel R, Rosenbusch JP, Tommassen J. Voltage sensing in the PhoE and OmpF outer membrane porins of Escherichia coli: role of charged residues. J Mol Biol 1997; 269: 468–472
  • Robertson KM, Tieleman DP. Molecular basis of voltage gating of OmpF porin. Biochem Cell Biol 2002; 80: 517–523
  • Miedema H, Vrouenraets M, Wierenga J, Eisenberg B, Schirmer T, Basle A, Meijberg W. Conductance and selectivity fluctuations in D127 mutants of the bacterial porin OmpF. Eur Biophys J 2006; 36: 13–22
  • Liu N, Delcour AH. The spontaneous gating activity of OmpC porin is affected by mutations of a putative hydrogen bond network or of a salt bridge between the L3 loop and the barrel. Protein Eng 1998; 11: 797–802
  • Liu N, Samartzidou H, Lee KW, Briggs JM, Delcour AH. Effects of pore mutations and permeant ion concentration on the spontaneous gating activity of OmpC porin. Protein Eng 2000; 13: 491–500
  • Lee SH, Hava DL, Waldor MK, Camilli A. Regulation and temporal expression patterns of Vibrio cholerae virulence genes during infection. Cell 1999; 99: 625–634
  • Klose KE. Regulation of virulence in Vibrio cholerae. Int J Med Microbiol 2001; 291: 81–88
  • Provenzano D, Lauriano CM, Klose KE. Characterization of the role of the ToxR-modulated outer membrane porins OmpU and OmpT in Vibrio cholerae virulence. J Bacteriol 2001; 183: 3652–3662
  • Provenzano D, Klose KE. Altered expression of the ToxR-regulated porins OmpU and OmpT diminishes Vibrio cholerae bile resistance, virulence factor expression, and intestinal colonization. Proc Natl Acad Sci USA 2000; 97: 10220–10224
  • Simonet VC, Baslé A, Klose KE, Delcour AH. The Vibrio cholerae porins OmpU and OmpT have distinct channel properties. J Biol Chem 2003; 278: 17539–17545
  • Duret G, Delcour AH. Deoxycholic acid blocks Vibrio cholerae OmpT but not OmpU porin. J Biol Chem 2006; 281: 19899–19905
  • Duret G, Simonet V, Delcour AH. Modulation of Vibrio cholerae porin function by acidic pH. Channels 2007; 1: 70–79
  • Federici L, Du D, Walas F, Matsumura H, Fernandez-Recio J, McKeegan KS, Borges-Walmsley MI, Luisi BF, Walmsley AR. The crystal structure of the outer membrane protein VceC from the bacterial pathogen Vibrio cholerae at 1.8 Å resolution. J Biol Chem 2005; 280: 15307–15314
  • Pagel M, Simonet V, Li J, Lallemand M, Lauman B, Delcour AH. Phenotypic characterization of pore mutants of the Vibrio cholerae porin OmpU. J Bacteriol 2007; 189: 8593–8600
  • Delcour AH, Martinac B, Adler J, Kung C. Modified reconstitution method used in patch-clamp studies of Escherichia coli ion channels. Biophys J 1989; 56: 631–636
  • Liu N. 1999. Structure-function relationships of E. coli OmpC porin – the effects of site-directed mutations on the porin channel function. PhD Thesis, University of Houston, USA.
  • Phale PS, Philippsen A, Widmer C, Phale VP, Rosenbusch JP, Schirmer T. Role of charged residues at the OmpF porin channel constriction probed by mutagenesis and simulation. Biochemistry 2001; 40: 6319–6325
  • Saint N, Lou KL, Widmer C, Luckey M, Schirmer T, Rosenbusch JP. Structural and functional characterization of OmpF porin mutants selected for larger pore size. II. Functional characterization. J Biol Chem 1996; 271: 20676–20680
  • Alcaraz A, Nestorovich EM, Aguilella-Arzo M, Aguilella VM, Bezrukov SM. Salting out the ionic selectivity of a wide channel: the asymmetry of OmpF. Biophys J 2004; 87: 943–957
  • Lakey JH, Lea EJ, Pattus F. OmpC mutants which allow growth on maltodextrins show increased channel size and greater voltage sensitivity. FEBS Lett 1991; 278: 31–34
  • Vrouenraets M, Wierenga J, Meijberg W, Miedema H. Chemical modification of the bacterial porin OmpF: gain of selectivity by volume reduction. Biophys J 2006; 90: 1202–1211
  • Nestorovich EM, Rostovtseva TK, Bezrukov SM. Residue ionization and ion transport through OmpF channels. Biophys J 2003; 85: 3718–3729
  • Baslé A, Iyer R, Delcour AH. Subconductance states in OmpF gating. Biochim Biophys Acta 2004; 1664: 100–107
  • Vidal S, Bredin J, Pagès JM, Barbe J. Beta-lactam screening by specific residues of the OmpF eyelet. J Med Chem 2005; 48: 1395–1400
  • Bredin J, Saint N, Mallea M, D E, Molle G, Pages JM, Simonet V. 2002. Alteration of pore properties of Escherichia coli OmpF induced by mutation of key residues in anti-loop 3 region Biochem J 363:521–528.
  • Humphrey W, Dalke A, Schulten K. VMD – Visual Molecular Dynamics. J Mol Graphics 1996; 14: 33–38

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