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Original Article

Engineering stability in NADPH oxidases: A common strategy for enzyme production

ORCID Icon, ORCID Icon, ORCID Icon, ORCID Icon & ORCID Icon
Pages 67-76 | Received 08 May 2018, Accepted 10 Sep 2018, Published online: 10 Jan 2019

References

  • Bedard K, Krause KH. 2007. The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology. Physiol Rev 87:245–313.
  • Block K, Gorin Y. 2012. Aiding and abetting roles of NOX oxidases in cellular transformation. Nat Rev Cancer 12:627–637.
  • Brown DI, Griendling KK. 2009. Nox proteins in signal transduction. Free Radic Biol Med 47:1239–1253.
  • de Mendez I, Leto TL. 1995. Functional reconstitution of the phagocyte NADPH oxidase by transfection of its multiple components in a heterologous system. Blood 85:1104–1110.
  • Djordjevic T, BelAiba RS, Bonello S, Pfeilschifter J, Hess J, Go¨rlach A. 2005. Human urotensin II is a novel activator of NADPH oxidase in human pulmonary artery smooth muscle cells. Arterioscler Thromb Vasc Biol 25:519–525.
  • Doussiere J, Gaillard J, Vignais PV. 1999. The heme component of the neutrophil NADPH oxidase complex is a target for aryliodonium compounds. Biochemistry 38:3694–3703.
  • Fitzgerald JP, Nayak B, Shanmugasundaram K, Friedrichs W, Sudarshan S, Eid AA, et al. 2012. Nox4 mediates renal cell carcinoma cell invasion through hypoxia-induced interleukin 6- and 8- production. PLoS One 7:e30712.
  • Gao HM, Zhou H, Hong JS. 2012. NADPH oxidases: novel therapeutic targets for neurodegenerative diseases. Trends Pharmacol Sci 33:295–303.
  • Goehring A, Lee CH, Wang KH, Michel JC, Claxton DP, Baconguis I, et al. 2014. Screening and large-scale expression of membrane proteins in mammalian cells for structural studies. Nat Protoc 9:2574–2585.
  • Hattori M, Hibbs RE, Gouaux E. 2012. A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening. Structure 20:1293–1299.
  • Irani K, Xia Y, Zweier JL, Sollott SJ, Der CJ, Fearon ER, et al. 1997. Mitogenic signaling mediated by oxidants in Ras-transformed fibroblasts. Science 275:1649–1652.
  • Isogai Y, Iizuka T, Shiro Y. 1995. The mechanism of electron donation to molecular oxygen by phagocytic cytochrome b558. J Biol Chem 270:7853–7857.
  • Karplus PA, Daniels MJ, Herriott JR. 1991. Atomic structure of ferredoxin-NADP + reductase: prototype for a structurally novel flavoenzyme family. Science 251:60–66.
  • Kawate T, Gouaux E. 2006. Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins. Structure 14:673–681.
  • Kean KM, Carpenter RA, Pandini V, Zanetti G, Hall AR, Faber R, et al. 2017. High-resolution studies of hydride transfer in the ferredoxin:NADP(+) reductase superfamily. FEBS J 284:3302–3319.
  • Kuroda J, Ago T, Matsushima S, Zhai P, Schneider MD, Sadoshima J. 2010. NADPH oxidase 4 (Nox4) is a major source of oxidative stress in the failing heart. Proc Natl Acad Sci U S A 107:15565–15570.
  • Lambeth JD. 2004. NOX enzymes and the biology of reactive oxygen. Nat Rev Immunol 4:181–189.
  • Lambeth JD, Neish AS. 2014. Nox enzymes and new thinking on reactive oxygen: a double-edged sword revisited. Annu Rev Pathol 9:119–145.
  • Lord CI, Riesselman MH, Gripentrog JM, Burritt JB, Jesaitis AJ, Taylor RM. 2008. Single-step immunoaffinity purification and functional reconstitution of human phagocyte flavocytochrome b. J Immunol Methods 329:201–207.
  • Magnani F, Nenci S, Millana Fananas E, Ceccon M, Romero E, Fraaije MW, Mattevi A. 2017. Crystal structures and atomic model of NADPH oxidase. Proc Natl Acad Sci U S A 114:6764–6769.
  • Mancusso R, Karpowich NK, Czyzewski BK, Wang DN. 2011. Simple screening method for improving membrane protein thermostability. Methods 55:324–329.
  • Mittler R, Vanderauwera S, Suzuki N, Miller G, Tognetti VB, Vandepoele K, et al. 2011. ROS signaling: the new wave? Trends Plant Sci 16:300–309.
  • Nisimoto Y, Diebold BA, Cosentino-Gomes D, Lambeth JD. 2014. Nox4: a hydrogen peroxide-generating oxygen sensor. Biochemistry 53:5111–5120.
  • Nisimoto Y, Jackson HM, Ogawa H, Kawahara T, Lambeth JD. 2010. Constitutive NADPH-dependent electron transferase activity of the Nox4 dehydrogenase domain. Biochemistry 49:2433–2442.
  • O’Neill S, Brault J, Stasia M-J, Knaus UG. 2015. Genetic disorders coupled to ROS deficiency. Redox Biol 6:135–156.
  • Ogrunc M, Di Micco R, Liontos M, Bombardelli L, Mione M, Fumagalli M, et al. 2014. Oncogene-induced reactive oxygen species fuel hyperproliferation and DNA damage response activation. Cell Death Differ 21:998–1012.
  • Ohye H, Sugawara M. 2010. Dual oxidase, hydrogen peroxide and thyroid diseases. Exp Biol Med (Maywood) 235:424–433.
  • Ostuni MA, Lamanuzzi LB, Bizouarn T, Dagher MC, Baciou L. 2010. Expression of functional mammal flavocytochrome b(558) in yeast: comparison with improved insect cell system. Biochim Biophys Acta 1798:1179–1188.
  • Park HS, Chun JN, Jung HY, Choi C, Bae YS. 2006. Role of NADPH oxidase 4 in lipopolysaccharide-induced proinflammatory responses by human aortic endothelial cells. Cardiovasc Res 72:447–455.
  • Pedruzzi E, Guichard C, Ollivier V, Driss F, Fay M, Prunet C, et al. 2004. NAD(P)H oxidase Nox-4 mediates 7-ketocholesterol-induced endoplasmic reticulum stress and apoptosis in human aortic smooth muscle cells. Mol Cell Biol 24:10703–10717.
  • Rotrosen D, Yeung CL, Katkin JP. 1993. Production of recombinant cytochrome b558 allows reconstitution of the phagocyte NADPH oxidase solely from recombinant proteins. J Biol Chem 268:14256–14260.
  • Rotrosen D, Yeung CL, Leto TL, Malech HL, Kwong CH. 1992. Cytochrome b558: the flavin-binding component of the phagocyte NADPH oxidase. Science 256:1459–1462.
  • Segal AW. 1987. Absence of both cytochrome b-245 subunits from neutrophils in X-linked chronic granulomatous disease. Nature 326:88–91.
  • Tom R, Bisson L, Durocher Y. 2008. Transfection of HEK293-EBNA1 cells in suspension with linear PEI for production of recombinant proteins. CSH Protoc 2008: pdb prot4977.
  • von Lohneysen K, Noack D, Hayes P, Friedman JS, Knaus UG. 2012. Constitutive NADPH oxidase 4 activity resides in the composition of the B-loop and the penultimate C terminus. J Biol Chem 287:8737–8745.
  • Weyemi U, Lagente-Chevallier O, Boufraqech M, Prenois F, Courtin F, Caillou B, et al. 2012. ROS-generating NADPH oxidase NOX4 is a critical mediator in oncogenic H-Ras-induced DNA damage and subsequent senescence. Oncogene 31:1117–1129.
  • Zhen L, Yu L, Dinauer MC. 1998. Probing the role of the carboxyl terminus of the gp91phox subunit of neutrophil flavocytochrome b558 using site-directed mutagenesis. J Biol Chem 273:6575–6581.
  • Zhu JK. 2016. Abiotic stress signaling and responses in plants. Cell 167:313–324.

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