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Research Article

Isolation and characterization of highly purified mitochondrial outer membranes of the yeast Saccharomyces cerevisiae (Method)

Pages 205-211 | Published online: 09 Jul 2009

References

  • Alconada, A., Gärtner, F., Hönlinger, A., Kübrich, M. and Pfanner, N., 1995, Mitochondrial receptor complex from Neurospora crassa and Saccharomyces cerevisiae. Methods in Enzymology, 260, 263 - 286.
  • Bae-Lee, M. S. and Carman, G. M., 1984, Phosphatidylserine synthesis in Saccharomyces cerevisiae. Purification and char- acterization of membrane-associated phosphatidylserine synthase. Journal of Biological Chemistry, 259, 10857- 10862.
  • Bandlow, W., 1972, Membrane separation and biogenesis of the outer membrane of yeast mitochondria. Biochimica Biophysica Acta, 282, 105 - 122.
  • Bandlow, W. and Bauer, P., 1975, Separation and some properties of the inner and outer membranes of yeast mitochondria. Methods in Cell Biology, 12, 311 - 333.
  • Bligh, E. G. and Dyer, W. J., 1959, A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37, 911 - 917.
  • Boldogh, I., Vojtov, N., Karmon, S. and Pon, L. A., 1998, Interaction between mitochondria and the actin cytoskeleton in budding yeast requires two integral mitochondrial outer membrane proteins, Mmm1p and Mdm10p. Journal of Cell Biology, 141, 1371- 1381.
  • Brdiczka, D., Dölken, G., Krebs, W. and Hofmann, D., 1974, The inner boundary membrane of mitochondria. Localization and biochemical characterization, possible functions in biogenesis and metabolism. Hoppe-Seyler’s Zeitschrift für Physiologische Chemie, 355, 731 - 743.
  • Daum, G. and Vance, J. E., 1997, Import of lipids into mitochondria. Progress in Lipid Research, 36, 103 - 130.
  • Daum, G., Böhni, P. C. and Schatz, G., 1982, Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria. Journal of Biological Chemistry, 257, 13028- 13033.
  • De Kroon, A. I. P. M., Dolis, D., Mayer, A., Lill, R. and De Kruijff, B., 1997, Phospholipid composition of highly purified mitochondrial outer membranes of rat liver and Neurospora crassa. Is cardiolipin present in the mitochondrial outer membrane? Biochimica Biophysica Acta, 1325, 108 - 116.
  • Dietmeier, K., Zara, V., Palmisano, A., Palmieri, F., Voos, W., Schlossmann, J., Moczko, M., Kispal, G. and Pfanner, N., 1993, Targeting and translocation of the phosphate carrier/p32 to the inner membrane of yeast mitochondria. Journal of Biological Chemistry, 268, 25958- 25964.
  • Gaigg, B., Simbeni, R., Hrastnik, C., Paltauf, F. and Daum, G., 1995, Characterization of a microsomal subfraction associated with mitochondria of the yeast, Saccharomyces cerevisiae. Involve- ment in synthesis and import of phospholipids into mitochondria. Biochimica Biophysica Acta, 1234, 214 - 220.
  • Gallet, P. F., Petit, J.-M., Maftah, A., Zachowski, A. and Julien, R., 1997, Asymmetrical distribution of cardiolipin in yeast inner mitochondrial membrane triggered by carbon catabolite repres- sion. Biochemical Journal, 324, 627 - 634.
  • Glick, B. and Pon, L. A., 1995, Isolation of highly purified mitochondria from Saccharomyces cerevisiae. Methods in En- zymology, 260, 213 - 223.
  • Hermann, G. J., Thatcher, J. W., Mills, J. P., Hales, K. G., Fuller, M. T., Nunnari, J. and Shaw, J. M., 1998, Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p. Journal of Cell Biology, 143, 359 - 373.
  • Hill, K., Model, K., Ryan, M. T., Dietmeier, K., Martin, F., Wagner, R. and Pfanner, N., 1998, Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins. Nature, 395, 516 - 521.
  • Hines, V., Brandt, A., Griffiths, G., Horstmann, H., Brütsch, H. and Schatz, G., 1990, Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70. EMBO Journal, 9, 3191- 3200.
  • Horst, M., Jenö, P. and Kronidou, N. G., 1995, Isolation of protein import components from Saccharomyces cerevisiae mitochondria. Methods in Enzymology, 260, 232 - 241.
  • Kubota, S., Yoshida, Y., Kumaoka, H. and Furumichi, A., 1977, Studies on the microsomal electron-transport system of anaero- bically grown yeast. Purification and characterization of NADPH- cytochrome c reductase. Journal of Biochemistry, 81, 197 - 205.
  • Kuchler, K., Daum, G. and Paltauf, F., 1986, Subcellular and submitochondrial localization of phospholipid-synthesizing en- zymes in Saccharomyces cerevisiae. Journal of Bacteriology, 165, 901 - 910.
  • Mayer, A., Lill, R. and Neupert, W., 1993, Translocation and insertion of precursor proteins into isolated outer membranes of mitochon- dria. Journal of Cell Biology, 121, 1233- 1243.
  • Neupert, W., 1997, Protein import into mitochondria. Annual Reviews of Biochemistry, 66, 863 - 917.
  • Pon, L., Moll, T., Vestweber, D., Marshallsay, B. and Schatz, G., 1989, Protein import into mitochondria: ATP-dependent protein translocation activity in a submitochondrial fraction enriched in membrane contact sites and specific proteins. Journal of Cell Biology, 109, 2603- 2616.
  • Ramage, L., Junne, T., Hahne, K., Lithgow, T. and Schatz, G., 1993, Functional cooperation of mitochondrial protein import receptors in yeast. EMBO Journal, 12, 4115- 4123.
  • Rapaport, D., Brunner, M., Neupert, W. and Westermann, B., 1998, Fzo1p is a mitochondrial outer membrane protein essential for the biogenesis of functional mitochondria in Saccharomyces cerevi- siae. Journal of Biological Chemistry, 273, 20150- 20155.
  • Reed, J. C., 1997, Cytochrome c: Can’t live with it — can’t live without it. Cell, 91, 559 - 562.
  • Riezman, H., Hay, R., Gasser, S., Daum, G., Scheider, G., Witte, C. and Schatz, G., 1983, The outer membrane of yeast mitochondria: isolation of outside-out sealed vesicles. EMBO Journal, 2, 1105- 1111.
  • Rouser, G., Fleischer, S. and Yamamoto, A., 1970, Two dimensional thin layer chromatographic separation of polar lipis and determi- nation of phospholipids by phosphorus analysis of spots. Lipids, 5, 494 - 496.
  • Shen, H., Heacock, P. N., Clancey, C. J. and Dowhan, W., 1996, The CDS1 gene encoding CDP-diacylglycerol synthase in Sacchar- omyces cerevisiae is essential for cell growth. Journal of Biological Chemistry, 271, 789 - 795.
  • Shen, S.-H., Chrëtien, P., Bastien, L. and Slilaty, S. N., 1991, Primary sequence of the glucanase gene from Oerskovia xanthineolytica. Expression and purification of the enzyme from Escherichia coli. Journal of Biological Chemistry, 266, 1058- 1063.
  • Shiao, Y.-J., Lupo, G. and Vance, J. E., 1995, Evidence that phosphatidylserine is imported into mitochondria via a mitochon- dria-associated membrane and that the majority of mitochondrial phosphatidylethanolamine is derived from decarboxylation of phosphatidylserine. Journal of Biological Chemistry, 270, 11190- 11198.
  • Sogo, L. F. and Yaffe, M. P., 1994, Regulation of mitochondrial morphology and inheritance by Mdm10p, a protein of the mitochondrial outer membrane. Journal of Cell Biology, 126, 1361- 1373.
  • Sperka-Gottlieb, C. D. M., Hermetter, A., Paltauf, F. and Daum, G., 1988, Lipid topology and physical properties of the outer mitochondrial membrane of the yeast, Saccharomyces cerevisiae. Biochimica Biophysica Acta, 946, 227 - 234.
  • Stirling, C. J., Rothblatt, J., Hosobuchi, M., Deshaies, R. and Schekman, R., 1992, Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum. Molecular Biology of the Cell, 3, 129 - 142.
  • Sutter, T. R. and Loper, J. C., 1989, Disruption of the Sacchar- omyces cerevisiae gene for NADPH-cytochrome P450 reductase causes increased sensitivity to ketoconazole. Biochemical and Biophysical Research Communications, 160, 1257- 1266.
  • Tzagoloff, A., Akai, A. and Foury, F., 1976, Assembly of the mitochondrial membrane system XVI. Modified form of the ATPase proteolipid in oligomycin-resistant mutants of Sacchar- omyces cerevisiae. FEBS Letters, 65, 391 - 395.
  • Yamashita, S. and Nikawa, J.-I., 1998, Phosphatidylserine synthase from yeast. Biochimica Biophysica Acta, 1348, 228 - 235.
  • Zinser, E. and Daum, G., 1995, Isolation and biochemical char- acterization of organelles from the yeast, Saccharomyces cerevisiae. Yeast, 11, 493 - 536.
  • Zinser, E., Sperka-Gottlieb, C. D. M., Fasch, E. V., Kohlwein, S. D., Paltauf, F. and Daum, G., 1991, Phospholipid synthesis and lipid composition of subcellular membranes in the unicellular eukaryote Saccharomyces cerevisiae. Journal of Bacteriology, 173, 2026- 2034.

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