Cloning and functional characterization of two bacterial members of the NAT/NCS2 family in Escherichia coli

References

• Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S. Structure and mechanism of the lactose permease of Escherichia, coli. Science 2003; 301: 610–615
   PubMed Web of Science ®Google Scholar
• Abramson J, Iwata S, Kaback HR. Lactose permease as a paradigm for membrane transport proteins. Mol Membr Biol 2004; 21: 227–236
   PubMed Web of Science ®Google Scholar
• Amillis S, Koukaki M, Diallinas G. Substitution F569S converts UapA, a specific uric acid-xanthine transporter, into a broad specificity transporter for purine-related solutes. J Mol Biol 2001; 313: 765–774
   PubMedGoogle Scholar
• Amillis S, Cecchetto G, Sophianopoulou V, Koukaki M, Scazzocchio C, Diallinas G. Transcription of purine transporter genes is activated during the isotropic growth phase of Aspergillus, nidulans conidia. Mol Microbiol 2004; 52: 205–216
   PubMedGoogle Scholar
• Andersen PS, Frees D, Fast R, Mygind B. Uracil uptake in Escherichia, coli K-12: isolation of uraA mutants and cloning of the gene. J Bacteriol 1995; 177: 2008–2013
   PubMed Web of Science ®Google Scholar
• Argyrou E, Sophianopoulou V, Schultes N, Diallinas G. Functional characterization of a maize purine transporter by expression in Aspergillus, nidulans. Plant Cell 2001; 13: 953–964
   PubMedGoogle Scholar
• Blattner FR, Plunkett G III, Bloch CA, Perna NT, Burland V, Riley M, et al. The complete genome sequence of Escherichia, coli K-12. Science 1997; 277: 1453–1474
   PubMed Web of Science ®Google Scholar
• Burton K. Transport of nucleic acid bases into Escherichia, coli. J Gen Microbiol 1983; 129: 3505–3513
   PubMedGoogle Scholar
• Carrasco N, Herzlinger D, Mitchell R, DeChiara S, Danho W, Gabriel TF, Kaback HR. Intramolecular dislocation of the COOH terminus of the lac carrier protein in reconstituted proteoliposomes. Proc Natl Acad Sci USA 1984; 81: 4672–4676
   PubMedGoogle Scholar
• Casadaban MJ, Cohen SN. Analysis of gene control signals by DNA fusion and cloning in Escherichia, coli. J Mol Biol 1980; 138: 179–207
   PubMed Web of Science ®Google Scholar
• Christiansen LC, Schou S, Nygaard P, Saxild HH. Xanthine metabolism in Bacillus, subtilis: Characterization of the xpt-pbuX operon and evidence for purine- and nitrogen-controlled expression of genes involved in xanthine salvage and catabolism. J Bacteriol 1997; 179: 2540–2550
   PubMed Web of Science ®Google Scholar
• Consler TG, Persson BL, Jung H, Zen KH, Jung K, Privé GG, Verner GE, Kaback HR. Properties and purification of an active biotinylated lactose permease from Escherichia, coli. Proc Natl Acad Sci USA 1993; 90: 6934–6938
   PubMed Web of Science ®Google Scholar
• Datsenko KA, Wanner BL. One-step inactivation of chromosomal genes in Escherichia, coli K-12 using PCR products. Proc Natl Acad Sci USA 2000; 97: 6640–6645
   PubMed Web of Science ®Google Scholar
• de Koning H, Diallinas G. Nucleobase transporters. Mol Membr Biol 2000; 17: 75–94
   PubMed Web of Science ®Google Scholar
• Diallinas G, Scazzocchio C. A gene coding for the uric acid-xanthine permease of Aspergillus, nidulans: inactivational cloning, characterization and sequence of a cis-acting mutation. Genetics 1989; 122: 341–350
   PubMed Web of Science ®Google Scholar
• Diallinas G, Gorfinkiel L, Arst HN Jr, Cecchetto G, Scazzocchio C. Genetic and molecular characterization of a gene encoding a wide specificity purine permease of Aspergillus, nidulans reveals a novel family of transporters conserved in prokaryotes and eukaryotes. J Biol Chem 1995; 270: 8610–8622
   PubMed Web of Science ®Google Scholar
• Diallinas G, Valdez J, Sophianopoulou V, Rosa A, Scazzocchio C. Chimeric purine transporters of Aspergillus, nidulans define a domain critical for function and specificity conserved in bacterial, plant and metazoan homologues. EMBO J 1998; 17: 3827–3837
   PubMedGoogle Scholar
• Drew D, Sjostrand D, Nilsson J, Urbig T, Chin CN, de Gier JW, von Heijne G. Rapid topology mapping of Escherichia, coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis. Proc Natl Acad Sci USA 2002; 99: 2690–2695
   PubMedGoogle Scholar
• Felle H, Porter JS, Slayman CL, Kaback HR. Quantitative measurements of membrane potential in Escherichia, coli. Biochemistry 1980; 19: 3585–3590
   PubMedGoogle Scholar
• Frillingos S, Kaback HR. Altering sugar transport specificity of a bacterial oligosaccharide-H+ symporter (OHS) by site-directed mutagenesis. Eur J Biochem 2001; 268: 223
   Google Scholar
• Frillingos S, Sahin-Tóth M, Persson B, Kaback HR. Cysteine-scanning mutagenesis of putative helix VII in lactose permease of Escherichia, coli. Biochemistry 1994; 33: 8074–8081
   PubMedGoogle Scholar
• Frillingos S, Sahin-Tóth M, Wu J, Kaback HR. Cys-scanning mutagenesis: a novel approach to structure-function relationships in membrane proteins. FASEB J 1998; 12: 1281–1299
   PubMed Web of Science ®Google Scholar
• Gorfinkiel L, Diallinas G, Scazzocchio C. Sequence and regulation of the uapA gene encoding a uric acid-xanthine permease in the fungus Aspergillus, nidulans. J Biol Chem 1993; 268: 23376–23381
   PubMedGoogle Scholar
• Goudela S, Karatza P, Koukaki M, Frillingos S, Diallinas G. Comparative substrate recognition by bacterial and fungal purine transporters of the NAT family. Mol Membr Biol 2005; 22: 12–23
   Google Scholar
• Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 1989; 77: 51–59
   PubMed Web of Science ®Google Scholar
• Inoue H, Nojima H, Okayama H. High efficiency transformation of Escherichia, coli with plasmids. Gene 1990; 96: 23–28
   PubMed Web of Science ®Google Scholar
• Iwata S, Kaback HR. Membranes: A new era in membrane protein studies. Curr Opin Struct Biol 2004; 14: 387–389
   Google Scholar
• Kaback HR, Reeves JP, Short SA, Lombardi FJ. Mechanisms of active transport in isolated bacterial membrane vesicles: XVIII. The mechanism of action of carbonylcyanide m-chlorophenylhydrazone. Arch Biochem Biophys 1974; 160: 215–222
   PubMedGoogle Scholar
• Konings WN, Barnes EM Jr, Kaback HR. Mechanisms of active transport in isolated membrane vesicles. 2. The coupling of reduced phenazine methosulphate to the concentrative uptake of beta-galactosides and amino acids. J Biol Chem 1971; 246: 5857–5861
   PubMed Web of Science ®Google Scholar
• Liang WJ, Johnson D, Jarvis SM. Vitamin C transport systems of mammalian cells. Mol Membr Biol 2001; 18: 87–95
   PubMed Web of Science ®Google Scholar
• Martinussen J, Schallert J, Andersen B, Hammer K. The pyrimidine operon pyrRPB-carA from Lactococcus, lactis. J Bacteriol 2001; 183: 2785–2794
   PubMedGoogle Scholar
• Meintanis C, Karagouni AD, Diallinas G. Amino acid residues N450 and Q449 are critical for the uptake capacity and specificity of UapA, a prototype of a nucleobase-ascorbate transporter family. Mol Membr Biol 2000; 17: 47–57
   PubMed Web of Science ®Google Scholar
• Reitzer L, Schneider BL. Metabolic context and possible physiological themes of σ54–dependent genes in Escherichia, coli. Microbiol Mol Biol Rev 2001; 65: 422–444
   PubMedGoogle Scholar
• Ren Q, Kang KH, Paulsen IT. TransportDB: A relational database of cellular membrane transport systems. Nucleic Acids Res 2004; 32: D284–D288
   Google Scholar
• Roy-Burman S, Visser DW. Transport of purines and deoxyadenosine in Escherichia, coli. J Biol Chem 1975; 250: 9270–9275
   PubMedGoogle Scholar
• Sahin-Tóth M, Frillingos S, Lengeler J W, Kaback H R. Active transport by the CscB permease in Escherichia, coli K-12. Biochem Biophys Res Com 1995; 208: 1116–1123
   PubMedGoogle Scholar
• Saier MH Jr. A functional-phylogenetic classification system for transmembrane solute transporters. Microbiol Mol Biol Rev 2000; 64: 354–411
   PubMed Web of Science ®Google Scholar
• Schultz AC, Nygaard P, Saxild HH. Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus, subtilis and evidence for a novel regulon controlled by the PucR transcription activator. J Bacteriol 2001; 183: 3293–3302
   PubMed Web of Science ®Google Scholar
• Smirnova I, Kaback HR. A mutation in the lactose permease of Escherichia, coli that decreases conformational flexibility and increases protein stability. Biochemistry 2003; 42: 3025–3031
   PubMedGoogle Scholar
• Tatusov RL, Koonin EV, Lipman DJ. A genomic perspective on protein families. Science 1997; 278: 631–637
   PubMed Web of Science ®Google Scholar
• Tavoularis S, Scazzochio C, Sophianopoulou V. Functional expression and cellular localization of a green fluorescent protein-tagged proline transporter in Aspergillus, nidulans. Fung Genet Biol 2001; 33: 115–125
   PubMed Web of Science ®Google Scholar
• Teather RM, Bramhill J, Riede I, Wright JK, Furst M, Aichele G, Wilhelm V, Overath P. Lactose carrier protein of Escherichia, coli. Structure and expression of plasmids carrying the Y gene of the lac operon. Eur J Biochem 1980; 108: 223–231
   PubMedGoogle Scholar
• Tsukaguchi H, Tokui T, Mackenzie B, Berger UV, Chen XZ, Wang Y, Brubaker RF, Hediger MA. A family of mammalian Na+-dependent L-ascorbic acid transporters. Nature 1999; 399: 70–75
   PubMed Web of Science ®Google Scholar
• Turner RJ, Lu Y, Switzer RL. Regulation of the Bacillus, subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism. J Bacteriol 1994; 176: 3708–3722
   PubMedGoogle Scholar
• Xi H, Schneider BL, Reitzer L. Purine catabolism in Escherichia, coli and function of xanthine dehydrogenase in purine salvage. J Bacteriol 2000; 182: 5332–5341
   PubMed Web of Science ®Google Scholar
• Zalkin H, Nygaard P. Biosynthesis of purine nucleosides. Escherichia, coli and Salmonella, typhimurium: Cellular and Molecular Biology2nd edn, FC Neidhardt, et al. American Society for Microbiology, Washington, DC 1996; 561–579
   Google Scholar