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Original

Substrate-induced conformational changes of the mitochondrial oxoglutarate carrier: a spectroscopic and molecular modelling study

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Pages 443-452 | Received 27 Apr 2005, Published online: 09 Jul 2009

References

  • Bisaccia F, Capobianco L, Brandolin G, Palmieri F. Transmembrane topography of the mitochondrial oxoglutarate carrier assessed by peptide-specific antibodies and enzymatic cleavage. Biochemistry 1994; 33: 3705–3713
  • Castiglione-Morelli MA, Ostuni A, Pepe A, Lauria G, Palmieri F, Bisaccia F. Solution structure of the first and second transmembrane segments of the mitochondrial oxoglutarate carrier. Mol Membr Biol 2004; 21: 297–305
  • Castiglione-Morelli MA, Ostuni A, Croce F, Palmieri F, Bisaccia F. Solution structure of the fifth and sixth transmembrane segments of the mitochondrial oxoglutarate carrier. Mol Membr Biol 2005; 22: 191–201
  • Chen Y, Barkley MD. Toward understanding tryptophan fluorescence in proteins. Biochemistry 1998; 37: 9976–9982
  • Fiermonte G, Walker JE, Palmieri F. Abundant bacterial expression and reconstitution of an intrinsic membrane-transport protein from bovine mitochondria. Biochem J 1993; 294: 293–299
  • Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 1989; 77: 51–59
  • Jezek P, Jezek J. Sequence anatomy of mitochondrial anion carriers. FEBS Lett 2003; 534: 15–25
  • Kunji ER, Harding M. Projection structure of the atractyloside-inhibited mitochondrial ADP/ATP carrier of Saccharomyces cerevisiae. J Biol Chem 2003; 278: 36985–36988
  • Lakowicz J. Principles of fluorescence spectroscopy. Kluwer Academic and Plenum Publishers, New York 1999
  • Laskowski RA, MacArthur MW, Moss DS, Thornton JM. PROCHECK: A program to check the stereochemical quality of protein structures. J Appl Cryst 1993; 26: 283–291
  • Martelli PL, Fariselli P, Casadio R. An ENSEMBLE machine learning approach for the prediction of all-alpha membrane proteins. Bioinformatics 2003; 19(Suppl. 1)i205–211
  • Morozzo della Rocca B, Lauria G, Venerini F, Palmieri L, Polizio F, Capobianco L, Stipani V, Pedersen J, Cappello AR, Desideri A, Palmieri F. The mitochondrial oxoglutarate carrier: structural and dynamic properties of transmembrane segment IV studied by site-directed spin labeling. Biochemistry 2003; 42: 5493–5499
  • Nelson DR, Felix CM, Swanson JM. Highly conserved charge-pair networks in the mitochondrial carrier family. J Mol Biol 1998; 277: 285–308
  • Palmieri F. Mitochondrial carrier proteins. FEBS Lett 1994; 346: 48–54
  • Palmieri F. The mitochondrial transporter family (SLC25): physiological and pathological implications. Pflugers Arch – Eur J Physiol 2004; 447: 689–709
  • Palmieri F, Indiveri C, Bisaccia F, Iacobazzi V. Mitochondrial metabolite carrier proteins: purification reconstitution and transport studies. Methods Enzymol 1995; 260: 349–369
  • Pebay-Peyroula E, Dahout-Gonzalez C, Kahn R, Trezeguet V, Lauquin GJ, Brandolin G. Structure of mitochondrial ADP/ATP carrier in complex with carboxyatractyloside. Nature 2003; 426: 39–44
  • Runswick MJ, Walker JE, Bisaccia F, Iacobazzi V, Palmieri F. Sequence of the bovine 2-oxoglutarate/malate carrier protein: Structural relationship to other mitochondrial transport proteins. Biochemistry 1990; 29: 11033–11040
  • Sali A, Blundell TL. Comparative protein modelling by satisfaction of spatial restraints. J Mol Biol 1993; 234: 779–815
  • Stipani V, Cappello AR, Daddabbo L, Natuzzi D, Miniero DV, Stipani I, Palmieri F. The mitochondrial oxoglutarate carrier: Cysteine-scanning mutagenesis of transmembrane domain IV and sensitivity of Cys mutants to sulfhydryl reagents. Biochemistry 2001; 40: 15805–15810
  • Vriend G. WHAT IF: a molecular modelling and drug design program. J Mol Graph 1990; 8: 52–56

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