Energy-independent translocation of cell-penetrating peptides occurs without formation of pores. A biophysical study with pep-1

References

• Magzoub M, Graslund A. Cell-penetrating peptides: from inception to application. Q Rev Biophys 2004; 37: 147–195
   PubMed Web of Science ®Google Scholar
• Thoren PE, Persson D, Isakson P, Goksor M, Onfelt A, Norden B. Uptake of analogs of penetratin, Tat(48-60) and oligoarginine in live cells. Biochem Biophys Res Commun 2003; 307: 100–107
   PubMed Web of Science ®Google Scholar
• Richard JP, Melikov K, Vives E, Ramos C, Verbeure B, Gait MJ, Chernomordik LV, Lebleu B. Cell-penetrating peptides. A reevaluation of the mechanism of cellular uptake. J Biol Chem 2003; 278: 585–590
   PubMed Web of Science ®Google Scholar
• Drin G, Cottin S, Blanc E, Rees AR, Temsamani J. Studies on the internalization mechanism of cationic cell-penetrating peptides. J Biol Chem 2003; 278: 31192–31201
   PubMed Web of Science ®Google Scholar
• Morris MC, Depollier J, Mery J, Heitz F, Divita G. A peptide carrier for the delivery of biologically active proteins into mammalian cells. Nat Biotechnol 2001; 19: 1173–1176
   PubMed Web of Science ®Google Scholar
• Henriques ST, Costa J, Castanho MA. Translocation of beta-galactosidase mediated by the cell-penetrating peptide pep-1 into lipid vesicles and human HeLa cells is driven by membrane electrostatic potential. Biochemistry 2005; 44: 10189–10198
   PubMed Web of Science ®Google Scholar
• Henriques ST, Castanho MA. Consequences of nonlytic membrane perturbation to the translocation of the cell penetrating peptide pep-1 in lipidic vesicles. Biochemistry 2004; 43: 9716–9724
   PubMed Web of Science ®Google Scholar
• Henriques ST, Castanho MA. Environmental factors that enhance the action of the cell penetrating peptide pep-1 A spectroscopic study using lipidic vesicles. Biochim Biophys Acta 2005; 1669: 75–86
   PubMed Web of Science ®Google Scholar
• Henriques ST, Costa J, Castanho MA. Re-evaluating the role of strongly charged sequences in amphipathic cell-penetrating peptides: a fluorescence study using Pep-1. FEBS Lett 2005; 579: 4498–4502
   PubMed Web of Science ®Google Scholar
• Weller K, Lauber S, Lerch M, Renaud A, Merkle HP, Zerbe O. Biophysical and biological studies of end-group-modified derivatives of Pep-1. Biochemistry 2005; 44: 15799–15811
   PubMed Web of Science ®Google Scholar
• Fischer R, Fotin-Mleczek M, Hufnagel H, Brock R. Break on through to the other side –biophysics and cell biology shed light on cell-penetrating peptides. Chembiochem 2005; 6: 2126–2142
   PubMed Web of Science ®Google Scholar
• Deshayes S, Heitz A, Morris MC, Charnet P, Divita G, Heitz F. Insight into the mechanism of internalization of the cell-penetrating carrier peptide Pep-1 through conformational analysis. Biochemistry 2004; 43: 1449–1457
   PubMed Web of Science ®Google Scholar
• Vigano C, Manciu L, Buyse F, Goormaghtigh E, Ruysschaert JM. Attenuated total reflection IR spectroscopy as a tool to investigate the structure, orientation and tertiary structure changes in peptides and membrane proteins. Biopolymers 2000; 55: 373–380
   PubMed Web of Science ®Google Scholar
• Zhang S, Udho E, Wu Z, Collier RJ, Finkelstein A. Protein translocation through anthrax toxin channels formed in planar lipid bilayers. Biophys J 2004; 87: 3842–3849
   PubMed Web of Science ®Google Scholar
• Ambroggio EE, Separovic F, Bowie JH, Fidelio GD, Bagatolli LA. Direct visualization of membrane leakage induced by the antibiotic peptides: maculatin, citropin, and aurein. Biophys J 2005; 89: 1874–1881
   PubMed Web of Science ®Google Scholar
• Mayer LD, Hope MJ, Cullis PR. Vesicles of variable sizes produced by a rapid extrusion procedure. Biochim Biophys Acta 1986; 858: 161–168
   PubMed Web of Science ®Google Scholar
• Yang JT, Wu CS, Martinez HM. Calculation of protein conformation from circular dichroism. Methods Enzymol 1986; 130: 208–269
   PubMed Web of Science ®Google Scholar
• Goormaghtigh E, Raussens V, Ruysschaert JM. Attenuated total reflection infrared spectroscopy of proteins and lipids in biological membranes. Biochim Biophys Acta 1999; 1422: 105–185
   PubMed Web of Science ®Google Scholar
• Torrecillas A, Martinez-Senac MM, Goormaghtigh E, de Godos A, Corbalan-Garcia S, Gomez-Fernandez JC. Modulation of the membrane orientation and secondary structure of the C-terminal domains of Bak and Bcl-2 by lipids. Biochemistry 2005; 44: 10796–10809
   PubMed Web of Science ®Google Scholar
• Goormaghtigh E, Cabiaux V, Ruysschaert JM. Determination of soluble and membrane protein structure by Fourier transform infrared spectroscopy. I. Assignments and model compounds. Subcell Biochem 1994; 23: 329–362
   PubMedGoogle Scholar
• Arrondo JL, Goni FM. Structure and dynamics of membrane proteins as studied by infrared spectroscopy. Prog Biophys Mol Biol 1999; 72: 367–405
   PubMed Web of Science ®Google Scholar
• Barth A, Zscherp C. What vibrations tell us about proteins. Q Rev Biophys 2002; 35: 369–430
   PubMed Web of Science ®Google Scholar
• Tatulian SA. Attenuated total reflection Fourier transform infrared spectroscopy: a method of choice for studying membrane proteins and lipids. Biochemistry 2003; 42: 11898–11907
   PubMed Web of Science ®Google Scholar
• Bechinger B, Ruysschaert JM, Goormaghtigh E. Membrane helix orientation from linear dichroism of infrared attenuated total reflection spectra. Biophys J 1999; 76: 552–563
   PubMed Web of Science ®Google Scholar
• Montal M, Mueller P. Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc Natl Acad Sci USA 1972; 69: 3561–3566
   PubMed Web of Science ®Google Scholar
• Fuks B, Homble F. Permeability and electrical properties of planar lipid membranes from thylakoid lipids. Biophys J 1994; 66: 1404–1414
   PubMed Web of Science ®Google Scholar
• Redwood WR, Pfeiffer FR, Weisbach JA, Thompson TE. Physical properties of bilayer membranes formed from a synthetic saturated phospholipid in n-decane. Biochim Biophys Acta 1971; 233: 1–6
   PubMed Web of Science ®Google Scholar
• Lindsey H, Petersen NO, Chan SI. Physicochemical characterization of 1,2-diphytanoyl-sn-glycero-3-phosphocholine in model membrane systems. Biochim Biophys Acta 1979; 555: 147–167
   PubMed Web of Science ®Google Scholar
• Hung WC, Chen FY, Huang HW. Order-disorder transition in bilayers of diphytanoyl phosphatidylcholine. Biochim Biophys Acta 2000; 1467: 198–206
   PubMed Web of Science ®Google Scholar
• Ambroggio EE, Kim DH, Separovic F, Barrow CJ, Barnham KJ, Bagatolli LA, Fidelio GD. Surface behavior and lipid interaction of Alzheimer beta-amyloid peptide 1-42: a membrane-disrupting peptide. Biophys J 2005; 88: 2706–2713
   PubMed Web of Science ®Google Scholar
• Thoren PE, Persson D, Lincoln P, Norden B. Membrane destabilizing properties of cell-penetrating peptides. Biophys Chem 2005; 114: 169–179
   PubMed Web of Science ®Google Scholar
• Sreerama N, Venyaminov SY, Woody RW. Estimation of the number of alpha-helical and beta-strand segments in proteins using circular dichroism spectroscopy. Protein Sci 1999; 8: 370–380
   PubMed Web of Science ®Google Scholar
• Woody RW. Circular dichroism. Methods Enzymol 1995; 246: 34–71
   PubMed Web of Science ®Google Scholar
• Oberg KA, Ruysschaert JM, Goormaghtigh E. The optimization of protein secondary structure determination with infrared and circular dichroism spectra. Eur J Biochem 2004; 271: 2937–2948
   PubMedGoogle Scholar
• Wieprecht T, Beyermann M, Seelig J. Thermodynamics of the coil-alpha-helix transition of amphipathic peptides in a membrane environment: the role of vesicle curvature. Biophys Chem 2002; 96: 191–201
   PubMed Web of Science ®Google Scholar
• Yang L, Harroun TA, Weiss TM, Ding L, Huang HW. Barrel-stave model or toroidal model? A case study on melittin pores. Biophys J 2001; 81: 1475–1485
   PubMed Web of Science ®Google Scholar
• Thulstrup EW, Michl J. Elementary polarization spectroscopy. VCH Publishers; 52 pp, New York 1989
   Google Scholar
• Shai Y. Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by alpha-helical antimicrobial and cell non-selective membrane-lytic peptides. Biochim Biophys Acta 1999; 1462: 55–70
   PubMed Web of Science ®Google Scholar
• Wiener MC, White SH. Structure of a fluid dioleoylphosphatidylcholine bilayer determined by joint refinement of x-ray and neutron diffraction data. III. Complete structure. Biophys J 1992; 61: 434–447
   PubMed Web of Science ®Google Scholar
• Herbig ME, Weller K, Krauss U, Beck-Sickinger AG, Merkle HP, Zerbe O. Membrane surface-associated helices promote lipid interactions and cellular uptake of human calcitonin-derived cell penetrating peptides. Biophys J 2005; 89: 4056–4066
   PubMed Web of Science ®Google Scholar
• Ludtke S, He K, Huang H. Membrane thinning caused by magainin 2. Biochemistry 1995; 34: 16764–16769
   PubMed Web of Science ®Google Scholar