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Original Articles

β-Tubulin Gene Based PCR-RFLP Method for Specific Detection of Babesia bigemina and Theileria annulata Isolates

, , , , , , & show all
Pages 233-238 | Received 19 May 2009, Accepted 22 Dec 2009, Published online: 14 Nov 2011

References

  • Singh , H. , Cheema , P. S. , Mishra , A. K. , Tewari , A. K. , Rao , J. R. , Ravindran , R. , Ray , D. and Bansal , G. C. 2010 . β-tubulin gene based PCR-RFLP method for specific detection of Babesia bigemina and Theileria annulata isolates . J. Appl. Anim. Res. , 37 : 233 – 238 .
  • Aktas , M. , Altay , K. and Dumanli , N. 2006 . A molecular survey of bovine Theileria parasites among apparently healthy cattle and with a note on the distribution of ticks in eastern Turkey . Vet. Parasitol. , 138 : 179 – 185 .
  • Caccio , S. , Camma , C. , Onuma , M. and Severini , C. 2000 . The β-tubulin gene of Babesia and Theileria parasites is an informative marker for species discrimination . Int. J. Parasitol. , 30 : 1181 – 1185 .
  • Ravindran , R. , Mishra , A. K. and Rao , J. R. 2002 . On the high seroprevalence of bovine babesiosis in Wayanad district of Kerala . J. Appl. Anim. Res. , 22 : 43 – 48 .
  • Ray , D. , Bansal , G. C. and Dutta , B. 1998 . Purification of intraerythrocytic piroplasms of Theileria annulata from infected bovine blood . Indian J. Anim. Sci. , 68 : 1167 – 1168 .
  • Sambrook , J. , Fritsch , E. F. and Maniatis , T. 1989 . Molecular Cloning: A Laboratory Manual , vol. 2 , 9.16 – 9.22 . New York : Cold Spring Harbor Laboratory, Cold Spring Harbor .
  • Sanger , F. , Nickien , S. and Coulson , A. R. 1977 . DNA sequencing with chain terminator inhibitors . Proc. Natl. Acad. Sci. USA. , 74 : 5463 – 5467 .
  • Singh , H. , Mishra , A. K. , Rao , J. R. and Tewari , A. K. 2007a . Seroprevalence of babesiosis in cattle and buffaloes by indirect fluorescent antibody test . J Vet. Parasitol. , 21 : 1 – 4 .
  • Singh , H. , Mishra , A. K. , Rao , J. R. , Cheema , P. S. and Sankar , M. 2007b . Polymerase chain reaction for diagnosis of Babesia bigemina . Indian Vet. J. , 84 : 346 – 348 .
  • Singh , H. , Mishra , A. K. , Rao , J. R. and Tewari , A. K. 2007c . A PCR assay for detection of Babesia bigemina infection using clotted blood in bovines . J. Appl. Anim. Res. , 32 : 201 – 202 .

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