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ORIGINAL ARTICLE

Crystal structure of the Glu328Gln mutant of Neisseria polysaccharea amylosucrase in complex with sucrose and maltoheptaose

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Pages 99-105 | Received 20 May 2005, Published online: 11 Jul 2009

References

  • Albenne C, De Montalk GP, Monsan P, Skov L, Mirza O, Gajhede M, Remaud-Simeon M. Site-directed mutagenesis of key amino acids in the active site of amylosucrase from Neisseria polysaccharea. Biologia 2002a; 57: 119–128
  • Albenne C, Skov LK, Mirza O, Gajhede M, Potocki-Veronese G, Monsan P, Remaud-Simeon M. Maltooligosaccharide disproportionation reaction: an intrinsic property of amylosucrase from Neisseria polysaccharea. FEBS Lett 2002b; 527: 67–70
  • Albenne C, van der Veen BA, Potocki-Veronese G, Joucla G, Skov L, Mirza O, Gajhede M, Monsan P, Remaud-Simeon M. Rational and combinatorial engineering of the glucan synthesizing enzyme amylosucrase. Biocat Biotrans 2003; 21: 271–277
  • Albenne C, Skov LK, Mirza O, Gajhede M, Feller G, D'Amico S, Andre G, Potocki-Veronese G, van der Veen BA, Monsan P, Remaud-Simeon M. Molecular Basis of the Amylose-like Polymer Formation Catalyzed by Neisseria polysaccharea Amylosucrase. J Biol Chem 2004; 279: 726–734
  • Brunger AT, Adams PD, Clore GM, DeLano WL, Gros P, Grosse-Kunstleve RW, Jiang JS, Kuszewski J, Nilges M, Pannu NS, et al. Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr 1998; D54: 905–921
  • Buttcher V, Welsh T, Willmitzer L, Kossmann J. Cloning and characterization of the gene for amylosucrase from Neisseria polysaccharea: Production of a linear alpha-1,4- glucan. J Bacteriol 1997; 179: 3324–3330
  • Cortes J, Simeon T, Remaud-Simeon M, Tran V. Geometric algorithms for the conformational analysis of long protein loops. J Comp Chem 2004; 25: 956–967
  • Coutinho, PM, Henrissat, B. 1999. http://afmb.cnrs-mrs.fr/∼pedro/CAZY.
  • Henrissat B, Davies G. Structural and sequence-based classification of glycoside hydrolases. Curr Opin Struc Biol 1997; 7: 637–644
  • Jensen MH, Mirza O, Albenne C, Remaud-Simeon M, Monsan P, Gajhede M, Skov LK. Crystal structure of the covalent intermediate of amylosucrase from Neisseria polysaccharea. Biochemistry 2004; 43: 3104–3110
  • Jones TA, Zou JY, Cowan SW, Kjeldgaard M. Improved methods for building protein models in electron-density maps and the location of errors in these models. Acta Crystallogr 1991; A47: 110–119
  • Koshland DE. Stereochemistry and the mexhanism of enzymatic reactions. Biol Rev Camb Philos Soc 1953; 28: 416–436
  • Laskowski RA, Macarthur MW, Moss DS, Thornton JM. Procheck – a program to check the stereochemical quality of protein structures. J Appl Cryst 1993; 26: 283–291
  • Mirza O, Skov LK, Remaud-Simeon M, De Montalk GP, Albenne C, Monsan P, Gajhede M. Crystal structures of amylosucrase from Neisseria polysaccharea in complex with D-glucose and the active site mutant Glu328Gln in complex with the natural substrate sucrose. Biochemistry 2001; 40: 9032–9039
  • Otwinowski Z, Minor W. Processing of X-ray diffraction data collected in oscillation mode. Method Enzymol 1997; 276: 307–326
  • Potocki de Montalk G, Remaud-Simeon M, Willemot RM, Monsan P. Characterisation of the activator effect of glycogen on amylosucrase from Neisseria polysaccharea. FEMS Microbiol Lett 2000a; 186: 103–108
  • Potocki de Montalk G, Remaud-Simeon M, Willemot RM, Planchot V, Monsan P. Sequence analysis of the gene encoding amylosucrase from Neisseria polysaccharea and characterization of the recombinant enzyme. J Bacteriol 1999; 181: 375–381
  • Potocki de Montalk G, Remaud-Simeon M, Willemot RM, Sarcabal P, Planchot V, Monsan P. Amylosucrase from Neisseria polysaccharea: novel catalytic properties. FEBS Lett 2000b; 471: 219–223
  • Read RJ. Improved fourier coefficients for maps using phases from partial structures with errors. Acta Crystallogr 1986; A42: 140–149
  • Sarcabal P, Remaud-Simeon M, Willemot RM, De Montalk GP, Svensson B, Monsan P. Identification of key amino acid residues in Neisseria polysaccharea amylosucrase. FEBS Lett 2000; 474: 33–37
  • Skov LK, Mirza O, Henriksen A, De Montalk GP, Remaud-Simeon M, Sarcabal P, Willemot RM, Monsan P, Gajhede M. Amylosucrase, a glucan-synthesizing enzyme from the alpha-amylase family. J Biol Chem 2001; 276: 25273–25278
  • Skov LK, Mirza O, Henriksen A, Potocki DM, Remaud-Simeon M, Sarcabal P, Willemot RM, Monsan P, Gajhede M. Crystallization and preliminary X-ray studies of recombinant amylosucrase from Neisseria polysaccharea. Acta Crystallogr 2000; D56: 203–205
  • Skov LK, Mirza O, Sprogoe D, Dar I, Remaud-Simeon M, Albenne C, Monsan P, Gajhede M. Oligosaccharide and sucrose complexes of amylosucrase – Structural implications for the polymerase activity. J Biol Chem 2002; 277: 47741–47747
  • Sprogoe D, van den Broek LAM, Mirza O, Kastrup JS, Voragen AGJ, Gajhede M, Skov LK. Crystal structure of sucrose phosphorylase from Bifidobacterium adolescentis. Biochemistry 2004; 43: 1156–1162
  • van der Veen BA, Potocki-Veronese G, Albenne C, Joucla G, Monsan P, Remaud-Simeon M. Combinatorial engineering to enhance amylosucrase performance: construction, selection, and screening of variant libraries for increased activity. FEBS Lett 2004; 560: 91–97
  • Zhang DH, Li N, Lok SM, Zhang LH, Swaminathan K. Isomaltulose synthase (PalI) of Klebsiella sp LX3 – Crystal structure and implication of mechanism. J Biol Chem 2003; 278: 35428–35434

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