154
Views
12
CrossRef citations to date
0
Altmetric
Research Article

Expression of nitrile hydratase gene of mutant 4D strain of Rhodococcus rhodochrous PA 34 in Pichia pastoris

, &
Pages 19-26 | Received 03 Jul 2016, Accepted 01 Sep 2016, Published online: 31 Dec 2016

References

  • Bhalla TC, Aoshima M, Misawa S, Muamatsu R, Furuhashi K. 1995. Molecular cloning and sequencing of nitrilase gene of Rhodococcus rhodochrous PA-34. Acta Biotechnol 15:297–306.
  • Bhalla TC, Miura M, Wakamoto A, Ohba, Furuhashi K. 1992. Asymmetric hydrolysis of α-aminonitriles to optically active amino acids by a nitrilase of Rhodococcus rhodochrous PA-34. Appl Microbiol Biotechnol 37:184–190.
  • Cramp RA, Cowan DA. 1991. Molecular characterisation of a novel thermophilic nitrile hydratase. Biochem Biophys Acta (BBA)-Protein Struct Mol Enzymol 1431:249–260.
  • Dym O, Mevarech M, Sussman JL. 1995. Structural features that stabilize halophilic malate dehydrogenase from an archaebacterium. Science 267:1344–1346.
  • Frolow F, Harel M, Sussman JL, Mevarech M, Shoham M. 1996. Insights into protein adaptation to a saturated salt environment from the crystal structure of a halophilic 2Fe-2S ferredoxin. Nat Struct Biol 3:452–458.
  • Hann EC, Eisenberg A, Fager SK, Perkin NE, Gallagher FG, Cooper SM, Gavagan JE, Stieglitz B, Hennessey SM, Di-Cosimo R. 1999. 5-Cyanovaleramide production using immobilized Pseudomonas chlororaphis B 23. Bioorg Med Chem 7:2239–2245.
  • Ishibashi M, Arakawa T, Philo JS, Sakashita K, Yonezawa Y, Tokunaga H. 2002. Secondary and quaternary structural tension of the halopholic archaeon nucleoside diphosphate kinase under high and low salt conditions. FEMS Microbiol Lett 216:235–224.
  • Kim SH, Oriel P. 2000. Cloning and expression of the nitrile hydratase and amidase genes from Bacillus sp. BR449 into Escherichia coli. Enzyme Microb Technol 27:492–501.
  • Kobayashi M, Nishiyama M, Nagasawa T, Horinouchi S, Beppu T, Yamada H. 1991. Cloning, nucleotide sequence and expression in Escherichia coli of two cobalt-containing nitrile hydratase genes from Rhodococcus rhodochrous J1. Biochim Biophys Acta 1129:23–33.
  • Kobayashi M, Shimizu S. 2000. Metalloenzyme nitrile hydratase: structure, regulation, and application to biotechnology. Nat Biotechnol 16:733–736.
  • Li P, Anumanthan A, Gao XG, Ilangovan K, Suzara VV, Düzgünes N, Renugopalakrishnan V. 2007. Expression of recombinant proteins in Pichia pastoris. Appl Biochem Biotechnol 142:105–124.
  • Nojiri M, Yohda M, Odaka M, Matsushita Y, Tsujimura M, Yoshida T, Dohmae N, Takio K, Endo I. 1999. Functional expression of nitrile hydratase in Escherichia coli: requirement of a nitrile hydratase activator and post-translational modification of a ligand cysteine. J Biochem 125:696–704.
  • Prasad S, Raj J, Bhalla TC. 2004. Optimization of culture conditions for hyper production of nitrile hydratase of Rhodococcus rhodochrous PA-34. Indian J Microbiol 44:251–256.
  • Prasad S, Raj J, Bhalla TC. 2007. Bench scale conversion of 3-cyanopyidine to nicotinamide using resting cells of Rhodococcus rhodochrous PA-34. Indian J Microbiol 47:34–41.
  • Prasad S, Raj J, Bhalla TC. 2009. Purification of a hyperactive nitrile hydratase from resting cells of Rhodococcus rhodochrous PA-34. Indian J Microbiol 49:237–242.
  • Pratush A, Seth A, Bhalla TC. 2010. Generation of mutant of Rhodococcus rhodochrous PA-34 through chemical mutagenesis for hyperproduction of nitrile hydratase. Acta Microbiol Immunol Hung 57:135–146.
  • Pratush A, Seth A, Bhalla TC. 2011. Optimization of process parameters for conversion of 3-cyanopyridine to nicotinamide using resting cells of mutant 4D strain of Rhodococcus rhodochrous PA-34. Int J Bioautomat 15:151–158.
  • Precigou S, Goulas P, Duran R. 2000. Rapid and specific identification of nitrile hydratase (NHase)-encoding gene in soil samples by polymerase chain reaction. FEMS Microbiol Lett 204:155–161.
  • Raj J, Prasad S, Bhalla TC. 2006. Rhodococcus rhodochrous PA-34: a potential catalyst for acrylamide synthesis. Process Biochem 41:1359–1363.
  • Raj J, Seth A, Prasad S, Bhalla TC. 2007. Bioconversion of butyronitrile to butyramide using whole cells of Rhodococcus rhodochrous PA-34. Appl Microbiol Biotechnol 74:535–539.
  • Rzeznicka K, Schatzle S, Bottcher D, Klein J, Bornscheuer UT. 2009. Cloning and functional expression of a nitrile hydratase (NHase) from Rhodococcus equi TG328-2 in Escherichia coli, its purification and biochemical characterization. Appl Microbiol Biotechnol 85:1417–1425.
  • Silverman RB. 2002. The organic chemistry of enzyme catalyzed reactions. Enzymes and catalysis. New York: Academic.
  • Song L, Yuan HJ, Coffey L, Doran J, Wang MX, Qian S, Reilly CO. 2008. Efficient expression in E. coli of an enantioselective nitrile hydratase from Rhodococcus erythropolis. Biotechnol Lett 30:755–762.
  • Wu S, Fallon RD, Payne MS. 1997. Over-production of stereo selective nitrile hydratase from Pseudomonas putida 5B in Escherichia coli: activity requires a novel downstream protein. Appl Microbiol Biotechnol 48:704–708.
  • Yoshiyuki H, Yasuki F, Shigeyuki K, Wataru H, Kousaku M. 2002. Extremely simple, rapid and highly efficient transformation method for the yeast Saccharomyces cerevisiae using glutathione and early log phase cells. J Biosci Bioeng 94:166–171.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.