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Review Article

Nuclear Magnetic Resonance Spectroscopy for Quantitative Analysis: A Review for Its Application in the Chemical, Pharmaceutical and Medicinal Domains

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Pages 997-1011 | Published online: 09 Nov 2021

References

  • Jungnickel, J. L.; Forbes, J. W. Quantitative Measurement of Hydrogen Types by Integrated Nuclear Magnetic Resonance Intensities. Anal. Chem. 1963, 35, 938–942. DOI: 10.1021/ac60201a005.
  • Hollis, D. P. Quantitative Analysis of Aspirin, Phenacetin, and Caffeine Mixtures by Nuclear Magnetic Resonance Spectrometry. Anal. Chem. 1963, 35, 1682–1684. DOI: 10.1021/ac60204a043.
  • Steinhof, O.; Kibrik, É. J.; Scherr, G.; Hasse, H. Quantitative and Qualitative 1H, 13C, and 15N NMR Spectroscopic Investigation of the Urea-Formaldehyde Resin Synthesis. Magn. Reson. Chem. 2014, 52, 138–162. DOI: 10.1002/mrc.4044.
  • Pauli, G. F.; Gödecke, T.; Jaki, B. U.; Lankin, D. C. Quantitative 1H NMR. Development and Potential of an Analytical Method: An Update. J. Nat. Prod. 2012, 75, 834–851. DOI: 10.1021/np200993k.
  • Cerceau, C. I.; Barbosa, L. C. A.; Filomeno, C. A.; Alvarenga, E. S.; Demuner, A. J.; Fidencio, P. H. An Optimized and Validated (1)H NMR Method for the Quantification of α-Pinene in Essentials Oils. Talanta 2016, 150, 97–103. DOI: 10.1016/j.talanta.2015.10.087.
  • Branch, S. K. Guidelines from the International Conference on Harmonisation (ICH). J. Pharm. Biomed. Anal. 2005, 38, 798–805. DOI: 10.1016/j.jpba.2005.02.037.
  • United States Pharmacopoeia (USP) 32. The United States Pharmacopoeia Convention. Rockville, MD, 2009
  • The British Pharmacopeia British Pharmacopeia Commission; London, UK, 2013.
  • European Pharmacopoeia, 6th ed.; European Department for the Quality of Medicines: Strasbourg, France, 2009.
  • Giraudeau, P. Challenges and Perspectives in Quantitative NMR. Magn. Reson. Chem. 2017, 55, 61–69. DOI: 10.1002/mrc.4475.
  • Lauterbur, P. C. C13 Nuclear Magnetic Resonance Spectra. J. Chem. Phys. 1957, 26, 217–218. DOI: 10.1063/1.1743253.
  • Shoolery, J. N. Some Quantitative Applications of 13C NMR Spectroscopy. Prog. Nucl. Magn. Reson. Spectrosc. 1977, 11, 79–93. DOI: 10.1016/0079-6565(77)80003-4.
  • Jeener, J. Lecture Presented at Ampere International Summer School II, Basko Polje, Yugoslavia, 1971.
  • Alonso, J.; Arús, C.; Westler, W. M.; Markley, J. L. Two-Dimensional Correlated Spectroscopy (COSY) of Intact Frog Muscle: Spectral Pattern Characterization and Lactate Quantitation. Magn. Reson. Med. 1989, 11, 316–330. DOI: 10.1002/mrm.1910110306.
  • Webster, G. K.; Bell, R. G. Practical Approaches to Qualifying Laboratory Standard Reference Materials. Pharmac. Formulation Qual. 1999, 39–43.
  • Kashif, M.; Khalil, A.; Rahman, N. Pharmaceutical Industry and the Role of an Analyst. MACIJ. 2018, 2. DOI: 10.23880/MACIJ-16000120.
  • Holzgrabe, U. Quantitative NMR Spectroscopy in Pharmaceutical Applications. Prog. Nucl. Magn. Reson. Spectrosc. 2010, 57, 229–240. DOI: 10.1016/j.pnmrs.2010.05.001.
  • Franco, P. H. C.; Braga, S. F. P.; de Oliveira, R. B.; César, I. C. Purity Determination of a New Antifungal Drug Candidate Using Quantitative 1 H NMR Spectroscopy: Method Validation and Comparison of Calibration Approaches. Magn. Reson. Chem. 2020, 58, 97–105. DOI: 10.1002/mrc.4936.
  • Holzgrabe, U.; Malet-Martino, M. Analytical Challenges in Drug Counterfeiting and falsification-The NMR Approach. J. Pharm. Biomed. Anal. 2011, 55, 679–687. DOI: 10.1016/j.jpba.2010.12.017.
  • Ribeiro, M. V. D. M.; Boralle, N.; Pezza, H. R.; Pezza, L. Authenticity Assessment of Anabolic Androgenic Steroids in Counterfeit Drugs by 1H NMR. Anal. Methods 2018, 10, 1140–1150. DOI: 10.1039/C8AY00158H.
  • Peez, N.; Janiska, M. C.; Imhof, W. The First Application of Quantitative 1H NMR Spectroscopy as a Simple and Fast Method of Identification and Quantification of Microplastic Particles (PE, PET, and PS). Anal. Bioanal. Chem. 2019, 411, 823–833. DOI: 10.1007/s00216-018-1510-z.
  • Çiçek, S. S.; Esposito, T.; Girreser, U. Prediction of the Sweetening Effect of Siraitia Grosvenorii (Luo Han Guo) Fruits by Two-Dimensional Quantitative NMR. Food Chem. 2021, 335, 127622. DOI: 10.1016/j.foodchem.2020.127622.
  • Kemprai, P.; Mahanta, B. P.; Bora, P. K.; Das, D. J.; Boruah, J. L. H.; Saikia, S. P.; Haldar, S. A 1H NMR Spectroscopic Method for the Quantification of Propenylbenzenes in the Essential Oils: Evaluation of Key Odorants, Antioxidants and Post-Harvest Drying Techniques for Piper betle L . Food Chem. 2020, 331, 127278. DOI: 10.1016/j.foodchem.2020.127278.
  • Saito, T.; Yamano, M.; Nakayama, K.; Kawahara, S. Quantitative Analysis of Crosslinking Junctions of Vulcanized Natural Rubber through Rubber-State NMR Spectroscopy. Polym. Test 2021, 96, 107130. DOI: 10.1016/j.polymertesting.2021.107130.
  • Hussain, J. H.; Gilbert, N.; Costello, A.; Schofield, C. J.; Kemsley, E. K.; Sutcliffe, O. B.; Mewis, R. E. Quantification of MDMA in Seized Tablets Using Benchtop 1H NMR Spectroscopy in the Absence of Internal Standards. Forensic Chem. 2020, 20, 100263. DOI: 10.1016/j.forc.2020.100263.
  • Samuels, E. R.; Wang, T. Quantitative 1H NMR Analysis of a Difficult Drug Substance and Its Exo-Isomer as Hydrochloride Salts Using Alkaline Deuterated Methanol. J. Pharm. Biomed. Anal. 2020, 187, 113338. DOI: 10.1016/j.jpba.2020.113338.
  • Farhadi, F.; Nayebzadeh, N.; Badiee, A.; Arabsalmani, M.; Hatamipour, M.; Iranshahi, M. A Validated 1H-NMR Method for Quantitative Analysis of DOTAP Lipid in Nanoliposomes Containing Soluble Leishmania Antigen. J. Pharm. Biomed. Anal. 2021, 194, 113809. DOI: 10.1016/j.jpba.2020.113809.
  • Blunder, M.; Orthaber, A.; Bauer, R.; Bucar, F.; Kunert, O. Efficient Identification of Flavones, Flavanones and Their Glycosides in Routine Analysis via Off-Line Combination of Sensitive NMR and HPLC Experiments . Food Chem. 2017, 218, 600–609. DOI: 10.1016/j.foodchem.2016.09.077.
  • Bertelli, D.; Brighenti, V.; Marchetti, L.; Reik, A.; Pellati, F. Nuclear Magnetic Resonance and High-Performance Liquid Chromatography Techniques for the Characterization of Bioactive Compounds from Humulus Lupulus L. (Hop). Anal. Bioanal. Chem. 2018, 410, 3521–3531. DOI: 10.1007/s00216-018-0851-y.
  • Liu, N. Q.; Choi, Y. H.; Verpoorte, R.; Van der Kooy, F. Comparative Quantitative Analysis of Artemisinin by Chromatography and qNMR. Phytochem. Anal. 2010, 21, 451–456. DOI: 10.1002/pca.1217.
  • Spelta, F.; Liverani, L.; Peluso, A.; Marinozzi, M.; Urso, E.; Guerrini, M.; Naggi, A. SAX-HPLC and HSQC NMR Spectroscopy: Orthogonal Methods for Characterizing Heparin Batches Composition. Front Med. (Lausanne). 2019, 6, 78. DOI: 10.3389/fmed.2019.00078.
  • Yu, P.; Li, Q.; Feng, Y.; Chen, Y.; Ma, S.; Ding, X. Quantitative Analysis of Flavonoids in Glycyrrhiza Uralensis Fisch by 1H-qNMR. J. Anal. Methods Chem. 2021, 2021, 6655572. DOI: 10.1155/2021/6655572.
  • Sun, S.; Jin, M.; Zhou, X.; Ni, J.; Jin, X.; Liu, H.; Wang, Y. The Application of quantitative1H-NMR for the Determination of Orlistat in Tablets. Molecules 2017, 22, 1517. DOI: 10.3390/molecules22091517.
  • Yang, Q.; Qiu, H.; Guo, W.; Wang, D.; Zhou, X.; Xue, D.; Zhang, J.; Wu, S.; Wang, Y. Quantitative 1H-NMR Method for the Determination of Tadalafil in Bulk Drugs and Its Tablets. Molecules 2015, 20, 12114–12124. DOI: 10.3390/molecules200712114.
  • Dong, J. W.; Li, X. J.; Cai, L.; Shi, J. Y.; Li, Y. F.; Yang, C.; Li, Z. L. Simultaneous Determination of Alkaloids Dicentrine and Sinomenine in Stephania Epigeae by 1H NMR Spectroscopy. J. Pharm. Biomed. Anal. 2018, 160, 330–335. DOI: 10.1016/j.jpba.2018.08.007.
  • Qin, L.; Wang, X.; Lu, D. Quantitative Determination and Validation of Topiramate and Its Tablet Formulation by 1 H-NMR Spectroscopy. Anal. Methods 2019, 11, 661–668. DOI: 10.1039/C8AY02316F.
  • Nasr, J. J.; Shalan, S. Validated 1H and 19F Nuclear Magnetic Resonance for the Quantitative Determination of the Hepatitis C Antiviral Drugs Sofosbuvir, Ledipasvir, and Daclatasvir in Tablet Dosage Forms. Microchem. J 2020, 152, 104437. DOI: 10.1016/j.microc.2019.104437.
  • Paniagua-Vega, D.; Cavazos-Rocha, N.; Huerta-Heredia, A. A.; Parra-Naranjo, A.; Rivas-Galindo, V. M.; Waksman, N.; Saucedo, A. L. A Validated NMR Method for the Quantitative Determination of Rebaudioside a in Commercial Sweeteners. J. Food. Compos. Anal. 2019, 79, 134–142. DOI: 10.1016/j.jfca.2019.02.009.
  • Wang, C.; Zhang, P.; Zhang, X.; Yu, M.; Lv, Z. 1H NMR Quantification of Polydatin and Emodin in Huganning, a Chinese Patent Herbal Medicine. Rev. Bras. Farmacogn. 2020, 30, 28–33. DOI: 10.1007/s43450-020-00031-7.
  • Owczarek, A.; Kłys, A.; Olszewska, M. A. A Validated 1H qNMR Method for Direct and Simultaneous Quantification of Esculin, Fraxin and (–)-Epicatechin in Hippocastani Cortex. Talanta 2019, 192, 263–269. DOI: 10.1016/j.talanta.2018.09.036.
  • Cheilari, A.; Sturm, S.; Intelmann, D.; Seger, C.; Stuppner, H. Head-to-Head Comparison of Ultra-High-Performance Liquid Chromatography with Diode Array Detection versus Quantitative Nuclear Magnetic Resonance for the Quantitative Analysis of the Silymarin Complex in Silybum Marianum Fruit Extracts. J. Agric. Food Chem. 2016, 64, 1618–1626. DOI: 10.1021/acs.jafc.5b05494.
  • Napolitano, J. G.; Gödecke, T.; Lankin, D. C.; Jaki, B. U.; McAlpine, J. B.; Chen, S. N.; Pauli, G. F. Orthogonal Analytical Methods for Botanical Standardization: Determination of Green Tea Catechins by qNMR and LC-MS/MS. J. Pharm. Biomed. Anal. 2014, 93, 59–67. DOI: 10.1016/j.jpba.2013.06.017.
  • Bouatra, S.; Aziat, F.; Mandal, R.; Guo, A. C.; Wilson, M. R.; Knox, C.; Bjorndahl, T. C.; Krishnamurthy, R.; Saleem, F.; Liu, P.; et al. The Human Urine Metabolome. PLoS One. 2013, 8, e73076. DOI: 10.1371/journal.pone.0073076.
  • Djukovic, D.; Raftery, D.; Gowda, N. Chapter 16 - Mass Spectrometry and NMR Spectroscopy Based Quantitative Metabolomics; Academic Press: Boston, MA, 2020; pp 289–311. DOI: 10.1016/B978-0-12-818607-7.00016-5.
  • Mohn, T.; Cutting, B.; Ernst, B.; Hamburger, M. Extraction and Analysis of Intact glucosinolates-A Validated Pressurized Liquid Extraction/Liquid Chromatography-Mass Spectrometry Protocol for Isatis Tinctoria, and Qualitative Analysis of Other Cruciferous Plants. J. Chromatogr. A. 2007, 1166, 142–151. DOI: 10.1016/j.chroma.2007.08.028.
  • Hatzakis, E. Nuclear Magnetic Resonance (NMR) Spectroscopy in Food Science: A Comprehensive Review. Compr. Rev. Food Sci. Food Saf. 2019, 18, 189–220. DOI: 10.1111/1541-4337.12408.
  • Gathungu, R. M.; Kautz, R.; Kristal, B. S.; Bird, S. S.; Vouros, P. The Integration of LC-MS and NMR for the Analysis of Low Molecular Weight Trace Analytes in Complex Matrices. Mass Spectrom. Rev. 2020, 39, 35–54. DOI: 10.1002/mas.21575.
  • Quinn, C. M.; Wang, M.; Polenova, T. NMR of Macromolecular Assemblies and Machines at 1 GHz and beyond: new Transformative Opportunities for Molecular Structural Biology. Protein NMR 2018, 1688, 1–35. DOI: 10.1007/978-1-4939-7386-6_1.
  • Installation of World’s First 1.2 GHz NMR System Enables Novel Functional Structural Biology Research.
  • Gan, Z.; Hung, I.; Wang, X.; Paulino, J.; Wu, G.; Litvak, I. M.; Gor'kov, P. L.; Brey, W. W.; Lendi, P.; Schiano, J. L.; et al. NMR Spectroscopy up to 35.2T Using a Series-Connected Hybrid Magnet. J. Magn. Reson. 2017, 284, 125–136. DOI: 10.1016/j.jmr.2017.08.007.
  • Rahman, A.; Choudhary, M. I.; Wahab, A. Solving Problems with NMR Spectroscopy, 2nd ed.; Elsevier Academic Press: London, 2016.
  • Singh, S.; Roy, R. The application of absolute quantitative 1H NMR spectroscopy in drug discovery and development. Expert Opin. Drug Discov. 2016, 11, 695–706. DOI: 10.1080/17460441.2016.1189899.
  • Michaleas, S.; Antoniadou-Vyza, E. A New Approach to Quantitative NMR: Fluoroquinolones Analysis by Evaluating the Chemical Shift Displacements. J. Pharm. Biomed. Anal. 2006, 42, 405–410. DOI: 10.1016/j.jpba.2006.04.016.
  • Zheng, G.; Price, W. S. Solvent Signal Suppression in NMR. Prog. Nucl. Magn. Reson. Spectrosc. 2010, 56, 267–288. DOI: 10.1016/j.pnmrs.2010.01.001.
  • Griffiths, L.; Irving, A. M. Assay by Nuclear Magnetic Resonance Spectroscopy: Quantification Limits. Analyst 1998, 123, 1061–1068. DOI: 10.1039/a800625c.
  • Crocoli, L. C.; Ortiz, R. S.; Moura, S. Development and Validation of a qNMR Method for Analyses of Legal and Illegal Formulations of Glyphosate. Anal. Methods 2019, 11, 4052–4059. DOI: 10.1039/C9AY00673G.
  • Webster, G. K.; Kumar, S. Expanding the Analytical Toolbox: Pharmaceutical Application of Quantitative NMR. Anal. Chem. 2014, 86, 11474–11480. DOI: 10.1021/ac502871w.
  • Jadeja, Y.; Chomal, B.; Patel, M.; Jebaliya, H.; Khunt, R.; Shah, A. Method Development and Validation: quantitation of Telmisartan Bulk Drug and Its Tablet Formulation by 1 H NMR Spectroscopy. Magn. Reson. Chem. 2017, 55, 634–638. DOI: 10.1002/mrc.4565.
  • Khalil, A.; Kashif, M. Use of Box-Behnken Design for the Nuclear Magnetic Resonance Study of Molecular Complex of Anticonvulsant Drug with N,N-Dimethylformamide and Its Application in Quantitative Analysis. J. Mol. Liq. 2021, 326, 115308. DOI: 10.1016/j.molliq.2021.115308.
  • Pauli, G. F.; Chen, S. N.; Simmler, C.; Lankin, D. C.; Gödecke, T.; Jaki, B. U.; Friesen, J. B.; McAlpine, J. B.; Napolitano, J. G. Importance of Purity Evaluation and the Potential of Quantitative 1H NMR as a Purity Assay. J. Med. Chem. 2014, 57, 9220–9231. DOI: 10.1021/jm500734a.
  • Pharmacopoeia, U. S. United States Pharmacopoeia Heparin Sodium Stage 3 Monograph; US Pharmacopoeia: Rockville, 2012.
  • USP XXIII-NF18, US Pharmacopeial Convention, Inc.: Mack: Easton, PA, 1995, vol. 107; pp 1681–1696.
  • Hanna, G. M. Nuclear Magnetic Resonance Spectroscopic Determination of Dicyclomine Hydrochloride in Tablet, Capsule, and Injection Dosage Forms. J. Assoc. Off. Anal. Chem. 1984, 67, 222–224. DOI: 10.1093/jaoac/67.2.222.
  • Ishihara, S.; Labuta, J.; Futera, Z.; Mori, S.; Sato, H.; Ariga, K.; Hill, J. P. NMR Spectroscopic Determination of Enantiomeric Excess Using Small Prochiral Molecules. J. Phys. Chem. B. 2018, 122, 5114–5120. DOI: 10.1021/acs.jpcb.8b03684.
  • Miao, Z.; Jin, M.; Liu, X.; Guo, W.; Jin, X.; Liu, H.; Wang, Y. The Application of HPLC and Microprobe NMR Spectroscopy in the Identification of Metabolites in Complex Biological Matrices. Anal. Bioanal. Chem. 2015, 407, 3405–3416. DOI: 10.1007/s00216-015-8556-y.
  • Maschmeyer, T.; Prieto, P. L.; Grunert, S.; Hein, J. E. Exploration of Continuous-Flow Benchtop NMR Acquisition Parameters and Considerations for Reaction Monitoring. Magn. Reson. Chem. 2020, 58, 1234–1248. DOI: 10.1002/mrc.5094.
  • Dunn, A. L.; Landis, C. R. Progress toward Reaction Monitoring at Variable Temperatures: A New Stopped-Flow NMR Probe Design. Magn. Reson. Chem. 2017, 55, 329–336. DOI: 10.1002/mrc.4538.
  • Kumar, V.; Dwivedi, D. K.; Jagannathan, N. R. High-Resolution NMR Spectroscopy of Human Body Fluids and Tissues in Relation to Prostate Cancer. NMR Biomed. 2014, 27, 80–89. DOI: 10.1002/nbm.2979.
  • Everett, J. R.; Jennings, K. R.; Woodnutt, G.; Buckingham, M. J. Spin-Echo 1H N.M.R. spectroscopy: A New Method for Studying Penicillin Metabolism. J. Chem. Soc, Chem. Commun. 1984, 894–895. DOI: 10.1039/c39840000894.
  • Opella, S. J.; Marassi, F. M. Applications of NMR to Membrane Proteins. Arch. Biochem. Biophys. 2017, 628, 92–101. DOI: 10.1016/j.abb.2017.05.011.
  • Mertens, H. D. T.; Svergun, D. I. Combining NMR and Small Angle X-Ray Scattering for the Study of Biomolecular Structure and Dynamics. Arch. Biochem. Biophys. 2017, 628, 33–41. DOI: 10.1016/j.abb.2017.05.005.
  • Gawande, V. T.; Bothara, K. G.; Marathe, A. M. Stress Studies and Identification of Degradation Products of Cephalexin Using LC–PDA and LC–MS/MS. Chromatographia 2017, 80, 1545–1552. DOI: 10.1007/s10337-017-3385-0.
  • Shah, U.; Kavad, M.; Raval, M. Development and Validation of Stability-Indicating RP-HPLC Method for Estimation of Pamabrom in Tablets. Indian J. Pharm. Sci. 2014, 76, 198–202.
  • Kumar, K. V.; Sharma, H.; Peraman, R.; Pudipatla, S. S. A Stability-Indicating RP-HPLC-UV Method for Determination of Fidaxomicin and Its Hydrolytic Degradation Products. J. Iran. Chem. Soc 2021. DOI: 10.1007/s13738-021-02343-4.
  • Miura, Y. NMR Studies on the Conformation, Stability and Dynamics of Alamethicin in Methanol. Eur. Biophys. J. 2020, 49, 113–124. DOI: 10.1007/s00249-019-01418-8.
  • Moraes, A.; Ackerbauer, D.; Kostadinova, M.; Bublin, M.; Oliveira, G. A.; Ferreira, F.; Almeida, F. C. L.; Breiteneder, H.; Valente, A. P. Solution and High-Pressure NMR Studies of the Structure, Dynamics and Stability of the Cross-Reactive Allergenic Cod Parvalbumin Gad m 1. World Allergy Organ J. 2015, 8, A180. DOI: 10.1186/1939-4551-8-S1-A180.
  • Dračínský, M.; Krečmerová, M.; Holý, A. Study of Chemical Stability of Antivirally Active 5-Azacytosine Acyclic Nucleoside Phosphonates Using NMR Spectroscopy. Bioorg. Med. Chem. 2008, 16, 6778–6782. DOI: 10.1016/j.bmc.2008.05.058.
  • Salman, D.; Peron, J. M. R.; Goronga, T.; Barton, S.; Swinden, J.; Nabhani-Gebara, S. H. & NMR-Based Forced Degradation Studies of Ifosfamide: The Potential of NMR in Stability Studies. Ann. Pharm. Fr. 2016, 74, 119–128. DOI: 10.1016/j.pharma.2015.09.004.s.
  • Sahu, A.; Balhara, A.; Raju, N.; Kumar, B. K.; Sharma, P.; Singh, D. K.; Singh, S. Characterization of Degradation Products of Celiprolol Hydrochloride Using Hyphenated Mass and NMR Techniques. J. Pharm. Biomed. Anal. 2021, 197, 113953. DOI: 10.1016/j.jpba.2021.113953.
  • Qi, W.; Orgel, S.; Francon, A.; Randolph, T. W.; Carpenter, J. F. Urea Improves Stability of Inactivated Polio Vaccine Serotype 3 during Lyophilization and Storage in Dried Formulations. J. Pharm. Sci. 2018, 107, 2070–2078. DOI: 10.1016/j.xphs.2018.04.019.
  • Liu, L.; Mo, H.; Wei, S.; Raftery, D. Quantitative Analysis of Urea in Human Urine and Serum by 1H Nuclear Magnetic Resonance. Analyst 2012, 137, 595–600. DOI: 10.1039/c2an15780b.
  • Mamone, S.; Rezaei-Ghaleh, N.; Opazo, F.; Griesinger, C.; Glöggler, S. Singlet-Filtered NMR Spectroscopy. Sci. Adv. 2020, 8, 1–7. DOI: 10.1126/sciadv.aaz1955.
  • Song, Z.; Wang, H.; Yin, X.; Deng, P.; Jiang, W. Application of NMR Metabolomics to Search for Human Disease Biomarkers in Blood. Clin. Chem. Lab. Med. 2019, 57, 417–441. DOI: 10.1515/cclm-2018-0380.
  • Kumar, D.; Gupta, A.; Nath, K. NMR-Based Metabolomics of Prostate Cancer: A Protagonist in Clinical Diagnostics. Expert Rev. Mol. Diagn. 2016, 16, 651–661. DOI: 10.1586/14737159.2016.1164037.
  • DeFeo, E. M.; Wu, C. L.; McDougal, W. S.; Cheng, L. L. A Decade in Prostate Cancer: From NMR to Metabolomics. Nat. Rev. Urol. 2011, 8, 301–311. DOI: 10.1038/nrurol.2011.53.
  • Hu, J. M.; Sun, H. T. Serum Proton NMR Metabolomics Analysis of Human Lung Cancer following Microwave Ablation. Radiat. Oncol. 2018, 13, 1–10. DOI: 10.1186/s13014-018-0982-5.
  • Pandey, R.; Caflisch, L.; Lodi, A.; Brenner, A. J.; Tiziani, S. Metabolomic Signature of Brain Cancer. Mol. Carcinog. 2017, 56, 2355–2371. DOI: 10.1002/mc.22694.Metabolomic.
  • Junior, G. C. D. S.; Pereira, C. M.; Da Silva Fidalgo, T. K.; Valente, A. P. Saliva NMR-Based Metabolomics in the War against COVID-19. Anal. Chem. 2020, 92, 15688–15692. DOI: 10.1021/acs.analchem.0c04679.
  • Schoenle, M. V.; Li, Y.; Yuan, M.; Clarkson, M. W.; Wilson, I. A.; Peti, W.; Page, R. NMR Based SARS-CoV-2 Antibody Screening. J. Am. Chem. Soc. 2021, 143, 7930–7935. DOI: 10.1021/jacs.1c03945.
  • Covid-19 NMR project. https://covid19-nmr.de.

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