High-sensitivity cardiac troponin assays: From improved analytical performance to enhanced risk stratification

References

• Aakre KM, Røraas T, Petersen PH, Svarstad E, Sellevoll H, Skadberg Ø, Sæle K, Sandberg S. 2014. Weekly and 90-minute biological variations in cardiac troponin T and cardiac troponin I in hemodialysis patients and healthy controls. Clin Chem. 60:838–847.
   PubMed Web of Science ®Google Scholar
• Adams JE III, Bodor GS, Dávila-Román VG. 1993. Cardiac troponin I. A marker with high specificity for cardiac injury. Circulation. 88:101–106.
   PubMed Web of Science ®Google Scholar
• Agewall S, Giannitsis E, Jernberg T, Katus H. 2011. Troponin elevation in coronary vs. non-coronary disease. Eur Heart J. 32:404–411.
   PubMed Web of Science ®Google Scholar
• Al-Saleh A, Alazzoni A, Al Shalash S, Ye C, Mbuagbaw L, Thabane L, Jolly SS. 2014. Performance of the high-sensitivity troponin assay in diagnosing acute myocardial infarction: systematic review and meta-analysis. CMAJ Open. 2:E199–E207.
   Google Scholar
• Analytical characteristics of commercial cardiac troponin I and T assays declared by the manufacturer. 2016. Available from: http://www.ifcc.org/media/276661/IFCC%20Troponin%20Tables%20ng_L%20DRAFT%20Update%20NOVEMBER%202014.pdf [last accessed 10 Dec 2016].
   Google Scholar
• Apple FS. 2009. A new season for cardiac troponin assays: it’s time to keep a scorecard. Clin Chem. 55:1303–1306.
   PubMed Web of Science ®Google Scholar
• Apple FS. 2012. Counterpoint: standardization of cardiac troponin I assays will not occur in my lifetime. Clin Chem. 58:169–171.
   PubMed Web of Science ®Google Scholar
• Apple F, Sandoval Y. 2016. Implementing high sensitivity cardiac troponin assays. Available from: https://www.aacc.org/publications/cln/articles/2015/december/implementing-high-sensitivity-troponin assays [last accessed 10 Dec 2016].
   Google Scholar
• Apple FS, Collinson PO. 2012. Analytical characteristics of high-sensitivity cardiac troponin assays. Clin Chem. 58:54–61.
   PubMed Web of Science ®Google Scholar
• Apple FS, Hollander J, Wu AH, Jaffe AS. 2014. Improving the 510(k) FDA process for cardiac troponin assays: in search of common ground. Clin Chem. 60:1273–1275.
   PubMed Web of Science ®Google Scholar
• Apple FS, Jaffe AS, Collinson P, Mockel M, Ordonez-Llanos J, Lindahl B, Hollander J, Plebani M, Than M, Chan MHM, et al. 2015. IFCC educational materials on selected analytical and clinical applications of high sensitivity cardiac troponin assays. Clin Biochem. 48:201–203.
   PubMed Web of Science ®Google Scholar
• Apple FS, Ler R, Murakami MM. 2012. Determination of 19 cardiac troponin I and T assay 99th percentile values from a common presumably healthy population. Clin Chem. 58:1574–1581.
   PubMed Web of Science ®Google Scholar
• Apple FS, Sandoval Y, Jaffe AS, Ordonez-Llanos J. 2017. Cardiac troponin assays: guide to understanding analytical characteristics and their impact on clinical care. Clin Chem. 63:73–81.
   PubMed Web of Science ®Google Scholar
• Aw TC, Phua SK, Tan SP. 2013. Measurement of cardiac troponin I in serum with a new high-sensitivity assay in a large multi-ethnic Asian cohort and the impact of gender. Clin Chim Acta. 422:26–28.
   PubMed Web of Science ®Google Scholar
• Blankenberg S, Salomaa V, Makarova N, Ojeda F, Wild P, Lackner KJ, Jørgensen T, Thorand B, Peters A, Nauck M, et al. 2016. Troponin I and cardiovascular risk prediction in the general population: the BiomarCaRE consortium. Eur Heart J. 37:2428.
   PubMed Web of Science ®Google Scholar
• Bohula May EA, Bonaca MP, Jarolim P, Antman EM, Braunwald E, Giugliano RP, Newby LK, Sabatine MS, Morrow DA. 2014. Prognostic performance of a high-sensitivity cardiac troponin I assay in patients with non-ST-elevation acute coronary syndrome. Clin Chem. 60:158–164.
   PubMed Web of Science ®Google Scholar
• Braga F, Panteghini M. 2016. Generation of data on within-subject biological variation in laboratory medicine: an update. Crit Rev Clin Lab Sci. 53:313–325.
   PubMed Web of Science ®Google Scholar
• Cardiac troponin I. 2016. Available from: https://www.hytest.fi/sites/52cd5c487653512f63000004/content_entry52cd6295765351528d000020/541aa02a7653519314002a6a/files/Troponin_Booklet.pdf [last accessed 10 Dec 2016].
   Google Scholar
• Carlton E, Greenslade J, Cullen L, Body R, Than M, Pickering JW, Aldous S, Carley S, Hammett C, Kendall J, et al. 2016. Evaluation of high-sensitivity cardiac troponin I levels in patients with suspected acute coronary syndrome. JAMA Cardiol. 1:405–412.
   PubMed Web of Science ®Google Scholar
• Chaitman BR. 2015. Is the 99th percentile the optimal reference limit to diagnose myocardial infarction with high-sensitivity cardiac troponin assays in patients with chronic kidney disease? Circulation. 131:2029–2031.
   PubMed Web of Science ®Google Scholar
• Chenevier-Gobeaux C, Bailleul S, Mzabi A, Blanc MC, Lefevre G. 2013. Upper reference limits of high-sensitivity cardiac troponin T in a general population: comparison with those of sensitive cardiac troponin I. Clin Lab. 59:333–336.
   PubMed Web of Science ®Google Scholar
• Christenson RH, Duh SH, Apple FS, Bodor GS, Bunk DM, Dalluge J, Panteghini M, Potter JD, Welch MJ, Wu AH, et al. 2001. Standardization of cardiac troponin I assays: round robin of ten candidate reference materials. Clin Chem. 47:431–437.
   PubMed Web of Science ®Google Scholar
• Christenson RH, Duh SH, Apple FS, Bodor GS, Bunk DM, Panteghini M, Welch MJ, Wu AH, Kahn SE. 2006. Toward standardization of cardiac troponin I measurements. Part II: assessing commutability of candidate reference materials and harmonization of cardiac troponin I assays. Clin Chem. 52:1685–1692.
   PubMed Web of Science ®Google Scholar
• Christenson RH. 2007. National academy of clinical biochemistry laboratory medicine practice guidelines for utilization of biochemical markers in acute coronary syndromes and heart failure. Clin Chem. 53:545–546.
   PubMed Web of Science ®Google Scholar
• Clinical Laboratory Standards Institute. 2010. Defining, establishing and verifying reference intervals in the clinical laboratory; approved guideline. 3rd ed. EP28-A3c. Wayne (PA): Clinical and Laboratory Standards Institute.
   Google Scholar
• Collinson PO, Gaze D, Goodacre S. 2015. The clinical and diagnostic performance characteristics of the high sensitivity Abbott cardiac troponin I assay. Clin Biochem. 48:275–281.
   PubMed Web of Science ®Google Scholar
• Collinson PO, Heung YM, Gaze D, Boa F, Senior R, Christenson R, Apple FS. 2012. Influence of population selection on the 99th percentile reference value for cardiac troponin assays. Clin Chem. 58:219–225.
   PubMed Web of Science ®Google Scholar
• Conrad MJ, Jarolim P. 2014. Cardiac troponins and high-sensitivity cardiac troponin assays. Clin Lab Med. 34:59–73.
   PubMed Web of Science ®Google Scholar
• Corte Z, García C, Venta R. 2015. Biological variation of cardiac troponin T in patients with end-stage renal disease and in healthy individuals. Ann Clin Biochem. 52:53–60.
   PubMed Web of Science ®Google Scholar
• Costa, FM, Ferreira J, Aguiar C, Dores H, Figueira J, Mendes M. 2012. Impact of ESC/ACCF/AHA/WHF universal definition of myocardial infarction on mortality at 10 years. Eur Heart J. 33:2544–2550.
   PubMed Web of Science ®Google Scholar
• Cullen L, Mueller C, Parsonage WA, Wildi K, Greenslade JH, Twerenbold R, Aldous S, Meller B, Tate JR, Reichlin T, et al. 2013. Validation of high-sensitivity troponin I in a 2-hour diagnostic strategy to assess 30-day outcomes in emergency department patients with possible acute coronary syndrome. J Am Coll Cardiol. 62:1242–1249.
   PubMed Web of Science ®Google Scholar
• Cummins B, Auckland ML, Cummins P. 1987. Cardiac-specific troponin-I radioimmunoassay in the diagnosis of acute myocardial infarction. Am Heart J. 113:1333–1344.
   PubMed Web of Science ®Google Scholar
• Dati F, Panteghini M, Apple FS, Christenson RH, Mair J, Wu AH. 1999. Proposals from IFCC Committee on Standardization of Markers of Cardiac Damage (C-SMCD): strategies and concepts on standardization of cardiac marker assays. Scand J Clin Lab Invest. 59:113–123.
   Web of Science ®Google Scholar
• de Lemos JA, Drazner MH, Omland T, Ayers CR, Khera A, Rohatgi A, Hashim I, Berry JD, Das SR, Morrow DA, et al. 2010. Association of troponin T detected with a highly sensitive assay and cardiac structure and mortality risk in the general population. JAMA. 304:2503–2512.
   PubMed Web of Science ®Google Scholar
• deFilippi CR, de Lemos JA, Christenson RH, Gottdiener JS, Kop WJ, Zhan M, Seliger SL. 2010. Association of serial measures of cardiac troponin T using a sensitive assay with incident heart failure and cardiovascular mortality in older adults. JAMA. 304:2494–2502.
   PubMed Web of Science ®Google Scholar
• Di Somma S, Zampini G, Vetrone F, Soto-Ruiz KM, Magrini L, Cardelli P, Ronc C, Maisel A, Peacock FW. 2014. Opinion paper on utility of point-of-care biomarkers in the emergency department pathways decision making. Clin Chem Lab Med. 52:1401–1407.
   PubMed Web of Science ®Google Scholar
• Dupuy AM, Lozano C, Badiou S, Bargnoux AS, Kuster N, Cristol JP. 2013. Biological variability of hs-cardiac troponin T on the Roche Cobas 8000/e602® immunoanalyzer. Clin Chim Acta. 425:62–63.
   PubMed Web of Science ®Google Scholar
• Eggers KM, Johnston N, Lind L, Venge P, Lindahl B. 2015. Cardiac troponin I levels in an elderly population from the community: the implications of sex. Clin Biochem. 48:751–756.
   PubMed Web of Science ®Google Scholar
• Eggers KM, Lind L, Venge P, Lindahl B. 2013. Factors influencing the 99th percentile of cardiac troponin I evaluated in community-dwelling individuals at 70 and 75 years of age. Clin Chem. 59:1068–1073.
   PubMed Web of Science ®Google Scholar
• Eggers KM, Lindahl B, Melki D, Jernberg T. 2016. Consequences of implementing a cardiac troponin assay with improved sensitivity at Swedish coronary care units: an analysis from the SWEDEHEART registry. Eur Heart J. 37:2417–2424.
   PubMed Web of Science ®Google Scholar
• Executive summary: Scientific Division 56th Meeting. 2015 Nov 20–21. Milan, Italy. Available from: http://www.ifcc.org/media/410758/IFCC_SD_Minutes_56_Milan_summary.pdf
   Google Scholar
• Fahim MA, Hayen AD, Horvath AR, Dimeski G, Coburn A, Tan K-S, Johnson DW, Craig JC, Campbell SB, Hawley CM, et al. 2015. Biological variation of high sensitivity cardiac troponin-T in stable dialysis patients: implications for clinical practice. Clin Chem Lab Med. 53:715–722.
   PubMed Web of Science ®Google Scholar
• Feng H-Z, Biesiadecki BJ, Yu Z-B, Hossain MM, Jin J-P. 2008. Restricted N-terminal truncation of cardiac troponin T: a novel mechanism for functional adaptation to energetic crisis. J Physiol (Lond). 586:3537–3550.
   Google Scholar
• Frankenstein L, Wu AHB, Hallermayer K, Wians FH, Giannitsis E, Katus HA. 2011. Biological variation and reference change value of high-sensitivity troponin T in healthy individuals during short and intermediate follow-up periods. Clin Chem. 57:1068–1071.
   PubMed Web of Science ®Google Scholar
• Franzini M, Lorenzoni V, Masotti S, Prontera C, Chiappino D, Latta DD, Daves M, Deluggi I, Zuin M, Ferrigno L, et al. 2015. The calculation of the cardiac troponin T 99th percentile of the reference population is affected by age, gender, and population selection: a multicenter study in Italy. Clin Chim Acta. 438:376–381.
   PubMed Web of Science ®Google Scholar
• Fraser CG. 2016. Interpretation of differences in serial troponin results. Available from: https://www.westgard.com/troponin-interpretations.htm
   Google Scholar
• Gaggin HK, Dang PV, Do LD, deFilippi CR, Christenson RH, Lewandrowski EL, Lewandrowski KB, Truong BQ, Pham VQ, Vu VH, et al. 2014. Reference interval evaluation of high-sensitivity troponin T and N-terminal B-type natriuretic peptide in Vietnam and the US: the North South East West Trial. Clin Chem. 60:758–764.
   PubMed Web of Science ®Google Scholar
• Giannitsis E, Katus HA. 2013. Cardiac troponin level elevations not related to acute coronary syndromes. Nat Rev Cardiol. 10:623–634.
   PubMed Web of Science ®Google Scholar
• Giannitsis E, Kurz K, Hallermayer K, Jarausch J, Jaffe AS, Katus HA. 2010. Analytical validation of a high-sensitivity cardiac troponin T assay. Clin Chem. 56:254–261.
   PubMed Web of Science ®Google Scholar
• Giannoni A, Giovannini S, Clerico A. 2009. Measurement of circulating concentrations of cardiac troponin I and T in healthy subjects: a tool for monitoring myocardial tissue renewal? Clin Chem Lab Med. 47:1167–1177.
   PubMed Web of Science ®Google Scholar
• Goldberg J, Iriate B, Prostko J. 2011. Short and long-term biological variation of troponin-I determined using the Architect STAT high sensitive troponin-I assay. Clin Chem. 57:A97.
   Google Scholar
• Gomes AV, Potter JD, Szczesna-Cordary D. 2002. The role of troponins in muscle contraction. IUBMB Life. 54:323–333.
   PubMed Web of Science ®Google Scholar
• Gore MO, Seliger SL, Defilippi CR, Nambi V, Christenson RH, Hashim IA, Hoogeveen RC, Ayers CR, Sun W, McGuire DK, et al. 2014. Age- and sex-dependent upper reference limits for the high-sensitivity cardiac troponin T assay. J Am Coll Cardiol. 63:1441–1448.
   PubMed Web of Science ®Google Scholar
• Greene DN, Leong TK, Collinson PO, Kamer SM, Huang K, Lorey TS, Go AS. 2015. Age, sex, and racial influences on the Beckman Coulter AccuTnI +3 99th percentile. Clin Chim Acta. 444:149–153.
   PubMed Web of Science ®Google Scholar
• Haaf P, Reichlin T, Twerenbold R, Hoeller R, Rubini Gimenez M, Zellweger C, Moehring B, Fischer C, Meller B, Wildi K, et al. 2014. Risk stratification in patients with acute chest pain using three high-sensitivity cardiac troponin assays. Eur Heart J. 35:365–375.
   PubMed Web of Science ®Google Scholar
• Hamm CW, Bassand JP, Agewall S, Bax J, Boersma E, Bueno H, Caso P, Dudek D, Gielen S, Huber K, Ohman M, Petrie MC, Sonntag F, Uva MS, Storey RF, Wijns W, Zahger D; ESC Committee for Practice Guidelines. 2011. ESC guidelines for the management of acute coronary syndromes in patients presenting without persistent ST-segment elevation: The Task Force for the management of acute coronary syndromes (ACS) in patients presenting without persistent ST-segment elevation of the European Society of Cardiology (ESC). Eur Heart J. 32:2999–3054.
   PubMed Web of Science ®Google Scholar
• Hamm CW, Giannitsis E, Katus HA. 2002. Cardiac troponin elevations in patients without acute coronary syndrome. Circulation. 106:2871–2872.
   PubMed Web of Science ®Google Scholar
• Hausner EA. 2015. Cardiac muscle and the troponins. In: Gowraganahalli J, Pitchai B, Khin MU, editors. Pathophysiology and pharmacotherapy of cardiovascular disease. Switzerland: Springer International Publishing; p. 179–192.
   Google Scholar
• Hickman PE, Potter JM, Aroney C, Koerbin G, Southcott E, Wu AHB, Roberts MS. 2010. Cardiac troponin may be released by ischemia alone, without necrosis. Clin Chim Acta. 411:318–323.
   PubMed Web of Science ®Google Scholar
• Hillinger P, Twerenbold R, Jaeger C, Wildi K, Reichlin T, Rubini Gimenez M, Engels U, Miró O, Boeddinghaus J, Puelacher C, et al. 2015. Optimizing early rule-out strategies for acute myocardial infarction: utility of 1-hour copeptin. Clin Chem. 61:1466–1474.
   PubMed Web of Science ®Google Scholar
• Irfan A, Reichlin T, Twerenbold R, Meister M, Moehring B, Wildi K, Bassetti S, Zellweger C, Gimenez MR, Hoeller R, et al. 2013. Early diagnosis of myocardial infarction using absolute and relative changes in cardiac troponin concentrations. Am J Med. 126:781–788.e2.
   PubMed Web of Science ®Google Scholar
• Jaffe AS, Apple FS, Morrow DA, Lindahl B, Katus HA, et al. 2010. Being rational about (im)precision: a statement from the Biochemistry Subcommittee of the Joint European Society of Cardiology/American College of Cardiology Foundation/American Heart Association/World Heart Federation Task Force for the definition of myocardial infarction. Clin Chem. 56:941–943.
   PubMed Web of Science ®Google Scholar
• Jaffe AS. The 10 commandments of troponin, with special reference to high sensitivity assays. Heart. 2011;97:940–946.
   PubMed Web of Science ®Google Scholar
• Jaeger C, Wildi K, Twerenbold R, Reichlin T, Rubini Gimenez M, Neuhaus J-D, Grimm K, Boeddinghaus J, Hillinger P, Nestelberger T, et al. 2016. One-hour rule-in and rule-out of acute myocardial infarction using high-sensitivity cardiac troponin I. Am Heart J. 171:92–102.e1–5.
   PubMed Web of Science ®Google Scholar
• James S, Flodin M, Johnston N, Lindahl B, Venge P. 2006. The antibody configurations of cardiac troponin I assays may determine their clinical performance. Clin Chem. 52:832–837.
   PubMed Web of Science ®Google Scholar
• Jarolim P. 2015. High sensitivity cardiac troponin assays in the clinical laboratories. Clin Chem Lab Med. 53:635–652.
   PubMed Web of Science ®Google Scholar
• Javed U, Aftab W, Ambrose JA, Wessel RJ, Mouanoutoua M, Huang G, Barua RS, Weilert M, Sy F, Thatai D, et al. 2009. Frequency of elevated troponin I and diagnosis of acute myocardial infarction. Am J Cardiol. 104:9–13.
   PubMed Web of Science ®Google Scholar
• Jeremias A, Gibson CM. 2005. Narrative review: alternative causes for elevated cardiac troponin levels when acute coronary syndromes are excluded. Ann Intern Med. 142:786–791.
   PubMed Web of Science ®Google Scholar
• Ji M, Moon HW, Hur M, Yun YM. 2016. Determination of high-sensitivity cardiac troponin I 99th percentile upper reference limits in a healthy Korean population. Clin Biochem. 49:756–761.
   PubMed Web of Science ®Google Scholar
• Katrukha IA. 2013. Human cardiac troponin complex. Structure and functions. Biochemistry (Mosc). 78:1447–1465.
   PubMed Web of Science ®Google Scholar
• Katus HA, Remppis A, Looser S, Hallermeier K, Scheffold T, Kübler W. 1989. Enzyme linked immuno assay of cardiac troponin T for the detection of acute myocardial infarction in patients. J Mol Cell Cardiol. 21:1349–1353.
   PubMed Web of Science ®Google Scholar
• Keller T, Tzikas S, Zeller T, Czyz E, Lillpopp L, Ojeda FM, Roth A, Bickel C, Baldus S, Sinning CR, et al. 2010. Copeptin improves early diagnosis of acute myocardial infarction. J Am Coll Cardiol. 55:2096–2106.
   PubMed Web of Science ®Google Scholar
• Keller T, Zeller T, Ojeda F, Tzikas S, Lillpopp L, Sinning C, Wild P, Genth-Zotz S, Warnholtz A, Giannitsis E, et al. 2011. Serial changes in highly sensitive troponin I assay and early diagnosis of myocardial infarction. JAMA. 306:2684–2693.
   PubMed Web of Science ®Google Scholar
• Keller T, Zeller T, Peetz D, Tzikas S, Roth A, Czyz E, Bickel C, Baldus S, Warnholtz A, Fröhlich M. 2009. Sensitive troponin I assay in early diagnosis of acute myocardial infarction. N Engl J Med. 361:868–877.
   PubMed Web of Science ®Google Scholar
• Khalili H, de Lemos JA. 2014. What constitutes a relevant change in high-sensitivity troponin values over serial measurement? Clin Chem. 60:803–805.
   PubMed Web of Science ®Google Scholar
• Kimenai DM, Henry RMA, van der Kallen CJH, Dagnelie PC, Schram MT, Stehouwer CDA, van Suijlen JDE, Niens M, Bekers O, Sep SJS, et al. 2016. Direct comparison of clinical decision limits for cardiac troponin T and I. Heart. 102:610–616.
   PubMed Web of Science ®Google Scholar
• Klinkenberg LJJ, van Dijk J-W, Tan FES, van Loon LJC, van Dieijen-Visser MP, Meex SJR. 2014. Circulating cardiac troponin T exhibits a diurnal rhythm. J Am Coll Cardiol. 63:1788–1795.
   PubMed Web of Science ®Google Scholar
• Klinkenberg LJJ, Wildi K, van der Linden N, Kouw IWK, Niens M, Twerenbold R, Rubini Gimenez M, Puelacher C, Daniel Neuhaus J, Hillinger P, et al. 2016. Diurnal rhythm of cardiac troponin: consequences for the diagnosis of acute myocardial infarction. Clin Chem. 62:1602–1611.
   PubMed Web of Science ®Google Scholar
• Koerbin G, Abhayaratna WP, Potter JM, Apple FS, Jaffe AS, Ravalico TH, Hickman PE. 2013. Effect of population selection on 99th percentile values for a high sensitivity cardiac troponin I and T assays. Clin Biochem. 46:1636–1643.
   PubMed Web of Science ®Google Scholar
• Koerbin G, Potter JM, Abhayaratna WP, Telford RD, Badrick T, Apple FS, Jaffe AS, Hickman PE. 2012. Longitudinal studies of cardiac troponin I in a large cohort of healthy children. Clin Chem. 58:1665–1672.
   PubMed Web of Science ®Google Scholar
• Koerbin G, Tate J, Potter JM, Cavanaugh J, Glasgow N, Hickman PE. 2012. Characterisation of a highly sensitive troponin I assay and its application to a cardio-healthy population. Clin Chem Lab Med. 50:871–878.
   PubMed Web of Science ®Google Scholar
• Koerbin G, Tate JR, Hickman PE. 2010. Analytical characteristics of the Roche highly sensitive troponin T assay and its application to a cardio-healthy population. Ann Clin Biochem. 47:524–528.
   PubMed Web of Science ®Google Scholar
• Kozinski M, Bielis L, Wisniewska-Szmyt J, Boinska J, Stolarek W, Marciniak A, Kubica A, Grabczewska Z, Navarese EP, Andreotti F, et al. 2011. Diurnal variation in platelet inhibition by clopidogrel. Platelets. 22:579–587.
   PubMed Web of Science ®Google Scholar
• Koziński M, Bielis L, Wiśniewska-Szmyt J, Sukiennik A, Grabczewska Z, Swiatkiewicz I, Ziołkowski M, Rość D, Kubica J. 2008. Increased morning ADP-dependent platelet aggregation persists despite dual antiplatelet therapy in patients with first ST-segment elevation myocardial infarction: preliminary report. Cardiol J. 15:530–536.
   PubMed Web of Science ®Google Scholar
• Krintus M, Kozinski M, Boudry P, Lackner K, Lefèvre G, Lennartz L, Lotz J, Manysiak S, Shih J, Skadberg Ø, et al. 2015. Defining normality in a European multinational cohort: critical factors influencing the 99th percentile upper reference limit for high sensitivity cardiac troponin I. Int J Cardiol. 187:256–263.
   PubMed Web of Science ®Google Scholar
• Krintus M, Kozinski M, Boudry P, Capell NE, Köller U, Lackner K, Lefèvre G, Lennartz L, Lotz J, Herranz AM, et al. 2014. European multicenter analytical evaluation of the Abbott ARCHITECT STAT high sensitive troponin I immunoassay. Clin Chem Lab Med. 52:1657–1665.
   PubMed Web of Science ®Google Scholar
• Krintus M, Kozinski M, Fabiszak T, Kuligowska-Prusinska M, Laskowska E, Lennartz L, Nowak-Los L, Kubica J, Sypniewska G, et al. 2016. Impact of lipid markers and high-sensitivity C-reactive protein on the value of the 99th percentile upper reference limit for high-sensitivity cardiac troponin I. Clin Chim Acta. 462:193–200.
   PubMed Web of Science ®Google Scholar
• Larue C, Calzolari C, Bertinchant JP, Leclercq F, Grolleau R, Pau, B. 1993. Cardiac-specific immunoenzymometric assay of troponin I in the early phase of acute myocardial infarction. Clin Chem. 39:972–979.
   PubMed Web of Science ®Google Scholar
• Layfield C, Rose J, Alford A, Snyder SR, Apple FS, Chowdhury FM, Kontos MC, Newby LK, Storrow AB, Tanasijevic M, et al. 2015. Effectiveness of practices for improving the diagnostic accuracy of Non ST Elevation Myocardial Infarction in the Emergency Department: a Laboratory Medicine Best Practices™ systematic review. Clin Biochem. 48:204–212.
   PubMed Web of Science ®Google Scholar
• Lee G, Liu S. 2015. Monoclonal antibodies against human cardiac troponin I for immunoassays II. Monoclon Antib Immunodiagn Immunother. 34:169–173.
   PubMedGoogle Scholar
• Lee GR, Browne TCA, Guest B, Khan I, Murphy E, McGorrian C, Mahon NG, Fitzgibbon MC. 2016. Transitioning high sensitivity cardiac troponin I (hs-cTnI) into routine diagnostic use: more than just a sensitivity issue. Pract Lab Med. 4:62–75.
   PubMedGoogle Scholar
• Leuschner F, Li J, Goser S, Reinhardt L, Ottl R, Bride P, Zehelein J, Pfitzer G, Remppis A, Giannitsis E, et al. 2008. Absence of auto-antibodies against cardiac troponin I predicts improvement of left ventricular function after acute myocardial infarction. Eur Heart J. 29:1949–1955.
   PubMed Web of Science ®Google Scholar
• Lipinski MJ, Baker NC, Escárcega RO, Torguson R, Chen F, Aldous SJ, Christ M, Collinson PO, Goodacre SW, Mair J, et al. 2015. Comparison of conventional and high-sensitivity troponin in patients with chest pain: a collaborative meta-analysis. Am Heart J. 169:6–16.e6.
   PubMed Web of Science ®Google Scholar
• Lipinski MJ, Escárcega RO, D'Ascenzo F, Magalhães MA, Baker NC, Torguson R, Chen F, Epstein SE, Miró O, Llorens P, et al. 2014. A systematic review and collaborative meta-analysis to determine the incremental value of copeptin for rapid rule-out of acute myocardial infarction. Am J Cardiol. 113:1581–1591.
   PubMed Web of Science ®Google Scholar
• Lippi G, Cervellin G. 2014. Genetic polymorphisms of human cardiac troponins as an unrecognized challenge for diagnosing myocardial injury. Int J Cardiol. 171:467–470.
   PubMed Web of Science ®Google Scholar
• Lu QW, Wu XY, Morimoto S. 2013. Inherited cardiomyopathies caused by troponin mutations. J Geriatr Cardiol. 10:91–101.
   PubMedGoogle Scholar
• Macrae AR, Kavsak PA, Lustig V, Bhargava R, Vandersluis R, Palomaki GE, Yerna M-J, Jaffe AS. 2006. Assessing the requirement for the 6-hour interval between specimens in the American Heart Association Classification of Myocardial Infarction in Epidemiology and Clinical Research Studies. Clin Chem. 52:812–818.
   PubMed Web of Science ®Google Scholar
• Mbagaya W, Luvai A, Lopez B. 2015. Biological variation of cardiac troponin in stable haemodialysis patients. Ann Clin Biochem. 52:562–568.
   PubMed Web of Science ®Google Scholar
• McKie PM, Heublein DM, Scott CG, Gantzer ML, Mehta RA, Rodeheffer RJ, Redfield MM, Burnett JC, Jaffe AS. 2013. Defining high-sensitivity cardiac troponin concentrations in the community. Clin Chem. 59:1099–1107.
   PubMed Web of Science ®Google Scholar
• Melki D, Lugnegård J, Alfredsson J, Lind S, Eggers KM, Lindahl B, Jernberg T. 2015. Implications of introducing high-sensitivity cardiac troponin T Into Clinical Practice: Data From the SWEDEHEART Registry. J Am Coll Cardiol. 65:1655–1664.
   PubMed Web of Science ®Google Scholar
• Möckel M, Giannitsis E, Mueller C, Huber K, Jaffe AS, Mair J, Plebani M, Thygesen K, Lindahl B; Biomarker Study Group of the European Society of Cardiology Acute Cardiovascular Care Association. 2016. Rule-in of acute myocardial infarction: focus on troponin. Eur Heart J Acute Cardiovasc Care. Forthcoming. doi: 10.1177/2048872616653228.
   Google Scholar
• Mockel M, Searle J, Hamm C, Slagman A, Blankenberg S, Huber K, Katus H, Liebetrau C, Muller C, Muller R, et al. 2015. Early discharge using single cardiac troponin and copeptin testing in patients with suspected acute coronary syndrome (ACS): a randomized, controlled clinical process study. Eur Heart J. 36:369–376.
   PubMed Web of Science ®Google Scholar
• Mueller T, Egger M, Leitner I, Gabriel C, Haltmayer M, Dieplinger B. 2016a. Reference values of galectin-3 and cardiac troponins derived from a single cohort of healthy blood donors. Clin Chim Acta. 456:19–23.
   PubMed Web of Science ®Google Scholar
• Mueller C, Giannitsis E, Christ M, Ordóñez-Llanos J, deFilippi C, McCord J, Body R, Panteghini M, Jernberg T, Plebani M, et al. 2016b. Multicenter evaluation of a 0-hour/1-hour algorithm in the diagnosis of myocardial infarction with high-sensitivity cardiac troponin T. Ann Emerg Med. 68:76–87.
   PubMed Web of Science ®Google Scholar
• Mueller C, Giannitsis E, Möckel M, Mair J, Plebani M, Thygesen K, Jaffe AS, Lindahl B; Biomarker Study Group of the ESC Acute Cardiovascular Care Association. 2016c. Rapid rule out of acute myocardial infarction: novel biomarker-based strategies. Eur Heart J Acute Cardiovasc Care. Forthcoming. doi: 10.1177/2048872616653229.
   Google Scholar
• Mueller C. 2014. Biomarkers and acute coronary syndromes: an update. Eur Heart J. 35:552–556.
   PubMed Web of Science ®Google Scholar
• Mueller-Hennessen M, Lindahl B, Giannitsis E, Biener M, Vafaie M, deFilippi CR, Christ M, Santalo-Bel M, Panteghini M, Plebani M, et al. 2016. Diagnostic and prognostic implications using age- and gender-specific cutoffs for high-sensitivity cardiac troponin T – sub-analysis from the TRAPID-AMI study. Int J Cardiol. 209:26–33.
   PubMed Web of Science ®Google Scholar
• Neumann JT, Sörensen NA, Schwemer T, Ojeda F, Bourry R, Sciacca V, Schaefer S, Waldeyer C, Sinning C, Renné T, et al. 2016. Diagnosis of myocardial infarction using a high-sensitivity troponin I 1-hour algorithm. JAMA Cardiol. 1:397–404.
   PubMed Web of Science ®Google Scholar
• Nordenskjold AM, Ahlstrom H, Eggers KM, Frobert O, Jaffe AS, Venge P, Lindahl B. 2013. Short- and long-term individual variation in cardiac troponin in patients with stable coronary artery disease. Clin Chem. 59:401–409.
   PubMed Web of Science ®Google Scholar
• Omland T, Pfeffer MA, Solomon SD, de Lemos JA, Røsjø H, Šaltytė Benth J, Maggioni A, Domanski MJ, Rouleau JL, Sabatine MS, et al. 2013. Prognostic value of cardiac troponin I measured with a highly sensitive assay in patients with stable coronary artery disease. J Am Coll Cardiol. 61:1240–1249.
   PubMed Web of Science ®Google Scholar
• Panteghini M, Bunk DM, Christenson RH, Katrukha A, Porter RA, Schimmel H, Wang L, Tate JR, IFCC Working Group on Standardization of Troponin I. 2008. Standardization of troponin I measurements: an update. Clin Chem Lab Med. 46:1501–1506.
   PubMed Web of Science ®Google Scholar
• Panteghini M. 2004. Current concepts in standardization of cardiac marker immunoassays. Clin Chem Lab Med. 42:3–8.
   PubMed Web of Science ®Google Scholar
• Panteghini M. 2005. Standardization of cardiac troponin I measurements: the way forward? Clin Chem. 51:1594–1597.
   PubMed Web of Science ®Google Scholar
• Pecoraro V, Germagnoli L, Banfi G. 2014. Point-of-care testing: where is the evidence? A systematic survey. Clin Chem Lab Med. 52:313–324.
   PubMed Web of Science ®Google Scholar
• Raskovalova T, Twerenbold R, Collinson PO, Keller T, Bouvaist H, Folli C, Giavarina D, Lotze U, Eggers KM, Dupuy A-M, et al. 2014. Diagnostic accuracy of combined cardiac troponin and copeptin assessment for early rule-out of myocardial infarction: a systematic review and meta-analysis. Eur Heart J Acute Cardiovasc Care. 3:18–27.
   PubMedGoogle Scholar
• Reichlin T, Hochholzer W, Bassetti S, Steuer S, Stelzig C, Hartwiger S, Biedert S, Schaub N, Buerge C, Potocki M, et al. 2009. Early diagnosis of myocardial infarction with sensitive cardiac troponin assays. N Engl J Med. 361:858–867.
   PubMed Web of Science ®Google Scholar
• Reichlin T, Schindler C, Drexler B, Twerenbold R, Reiter M, Zellweger C, Moehring B, Ziller R, Hoeller R, Rubini Gimenez M, et al. 2012. One-hour rule-out and rule-in of acute myocardial infarction using high-sensitivity cardiac troponin T. Arch Intern Med. 172:1211–1218.
   PubMedGoogle Scholar
• Reichlin T, Twerenbold R, Maushart C, Reiter M, Moehring B, Schaub N, Balmelli C, Gimenez MR, Hoeller R, Sakarikos K, et al. 2013. Risk stratification in patients with unstable angina using absolute serial changes of 3 high-sensitive troponin assays. Am Heart J. 165:371–378.e3.
   PubMed Web of Science ®Google Scholar
• Reichlin T, Twerenbold R, Reiter M, Steuer S, Bassetti S, Balmelli C, Winkler K, Kurz S, Stelzig C, Freese M, et al. 2012. Introduction of high-sensitivity troponin assays: impact on myocardial infarction incidence and prognosis. Am J Med. 125:1205–1213.e1.
   PubMed Web of Science ®Google Scholar
• Reichlin T, Twerenbold R, Wildi K, Rubini Gimenez M, Bergsma N, Haaf P, Druey S, Puelacher C, Moehring B, Freese M, et al. 2015. Prospective validation of a 1-hour algorithm to rule-out and rule-in acute myocardial infarction using a high-sensitivity cardiac troponin T assay. CMAJ. 187:E243–E252.
   PubMed Web of Science ®Google Scholar
• Roffi M, Patrono C, Collet J-P, Mueller C, Valgimigli M, Andreotti F, Bax JJ, Borger MA, Brotons C, Chew DP, et al. 2016. 2015 ESC guidelines for the management of acute coronary syndromes in patients presenting without persistent ST-segment elevation: Task Force for the Management of Acute Coronary Syndromes in Patients Presenting without Persistent ST-Segment Elevation of the European Society of Cardiology (ESC). Eur Heart J. 37:267–315.
   PubMed Web of Science ®Google Scholar
• Rubini Gimenez M, Twerenbold R, Jaeger C, Schindler C, Puelacher C, Wildi K, Reichlin T, Haaf P, Merk S, Honegger U, et al. 2015. One-hour rule-in and rule-out of acute myocardial infarction using high-sensitivity cardiac troponin I. Am J Med. 128:861–870.e4.
   PubMed Web of Science ®Google Scholar
• Rubini Gimenez M, Twerenbold R, Reichlin T, Wildi K, Haaf P, Schaefer M, Zellweger C, Moehring B, Stallone F, Sou SM, et al. 2014. Direct comparison of high-sensitivity-cardiac troponin I vs. T for the early diagnosis of acute myocardial infarction. Eur Heart J. 35:2303–2311.
   PubMed Web of Science ®Google Scholar
• Ryan JB, Wallace J, Sies CW, Florkowski CM, George PM. 2015. Evaluation of Abbott Architect high-sensitivity troponin I assay for haemolysis interference. Pathology. 47:716–718.
   PubMed Web of Science ®Google Scholar
• Saenger AK, Beyrau R, Braun S, Cooray R, Dolci A, Freidank H, Giannitsis E, Gustafson S, Handy B, Katus H, et al. 2011. Multicenter analytical evaluation of a high-sensitivity troponin T assay. Clin Chim Acta. 412:748–754.
   PubMed Web of Science ®Google Scholar
• Sandoval Y, Apple FS. 2014. The global need to define normality: the 99th percentile value of cardiac troponin. Clin Chem. 60:455–462.
   PubMed Web of Science ®Google Scholar
• Sandoval Y, Apple FS, Smith SW. 2016. High-sensitivity cardiac troponin assays and unstable angina. Eur Heart J Acute Cardiovasc Care. Forthcoming. doi: 10.1177/2048872616658591.
   PubMedGoogle Scholar
• Sarko J, Pollack CV. Jr. 2002. Cardiac troponins. J Emerg Med. 23:57–65.
   PubMed Web of Science ®Google Scholar
• Saunders JT, Nambi V, de Lemos JA, Chambless LE, Virani SS, Boerwinkle E, Hoogeveen RC, Liu X, Astor BC, Mosley TH, et al. 2011. Cardiac troponin T measured by a highly sensitive assay predicts coronary heart disease, heart failure, and mortality in the Atherosclerosis Risk in Communities Study. Circulation. 123:1367–1376.
   PubMed Web of Science ®Google Scholar
• Savukoski T. 2016. Cardiac troponin specific autoantibodies: analytical tools for exploring their impact on cardiac troponin I testing. PhD thesis. University of Turku. Available from: https://www.doria.fi/bitstream/handle/10024/99057/AnnalesAII293Savukoski.pdf?sequence=2 [last accessed 10 Dec 2016].
   Google Scholar
• Scharnhorst V, Krasznai K, van’t Veer M, Michels RH. 2012. Variation of cardiac troponin I and T measured with sensitive assays in emergency department patients with noncardiac chest pain. Clin Chem. 58:1208–1214.
   PubMed Web of Science ®Google Scholar
• Schindler EI, Szymanski JJ, Hock KG, Geltman EM, Scott MG. 2016. Short- and long-term biologic variability of galectin-3 and other cardiac biomarkers in patients with stable heart failure and healthy adults. Clin Chem. 62:360–366.
   PubMed Web of Science ®Google Scholar
• Shah AS, Griffiths M, Lee KK, McAllistar AD, Hunter AL, Ferry AV, Cruikshank A, Reid A, Stoddart M, Strachan F, et al. 2015a. High sensitivity cardiac troponin and the under-diagnosis of myocardial infarction in women: prospective cohort study. BMJ. 350:g7873.
   PubMed Web of Science ®Google Scholar
• Shah ASV, Anand A, Sandoval Y, Lee KK, Smith SW, Adamson PD, Chapman AR, Langdon T, Sandeman D, Vaswani A, et al. 2015b. High-sensitivity cardiac troponin I at presentation in patients with suspected acute coronary syndrome: a cohort. Lancet. 386:2481–2488., on behalf of the High-STEACS investigators.
   PubMed Web of Science ®Google Scholar
• Simpson AJ, Potter JM, Koerbin G, Oakman C, Cullen L, Wilkes GJ, Scanlan SL, Parsonage W, Hickman PE. 2014. Use of observed within-person variation of cardiac troponin in emergency department patients for determination of biological variation and percentage and absolute reference change values. Clin Chem. 60:848–854.
   PubMed Web of Science ®Google Scholar
• Stacy SR, Suarez-Cuervo C, Berger Z, Wilson LM, Yeh H-C, Bass EB, Michos ED. 2014. Role of troponin in patients with chronic kidney disease and suspected acute coronary syndrome: a systematic review. Ann Intern Med. 161:502–512.
   PubMed Web of Science ®Google Scholar
• Steg PG, James SK, Atar D, Badano LP, Blömstrom-Lundqvist C, Borger MA, Di Mario C, Dickstein K, Ducrocq G, Fernandez-Aviles F, et al. Task Force on the management of ST-segment elevation acute myocardial infarction of the European Society of Cardiology (ESC) 2012. ESC guidelines for the management of acute myocardial infarction in patients presenting with ST-segment elevation. Eur Heart J. 33:2569–2619.
   PubMed Web of Science ®Google Scholar
• Storrow AB, Nowak RM, Diercks DB, Singer AJ, Wu AHB, Kulstad E, LoVecchio F, Fromm C, Headden G, Potis T, et al. 2015. Absolute and relative changes (delta) in troponin I for early diagnosis of myocardial infarction: results of a prospective multicenter trial. Clin Biochem. 48:260–267.
   PubMed Web of Science ®Google Scholar
• Sztefko K. 2011. Troponin measurement by immunoassay – problem of low assay sensitivity and interference from heterophilic antibodies. In: Sztefko K, editor. Immunodiagnostics and patient safety. Berlin: De Gruyter; p. 129–136.
   Google Scholar
• Tate JR, Bunk DM, Christenson RH, Barth JH, Katrukha A, Noble JE, Schimmel H, Wang L, Panteghini M, IFCC Working Group on Standardization of Cardiac Troponin I. 2015. Evaluation of standardization capability of current cardiac troponin I assays by a correlation study: results of an IFCC pilot project. Clin Chem Lab Med. 53:677–690.
   PubMed Web of Science ®Google Scholar
• Tate JR, Bunk DM, Christenson RH, Katrukha A, Noble JE, Porter RA, Schimmel H, Wang L, Panteghini M, IFCC Working Group on Standardization of Troponin I. 2010. Standardisation of cardiac troponin I measurement: past and present. Pathology. 42:402–408.
   PubMed Web of Science ®Google Scholar
• Tate JR, Panteghini M. 2013. Measurement of cardiac troponins revisited. Biochim Clin. 32:535–546.
   Google Scholar
• Ter Avest E, Visser A, Reitsma B, Breedveld R, Wolthuis A. 2016. Point-of-care troponin T is inferior to high-sensitivity troponin T for ruling out acute myocardial infarction in the emergency department. Eur J Emerg Med. 23:95–101.
   PubMed Web of Science ®Google Scholar
• Than M, Cullen L, Aldous S, Parsonage WA, Reid CM, Greenslade J, Flaws D, Hammett CJ, Beam DM, Ardagh MW, et al. 2012. 2-Hour accelerated diagnostic protocol to assess patients with chest pain symptoms using contemporary troponins as the only biomarker: the ADAPT trial. J Am Coll Cardiol. 59:2091–2098.
   PubMed Web of Science ®Google Scholar
• Than M, Cullen L, Reid CM, Lim SH, Aldous S, Ardagh MW, Peacock WF, Parsonage WA, Ho HF, Ko HF, et al. 2011. A 2-h diagnostic protocol to assess patients with chest pain symptoms in the Asia-Pacific region (ASPECT): a prospective observational validation study. Lancet. 377:1077–1084.
   PubMed Web of Science ®Google Scholar
• Than MP, Pickering JW, Aldous SJ, Cullen L, Frampton CMA, Peacock WF, Jaffe AS, Goodacre, SW, Richards AM, Ardagh MW, et al. 2016. Effectiveness of EDACS versus ADAPT accelerated diagnostic pathways for chest pain: a pragmatic randomized controlled trial embedded within practice. Ann Emerg Med. 68:93–102.
   PubMed Web of Science ®Google Scholar
• Thygesen K, Alpert JS, Jaffe AS, Simoons ML, Chaitman BR, White HD Writing Group on the Joint ESC/ACCF/AHA/WHF Task Force for the Universal Definition of Myocardial Infarction. 2012. Third universal definition of myocardial infarction. Eur Heart J. 33:2551–2567.
   PubMed Web of Science ®Google Scholar
• Thygesen K, Mair J, Giannitsis E, Mueller C, Lindahl B, Blankenberg S, Huber K, Plebani M, Biasucci LM, Tubaro M, et al. 2012. How to use high-sensitivity cardiac troponins in acute cardiac care. Eur Heart J. 33:2252–2257.
   PubMed Web of Science ®Google Scholar
• Trambas C, Pickering JW, Than M, Bain C, Nie L, Paul E, Dart A, Broughton A, Schneider HG. 2016. Impact of high-sensitivity troponin I testing with sex-specific cutoffs on the diagnosis of acute myocardial infarction. Clin Chem. 62:831–838.
   PubMed Web of Science ®Google Scholar
• Twerenbold R, Jaeger C, Rubini Gimenez M, Wildi K, Reichilin T, Nestelberger T, Boeddinghaus J, Puelacher C, Moehring B, Pretre G, et al. 2016. Impact of high-sensitivity cardiac troponin on use of coronary angiography, cardiac stress testing, and time to discharge in suspected acute myocardial infarction. Eur Heart J. 37:3324–3332.
   PubMed Web of Science ®Google Scholar
• Twerenbold R, Wildi K, Jaeger C, Gimenez MR, Reiter M, Reichlin T, Walukiewicz A, Gugala M, Krivoshei L, Marti N, et al. 2015. Optimal cutoff levels of more sensitive cardiac troponin assays for the early diagnosis of myocardial infarction in patients with renal dysfunction. Circulation. 131:2041–2050.
   PubMed Web of Science ®Google Scholar
• Ungerer JP, Tate JR, Pretorius CJ. 2016. Discordance with 3 cardiac troponin I and T assays: implications for the 99th percentile cutoff. Clin Chem. 62:1106–1114.
   PubMed Web of Science ®Google Scholar
• Using high sensitivity cardiac troponin assays in practice. 2016. Available from: http://www.ifcc.org/media/259738/201405_TF_CB_IFCC_practice%20Summary.pdf
   Google Scholar
• Vasile VC, Saenger AK, Kroning JM, Klee GG, Jaffe AS. 2011. Biologic variation of a novel cardiac troponin I assay. Clin Chem. 57:1080–1081.
   PubMed Web of Science ®Google Scholar
• Vasile VC, Saenger AK, Kroning JM, Jaffe AS. 2010. Biological and analytical variability of a novel high-sensitivity cardiac troponin T assay. Clin Chem. 56:1086–1090.
   PubMed Web of Science ®Google Scholar
• Weber M, Bazzino O, Navarro Estrada JL, de Miguel R, Salzberg S, Fuselli JJ, Liebetrau C, Woelken M, Moellmann H, Nef H, et al. 2011. Improved diagnostic and prognostic performance of a new high-sensitive troponin T assay in patients with acute coronary syndrome. Am Heart J. 162:81–88.
   PubMed Web of Science ®Google Scholar
• Wildi K, Gimenez MR, Twerenbold R, Reichlin T, Jaeger C, Heinzelmann A, Arnold C, Nelles B, Druey S, Haaf P, et al. 2015. Misdiagnosis of myocardial infarction related to limitations of the current regulatory approach to define clinical decision values for cardiac troponin. Circulation. 131:2032–2040.
   PubMed Web of Science ®Google Scholar
• Wildi K, Reichlin T, Twerenbold R, Mäder F, Zellweger C, Moehring B, Stallone F, Minners J, Rubini Gimenez M, Hoeller R, et al. 2013. Serial changes in high-sensitivity cardiac troponin I in the early diagnosis of acute myocardial infarction. Int J Cardiol. 168:4103–4110.
   PubMed Web of Science ®Google Scholar
• Wu AH, Akhigbe P, Wians F. 2012. Long-term biological variation in cardiac troponin I. Clin Biochem. 45:714–716.
   PubMed Web of Science ®Google Scholar
• Wu AH, Christenson RH. 2013. Analytical and assay issues for use of cardiac troponin testing for risk stratification in primary care. Clin Biochem. 46:969–978.
   PubMed Web of Science ®Google Scholar
• Wu AH, Feng YJ, Moore R, Apple FS, McPherson PH, Buechler KF, Bodor G. 1998. Characterization of cardiac troponin subunit release into serum after acute myocardial infarction and comparison of assays for troponin T and I. American Association for Clinical Chemistry Subcommittee on cTnI Standardization. Clin Chem. 44:1198–1208.
   PubMed Web of Science ®Google Scholar
• Wu AH, Lu QA, Todd J, Moecks J, Wians F. 2009. Short- and long-term biological variation in cardiac troponin I measured with a high-sensitivity assay: implications for clinical practice. Clin Chem. 55:52–58.
   PubMed Web of Science ®Google Scholar
• Zeller T, Ojeda F, Brunner FJ, Peitsmeyer P, Münzel T, Binder H, Pfeiffer N, Michal M, Wild PS, Blankenberg S, et al. 2015. High-sensitivity cardiac troponin I in the general population – defining reference populations for the determination of the 99th percentile in the Gutenberg Health Study. Clin Chem Lab Med. 53:699–706.
   PubMed Web of Science ®Google Scholar