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Review Article

Sample management for clinical biochemistry assays: Are serum and plasma interchangeable specimens?

ORCID Icon, ORCID Icon, ORCID Icon & ORCID Icon
Pages 480-500 | Received 22 Apr 2018, Accepted 09 Jul 2018, Published online: 12 Oct 2018

References

  • Lima-Oliveira G, Volanski W, Lippi G, et al. Pre-analytical phase management: a review of the procedures from patient preparation to laboratory analysis. Scand J Clin Lab Invest. 2017;77:153–163.
  • Lima-Oliveira G, Lippi G, Salvagno GL, et al. Laboratory diagnostics and quality of blood collection. J Med Biochem. 2015;34:288–294.
  • Lima-Oliveira G, Lippi G, Salvagno GL, et al. The effective reduction of tourniquet application time after minor modification of the CLSI H03-A6 blood collection procedure. Biochemia Medica. 2013;23:308–315.
  • Silva D, Ponte CG, Hacker MA, et al. A whole blood assay as a simple, broad assessment of cytokines and chemokines to evaluate human immune responses to Mycobacterium tuberculosis antigens. Acta Trop. 2013;127:75–81.
  • Silberer J, Ihorst G, Kopp MV. Cytokine levels in supernatants of whole blood and mononuclear cell cultures in adults and neonates reveal significant differences with respect to interleukin-13 and interferon-gamma. Pediatr Allergy Immunol. 2008;19:140–147.
  • Kost GJ, McQueen MJ. New whole blood analyzers and their impact on cardiac and critical care. Crit Rev Clin Lab Sci. 1993;30:153–202.
  • Tang R, Yang H, Choi JR, et al. Capillary blood for point-of-care testing. Crit Rev Clin Lab Sci. 2017;54:294–308.
  • World Health Organization W. Use of anticoagulants in diagnostic laboratory investigations. Geneva: WHO and WHO/DIL/LAB/99.1 Rev.2; 2002.
  • International Organization for Standardization. Medical laboratories – requirements for quality and competence iso document 15189. Geneva, Switzerland: International Organization for Standardization; 2012.
  • Lundblad R. Considerations for the use of blood plasma and serum for proteomic analysis. Int J Genomics Proteomics. 2003;1:1–8.
  • Lima-Oliveira G, Salvagno GL, Lippi G, et al. Quality management of preanalytical phase: impact of lithium heparin vacuum tubes changes on clinical chemistry tests. Accred Qual Assur. 2013;18:429–434.
  • Lima-Oliveira G, Lippi G, Salvagno GL, et al. Preanalytical management: serum vacuum tubes validation for routine clinical chemistry. Biochemia Medica. 2012;22:180–186.
  • Clinical Laboratory Standards Institute. Procedures for the handling and processing of blood specimens for common laboratory tests. CLSI GP44-A4 (formally CLSI H18-A4). Wayne, (PA): Clinical Laboratory Standards Institute; 2010.
  • van Geest-Daalderop JH, Mulder AB, Boonman-de Winter LJ, et al. Preanalytical variables and off-site blood collection: influences on the results of the prothrombin time/international normalized ratio test and implications for monitoring of oral anticoagulant therapy. Clin Chem. 2005;51:561–568.
  • Kataoka K, Yamada S, Toki N. Effects of cryoglobulin on fibrin clot formation and fibrinolysis. Acta Haematol. 1984;71:90–96.
  • Etienne J, Sarmini H, Borgard JP, et al. Biochemical problems caused by some cryoglobulins. Biomedicine. 1978;29:117–120.
  • Ali D, Sacchetto E, Dumontet E, et al. Hemolysis influence on twenty-two biochemical parameters measurement. Annales De Biologie Clinique. 2014;72:297–311.
  • Monneret D, Mestari F, Atlan G, et al. Hemolysis indexes for biochemical tests and immunoassays on Roche analyzers: determination of allowable interference limits according to different calculation methods. Scand J Clin Lab Invest. 2015;75:162–169.
  • Fernandez P, Llopis MA, Perich C, et al. Harmonization in hemolysis detection and prevention. A working group of the Catalonian Health Institute (ICS) experience. Clin Chem Lab Med. 2014;52:1557–1568.
  • Lippi G, Salvagno GL, Montagnana M, et al. Influence of hemolysis on routine clinical chemistry testing. Clin Chem Lab Med. 2006;44:311–316.
  • Friedel R, Mattenheimer H. Release of metabolic enzymes from platelets during blood clotting of man, dog, rabbit and rat. Clin Chim Acta. 1970;30:37–46.
  • Tamechika Y, Iwatani Y, Tohyama K, et al. Insufficient filling of vacuum tubes as a cause of microhemolysis and elevated serum lactate dehydrogenase levels. Use of a data-mining technique in evaluation of questionable laboratory test results. Clin Chem Lab Med. 2006;44:657–661.
  • Cui M, Jing R, Wang H. Changes of serum lactate dehydrogenase and potassium levels produced by a pneumatic tube system. Lab Med. 2009;40:728–731.
  • Lippi G, Musa R, Battistelli L, et al. Relationship between sampling volume of primary serum tubes and spurious hemolysis. Clin Lab. 2012;58:1187–1191.
  • Kapoula GV, Kontou PI, Bagos PG. The impact of pneumatic tube system on routine laboratory parameters: a systematic review and meta-analysis. Clin Chem Lab Med. 2017;55:1834–1844.
  • Lippi G, Avanzini P, Cosmai M, et al. Incomplete filling of lithium heparin tubes affects the activity of creatine kinase and gamma-glutamyltransferase. Br J Biomed Sci. 2012;69:67–70.
  • Bockel-Frohnhofer N, Hubner U, Hummel B, et al. Pneumatic tube-transported blood samples in lithium heparinate gel separator tubes may be more susceptible to haemolysis than blood samples in serum tubes. Scand J Clin Lab Invest. 2014;74:599–602.
  • Pasqualetti S, Szoke D, Panteghini M. Heparinate but not serum tubes are susceptible to hemolysis by pneumatic tube transportation. Clin Chem Lab Med. 2016;54:785–789.
  • Leino A, Koivula MK. Stability of chemical and immunochemical analytes in uncentrifuged plasma samples. Ann Clin Biochem. 2009;46:159–161.
  • Tanner M, Kent N, Smith B, et al. Stability of common biochemical analytes in serum gel tubes subjected to various storage temperatures and times pre-centrifugation. Ann Clin Biochem. 2008;45:375–379.
  • Lima-Oliveira G, Lippi G, Salvagno GL, et al. Does laboratory automation for the preanalytical phase improve data quality? J Lab Autom. 2013;18:375–381.
  • Lippi G, Lima-Oliveira G, Nazer SC, et al. Suitability of a transport box for blood sample shipment over a long period. Clin Biochem. 2011;44:1028–1029.
  • Stahl M, Brandslund I. Controlled storage conditions prolong stability of biochemical components in whole blood. Clin Chem Lab Med. 2005;43:210–215.
  • Rehak NN, Chiang BT. Storage of whole blood: effect of temperature on the measured concentration of analytes in serum. Clin Chem. 1988;34:2111–2114.
  • van Balveren JA, Huijskens MJ, Gemen EF, et al. Effects of time and temperature on 48 routine chemistry, haematology and coagulation analytes in whole blood samples. Ann Clin Biochem. 2017;54:448–462.
  • Sciacovelli L, Lippi G, Sumarac Z, et al. Quality Indicators in Laboratory Medicine: the status of the progress of IFCC Working Group "Laboratory Errors and Patient Safety" project. Clin Chem Lab Med. 2017;55:348–357.
  • Monneret D, Corlouer C, Bigot J, et al. Comparison of a 10- vs. 15-min centrifugation time for chemical and immunochemical assays and impact on turnaround time in a hospital laboratory. Clin Chem Lab Med. 2016;54:e117–e121.
  • Holland L, DomBourian M. Evaluation of an abbreviated centrifugation protocol for chemistry testing. Lab Med. 2012;43:78–81.
  • Minder EI, Schibli A, Mahrer D, et al. Effects of different centrifugation conditions on clinical chemistry and Immunology test results. BMC Clin Pathol. 2011;11:6.
  • Lippi G, Salvagno GL, Montagnana M, et al. Preparation of a quality sample: effect of centrifugation time on stat clinical chemistry testing. Lab Med. 2007;38:172–176.
  • Koenders MM, van Hurne ME, Glasmacher-Van Zijl M, et al. The analytic impact of a reduced centrifugation step on chemistry and immunochemistry assays: an evaluation of the Modular Pre-Analytics. Ann Clin Biochem. 2012;49:468–474.
  • Moller MF, Sondergaard TR, Kristensen HT, et al. Evaluation of a reduced centrifugation time and higher centrifugal force on various general chemistry and immunochemistry analytes in plasma and serum. Ann Clin Biochem. 2017;54:593–600.
  • Lima-Oliveira G, Lippi G, Salvagno GL, et al. Abnormal gel flotation caused by contrast media during adrenal vein sampling. Biochem Med. 2016;26:444–450.
  • Bowen RA, Hortin GL, Csako G, et al. Impact of blood collection devices on clinical chemistry assays. Clin Biochem. 2010;43:4–25.
  • Sniegoski LT, Moody JR. Determination of serum and blood densities. Anal Chem. 1979;51:1577–1578.
  • Hinghofer-Szalkay H, Greenleaf JE. Continuous monitoring of blood volume changes in humans. J Appl Physiol. 1987;63:1003–1007.
  • Hira K, Ohtani Y, Rahman M, et al. Pseudohyperkalaemia caused by recentrifugation of blood samples after storage in gel separator tubes. Ann Clin Biochem. 2001;38:386–390.
  • Hue DP, Culank LS, Toase PD, et al. Observed changes in serum potassium concentration following repeat centrifugation of Sarstedt Serum Gel Safety Monovettes after storage. Ann Clin Biochem. 1991;28:309–310.
  • Bio-RAD [Internet]. The Bio-plex success guide for multiplex immunoassays [cited 2018 Apr 10]; Available from: http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6458.pdf
  • BD. BD Vacutainer® Barricor™ Plasma Blood Collection Tube: BD; 2017 [cited 2018 Apr 16]. Available from: http://barricor.bd.com/eu/harness-the-power-of-centrifugation.xml.
  • Arslan FD, Karakoyun I, Basok BI, et al. The local clinical validation of a new lithium heparin tube with a barrier: BD Vacutainer® Barricor LH Plasma tube. Biochem Med (Zagreb). 2017;27:030706.
  • Cadamuro J, Mrazek C, Leichtle AB, et al. Influence of centrifugation conditions on the results of 77 routine clinical chemistry analytes using standard vacuum blood collection tubes and the new BD-Barricor tubes. Biochem Med (Zagreb). 2018;28:010704.
  • Lippi G, Salvagno GL, Danese E, et al. Inversion of lithium heparin gel tubes after centrifugation is a significant source of bias in clinical chemistry testing. Clin Chim Acta. 2014;436:183–187.
  • Brandhorst G, Engelmayer J, Gotze S, et al. Pre-analytical effects of different lithium heparin plasma separation tubes in the routine clinical chemistry laboratory. Clin Chem Lab Med. 2011;49:1473–1477.
  • Da Rin G, Lippi G. The quality of diagnostic testing may be impaired during shipment of lithium-heparin gel tubes. Clin Chem Lab Med. 2014;52:1633–1637.
  • Balbas LA, Amaro MS, Rioja RG, et al. Stability of plasma electrolytes in Barricor and PST II tubes under different storage conditions. Biochem Med. 2017;27:225–230.
  • Zungun C, Yilmaz FM, Boru EG, et al. Comparison of Improvacuter tubes with BD Vacutainer tubes for various hormones in the aspects of stability and influence of gel separators. Clin Chem Lab Med. 2015;53:231–238.
  • Bowen RA, Remaley AT. Interferences from blood collection tube components on clinical chemistry assays. Biochem Med. 2014;24:31–44.
  • Bowen RA, Adcock DM. Blood collection tubes as medical devices: the potential to affect assays and proposed verification and validation processes for the clinical laboratory. Clin Biochem. 2016;49:1321–1330.
  • Lima-Oliveira G, Lippi G, Salvagno GL, et al. Sodium citrate vacuum tubes validation: preventing preanalytical variability in routine coagulation testing. Blood Coagul Fibrinolysis. 2013;24:252–255.
  • Lima-Oliveira G, Lippi G, Salvagno GL, et al. Brand of dipotassium EDTA vacuum tube as a new source of pre-analytical variability in routine haematology testing. Br J Biomed Sci. 2013;70:6–9.
  • Lima-Oliveira G, Lippi G, Salvagno GL, et al. K(3)EDTA vacuum tubes validation for routine hematological testing. ISRN Hematol. 2012;2012:875357.
  • Lippi G, Cornes MP, Grankvist K, et al. EFLM WG-Preanalytical phase opinion paper: local validation of blood collection tubes in clinical laboratories. Clin Chem Lab Med. 2016;54:755–760.
  • Giavarina D, Banfi G, Daves M, et al. Blood collection systems in clinical laboratories: local adaptation of the EFLM guidelines. Biochim Clin. 2016;40:347–352.
  • Clinical Laboratory Standards Institute. Validation and verification of tubes for venous and capillary blood specimen collection. CLSI GP34-A. Wayne (PA): Clinical Laboratory Standards Institute; 2010.
  • Lum G, Gambino SR. A comparison of serum versus heparinized plasma for routine chemistry tests. Am J Clin Pathol. 1974;61:108–113.
  • Ladenson JH, Tsai LM, Michael JM, et al. Serum versus heparinized plasma for eighteen common chemistry tests: is serum the appropriate specimen? Am J Clin Pathol. 1974;62:545–552.
  • Doumas BT, Hause LL, Simuncak DM, et al. Differences between values for plasma and serum in tests performed in the Ektachem 700 XR Analyzer, and evaluation of “plasma separator tubes (PST)”. Clin Chem. 1989;35:151–153.
  • Miles RR, Roberts RF, Putnam AR, et al. Comparison of serum and heparinized plasma samples for measurement of chemistry analytes. Clin Chem. 2004;50:1704–1706.
  • Ricos C, Alvarez V, Cava F, et al. Current databases on biological variation: pros, cons and progress. Scand J Clin Lab Invest. 1999;59:491–500.
  • Sandberg S, Fraser CG, Horvath AR, et al. Defining analytical performance specifications: Consensus Statement from the 1st Strategic Conference of the European Federation of Clinical Chemistry and Laboratory Medicine. Clin Chem Lab Med. 2015;53:833–835.
  • Ceriotti F, Fernandez-Calle P, Klee GG, et al. Criteria for assigning laboratory measurands to models for analytical performance specifications defined in the 1st EFLM Strategic Conference. Clin Chem Lab Med. 2017;55:189–194.
  • Zhao X, Qureshi F, Eastman PS, et al. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. J Immunol Methods. 2012;378:72–80.
  • van de Kerkhof D, Peters B, Scharnhorst V. Troponin I concentrations in heparinized plasma and serum differ when measured with the Advia Centaur TnI-Ultra assay. Scand J Clin Lab Invest. 2008;68:513–515.
  • Pagani F, Stefini F, Chapelle JP, et al. Multicenter evaluation of analytical performance of the Liaison troponin I assay. Clin Biochem. 2004;37:750–757.
  • Dominici R, Infusino I, Valente C, et al. Plasma or serum samples: measurements of cardiac troponin T and of other analytes compared. Clin Chem Lab Med. 2004;42:945–951.
  • Gerhardt W, Nordin G, Herbert AK, et al. Troponin T and I assays show decreased concentrations in heparin plasma compared with serum: lower recoveries in early than in late phases of myocardial injury. Clin Chem. 2000;46:817–821.
  • Stiegler H, Fischer Y, Vazquez-Jimenez JF, et al. Lower cardiac troponin T and I results in heparin-plasma than in serum. Clin Chem. 2000;46:1338–1344.
  • Katrukha A, Bereznikova A, Filatov V, et al. Biochemical factors influencing measurement of cardiac troponin I in serum. Clin Chem Lab Med. 1999;37:1091–1095.
  • Capolaghi B, Charbonnier B, Dumontet M, et al. Prescription, assay and interpretation of cardiac troponins tests: guidelines from SFBC-CNBC troponin working group. Ann Biol Clin (Paris). 2005;63:245–261.
  • Kronenberg F, Trenkwalder E, Kronenberg MF, et al. Influence of hematocrit on the measurement of lipoproteins demonstrated by the example of lipoprotein(a). Kidney Int. 1998;54:1385–1389.
  • Freer DE, Statland BE, Johnson M, et al. Reference values for selected enzyme activities and protein concentrations in serum and plasma derived from cord-blood specimens. Clin Chem. 1979;25:565–569.
  • Bakker AJ, Bakker A, Renting-Wiering H. Cellular content in plasma of Becton-Dickinson lithium-heparin tubes: cause of unreliable results in the IFCC-recommended lactate dehydrogenase method. Ann Clin Biochem. 2006;43:510–512.
  • Rothwell DJ, Jendrzejczak B, Becker M, et al. Lactate dehydrogenase activities in serum and plasma. Clin Chem. 1976;22:1024–1026.
  • Hollaar L, Van der Laarse A. Interference of the measurement of lactate dehydrogenase (LDH) activity in human serum and plasma by LDH from blood cells. Clin Chim Acta. 1979;99:135–142.
  • Ono T, Kitaguchi K, Takehara M, et al. Serum-constituents analyses: effect of duration and temperature of storage of clotted blood. Clin Chem. 1981;27:35–38.
  • Coppinger JA, Cagney G, Toomey S, et al. Characterization of the proteins released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions. Blood. 2004;103:2096–2104.
  • Wolf P. The nature and significance of platelet products in human plasma. Br J Haematol. 1967;13:269–288.
  • Lippi G, Salvagno GL, Montagnana M, et al. Quality standards for sample collection in coagulation testing. Semin Thromb Hemost. 2012;38:565–575.
  • Nordestgaard BG, Langsted A, Mora S, et al. Fasting is not routinely required for determination of a lipid profile: clinical and laboratory implications including flagging at desirable concentration cutpoints-a joint consensus statement from the European Atherosclerosis Society and European Federation of Clinical Chemistry and Laboratory Medicine. Clin Chem. 2016;62:930–946.
  • Ramasamy I. Update on the laboratory investigation of dyslipidemias. Clin Chim Acta. 2018;479:103–125.
  • O'Keane MP, Cunningham SK. Evaluation of three different specimen types (serum, plasma lithium heparin and serum gel separator) for analysis of certain analytes: clinical significance of differences in results and efficiency in use. Clin Chem Lab Med. 2006;44:662–668.
  • Sassolas A, Cheillan D, Drai J, et al. Should blood samples be drawn on heparin-anticoagulant for lipid analysis? Ann Biol Clin (Paris). 2004;62:583–586.
  • Ishikawa M, Tajima Y, Murayama M, et al. Plasma and serum from nonfasting men and women differ in their lipidomic profiles. Biol Pharm Bull. 2013;36:682–685.
  • Denery JR, Nunes AA, Dickerson TJ. Characterization of differences between blood sample matrices in untargeted metabolomics. Anal Chem. 2011;83:1040–1047.
  • Al-Kharusi A, Al-Lawati N, Al-Kindi M, et al. Are tubes containing sodium fluoride still needed for the measurement of blood glucose in hospital laboratory practice? Oman Med J. 2014;29:404–407.
  • Sacks DB, Arnold M, Bakris GL, et al. Guidelines and recommendations for laboratory analysis in the diagnosis and management of diabetes mellitus. Diabetes Care. 2011;34:e61–e99.
  • American Diabetes A. Standards of medical care in diabetes–2010. Diabetes Care. 2010;33:S11–S61.
  • World Health Organization W. Definition and diagnosis of diabetes mellitus and intermediate hyperglycemia: report of a WHO/IDF consultation. Geneva: World Health Organization; 2006.
  • Chan AY, Swaminathan R, Cockram CS. Effectiveness of sodium fluoride as a preservative of glucose in blood. Clin Chem. 1989;35:315–317.
  • Gambino R, Piscitelli J, Ackattupathil TA, et al. Acidification of blood is superior to sodium fluoride alone as an inhibitor of glycolysis. Clin Chem. 2009;55:1019–1021.
  • Fobker M. Stability of glucose in plasma with different anticoagulants. Clin Chem Lab Med. 2014;52:1057–1060.
  • Peake MJ, Bruns DE, Sacks DB, et al. It's time for a better blood collection tube to improve the reliability of glucose results. Diabetes Care. 2013;36:e2.
  • Carey R, Lunt H, Heenan HF, et al. Collection tubes containing citrate stabiliser over-estimate plasma glucose, when compared to other samples undergoing immediate plasma separation. Clin Biochem. 2016;49:1406–1411.
  • von Eckardstein A, Roth HJ, Jones G, et al. cobas 8000 Modular analyzer series evaluated under routine-like conditions at 14 sites in Australia, Europe, and the United States. J Lab Autom. 2013;18:306–327.
  • Goodwin ML, Harris JE, Hernandez A, et al. Blood lactate measurements and analysis during exercise: a guide for clinicians. J Diabetes Sci Technol. 2007;1:558–569.
  • Boyanton BL, Jr, Blick KE. Stability studies of twenty-four analytes in human plasma and serum. Clin Chem. 2002;48:2242–2247.
  • Mikesh LM, Bruns DE. Stabilization of glucose in blood specimens: mechanism of delay in fluoride inhibition of glycolysis. Clin Chem. 2008;54:930–932.
  • Goldstein BN, Wesler J, Nowacki AS, et al. Investigations of blood ammonia analysis: test matrices, storage, and stability. Clin Biochem. 2017;50:537–539.
  • Dukic L, Simundic AM. Short-term and long-term storage stability of heparin plasma ammonia. J Clin Pathol. 2015;68:288–291.
  • Ong JP, Aggarwal A, Krieger D, et al. Correlation between ammonia levels and the severity of hepatic encephalopathy. Am J Med. 2003;114:188–193.
  • Wolberg AS, Campbell RA. Thrombin generation, fibrin clot formation and hemostasis. Transfus Apher Sci. 2008;38:15–23.
  • Ferry JD, Morrison PR. Preparation and properties of serum and plasma proteins; the conversion of human fibrinogen to fibrin under various conditions. J Am Chem Soc. 1947;69:388–400.
  • Carr ME, Jr, Gabriel DA, McDonagh J. Influence of Ca2+ on the structure of reptilase-derived and thrombin-derived fibrin gels. Biochem J. 1986;239:513–516.
  • Karpukhin LE, Feofanova MA, Nikolaeva LS, et al. Complexation of magnesium and calcium ions with heparin. Russ J Inorg Chem. 2006;51:908–914.
  • Stevic I, Parmar N, Paredes N, et al. Binding of heparin to metals. Cell Biochem Biophys. 2011;59:171–178.
  • Chevalier F, Angulo J, Lucas R, et al. The heparin-Ca2+ interaction: structure of the Ca2+ binding site. Eur J Org Chem. 2002;2002:2367–2376.
  • Boink AB, Buckley BM, Christiansen TF, et al. International Federation of Clinical Chemistry (IFCC) scientific division IFCC recommendation. Recommendation on sampling, transport and storage for the determination of the concentration of ionized calcium in whole blood, plasma and serum. Ann Biol Clin (Paris). 1991;49:434–438.
  • Landt M, Hortin GL, Smith CH, et al. Interference in ionized calcium measurements by heparin salts. Clin Chem. 1994;40:565–570.
  • Howard MR, Ashwell S, Bond LR, et al. Artefactual serum hyperkalaemia and hypercalcaemia in essential thrombocythaemia. J Clin Pathol. 2000;53:105–109.
  • Babic N, Zibrat S, Gordon IO, et al. Effect of blood collection tubes on the incidence of artifactual hyperkalemia on patient samples from an outreach clinic. Clin Chim Acta. 2012;413:1454–1458.
  • Nijsten MW, de Smet BJ, Dofferhoff AS. Pseudohyperkalemia and platelet counts. N Engl J Med. 1991;325:1107.
  • Lee HK, Brough TJ, Curtis MB, et al. Pseudohyperkalemia–is serum or whole blood a better specimen type than plasma? Clin Chim Acta. 2008;396:95–96.
  • Sevastos N, Theodossiades G, Archimandritis AJ. Pseudohyperkalemia in serum: a new insight into an old phenomenon. Clin Med Res. 2008;6:30–32.
  • Thurlow V, Ozevlat H, Jones SA, et al. Establishing a practical blood platelet threshold to avoid reporting spurious potassium results due to thrombocytosis. Ann Clin Biochem. 2005;42:196–199.
  • Ranjitkar P, Greene DN, Baird GS, et al. Establishing evidence-based thresholds and laboratory practices to reduce inappropriate treatment of pseudohyperkalemia. Clin Biochem. 2017;50:663–669.
  • Ahmed R, Isaac AM. Postsplenectomy thrombocytosis and pseudohyperkalemia in trauma: a case report and review of literature. J Trauma. 2009;67:E17–E19.
  • Garwicz D, Karlman M. Early recognition of reverse pseudohyperkalemia in heparin plasma samples during leukemic hyperleukocytosis can prevent iatrogenic hypokalemia. Clin Biochem. 2012;45:1700–1702.
  • Meng QH, Krahn J. Reverse pseudohyperkalemia in heparin plasma samples from a patient with chronic lymphocytic leukemia. Clin Biochem. 2011;44:728–730.
  • Lippi G, Salvagno GL, Guidi GC. Adjustment of serum potassium for age and platelet count. A simple step forward towards personalized medicine. Clin Chem Lab Med. 2015;53:e325–e327.
  • Lippi G, Salvagno GL, Guidi GC. Mean corpuscular volume and red blood cell distribution width are independent predictors of serum potassium concentration in healthy individuals. Clin Chim Acta. 2015;446:117–118.
  • Macho L. Changes of organic phosphate esters during glycolysis in blood followed by a paper chromatography method. Nature. 1957;180:1351–1352.
  • Henriksen LO, Faber NR, Moller MF, et al. Stability of 35 biochemical and immunological routine tests after 10 hours storage and transport of human whole blood at 21 degrees C. Scand J Clin Lab Invest. 2014;74:603–610.
  • Oddoze C, Lombard E, Portugal H. Stability study of 81 analytes in human whole blood, in serum and in plasma. Clin Biochem. 2012;45:464–469.
  • Anker P, Mulcahy H, Stroun M. Circulating nucleic acids in plasma and serum as a noninvasive investigation for cancer: time for large-scale clinical studies?. Int J Cancer. 2003;103:149–152.
  • Jung M, Klotzek S, Lewandowski M, et al. Changes in concentration of DNA in serum and plasma during storage of blood samples. Clin Chem. 2003;49:1028–1029.
  • Thijssen MA, Swinkels DW, Ruers TJ, et al. Difference between free circulating plasma and serum DNA in patients with colorectal liver metastases. Anticancer Res. 2002;22:421–425.
  • Almeida MI, Reis RM, Calin GA. MicroRNA history: discovery, recent applications, and next frontiers. Mutat Res. 2011;717:1–8.
  • Danese E, Minicozzi AM, Benati M, et al. Reference miRNAs for colorectal cancer: analysis and verification of current data. Sci Rep. 2017;7:8413.
  • Wang K, Yuan Y, Cho JH, et al. Comparing the MicroRNA spectrum between serum and plasma. PLoS One. 2012;7:e41561.
  • Osman A, Falker K. Characterization of human platelet microRNA by quantitative PCR coupled with an annotation network for predicted target genes. Platelets. 2011;22:433–441.
  • Schnabel RB, Baumert J, Barbalic M, et al. Duffy antigen receptor for chemokines (Darc) polymorphism regulates circulating concentrations of monocyte chemoattractant protein-1 and other inflammatory mediators. Blood. 2010;115:5289–5299.
  • Yatomi Y, Igarashi Y, Yang L, et al. Sphingosine 1-phosphate, a bioactive sphingolipid abundantly stored in platelets, is a normal constituent of human plasma and serum. J Biochem. 1997;121:969–973.
  • Teerlink T, Nijveldt RJ, de Jong S, et al. Determination of arginine, asymmetric dimethylarginine, and symmetric dimethylarginine in human plasma and other biological samples by high-performance liquid chromatography. Anal Biochem. 2002;303:131–137.
  • Yu Z, Kastenmuller G, He Y, et al. Differences between human plasma and serum metabolite profiles. PLoS One. 2011;6:e21230.
  • Liu L, Aa J, Wang G, et al. Differences in metabolite profile between blood plasma and serum. Anal Biochem. 2010;406:105–112.
  • Kuzuya M, Iguchi A. Role of matrix metalloproteinases in vascular remodeling. J Atheroscler Thromb. 2003;10:275–282.
  • Mannello F. Serum or plasma samples? The "Cinderella" role of blood collection procedures: preanalytical methodological issues influence the release and activity of circulating matrix metalloproteinases and their tissue inhibitors, hampering diagnostic trueness and leading to misinterpretation. Arterioscler Thromb Vasc Biol. 2008;28:611–614.
  • Breier M, Wahl S, Prehn C, et al. Targeted metabolomics identifies reliable and stable metabolites in human serum and plasma samples. PLoS One. 2014;9:e89728.
  • Suarez-Diez M, Adam J, Adamski J, et al. Plasma and serum metabolite association networks: comparability within and between studies using NMR and MS profiling. J Proteome Res. 2017;16:2547–2559.
  • Gar C, Rottenkolber M, Prehn C, et al. Serum and plasma amino acids as markers of prediabetes, insulin resistance, and incident diabetes. Crit Rev Clin Lab Sci. 2018;55:21–32.
  • Leek JT, Scharpf RB, Bravo HC, et al. Tackling the widespread and critical impact of batch effects in high-throughput data. Nat Rev Genet. 2010;11:733–739.
  • Lippi G, Lima-Oliveira G, Brocco G, et al. Estimating the intra- and inter-individual imprecision of manual pipetting. Clin Chem Lab Med. 2017;55:962–966.
  • Flanagan RJ, Brow NW, Whelpton R. Therapeutic Drug Monitoring (TDM). CPD Clin Biochem. 2008;9:3–21.
  • Uges DR. Plasma or serum in therapeutic drug monitoring and clinical toxicology. Pharm Weekbl Sci. 1988;10:185–188.
  • O'Connell ME, Heim KL, Halstenson CE, et al. Analytical accuracy of determinations of aminoglycoside concentrations by enzyme multiplied immunoassay, fluorescence polarization immunoassay, and radioimmunoassay in the presence of heparin. J Clin Microbiol. 1984;20:1080–1082.
  • Dubrowny NE, Harrop AJ. Inventors collection device. United States Patent US 6,686,204 B2; 2004 Feb 3.
  • Sylte MS, Wentzel-Larsen T, Bolann BJ. Estimation of the minimal preanalytical uncertainty for 15 clinical chemistry serum analytes. Clin Chem. 2010;56:1329–1335.

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