Summary of available data for cholesterol in foods and methods for its determinationFootnote∗

Mention of a trademark or propietary product does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable.

References

• Abell , L. L. , Levy , B. B. , Brodie , B. B. and Kendall , F. E. 1952 . A simplified method for estimation of total cholesterol in serum and demonstration of its specificity . J. Biol. Chem. , 195 : 357
   PubMed Web of Science ®Google Scholar
• Adamson , L. F. , Leeper , G. K. and Ross , E. 1961 . Influence of dietary fats and cholesterol on tissue lipids in chicken . J. Nutr. , 73 : 247
   Web of Science ®Google Scholar
• Addison , R. F. , Ackman , R. G. and Hingley , J. 1968 . Distribution of fatty acids in cod flesh lipids . J. Fish. Res. Board Can. , 25 : 2083
   Web of Science ®Google Scholar
• Albers , W. and Louiry , O. H. 1955 . Fluorometric determination of 0.1 to 10 micrograms of cholesterol . Anal. Chem. , 27 : 1829
   Google Scholar
• Allain , C. C. , Poon , L. S. , Chan , C. , Richmond , W. and Fu , C. P. 1974 . Enzymatic determination of total serum cholesterol . Clin. Chem. , 20 : 470
   PubMed Web of Science ®Google Scholar
• Allen , E. , Cassens , R. G. and Bray , R. W. 1967 . Comparative lipid composition of three porcine muscles . J. Anim. Sci. , 26 : 36
   Google Scholar
• Ansbacher , S. and Supplee , G. C. 1934 . The cholesterol content and the antirachitic activation of milk constituents . J. Biol. Chem. , 105 : 391
   Google Scholar
• Archard , Ch. , Levy , J. and Georgiakakis , N. 1934 . Le cholesterol des Aliments. . Arch. Mal. Appar. Dig. Mai. Nutr. , 24 : 785
   Google Scholar
• Azim , M. A. , Rana , A. and Kiani , A. 1968 . The change in cholesterol content of eggs while cooking . Pak. J. Med. Res. , 7 : 74
   Google Scholar
• Baker , D. R. , Henry , R. A. , Williams , R. C. , Hudson , D. R. and Parris , N. A. 1973 . Preparative columns in high‐speed liquid chromatography . J. Chrom. , 83 : 233
   Google Scholar
• Bartov , I. , Bornstein , S. and Budowski , P. 1971 . Variability of cholesterol concentration in plasma and egg yolk of hens and evaluation of the effect of some dietary oils. . Poult. Sci. , 50 : 1357
   PubMed Web of Science ®Google Scholar
• Bennett , R. D. and Heftmann , E. 1962 . Thin‐layer chromatography of sterols . J. Chromatogr. , 9 : 359
   Google Scholar
• Bergmeyer , H. , Gruber , W. , Bernt , E. , Hagen , A. , Roeschalau , P. , Lung , G. and Beaucamp , K. Determination of Cholesterol . German Patent Appl. 2224132–52 . 1972 .
   Google Scholar
• Bligh , E. G. and Scott , M. A. 1966 . Lipids of cod muscle and the effect of frozen storage . J. Fish. Res. Board Can. , 23 : 1025
   Web of Science ®Google Scholar
• Bloomfield , D. K. 1962 . Quantitative analysis of complex mixtures of steroids and bile acids . Anal. Chem. , 34 : 737
   Google Scholar
• Bloor , W. R. and Knudson , A. 1916 . Determination of cholesterol . J. Biol. Chem. , 27 : 107
   Google Scholar
• Bloor , W. R. and Snider , R. H. 1934 . Phospholipid content and activity in muscle . J. Biol. Chem. , 107 : 459
   Google Scholar
• Bondjers , G. and Bjorkerud , S. 1971 . Fluorometric determination of cholesterol and cholesteryl ester in tissue on the nanogram level . Anal. Biochem. , 42 : 363
   PubMed Web of Science ®Google Scholar
• Boucrot , Ph. 1971 . A new method for microdetermination by a combination of isotope dilution and GLC. Application to cholesterol . J. Chromatogr. Sci. , 9 : 32
   Google Scholar
• Bramell , W. S. 1972 . Report on eggs and egg products . J. Assoc. Off. Anal. Chem. , 55 : 259
   Google Scholar
• 20a. Brown, H. B., personal communication.
   Google Scholar
• Brown , H. B. and Farrad , M. 1966 . Pitfalls in constructing a fat‐controlled diet . J. Am. Diet. Assoc. , 49 : 303
   Google Scholar
• Cavanaugh , D. J. and Glick , D. 1952 . Determination of cholesterol in microgram quantities of tissues . Anal. Chem. , 24 : 1839
   Google Scholar
• Chiamori , N. and Henry , R. J. 1959 . Study of the ferric chloride method for determination of total cholesterol and cholesterol esters . Am. J. Clin. Pathol. , 31 : 305
   PubMed Web of Science ®Google Scholar
• Clarenburg , R. , Kim Chung , I. A. and Wakefield , L. M. 1971 . Reducing the egg cholesterol level by including emulsified sitosterol in standard chick diet . J. Nutr. , 101 : 289
   PubMed Web of Science ®Google Scholar
• Committee on Nutrition of the American Heart Association . 1974 . Diet and coronary heart disease . Nutr. Today , 9 : 26
   Google Scholar
• Connor , W. E. 1970 . Diet and coronary heart disease . Mod. Med. , 38 : 85
   Google Scholar
• Connor, W. E., personal communication.
   Google Scholar
• 27a. Consumer and Food Economics Institute, unpublished data.
   Google Scholar
• Crawford , N. 1958 . An improved method for determination of free and total cholesterol using the ferric chloride reaction . Clin. Chim. Acta , 3 : 357
   PubMed Web of Science ®Google Scholar
• Criner , P. E. and Feeley , R. M. 1972 . Evaluating analytical data on cholesterol in foods . J. Am. Diet. Assoc. , 61 : 115
   Google Scholar
• Cunningham , D. L. , Kreuger , W. F. , Fanguy , R. C. and Bradley , J. W. 1974 . Preliminary results of biodirectional selection for yolk cholesterol level in laying hens . Poult. Sci. , 53 : 384
   PubMed Web of Science ®Google Scholar
• Daghir , N. J. and Balloun , L. S. 1961 . Influence of fats and choline on liver and serum cholesterol in the chick . Poult. Sci. , 40 : 1712
   Google Scholar
• Daghir , N. J. and Tannous , R. I. 1965 . Comparative nutritional value of corn oil and olive oil for growing chicks . Poult. Sci. , 44 : 697
   Google Scholar
• Dam , H. 1934 . Concerning the distribution of cholesterol and cholesterol esters between fat and plasma in cow's milk . Biochem. Z. , 270 : 112
   Google Scholar
• Del Vecchio , A. , Keys , A. and Anderson , J. T. 1955 . Concentration and distribution of cholesterol in muscle and adipose tissue . Proc. Soc. Exp. Biol. Med. , 90 : 449
   Google Scholar
• De Man , J. M. 1964 . The free and ester cholesterol content of milk and dairy products . Z. Ernaeh. , 5 : 1
   Google Scholar
• Denis , W. and Minot , A. S. 1918 . Cholesterol in milk . J. Biol. Chem. , 36 : 59
   Google Scholar
• Dobbins , E. V. , Gofman , H. F. , Jones , H. C. , Lyon , L. and Young , C. 1951 . The Low Fat, Low Cholesterol Diet , Garden City, N.Y. : Doubleday .
   Google Scholar
• Domenico , F. 1940 . The influence exerted by lecithin and by cholesterol on the mammary section and the chemical composition of milk. . Arch. Fisiol. , 39 : 431
   Google Scholar
• Duthie , A. H. and Atherton , H. V. 1970 . One‐stage solvent system and one‐dimensional thin‐layer chromatographic separation of milk simple lipid classes on 20 × 20 cm plates. . J. Chromatogr. , 51 : 319
   Google Scholar
• Edwards , C. H. , Edwards , D. A. and Godsend , E. L. 1964 . Tomatine and digitonin as precipitating agents in the estimation of cholesterol . Ami. Chem. , 36 : 420
   Google Scholar
• Eisner , J. , Wong , N. P. , Firestone , D. and Bond , J. 1962 . Gas chromatography of unsaponifiable matter. I. Butter and margarine sterols . J. Assoc. Off, Anal. Chem. , 45 : 337
   Google Scholar
• Ellis , G. W. and Gardner , J. A. 1912 . The origin and destiny of cholesterol in the animal organisms . Proc. R. Soc. London, Ser. B. , 85 : 385
   Google Scholar
• Embden , G. and Lawaczeck , H. 1923 . Studies on the cholesterol content of various rabbit muscles . Z. Physiol. Chem. , 125 : 199
   Google Scholar
• Ettinger , C. L. , Malonoski , A. J. and Kirschenbaum , H. 1965 . Detection and estimation of animal fats in vegetable oils by gas chromatography . J. Assoc. Off. Anal. Chem. , 48 : 1186
   Google Scholar
• Evans , R. J. , Baudemer , S. L. and Davidson , J. A. 1967 . Lipids and fatty acids in fresh and stored shell eggs . Poult. Sci. , 46 : 151
   Web of Science ®Google Scholar
• Faush , H. D. , Richmond , R. and Anderson , T. A. 1968 . Influence of fasting on body composition and tissue cholesterol of swine . J. Anim. Sci. , 27 : 1273
   Google Scholar
• Feeley , R. , Criner , P. E. and Watt , B. K. 1972 . Cholesterol content of foods . J. Am. Diet. Assoc. , 61 : 134
   PubMedGoogle Scholar
• Fisher , H. and Brown , M. L. 1973 . Fat and cholesterol content of domestic and imported cheeses . N. J. Agric. Exp. Stn. Bull , : 832A
   Google Scholar
• Foldes , F. F. and Wilson , B. C. 1950 . Determination of cholesterol. Adaptation of the Schoenheimer‐Sperry method to photoelectric instruments . Anal. Chem. , 22 : 1210
   Web of Science ®Google Scholar
• Fox , F. W. and Gardner , J. A. 1923 . The sterol content of cow's milk. XIII . Biochem. J. , 17 : 94
   Google Scholar
• Fox , F. W. and Gardner , J. A. 1924 . The cholesterol content of human milk. XVI . Biochem. J. , 18 : 127
   Google Scholar
• Galli , G. and Paoletti , E. G. 1967 . Separation of cholesterol‐desmosterol acetates by thin‐layer and column chromatography on silica gel G‐silver nitrate . Lipids, , 2 : 72
   Google Scholar
• Garcia , M. D. , Lovern , J. A. and Olley , J. 1956 . The lipids of fish. The lipids of cod flesh . Biochem. J. , 62 : 99
   Google Scholar
• Gardner , J. A. and Williams , M. 1921 . Sources of error in the estimation of cholesterol in tissue fats . Biochem. J. , 15 : 371
   Google Scholar
• Geiger , E. J. 1949 . Cholesterol content of canned fish . JAMA , 139 : 194
   Google Scholar
• Germs , A. C. 1972 . Total cholesterol content of various weight categories of eggs . Z. Lebensm. Unters. Forsch. , 149 : 204
   Google Scholar
• Glick , D. , Fell , B. F. and Sjlin , K. E. 1964 . Spectrophotometric determination of nanogram amounts of total cholesterol in microgram quantities of tissue or microliter volumes of serum . Anal. Chem. , 36 : 1119
   Google Scholar
• Goodman , J. R. , Jarnagin , L. P. , Meir , R. M. and Shonley , I. A. 1963 . Determination of free and esterfied cholesterol by a modified digitonin‐anthrone method . Anal. Chem. , 35 : 760
   Google Scholar
• Gordon , H. T. 1951 . Indirect colorimetric micro‐oxidimetry of organic compounds . Anal. Chem. , 23 : 1853
   Google Scholar
• Gray , G. M. and Macfarlane , M. G. 1961 . Composition of phospholipids of rabbit, pigeon and trout muscle and various pig tissues . Biochem. J. , 81 : 480
   Google Scholar
• Gregorowicz , Z. and Sliwiok , J. 1970 . Application of new fuchsine for quantitative determination of organic substances in thin‐layer chromatography . Microchem. J. , 15 : 545
   Google Scholar
• Grunwald , C. 1969 . Analysis of free phytosterols by gas chromatography using liquid phase OV‐101 . J. Chromatogr. , 44 : 173
   Google Scholar
• Haenni , E. O. 1941 . A new method for the determination of cholesterol and its application to the estimation of the egg content of alimentary pastes . J. Assoc. Off. Agric. Chem. , 24 : 119
   Google Scholar
• Hanel , H. K. and Dam , H. 1955 . Determination of small amounts of total cholesterol by the Tschugaeff reaction with a note on the determination of Iathosterol. . Acta Chem. Scand. , 9 : 677
   Google Scholar
• Harris , P. A. and Harris , K. L. 1974 . Quantitative GLC analysis of plasma cholesterol . J. Pharm. Sci. , 63 : 781
   Google Scholar
• Harris , P. C. and Wilcox , F. H. 1963 . Studies on egg yolk cholesterol. III, Effects of dietary cholesterol . Poult. Sci. , 42 : 186
   Web of Science ®Google Scholar
• Harris , P. C. and Wilcox , F. H. 1963 . Studies of egg yolk cholesterol. II . Poult. Sci. , 42 : 182
   Web of Science ®Google Scholar
• Hartung , T. E. , Johnson , F. and Froning , G. W. 1973 . Composition of turkey meat. II. B‐vitamin and cholesterol content as influenced by sex and age . Poult. Sci. , 52 : 2036
   Google Scholar
• Hensel , G. and Wurziger , J. 1969 . Über Krabbenerzeugnisse unter besonderer Berücksichtigung von Krabben in Gelle . Arch. Lebensmittelhyg. , 20 : 252
   Google Scholar
• Hewitt , T. E. and Pardue , H. L. 1973 . Kinetics of the cholesterol‐sulfuric acid reaction. A fast kinetic method for serum cholesterol . Clin. Chem. , 19 : 1128
   PubMedGoogle Scholar
• Hirsch , J. and Ahrens , E. W. 1958 . The separation of complex lipid mixtures by the use of silicic acid chromatography . J. Biol. Chem. , 233 : 311
   PubMed Web of Science ®Google Scholar
• Homer , D. R. and Virtanen , A. I. 1967 . Cow's milk cholesterol‐Studies on the milk of cows on normal and protein‐free feeds . Milchwissenschaft. , 22 : 1
   Google Scholar
• Hood , R. L. and Allen , E. 1971 . Influence of sex and postmortem aging on intramuscular and subcutaneous bovine lipids . J. Food Sci. , 36 : 786
   Web of Science ®Google Scholar
• Hotta , K. 1923 . Über chemische Unterschiede Zwischen Skelettmuskeln des Kalbes and des Rindes . Z. Physiol. Chem. , 125 : 220
   Google Scholar
• Huang , T. C. , Wefler , V. and Raftery , A. 1963 . Determination of total and esterified cholesterol with tomatine . Anal. Chem. , 35 : 1757
   Google Scholar
• Hunter , M. O. , Knouff , R. A. and Brown , J. B. 1945 . The micro determination of tissue lipids . Ohio J. Sci. , 45 : 47
   Google Scholar
• Hutagalung , R. I. , Cromwell , G. L. , Hays , V. W. and Chang , C. H. 1969 . Effect of dietary fat, protein, cholesterol, and ascorbic acid on performance, serum tissue cholesterol levels and serum lipid levels of swine . J. Anim. Sci. , 29 : 700
   PubMed Web of Science ®Google Scholar
• Idler , D. R. and Baumann , C. A. 1953 . Skin sterols. III. Sterol structure and the Liebermann‐Burchard reaction . J. Biol. Chem. , 203 : 389
   Google Scholar
• Idler , D. R. and Bitneis , I. 1958 . Biochemical studies on sockeye salmon during spawning migration. II. Cholesterol, fat, protein, and water in the flesh of standard fish . Can. J. Biochem. Physiol. , 36 : 793
   Google Scholar
• Idler , D. R. and Fagerlund , U. H. M. 1955 . Marine sterols. I. Isolation of 24‐methylene‐cholesterol from mollusca . J. Am. Chem. Soc. , 77 : 4142
   Google Scholar
• Idler , D. R. and Safe , L. M. 1972 . Separation of steryl acetates by silver ion chromatography . Steroids. , 19 : 315
   Google Scholar
• Idler , D. R. , Tamura , T. and Wainai , T. 1964 . Seasonal variations in the sterol fat and unsaponifiable components of scallop muscle . J. Fish. Res. Board Can. , 21 : 1035
   Google Scholar
• Ishikawa , T. T. , MacGee , J. , Morrison , J. A. and Glueck , C. J. 1974 . Quantitative analysis of cholesterol in 5 to 20 μl of plasma . J. Lipid Res. , 15 : 236
   Google Scholar
• Johansen , R. G. and Voris , S. S. 1971 . GLC determination of cholesterol as an index of egg content in baked goods . Cereal Chem. , 48 : 576
   Google Scholar
• Jurgens , M. H. and Peo , E. R. 1970 . Influence of dietary supplements of cholesterol and vitamin D on certain components of the blood and body of growing‐finishing swine . J. Anim. Sci. , 30 : 894
   Google Scholar
• Kabara , J. J. and McLaughlin , J. T. 1961 . A micro dibromide method for purifying radioactive cholesterol . J. Lipid Res. , 2 : 283
   Web of Science ®Google Scholar
• Katada , M. , Zama , K. and Igarashi , H. 1960 . Lipids of the muscle of tuna, Thynnus orientalis. VII. Distribution of lipids in dark‐colored muscle, ordinary muscle, and several other tissues . Bull. Jpn. Soc. Sci. Fish. , 26 : 425
   Google Scholar
• Kaucher , M. , Galbraith , H. , Button , V. and Williams , H. 1943 . The distribution of lipids in animal tissue . Arch. Biochem. , 3 : 203
   Google Scholar
• Kelsey , F. E. 1939 . Determination of cholesterol . J. Biol. Chem. , 127 : 15
   Google Scholar
• Kritchevsky , D. and Tepper , S. A. 1961 . The free and ester sterol content of various foodstuffs . J. Nutr. , 74 : 441
   Web of Science ®Google Scholar
• Kritchevsky , D. , Tepper , S. A. , DiTullio , N. and Holmes , W. 1967 . The sterols of seafood . J. Food Sci. , 32 : 64
   Web of Science ®Google Scholar
• Kunz , F. 1973 . Separation of “neutral”; lipids, particularly of all classes of partial glycerides by one‐dimensional thin‐layer chromatography . Biochim. Biophys. Acta , 296 : 331
   Google Scholar
• LaCroix , D. 1969 . Extraction of plant sterols from adulterated butter oil using a digitonin‐impregnated celite column . J. Assoc. Off. Anal Chem. , 52 : 600
   Google Scholar
• LaCroix , D. , Feeley , R. M. , Wong , N. P. and Alford , J. A. 1972 . Comparison of two extraction methods for determination of cholesterol in dairy products . J. Assoc. Off. Anal Chem. , 55 : 972
   PubMedGoogle Scholar
• LaCroix , D. E. , Mattingly , W. A. , Wong , N. P. and Alford , J. A. 1973 . Cholesterol, fat and protein in dairy products . J. Am. Diet. Assoc. , 62 : 275
   Google Scholar
• Lampert , L. M. 1930 . Cholesterol as a measure of egg yolk in milk products . Ind. Eng. Chem. Anal. Ed., , 2 : 159
   Google Scholar
• Lange , W. 1950 . Cholesterol, phytosterol, and tocopherol content of food products and animal tissue . J. Am. Oil Chem. Soc. , 27 : 414
   Web of Science ®Google Scholar
• Laskowski , K. 1961 . Cholesterol content of eggs, milk and cream in Poland . Pr. Inst. Prz. Mlecz. , 8 : 21
   Google Scholar
• Laskowski , K. 1963 . Study of the content of unsaturated fatty acids, cholesterol and lipid in milk and margarine . Pr. Inst. Prz. Mlecz. , 9 : 1
   Google Scholar
• Lawaczeek , H. 1923 . Cholesterol content of different rabbit muscles . Z. Physiol Chem. , 125 : 199
   Google Scholar
• Lawaczeek , H. 1923 . Cholesterol content of different muscles . Z. Physiol Chem. , 125 : 210
   Google Scholar
• Leach , B. E. , Rainey , N. H. and Pallansch , M. J. 1966 . Physical and chemical analyses of film formed in air‐water interface on reconstruction of whole milk powders . J. Dairy Sci. , 49 : 1465
   Google Scholar
• Leveille , G. A. and Fisher , H. 1958 . Observation on lipid utilization in hens fed vegetable and animal fat supplemented diets . Poult. Sci. , 37 : 658
   Web of Science ®Google Scholar
• Levine , J. B. and Zak , B. 1964 . Automated determination of total serum cholesterol . Clin. Chim. Acta. , 10 : 381
   PubMed Web of Science ®Google Scholar
• Levy , R. I. and Ernst , N. 1973 . “ Diet, hyperlipidimiea and atherosclerosis ” . In Modem Nutrition in Health and Disease: Dietotherapy, , 5th ed. , Edited by: Goodhart , R. S. and Shils , M. E. Philadelphia : Lea and Febiger .
   Google Scholar
• Lovern , J. A. , Olley , J. and Watson , H. A. 1959 . Changes in the lipids of cod during storage in ice . J. Sci. Food Agric. , 10 : 327
   Google Scholar
• Lyaskovskaya , Y. N. and Kelman , L. F. 1967 . Free and esterified cholesterol levels in the muscle tissue of slaughtered animals . Vopr. Pitan. , 26 : 62
   Google Scholar
• Manasterski , A. and Zak , B. 1973 . Spectrophotometric study of bromide interference in a cholesterol reaction . Microchem. J. , 18 : 18
   Google Scholar
• Mann , G. V. 1961 . A method for measurement of cholesterol in blood serum . Gin. Chem. , 7 : 275
   Google Scholar
• Marion , J. E. and Woodroof , J. G. 1965 . Lipid fractions of chicken broiler tissues and their fatty acid composition . J. Food Sci. , 30 : 38
   Web of Science ®Google Scholar
• Martenson , E. H. 1962 . Investigation of factors affecting the Liebermann‐Burchard cholesterol reaction . Scand. J. Clin. Lab. Invest. Suppl. , 15 : 164
   Google Scholar
• Martin , W. G. , Tattrie , N. H. and Cook , W. H. 1963 . Lipid extraction and distribution studies of egg yolk lipoproteins . Can. J. Biochem. Physiol. , 41 : 157
   Google Scholar
• McCarthy , M. J. , Kuksis , A. and Beveridge , J. M. R. 1964 . Composition of molecular distillates of butter oil; isolation and identification of components other than glycerides . J. Lipid Res. , 5 : 609
   Google Scholar
• McGinnis , C. H. and Ringer , R. K. 1963 . The effect of a quartenary ammonium anion exchange resin on plasma and egg yolk cholesterol in the laying hen. . Poult. Sci. , 42 : 394
   Web of Science ®Google Scholar
• Mickelberry , W. C. , Rogler , J. C. and Stadelman , W. J. 1964 . Effects of dietary fats on broiler tissues . J. Am Diet. Assoc. , 45 : 234
   Google Scholar
• Mickelberry , W. C. , Rogler , J. C. and Stadelman , W. J. 1966 . The influence of dietary fat and environmental temperature upon chick growth and carcass composition . Poult. Sci. , 45 : 313
   PubMed Web of Science ®Google Scholar
• Mickelsen, O., personal communication.
   Google Scholar
• Moline , C. and Barron , E. J. 1969 . The determination of serum lipids . Clin. Biochem. , 2 : 321
   Google Scholar
• Mulder , H. and Zuidhof , T. A. 1958 . The sugar layers of milk fat globules. IV. The cholesterol content (free and esterified cholesterol) of the surface layers . Neth. Milk Dairy J. , 12 : 173
   Google Scholar
• Munsey , V. E. 1954 . Report on egg content of noodles . J. Assoc. Off. Agric. Chem. , 37 : 408
   Google Scholar
• Namiki , S. 1933 . Uber den Cholesterin Gehalt der Muskulature von Fischen . J. Biochem., (Japan) , 18 : 163
   Google Scholar
• Nataf , B. , Mickelsen , O. , Keys , A. and Peterson , W. E. 1948 . The cholesterol content of cow's milk . J. Nutr. , 36 : 495
   Google Scholar
• Negrin , A. 1969 . Measurement of cholesterol in tissues with phospho‐6‐tungstic‐12‐molybdic acid . Clin. Chem. , 15 : 829
   PubMedGoogle Scholar
• Neudoerffer , T. S. and Lea , C. H. 1968 . Effects of dietary fat on the amounts and proportions of the individual lipids in the turkey muscle . Br. J. Nutr. , 22 : 115
   Google Scholar
• Nichols , E. L. , Marion , W. W. and Balloun , S. L. 1962 . Influence of triparanol on growth, egg production and cholesterol metabolism of chickens . Poult. Sci. , 41 : 1494
   Web of Science ®Google Scholar
• Nix , D. F. , Thornton , E. J. , Washburn , K. W. and Marks , H. L. 1974 . The influence of moulting on yolk cholesterol level . Poult. Sci. , 53 : 412
   Web of Science ®Google Scholar
• Nockles , C. F. 1973 . The influence of feeding ascorbic acid and sulfate on egg production and on cholesterol content of certain tissues of the hen . Poult. Sci. , 52 : 373
   Google Scholar
• Norris , R. , Gray , I. K. , McDowell , A. K. R. and Dolby , R. M. 1971 . The chemical composition and physical properties of fractions of milk fat obtained by a commercial fractionation process . J. Dairy Res. , 38 : 179
   Web of Science ®Google Scholar
• Novotny , M. , Segura , R. and Zlatkis , A. 1972 . High‐temperature gas‐chromatographic separations using glass capillary columns and carborane stationary phases . Anal. Chem. , 44 : 9
   Google Scholar
• Horwitz , W. , ed. 1970 . Official Methods of Analysis, , 11th ed. , Washington, D.C. : J. Association Off. Agric. Chem. .
   Google Scholar
• Okey , R. 1930 . A micro method for the estimation of cholesterol by oxidation of the digitonide . J. Biol. Chem. , 88 : 367
   Google Scholar
• Okey , R. 1945 . Cholesterol content of foods . J. Am. Diet. Assoc. , 21 : 341
   Google Scholar
• Olley , J. and Lovern , J. A. 1954 . The lipids of fish. V. The lipids remaining in the flesh of haddock after extraction by acetone‐ethanol‐ether . Biochem. J. , 57 : 610
   Google Scholar
• Parekh , A. C. and Jung , D. H. 1970 . Cholesterol determination with ferric acetate‐uranium acetate and sulfuric acid‐ferrous sulfate reagents . Anal. Chem. , 42 : 1423
   Web of Science ®Google Scholar
• Parodi , P. W. 1973 . The sterol content of milk fat, animal fats, margarines, and vegetable oils. . Aust. J. Dairy Technol. , 28 : 135
   Google Scholar
• Pascagoula Fisheries Station, Bureau of Commercial Fisheries, personal communication, 1963.
   Google Scholar
• Pearson , S. , Stern , S. and McGavack , T. H. 1953 . A rapid accurate method for the determination of total cholesterol in serum . Anal Chem. , 25 : 813
   Web of Science ®Google Scholar
• Pentz , I. and McArthur , E. M. 1951 . A modified digitonin reagent for the determination of cholesterol . J. Lab. Clin. Med , 37 : 150
   Google Scholar
• Pihl , A. 1952 . Cholesterol studies. I. The cholesterol content of foods . Scand. J. Clin. Lab. Invest. , 4 : 115
   PubMed Web of Science ®Google Scholar
• Pokorny , S. , Coupek , J. , Luan , N. T. and Pokorny , J. 1973 . Gel chromatogiaphic separation of unsaponifiable fractions in fats and oils . J. Chromatogr. , 84 : 319
   Google Scholar
• Privett , O. S. , Dougherty , K. A. and Castell , J. D. 1971 . Quantitative analysis of lipid classes . Am. J. Clin. Nutr. , 24 : 1265
   Google Scholar
• Procter , B. E. , Miller , S. A. , Wick , E. L. , Pariser , E. R. , Sapers , G. M. and Solberg , M. 1961 . The nutritive value of Maine sardines. I. Chemical composition. . J. Food Sci. , 26 : 283
   Google Scholar
• Rangachar , T. R. S. , Setty , S. V. S. and Hegde , R. 1970 . Cholesterol content in eggs of chicken and duck . Mysore J. Agric. Sci. , 4 : 146
   Google Scholar
• Reiser , R. , Sorrels , M. F. and Williams , M. C. 1959 . Influence of high levels of dietary fats and cholesterol on atherosclerosis and lipid distribution in swine . Or. Res. , 7 : 833
   Google Scholar
• Rhodes , D. N. 1959 . Interference by polyunsaturated fatty acids in the determination of cholesterol . Biochem. J. , 71 : 26
   Google Scholar
• Rinehart , R. K. , Delaney , S. E. and Sheppard , H. 1962 . Determination of cholesterol as the tomatinide by using the iron reagent . J. Lipid Res. , 3 : 383
   Google Scholar
• Roberts , L. 1968 . Fluorometric determination of sterols as cholesterol in egg noodles, Collaborative study . J. Assoc. Off. Anal. Chem. , 51 : 1220
   Google Scholar
• Rosenthal , H. L . 1957 . A stable reagent for the determination of cholesterol . J. Lab. Clin. Med. , 50 : 318
   Google Scholar
• Rudel , L. L. and Morris , M. D. 1973 . Determination of cholesterol using o‐phthalaldehyde . J. Lipid Res. , 14 : 364
   PubMed Web of Science ®Google Scholar
• Sapers , G. M. and Solberg , M. 1961 . The nutritional value of marine sardines. I. Chemical composition . J. Food Sci. , 26 : 283
   Google Scholar
• Schoenheimer , R. and Sperry , W. M. 1934 . A micromethod for the determination of free and combined cholesterol . J. Biol. Chem. , 106 : 745
   Google Scholar
• Schwartz , D. P. , Brewington , C. R. and Burgwald , L. H. 1967 . Rapid quantitative procedure for removing cholesterol from butter fat . J. Lipid Res. , 8 : 54
   PubMed Web of Science ®Google Scholar
• Seher , A. and Homberg , A. E. 1968 . The investigation of sterol mixtures with the help of thin‐layer chromatography . Fette Seifen Anstrichm. , 70 : 481
   Google Scholar
• Sgoutas , D. 1966 . The lipids in normal chicken liver . Can. J. Biochem. , 44 : 763
   Google Scholar
• Skipski , V. P. , Good , J. , Barclay , M. and Reggio , R. B. 1968 . Quantitative analysis of simple lipid classes by thin‐layer chromatography . Biochim. Biophys. Acta , 152 : 10
   PubMedGoogle Scholar
• Sperry , W. M. and Brand , F. C. 1943 . The colorimetric determination of cholesterol . J. Biol. Chem. , 150 : 315
   Google Scholar
• Sperry , W. M. and Webb , M. 1950 . A revision of the Schoenheimer‐Sperry method for cholesterol determination . J. Biol. Chem. , 187 : 97
   PubMed Web of Science ®Google Scholar
• Sperry , W. M. and Webb , M. 1963 . Quantitative isolation of sterols . J. Lipid Res. , 4 : 221
   PubMedGoogle Scholar
• Standal , B. , Bassett , D. and Thomas , M. 1970 . Fatty acids, cholesterol, and proximate analysis of some ready‐to‐eat food . J. Am. Diet. Assoc. , 56 : 392
   Google Scholar
• Steger , H. 1961 . Uber den Cholesteringehalt der Milch unser Haustiere im verlaufe der Laktation und Dessen Bestimmung . Arch. Tierz. , 4 : 199
   Google Scholar
• Stewart , D. W. 1923 . Some observations with regard to unsaponifiable matter and sterols of edible fats . Analyst , 48 : 155
   Google Scholar
• Stromer , M. H. , Goll , D. E. and Roberts , J. H. 1966 . Cholesterol in subcutaneous and intramuscular lipid depots from bovine carcasses of different maturity and fatness . J. Anim. Sci. , 25 : 1145
   Google Scholar
• Szent Gyorgyi , A. 1923 . The gravimetric determination of cholesterol . Biochem. J. , 136 : 107
   Google Scholar
• Tamura , T. , Wainai , T. , Truscott , B. and Idler , D. R. 1964 . Isolation of 22‐dehydrocholesterol from scallop . Can. J. Biochem. , 42 : 1331
   Google Scholar
• Tarjan , R. , Kramer , M. , Szoke , K. , Lindner , R. , Szarvas , T. and Dworschak , E. 1965 . The effect of different factors on the composition of human milk. VIII. The composition of human milk during lactation . Nutr. Dieta. , 1 : 136
   Google Scholar
• Tattrie , N. H. 1972 . Isolation and identification of egg yolk cholesterol esters . Can. J. Biochem. , 50 : 1414
   PubMedGoogle Scholar
• Teichman , R. J. , Takei , G. H. and Cummins , J. M. 1974 . Detection of fatty acids, fatty aldehydes, phospholipids, glycolipids and cholesterol on thin‐layer chromatograms stained with malachite green . J. Chromatogr. , 88 : 425
   PubMed Web of Science ®Google Scholar
• Terrell , R. N. , Suess , G. G. and Bray , R. W. 1969 . Influence of sex, live‐weight, and anatomical location on bovine lipids. II. Lipid components and subjective scores of six muscles . J. Anim. Sci. , 28 : 454
   Google Scholar
• Thompson , M. P. , Brunner , J. R. , Lindquist , K. and Stine , C. M. 1961 . Lipid components of the fat globule membrane . J. Dairy Sci. , 44 : 1589
   Google Scholar
• Thompson , M. H. 1964 . Cholesterol content of various species of shellfish. I. Method of analysis and preliminary survey of variables . Fish. Ind. Res. , 2 : 11
   Google Scholar
• Thorpe , C. W. , Pohland , L. and Firestone , D. 1969 . Thin‐layer and gas‐liquid chromatography of cholesterol in fats and oils. I. Development of method . J. Assoc. Off. Anal. Chem. , 52 : 774
   Google Scholar
• Thorpe , C. W. 1970 . Comparison of separation procedures for identification of oils by gas chromatography . J. Assoc. Off. Anal. Chem. , 53 : 623
   Google Scholar
• Toepfer , E. and Morgareidge , K. 1971 . Effect of malathion on nutrient composition of eggs and flavor of meat from layinghens . J. Food Sci. , 36 : 511
   Google Scholar
• Tsuchiya , F. 1972 . Steroids of milk lipids . Nippion Chikusan. Gakkai‐Ho , 43 : 506
   Google Scholar
• Tu , C. , Powrie , W. D. and Fennema , O. 1967 . Free and esterified cholesterol content of animal muscles and meat products . J. Food Sci. , 32 : 30
   Google Scholar
• Tu , C. , Powrie , W. D. and Fennema , O. 1970 . Steroids in egg yolk . J. Food Sci. , 35 : 601
   Google Scholar
• Turk , D. E. and Barnett , B. D. 1971 . Cholesterol content of market eggs . Poult. Sci. , 50 : 1303
   PubMed Web of Science ®Google Scholar
• Vahouny , G. V. , Borja , C. R. , Mayer , R. M. and Treadwell , C. R. 1960 . A rapid quantitative method for determination of total and free cholesterol with anthrone reagent . Anal. Biochem. , 1 : 371
   PubMed Web of Science ®Google Scholar
• Van Dam , M. J. , de Kleuver , C. J. and de Heus , J. G. 1960 . Thin‐layer chromatography of weakly polar steroids . J. Chromatogr. , 4 : 26
   Google Scholar
• VandenHeuvel , W. J. A. , Sweeley , C. C. and Horning , E. C. 1960 . Separation of steroids by gas chromatography . J. Am. Chem. Soc. , 82 : 3481
   Web of Science ®Google Scholar
• VandenHeuvel , W. J. A. and Horning , E. C. 1962 . A study of retention time relationships in gas chromatography in terms of the structure of steroids . Biochim. Biphys. Acta , 64 : 416
   PubMedGoogle Scholar
• Wacker , L. and Beck , K. F. 1921 . Investigation of the cholesterol content of women's and cow's milk . Z. Kindermilch. , 21 : 288
   Google Scholar
• Wainai , T. , Tamura , T. , Truscott , B. and Idler , D. R. 1964 . The application of gas chromatography to the identification of sterols of scallop . J. Fish. Res. Board Can. , 21 : 1543
   Google Scholar
• Warburton , D. Quantitative Determination of Total Cholesterol in Body Fluids. . U.S. Patent Appl. 255, 476 . 1972 .
   Google Scholar
• Washburn , K. W. and Nix , D. F. 1974 . Genetic basis of yolk cholesterol content . Poult. Sci. , 53 : 109
   PubMed Web of Science ®Google Scholar
• Watt , B. K. and Merrill , A. L. I. 1963 . Composition of Foods ‐ ‐ raw, processed, prepared , U.S. Department of Agriculture Handbook No. 8 (revised)
   Google Scholar
• Weiss , J. F. , Naber , E. C. and Johnson , R. M. 1964 . Effect of dietary fat and other factors on egg yolk cholesterol. I. The “cholesterol”; content of egg yolk as influenced by dietary unsaturated fat and the method of determination . Arch. Biochem. Biophys. , 105 : 521
   PubMed Web of Science ®Google Scholar
• Weigensberg , R. J. and McMillan , G. C. 1959 . Ultraviolet spectrophotometric method for the determination of cholesterol . Am. J. Clin. Pathol. , 31 : 16
   PubMedGoogle Scholar
• Windaus , A. Z. 1910 . A gravimetric digitonin method for the determination of cholesterol . Z. Physiol. Chem. , 65 : 110
   Google Scholar
• Wulfert , K. 1947 . Quantitative colorimetrical determination of certain sex hormones belonging to the steroid group . Acta Chem. Scand. , 1 : 818
   Google Scholar
• Wurziger , J. and Hensel , G. 1970 . Shrimp and shrimp products. . Arch. Lebensmittelhyg. , 21 : 132
   Google Scholar
• Wycoff , H. D. and Parsons , J. 1957 . Chromatographic microassay for cholesterol and cholesterol esters . Science , 125 : 347
   Google Scholar
• Yamazoe , Y. 1960 . Determination of cholesterol in fish meat with sapon. . Kagaku., (Tokyo) , 30 : 258
   Google Scholar
• Zak , B. 1965 . Total and free cholesterol. . Stand. Methods, of Clin. Chem. , 5 : 79
   Google Scholar
• Zak , B. and Epstein , E. 1961 . A study of several color reactions for the determination of serum cholesterol . Clin. Chem. Acta , 6 : 72
   Google Scholar
• Zlatkis , A. , Zak , B. and Boyle , A. J. 1953 . A new method for the direct determination of serum cholesterol . J. Lab. Clin. Med. , 41 : 486
   PubMed Web of Science ®Google Scholar
• Zlatkis , A. and Zak , B. 1969 . Study of a new cholesterol reagent . Anal. Biochem. , 29 : 143
   PubMed Web of Science ®Google Scholar
• Zurkowski , P. 1964 . A rapid method for cholesterol determination with a single reagent . Clin. Chem. , 10 : 451
   PubMed Web of Science ®Google Scholar
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   Google Scholar