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Original Articles

Whey protein concentrates and isolates: Processing and functional properties

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Pages 431-476 | Published online: 29 Sep 2009

References

  • Eigel , W. N. , Butler , J. E. , Ernstrom , C. A. , Farrell , H. M. Jr. , Harwalkar , V. R. , Jenness , R. and Whitney , R. McL. 1984 . Nomenclature of proteins of cow's milk: fifth revision . J. Dairy Sci. , 67 : 1599
  • Morr , C. V. 1975 . Chemistry of milk proteins in milk processing . J. Dairy Sci. , 58 : 977
  • Morr , C. V. 1985 . Composition, physico‐chemical and functional properties of reference whey protein concentrates . J. Food Sci. , 50 : 1406
  • Morr , C. V. and Richter , R. L. 1988 . “ Chemistry of processing ” . In Fundamentals of Dairy Chemistry , Edited by: Wong , N. P. 739 New York : Van Nostrand Reinhold .
  • de Wit , J. N. 1984 . Functional properties of whey proteins in food systems . Neth. Milk Dairy J. , 38 : 71
  • de Wit , J. N. 1989 . “ Functional properties of whey proteins ” . In Developments in Dairy Chemistry , Edited by: Fox , P. F. Vol. 4 , 285 New York : Elsevier Applied Science .
  • de Wit , J. N. 1989 . “ The use of whey protein products ” . In Developments in Dairy Chemistry , Edited by: Fox , P. F. Vol. 4 , 323 New York : Elsevier Applied Science .
  • Pour‐El , A. 1981 . “ Protein functionality: classification, definition, and methodology ” . In Protein Functionality in Foods , Edited by: Cherry , J. P. 1 Washington, D.C. : Am. Chem. Soc. .
  • Kinsella , J. E. 1982 . “ Relationships between structure and functional properties of food proteins ” . In Food Proteins , Edited by: Fox , P. F. and Condon , J. J. 51 New York : Applied Science .
  • Kinsella , J. E. 1984 . Milk proteins: physicochemical and functional properties . CRC Crit. Rev. Food Sci. Nutr. , 21 : 197
  • Kinsella , J. E. , Whitehead , D. M. , Brady , J. and Bringe , N. A. 1989 . “ Milk proteins’, possible relationships of structure and function ” . In Developments in Dairy Chemistry , Vol. 4 , 55 New York : Elsevier Applied Science .
  • Mangino , M. E. 1984 . Physicochemical basis of whey protein functionality . J. Dairy Sci. , 67 : 2711
  • Marshall , K. R. 1982 . “ Industrial isolation of milk proteins: whey proteins ” . In Developments in Dairy Chemistry, Vol. 1 Proteins , Edited by: Fox , P. F. 339 New York : Applied Science .
  • Morr , C. V. 1982 . “ Functional properties of milk proteins and their use as food ingredients ” . In Developments in Dairy Chemistry, Vol. 1 Proteins , Edited by: Fox , P. F. 375 New York : Applied Science .
  • Morr , C. V. 1989 . “ Whey protein: manufacture ” . In Developments in Dairy Chemistry , Edited by: Fox , P. F. Vol. 4 , 245 New York : Elsevier Applied Science .
  • Schmidt , R. H. , Packard , V. S. and Morris , H. A. 1984 . Effects of processing on whey protein functionality . J. Dairy Sci. , 67 : 2723
  • Sienkiewcz , T. and Riedel , C.‐L. 1990 . Whey and Whey Utilization , Edited by: Mann , Verlag Th. Germany : Gelsenkirchen‐Buer .
  • Dybing , S. T. and Smith , D. E. 1991 . Relation of chemistry and processing procedures to whey protein functionality: a review . Cult. Dairy Prod. J. , 28 : 4
  • Zall , R. R. 1984 . Trends in whey fractionation and utilization, a global perspective . J. Dairy Sci. , 67 : 2621
  • Morr , C. V. 1984 . Production and use of milk proteins in food . Food Technol. , 38 : 39
  • Van Hoogstraeten , J. J. 1987 . “ The marketing of whey products: a review from Europe “J'ai deux amours”; ” . In Trends in Whey Utilization, Int. Dairy Fed. , 17 Belgium : Brussels .
  • Anon. 1992 . Whey Products: 1991 Utilization and Production Trends , Chicago : Am. Dairy Prod. Inst. .
  • Walstra , P. and Jenness , R. 1984 . Dairy Chemistry, and Physics , New York : John Wiley & Sons .
  • Bassette , R. and Acosta , J. S. 1988 . “ Composition of milk products ” . In Fundamentals of Dairy Chemistry , Edited by: Wong , N. P. 39 New York : Van Nostrand Reinhold .
  • Hartman , G. H. Jr. and Swanson , A. M. 1966 . Precipitation of protein from cheese whey with poly‐phosphates . J. Dairy Sci. , 49 : 697
  • Morr , C. V. , Swenson , P. E. and Richter , R. L. 1973 . Functional characteristics of whey protein concentrates . J. Food Sci. , 38 : 324
  • Melachouris , N. 1984 . Critical aspects in development of whey protein concentrate . J. Dairy Sci. , 67 : 2693
  • Maubois , J. L. , Pierre , A. , Fauquant , J. and Piot , M. 1987 . “ Industrial fractionation of main whey proteins ” . In Trends in Whey Utilization , 154 Brussels, , Belgium : Int. Dairy Fed. .
  • Morr , C. V. and Ha , E. Y. W. 1991 . Off‐flavors of whey protein concentrates: a literature review . Int. Dairy J. , 1 : 1
  • Pearce , R. J. 1987 . “ Fractionation of whey proteins ” . In Trends in Whey Utilization , 150 Brussels, Belgium : Int. Dairy Fed. .
  • Anon. 1992 . Meeting the challenge: whey and lactose opportunities . WMMB Rev. , 5 (1)
  • Singer , N. . Simplesse® all natural fat substitute and the dairy industry . Proc. Dairy Prod. Tech. Conf. . pp. 85 Chicago : Am. Dairy Prod. Inst. and Center for Dairy Res. .
  • Duxbury , D. D. 1992 . New ingredient provides healthy, functional alternative to eggs in bakery goods . Food Process. , 53 : 96
  • Duxbury , D. D. 1992 . “Super”; stabilizer versatile as fat replacer. . Food Process. , 53 : 39
  • Hugunin , A. G. 1987 . “ Applications of U.F. whey protein: developing new markets ” . In Trends in Whey Utilization , 135 Brussels, , Belgium : Int. Dairy Fed. .
  • Morr , C. V. and Foegeding , E. A. 1990 . Composition and functionality of commercial whey and milk protein concentrates and isolates: a status report . Food Technol. , 44 : 100
  • Morr , C. V. 1987 . “ Fractionation and modification of whey protein in the U.S. ” . In Trends in Whey Utilization , 145 Brussels, , Belgium : Int. Dairy Fed. .
  • Morr , C. V. 1992 . Improving the texture and functionality of whey protein concentrate . Food Technol. , 46 : 110
  • Breslau , B. R. , Goulet , J. and Cross , R. A. 1975 . Production of a crystal clear, bland tasting protein solution from cheese whey . Cult. Dairy Prod. J. , 11 : 13
  • Whitfield , F. B. 1992 . Volatiles from interactions of Maillard reactions in lipids . CRC Crit. Rev. Food Sci. Nutr. , 31 : 1
  • de Wit , J. N. , Hontelez‐Backx , E. and Adamse , E. 1988 . Evaluation of functional properties of whey protein concentrates and whey protein isolates. III. Functional properties in aqueous solution . Neth. Milk Dairy J. , 42 : 155
  • Chou , D.H.‐E. and Morr , C. V. 1979 . Protein‐water interactions and functional properties . J. Am. Oil Chem. Soc. , 56 : 53A
  • Morr , C. V. 1989 . Beneficial and adverse effects of water‐protein interactions in selected dairy products . J. Dairy Sci. , 72 : 575
  • Kinsella , J. E. 1981 . Functional properties of proteins: possible relationships between structure and function in foams. . Food Chem. , 7 : 273
  • Hermansson , A.‐M. and Åkesson , C. 1975 . Functional properties of added proteins correlated with properties of meat systems. Effect of salt on water‐binding properties of mode! meat systems . 40 : 603
  • Morr , C. V. and Josephson , R. V. 1968 . Effect of calcium, N‐ethylmaleimide and casein upon heat‐induced whey protein aggregation . J. Dairy Sci. , 51 : 1349
  • Johns , J. E. M. and Ennis , B. M. 1981 . The effect of replacement of calcium with sodium in acid whey on the functional properties of whey protein concentrate . N.Z. J. Dairy Sci. Technol. , 15 : 79
  • Townsend , R. and Gyuricsek , D. M. 1974 . Heat denatur‐ation of whey and model (milk) protein systems . J. Dairy Sci. , 57 : 1152
  • Mulvihill , D. M. and Kinsella , J. E. 1987 . Gelation characteristics of whey proteins and β‐lactoglobulin . Food Technol. , 41 : 102
  • Mangino , M. E. 1992 . Gelation of whey protein concentrates . Food Technol. , 46 : 114
  • Brandenburg , A. H. , Morr , C. V. and Welter , C. L. 1992 . Gelation of commercial whey protein concentrates: effect of removal of low‐molecular‐weight components . J. Food Sci. , 57 : 427
  • Morr , C. V. , German , B. , Kinsella , J. E. , Regenstein , J. P. , Van Buren , J. P. , Kilara , A. , Lewis , B. and Mangino , M. E. 1985 . A collaborative study to develop a standardized food protein solubility procedure . J. Food Sci. , 51 : 1715
  • Mulvihill , D. M. 1987 . “ Assessment of the functional properties of milk protein products ” . In Trends in Whey Utilization , 3 Brussels, , Belgium : Int. Dairy Fed. .
  • Patel , P. D. and Fry , J. C. 1985 . “ The search for standardized methods for assessing protein functionality ” . In Developments in Food Proteins‐5 , Edited by: Hudson , F. J. F. 299 New York : Elsevier Applied Science .
  • Phillips , L. G. , German , B. E. , O'neil , T. E. , Foegeding , E. A. , Harwalkar , V. R. , Kilara , A. , Lewis , B. A. , Mangino , M. E. , Morr , C. V. , Regenstein , J. M. , Smith , D. M. and Kinsella , J. E. 1990 . Standardized procedure for measuring foaming properties of three proteins, a collaborative study . J. Food Sci. , 55 : 1441
  • Kenkare , D. B. , Morr , C. V. and Gould , I. A. 1964 . Factors affecting the heat aggregation of proteins in selected skim milk sera . J. Dairy Sci. , 47 : 947
  • Neilsen , M. A. , Coulter , S. T. , Morr , C. V. and Rosenau , J. R. 1973 . Four factor response surface experimental design for evaluating the role of processing variables upon protein denaturation in heated whey systems . J. Dairy Sci. , 56 : 76
  • Varunsatian , S. , Watanabe , K. , Hayakawa , S. and Nakamura , R. 1983 . Effects of Ca++, Mg++ and Na+ on heat aggregation of whey protein concentrates . J. Food Sci. , 48 : 42
  • Hill , A. R. 1988 . Thermal precipitation of whey proteins . Milchwissenschaft , 43 : 565
  • Dannenberg , F. and Kessler , H. G. 1988 . Application of reaction kinetics to the denaturation of whey proteins in heated milk . Milchwissenschaft , 43 : 3
  • Dannenberg , F. and Kessler , H. G. 1988 . Thermodynamic approach to kinetics of β‐lactoglobulin denaturation in heated skim milk and sweet whey . Milchwissenschaft , 43 : 137
  • Oysun , V. G. and Alpkent , Z. 1989 . Hitzedenaturierung von Molenproteinen bei Verschiedenen pH . Milchwissenschaft , 44 : 87
  • Xiong , Y. L. 1992 . Influence of pH and ionic environment on thermal aggregation of whey proteins . J. Agric. Food Chem. , 40 : 380
  • Whitney , R. McL. , Brunner , J. R. , Ebner , K. E. , Farrell , H. M. Jr. , Josephson , R. V. , Morr , C. V. and Swaisgood , H. E. 1976 . Nomenclature of the proteins of cow's milk: fourth revision . J. Dairy Sci. , 59 : 795
  • Garoutte , C. A. , Amundson , C. H. and Ernstrom , C. A. 1985 . Residual milk coagulants in whey—their inac‐tivation and effects on ultrafiltration flux and nitrogen yields . J. Dairy Sci. , 68 : 817
  • Anon. 1989 . Chymogen™, Advanced Rennet Technology , Milwaukee, WI : Chr. Hansen's Laboratory .
  • Houlihan , A. V. and Goddard , P. A. 1991 . Analysis of a milk membrane fraction isolated from whey protein concentrates . Aust. J. Dairy Technol. , 46 : 77
  • Ebeler , S. E. , Phillips , L. G. and Kinsella , J. E. 1990 . Purification of β‐lactoglobulin: isolation of genetic variants and influence of purification method on secondary structure . Milchwissenschaft , 45 : 694
  • Swaisgood , H. E. 1982 . “ Chemistry of milk proteins ” . In Developments in Dairy Chemistry, Vol. 1 Proteins , Edited by: Fox , P. F. Vol. 1 , 1 New York : Applied Science .
  • Swaisgood , H. E. 1985 . “ Characteristics of edible fluids of animal origin: milk ” . In Food Chemistry , Edited by: Fennema , O. R. 792 New York : Marcel Dekker .
  • Godovac‐Zimmermann , J. and Braunitzer , G. 1987 . Modern aspects of the primary structure and function of β‐lactoglobulin . Milchwissenschaft , 42 : 294
  • Reddy , I. M. , Kella , N. K. D. and Kinsella , J. E. 1988 . Structural and conformational basis of the resistance of β‐lactoglobulin to peptic and chymotryptic digestion . J. Agric. Food Chem. , 36 : 737
  • de Wit , J. N. and Swinkels , G. A. M. 1980 . A differential scanning calorimetric study of the thermal denaturation of bovine β‐lactoglobulin: thermal behavior at temperatures up to 100°C . Biochim. Biophys. Acta , 624 : 40
  • McKenzie , H. A. 1971 . Milk Proteins , Edited by: McKenzie , H. A. Vol 2 , New York : Academic Press .
  • Anfinsen , C. B. and Scheraga , H. A. 1975 . Experimental and theoretical aspects of protein folding . Adv. Protein Chem. , 29 : 205
  • de Wit , J. N. 1981 . Structure and functional behavior of whey proteins . Neth. Milk Dairy J. , 35 : 47
  • Morr , C. V. 1976 . Whey protein concentrates: an update . Food Technol. , 30 : 18
  • Rüegg , M. , Moor , U. and Blanc , B. 1977 . A calorimetric study of thermal denaturation of whey protein in simulated milk ultrafiltrate . J. Dairy Res. , 44 : 509
  • de Wit , J. N. , Klarenbeek , G. and Hontelez‐Backx , E. 1983 . Evaluation of functional properties of whey protein concentrates and whey protein isolates. I. Isolation and characterization . Neth. Milk Dairy J. , 37 : 37
  • Bernal , V. and Jelen , P. 1984 . Effect of calcium binding on thermal denaturation of bovine α‐lactalbumin . J. Dairy Sci. , 67 : 2452
  • Chaplin , L. C. and Lyster , R. L. J. 1986 . Irreversible heat denaturation of bovine α‐lactalbumin . J. Dairy Res. , 53 : 249
  • Fox , P. F. 1989 . “ The milk protein system ” . In Developments in Dairy Chemistry‐4 , Edited by: Fox , P. F. 1 New York : Elsevier Applied Science .
  • Owusu , R. K. 1992 . The effect of calcium on bovine α‐lactalbumin conformational transitions by ultraviolet difference and fluorescence spectrophotometry . Food Chem. , 43 : 41
  • Hiraoka , Y. , Segawa , T. , Kuwajima , K. , Sugai , S. and Murai , N. 1980 . α‐Lactalbumin: a calcium metallo‐protein . Biochim. Biophys. Res. Commun. , 95 : 1098
  • Spector , A. A. 1975 . Fatty acid binding to plasma albumin . J. Lipid Res. , 16 : 165
  • Gumpen , S. , Hegg , P. O. and Martens , M. 1979 . Thermal stabilization of fatty acid‐serum albumin complexes studied by differential scanning calorimetry . Biochim. Biophys. Acta , 574 : 189
  • Klemaszewski , J. L. , Das , K. P. , Kang , Y. J. and Kinsella , J. E. 1990 . Effects controlled sulfitolysis of bovine serum albumin on droplet size and surface area of emulsions . J. Agric. Food Chem. , 38 : 647
  • Lin , V. J. C. and Koenig , J. L. 1976 . Raman studies of bovine serum albumin . Biopolymers , 15 : 203
  • Brunner , J. R. 1977 . “ Milk proteins ” . In Food Proteins , Edited by: Whitaker , J. R. and Tannenbaum , S. R. 175 Westport, CT : AVI Publ. .
  • Kitchen , B. J. 1974 . A comparison of the properties of membranes isolated from bovine skim milk and cream . Biochim. Biophys. Acta , 356 : 257
  • Euber , J. R. and Brunner , J. R. 1984 . Reexamination of fat globule clustering and creaming in cow milk . J. Dairy Sci. , 67 : 2821
  • Williams , T. V. , Versteeg , K. and Houlihan , A. V. 1991 . The effect of pasteurisation of cheese milk and whey on the major lipid components of whey protein concentrates . Aust. J. Dairy Technol. , 46 : 36
  • Joseph , M. S. B. and Mangino , M. E. 1988 . The effects of milk fat globule membrane protein on the foaming and gelation properties of β‐lactoglobulin solutions and whey protein concentrates . Aust. J. Dairy Technol. , 43 : 9
  • Keenan , T. W. , Dylewski , D. P. , Woodford , T. A. and Ford , R. H. 1983 . “ Origin of milk fat globules and the nature of the milk fat globule membrane ” . In Developments in Dairy Chemistry‐2 , Edited by: Fox , P. F. 83 New York : Elsevier Applied Science .
  • Russell , C. E. , Matthews , M. E. and Gray , I. K. 1980 . A comparison of methods for the extraction of the fat from soluble whey protein concentrate powders . N.Z. J. Dairy Sci. Technol. , 15 : 239
  • Dapper , C. H. , Valivullah , H. M. and Keenan , T. W. 1987 . Use of polar aprotic solvents to release membranes from milk lipid globules . 70 : 760
  • de Wit , J. N. , Klarenbeek , G. and Adamse , M. 1986 . Evaluation of functional properties of whey protein concentrates and whey protein isolates. II. Effects of processing history and composition . Neth. Milk Dairy J. , 40 : 41
  • Rinn , J.‐C. , Morr , C. V. , Seo , A. and Surak , J. G. 1990 . Evaluation of nine semi‐pilot scale whey pretreatment modifications for producing whey protein concentrate . J. Food Sci. , 55 : 510
  • Reiter , B. 1983 . The biological significance of lactoferrins . Int. J. Tissue React. , 1 : 87
  • Reiter , B. 1985 . “ The biological significance of the non‐immunoglobulin protective proteins in milk: lysozyme, lactoferrin, lactoperoxidase ” . In Developments in Dairy Chemistry‐3 , Edited by: Fox , P. F. 281 New York : Elsevier Applied Science .
  • Kawakami , H. , Shinmoto , H. , Dosako , S. and Sogo , Y. 1987 . One‐step isolation of lactoferrin using immobilized monoclonal antibodies . J. Dairy Sci. , 70 : 752
  • Al‐Mashikhi , S. A. and Nakai , S. 1987 . Isolation of bovine immunoglobulins and lactoferrin from whey proteins by gel filtration techniques . J. Dairy Sci. , 70 : 2486
  • Kanno , C. 1989 . Purification and separation of multiple forms of lactophorin from bovine milk whey and their immunological and electrophoretic properties . J. Dairy Sci. , 72 : 883
  • Yoshida , S. 1988 . Isolation of some minor milk proteins, distributed in acid whey from approximately 100,000 to 250,000 daltons of particle size . J. Dairy Sci. , 71 : 1
  • Brock , J. H. 1985 . “ The transferrins ” . In Topics in Molecular and Structural Biology: Metallo Protein, (Part 2, Metal proteins with non‐redox roles) , Edited by: Harrison , P. M. 183 London : MacMillan .
  • Yoshida , S. 1989 . Preparation of lactoferrin by hydropho‐bic interaction chromatography from milk acid whey . J. Dairy Sci. , 72 : 1446
  • Harwalkar , V. R. 1980 . Kinetics of thermal denaturation of β‐lactoglobulin at pH 2.5 . J. Dairy Sci. , 63 : 1043
  • de Wit , J. N. and Klarenbeek , G. 1984 . Effects of various heat treatments on structure and solubility of whey proteins . J. Dairy Sci. , 67 : 2701
  • Kilara , A. and Sharkasi , T. Y. 1986 . Effects of temperature on food proteins and its implications on functional properties . CRC Crit. Rev. Food Sci. Nutr. , 23 : 323
  • Pearce , R. J. 1989 . “ Thermal denaturation of whey proteins ” . In Heat‐Induced Changes in Milk , 17 Brussels, , Belgium : Int. Dairy Fed. .
  • Morr , C. V. 1990 . Effect of heating and elevated temperature storage on cheese whey . J. Food Sci. , 55 : 1177
  • Morr , C. V. 1987 . The effect of HTST pasteurization of milk, cheese whey and cheese whey UF retentate upon the composition, physicochemical and functional properties of whey protein concentrates . J. Food Sci. , 52 : 312
  • Elfagm , A. A. and Wheelock , J. V. 1977 . Effect of heat on α‐lactalbumin and β‐lactoglobulin in bovine milk . J. Dairy Res. , 44 : 367
  • Patocka , J. , Drathen , M. and Jelen , P. 1987 . Heat stability of isolated whey protein fractions in highly acidic conditions . Milchwissenschaft , 42 : 700
  • Hermansson , A.‐M. 1979 . “ Aggregation and denaturation involved in gel formation ” . In Functionality and Protein Structure , Edited by: Pour‐El , A. 81 Washington, D.C. : Am. Chem. Soc. .
  • Zittle , C. A. , DellaMonica , E. S. , Rudd , R. K. and Custer , J. H. 1957 . The binding of calcium ions by β‐Iactoglobulin both before and after aggregation by heating in the presence of calcium ions . J. Am. Chem. Soc. , 79 : 4661
  • Hilalgo , J. and Gamper , E. 1977 . Solubility and heat stability of whey protein concentrates . J. Dairy Sci. , 60 : 1515
  • Li‐Chan , E. 1983 . Heat‐induced changes in the proteins of whey protein concentrate . J. Food Sci. , 48 : 47
  • Li‐Chan , E. 1983 . Properties and molecular interactions of whey protein concentrates upon storage . J. Dairy Sci. , 66 : 1843
  • Morr , C. V. 1979 . Review: functionality of whey protein products . NZ. J. Dairy Sci. Technol. , 14 : 185
  • Shen , J. L. 1981 . “ Solubility and viscosity ” . In Protein Functionality in Foods , Edited by: Cherry , J. P. 89 Washington, D.C. : Am. Chem. Soc. .
  • Schmidt , R. H. 1981 . “ Gelation and coagulation ” . In Protein Functionality in Foods , Edited by: Cherry , J. P. 131 Washington, D.C. : Am. Chem. Soc. .
  • Ziegler , G. R. and Foegeding , E. A. 1990 . The gelation of proteins . Adv. Food Res. , 34 : 203
  • Barker , C. M. and Morr , C. V. 1986 . Composition and properties of Spherosil‐QMA whey protein concentrate . J. Food Sci. , 51 : 919
  • Quequiner , C. , Dumay , E. , Salou‐Cavalier , C. and Cheftel , J. D. 1992 . Microcoagulation of a whey protein isolate by extrusion cooking at acid pH . J. Food Sci. , 57 : 610
  • Leman , J. and Kinsella , J. E. 1989 . Surface activity, film formation, and emulsifying properties of milk proteins . CRC Crit. Rev. Food Sci. Nutr. , 28 : 115
  • Phillips , L. G. , Yang , S. T. and Kinsella , J. E. 1991 . Neutral salt effects on stability of whey protein isolate foams . J. Food Sci. , 56 : 588
  • Dickinson , E. and Stainsby , G. 1988 . “ Emulsion stability ” . In Advances in Food Emulsions and Foams , Edited by: Dickinson , E. and Stainsby , G. 1 New York : Elsevier Applied Science .
  • Mangino , M. E. 1989 . “ Molecular properties and functionality of proteins in food emulsions: liquid food systems ” . In Food Proteins , Edited by: Kinsella , J. E. and Soucie , W. C. 159 Champaign, IL : Am. Oil Chem. Soc. .
  • Tornberg , E. and Hermansson , A.‐M. 1977 . Functional characterization of protein‐stabilized emulsions: effect of processing . J. Food Sci. , 42 : 468
  • Pearce , K. N. and Kinsella , J. E. 1978 . Emulsifying properties of proteins: evaluation of a turbidimetric technique . J. Agric. Food Chem. , 26 : 716
  • Haque , Z. and Kinsella , J. E. 1988 . Emulsifying properties of food proteins: bovine serum albumin . J. Food Sci. , 53 : 416
  • Haque , Z. and Kinsella , J. E. 1989 . Emulsifying properties of food proteins: development of a standardized emulsification method . J. Food Sci. , 54 : 39
  • Lee , S.‐H. , Morr , C. V. and Ha , E. Y. W. 1992 . Structural and functional properties of caseinate and whey protein isolate as a function of temperature and pH . J. Food Sci. , 57 : 1210
  • Klemaszewski , J. L. , Das , K. P. and Kinsella , J. E. 1992 . Formation and coalescence stability of emulsions stabilized by different milk proteins . J. Food Sci. , 57 : 366
  • Ha , E. Y. W. , Morr , C. V. , Ali , N. and Hsieh , C.‐Y. 1992 . Dynamic headspace analysis of volatile constituents of Swiss cheese whey protein concentrate . Int. Dairy J. , unpublished
  • McGugan , W. A. , Larmond , E. and Emmons , D. B. 1979 . Some observations on the flavor of acid whey . J. Inst. Can. Sci. Technol. , 12 : 32
  • Hill , L. M. , Hammond , E. G. , Carlin , A. F. and Seals , R. G. 1969 . Organoleptic evaluation of the effectiveness of antioxidants in milk fat . J. Dairy Sci. , 52 : 1917
  • Ferretti , A. , Flanagan , V. P. and Ruth , J. M. 1970 . Nonenzymatic browning in a lactose‐casein model system . J. Agric. Food Chem. , 18 : 13
  • Ferretti , A. and Flanagan , V. P. 1971 . Volatile constituents of whey powder subjected to accelerated browning . J. Dairy Sci. , 54 : 1764
  • Ferretti , A. and Flanagan , V. P. 1971 . Non‐enzymatic browning in edible spray‐dried whey. Identification of some volatile components . J. Dairy Sci. , 54 : 1769
  • Ferretti , A. and Flanagan , V. P. 1972 . Steam volatile constituents of stale nonfat dry milk. The role of the Maillard reaction in staling . J. Agric. Food Chem. , 20 : 695
  • Gaylord , A. M. , Warthesen , J. J. and Smith , D. E. 1986 . Influence of milk fat, milk solids, and light intensity on the light stability of vitamin A and riboflavin in lowfat milk . J. Dairy Sci. , 69 : 2779
  • Hammond , E. G. 1989 . “ The flavor of dairy products ” . In Flavor Chemistry of Lipid Foods , Edited by: Min , D. B. and Smouse , T. H. 222 Champaign, IL : Am. Oil Chem. Soc. .
  • O'Brien , J. M. and Morrissey , P. A. 1989 . “ The Maillard reaction in milk products ” . In Heat Induced Changes in Milk , 7 Brussels, , Belgium : Int. Dairy Fed. .
  • Labuza , T. P. and Saltmarch , M. 1981 . Kinetics of browning and protein quality loss in whey powders during steady state and nonsteady state storage conditions . J. Food Sci. , 47 : 92
  • Labuza , T. P. and Saltmarch , M. 1981 . “ The nonenzymatic browning reaction as affected by water in foods ” . In Water Activity: Influences on Food Quality , Edited by: Rockland , L. and Stewart , G. E. 605 New York : Academic Press .
  • Benkler , K. F. and Reinesscius , G. A. 1980 . Flavor isolation from fatty foods via solvent extraction and membrane dialysis . J. Food Sci. , 45 : 1084
  • Badings , H. T. and de Jong , C. 1983 . “ Headspace analysis for the study of aroma compounds in drinking juices ” . In Analysis of Volatiles , Edited by: de Gruyter , W. 401 New York : Methods‐Applications .
  • Mills , O. E. 1986 . A headspace sampling method for monitoring flavor volatiles of protein products . N.Z. J. Dairy Sci. Technol. , 21 : 49
  • Vandeweghe , P. and Reineccius , G. A. 1990 . Comparison of flavor isolation techniques applied to Cheddar cheese . J. Agric. Food Chem. , 38 : 1549
  • Manning , D. J. and Moore , C. 1979 . Headspace analysis of hard cheeses . J. Dairy Res. , 46 : 539
  • Wellnitz‐Ruen , W. , Reineccius , G. A. and Thomas , E. L. 1982 . Analysis of the fruity off‐flavor in milk using headspace concentration capillary column gas chromatography . J. Agric. Food Chem. , 32 : 512
  • Calvo , M. M. and Hoz , L. 1992 . Flavours of heated milks: a review . Int. Dairy J. , 2 : 69
  • Pokorny , J. 1981 . Browning from lipid‐protein interactions . Prog. Food Nutr. Sci. , 5 : 421
  • Fors , S. 1983 . “ Sensory properties of volatile Maillard Reaction products and related compounds: a literature review ” . In The Maillard Reaction in Foods and Nutrition , Edited by: Comstock , M. J. 185 Washington, D.C. : Am. Chem. Soc .
  • Allen , J. C. and Parks , O. W. 1979 . Photodegradation of riboflavin in milks exposed to fluorescent light . J. Dairy Sci. , 62 : 1377
  • Deger , D. and Ashoor , H. 1987 . Light‐induced changes in taste, appearance, odor, and riboflavin content of cheese . J. Dairy Sci. , 70 : 1371
  • Toyosaki , T. and Mineshita , T. 1990 . Mechanism of riboflavin photolysis studies by its decomposition products in model systems . Milchwissenschaft , 45 : 80
  • de Boer , R. , de Wit , J. N. and Hiddink , J. 1977 . Processing of whey by means of membranes and some applications of whey protein concentrate (in soft drinks and in flour confectionery) . J. Soc. Dairy Technol. , 30 : 112
  • Nakai , S. 1983 . Structure‐function relationships of food proteins with an emphasis on the importance of protein hydrophobicity . J. Agric. Food Chem. , 31 : 676
  • Harper , W. J. 1984 . Model food system approaches for evaluating whey protein functionality . J. Dairy Sci. , 67 : 2745
  • Liao , S. Y. and Mangino , M. E. 1987 . Characterization of the composition, physicochemical and functional properties of acid whey protein concentrates . J. Food Sci. , 52 : 1033
  • Fligner , K. L. and Mangino , M. E. 1991 . “ Relationship of composition to protein functionality ” . In Interactions of Food Proteins , Edited by: Parris , N. and Barford , R. 1 Washington, D.C. : Am. Chem. Soc. .
  • Yoshida , S. 1988 . Isolation of Iactoperoxidase of 89,000 daltons and a globulin of 81,000 daltons from milk acid whey . J. Dairy Sci. , 71 : 2021
  • Schmidt , R. H. , Illingworth , B. L. , Deng , J. C. and Cornell , J. A. 1979 . Multiple regression and response surface analysis of the effects of calcium chloride and cysteine on heat‐induced whey protein gelation . J. Agric. Food Chem. , 27 : 529
  • Dunkerley , J. A. and Hayes , J. F. 1980 . Characterization of whey protein gels using a temperature gradient block . N.Z. J. Dairy Sci. Technol. , 15 : 191
  • Zirbel , F. and Kinsella , J. E. 1988 . Factors affecting the Theological properties of gels made from whey protein isolate . Milchwissenschaft , 43 : 691
  • Gault , P. , Mahaut , M. and Korolczuk , J. 1990 . Caractéristiques rhéologiques et gélification thermique du concentré de protéines de lactosérum . Lait , 70 : 217
  • Kuhn , P. R. and Foegeding , E. A. 1991 . Factors influencing whey protein gel rheology: dialysis and calcium chelation . J. Food Sci. , 56 : 789
  • Kohnhorst , A. L. and Mangino , M. E. 1985 . Prediction of the strength of whey protein gels based on composition . J. Food Sci. , 50 : 1403
  • Schmidt , R. H. , Illingworth , B. L. , Ahmed , E. M. and Richter , R. L. 1978 . The effect of dialysis on heat‐induced gelation of whey protein concentrate . J. Food Process. Preserv. , 2 : 111
  • Schmidt , R. H. and Morris , H. A. 1984 . Gelation properties of milk proteins, soy proteins, and blended protein systems. . Food Technol. , 38 : 85
  • Fauquant , J. , Vieco , E. , Brule , G. and Maubois , J. L. 1985 . Clarification des lactoserum doux par agrégation thermocalcique de la matière grasse résiduelle . Lait , 65 : 1
  • Kato , A. and Nakai , S. 1980 . Hydrophobicity determined by a fluorescence probe method and its correlation with surface properties of proteins . Biochim. Biophys. Acta. , 624 : 13
  • Mangino , M. E. , Kim , J. H. , Dunkerly , J. E. and Zadow , J. G. 1987 . Factors important to the gelation of whey protein concentrates . Food Hydrocolloids , 1 : 227
  • Joseph , M. S. B. and Mangino , M. E. 1988 . Contribution of milk fat globule membrane proteins to the effective hydrophobicity of whey protein concentrates . Aust. J. Dairy Technol. , 43 : 6
  • Schmidt , R. H. and Illingworth , B. L. 1978 . Gelation properties of whey protein and blended protein systems . Food Prod. Dew. , 12 : 60
  • Sternberg , M. , Chiang , J. P. and Ebert , N. J. 1976 . Cheese whey proteins isolated with polyacrylic acid . J. Dairy Sci. , 59 : 1042
  • Garrett , J. M. , Stairs , R. A. and Annett , R. G. 1988 . Thermal denaturation and coagulation of whey proteins: effects of sugars . J. Dairy Sci. , 71 : 10
  • Gossett , P. W. , Rizvi , S. S. H. and Baker , R. C. 1984 . Quantitative analysis of gelation in egg protein systems . Food Technol. , 38 : 67
  • Shimada , K. and Cheftel , J. C. 1989 . Sulfhydryl group/ disulfide bond interchange reactions during heat‐induced gelation of whey protein isolate . J. Agric. Food Chem. , 37 : 161
  • Langley , K. R. and Green , M. L. 1989 . Compression and impact strength of gels prepared from fractionated whey proteins, in relation to composition and micro‐structure . J. Dairy Res. , 56 : 275
  • Hillier , R. M. and Cheeseman , G. C. 1979 . Effect of proteose‐peptone on the heat‐gelation of whey protein isolates. . Dairy Res. , 46 : 113
  • Flory , P. J. 1974 . Introductory lecture . Faraday Discuss. Chem. Soc. , 57 : 7
  • Hegg , P. O. and Lofqvist , B. 1974 . The protective effect of small amounts of anionic detergents on the thermal aggregation of crude ovalbumin . J. Food Sci. , 39 : 1231
  • McKenzie , H. A. , Ralston , G. B. and Shaw , D. C. 1971 . Location of sulfhydryl and disulfide groups in bovine β‐lactoglobulins and effects of urea . Biochemistry , 11 : 4539
  • Delaney , R. A. M. 1976 . Composition, properties and uses of whey protein concentrates . J. Soc. Dairy Technol. , 29 : 91
  • Bernal , V. M. , Smajda , D. H. , Smith , J. L. and Stanley , D. W. 1987 . Interactions in protein/polysaccha‐ride/calcium gels . J. Food Sci. , 52 : 1121
  • Damodaran , S. and Kinsella , J. E. 1981 . The effects of neutral salts on the stability of macromolecules . J. Biol. Chem. , 256 : 3394
  • Babajimopoulos , M. , Damodaran , S. , Rizvi , S. S. H. and Kinsella , J. E. 1983 . Effects of various anions on the rheological and gelling behavior of soy proteins: thermodynamics observations . J. Agric. Food Chem. , 3 : 1270
  • Peltonen‐Shalaby , R. and Mangino , M. E. 1986 . Compositional factors that affect the emulsifying and foaming properties of whey protein concentrates . J. Food Sci. , 51 : 91
  • de Wit , J. N. and de Boer , R. 1975 . Ultrafiltration of cheese whey and some functional properties of the resulting whey protein concentrate . Neth. Milk Dairy J. , 29 : 198
  • Richert , S. H. , Morr , C. V. and Cooney , C. M. 1974 . Effects of heat and other factors upon foaming properties of whey protein concentrates . J. Food Sci. , 39 : 42
  • Wong , N. P. , LaCoix , D. E. and McDonough , F. E. 1978 . Minerals in whey and whey fractions . J. Dairy Sci. , 61 : 1700
  • Kim , Y. A. , Chism , G. W. III and Mangino , M. E. 1987 . Determination of the beta‐lactoglobulin, alpha‐lactal‐bumin and bovine serum albumin of whey protein concentrates and their relationship to protein functionality . J. Food Sci. , 52 : 124
  • Li‐Chan , E. and Nakai , S. 1989 . “ Effect of molecular changes (SH groups and hydrophobicity) of food proteins on their functionality ” . In Food Proteins , Edited by: Kinsella , J. E. and Soucie , W. G. 232 Champaign, IL : Am. Oil Chem. Soc. .
  • Nakai , S. and Li‐Chan , E. 1989 . “ Effects of heating on protein functionality ” . In Protein Quality and the Effects of Processing , Edited by: Phillips , R. D. and Finley , J. W. 125 New York : Marcel Dekker .
  • Mangino , M. E. . Functionality of whey protein concentrates . Proc. Dairy Prod. Tech. Conf. . pp. 73 Chicago : Am. Dairy Prod. Inst. .
  • Nakai , S. and Li‐Chan , E. 1988 . Hydrophobic Interactions in Food Systems , Boca Raton, FL : CRC Press .
  • Mangino , M. E. , Huffman , L. M. and Regester , G. O. 1988 . Changes in the hydrophobicity and functionality of whey during the processing of whey protein concentrates . J. Food Sci. , 53 : 1684
  • Mangino , M. E. , Fritsch , D. A. and Liao , S. Y. 1985 . The binding of N—alkanes to whey protein concentrates as a predictor of their functionality . N.Z. J. Dairy Sci. Technol. , 20 : 103
  • Shimizu , M. , Saito , M. and Yamauchi , K. 1986 . Hydrophobicity and emulsifying activity of milk proteins . Agric. Biol. Chem. , 50 : 791
  • Sklar , L. A. , Hudson , B. S. and Simoni , R. D. 1977 . Conjugated polyene fatty acids as probes: synthetic phospholipid membrane studies . Biochemistry , 16 : 819
  • Mohammadzadeh‐K , A. , Feeney , R. E. and Smith , L. M. 1969 . Hydrophobic binding of hydrocarbons by proteins. I. Relationship of hydrocarbon structure . Biochim. Biophys. Acta , 194 : 246
  • Ingraham , R.H. , Lau , S.Y.M. , Taneja , A.K and Hodges , R. S. 1985 . Denaturation and effect of temperature on hydrophobic‐interaction and reversed‐phase high performance liquid chromatography of proteins: Bio‐gel TSK‐phenyI‐5‐PW column . J. Chromatogr. , 327 : 77
  • Voutsinas , L. P. and Nakai , S. 1983 . A simple turbidimet‐ric method for determining the fat binding capacity of proteins . J. Agric. Food Chem. , 31 : 58
  • Tsutsui , T. , Li‐Chan , E. and Nakai , S. 1986 . A simple fluorometric method for fat binding capacity as an index of hydrophobicity of proteins . J. Food Sci. , 51 : 1268
  • Kato , A. , Matsuda , T. , Matsudomi , N. and Kobayashi , K. 1984 . Determination of protein hydrophobicity using a sodium dodecyl sulfate binding method . J. Agric. Food Chem. , 32 : 284
  • Hayakawa , S. and Nakai , S. 1985 . Relationships of hydrophobicity and net charge to the solubility of milk and soy proteins . J. Food Sci. , 50 : 486
  • Mulvihill , D. M. and Fox , P. F. 1989 . “ Physico‐chemical and functional properties of milk proteins ” . In Developments in Dairy Chemistry , Edited by: Fox , P. F. Vol. 4 , 131 New York : Elsevier Applied Science .
  • Shimizu , M. , Saito , M. and Yamauchi , K. 1985 . Emulsifying and structural properties of β‐lactoglobu‐lin at different pHs . Agric. Biol. Chem. , 49 : 189
  • Harris , J.L. , Pecar , M. A. and Pearce , R. J. 1989 . Effect of the processing equipment on protein functionality in the concentration of cheese whey by ultrafiltration . Aust. J. Dairy Technol. , 44 : 78
  • Nakai , S. , Ho , L. , Helbig , N. , Katp , A. and Tung , M. A. 1980 . Relationship between hydrophobicity and emulsifying properties of some plant proteins . Can. Inst. Food Sci. Technol. , 13 : 23
  • Kato , A. , Komatsu , K. , Fujimoto , K. and Kobayashi , K. 1985 . Relationship between surface functional properties and flexibility of proteins detected by the protease susceptibility . J. Agric. Food Chem. , 33 : 931
  • Kato , A. , Nagase , Y. , Matsudomi , N. and Kobayashi , K. 1983 . Determination of molecular weight of soluble ovalbumin aggregates during heat denatur‐ation using low cycle laser light scattering technique . Agric. Biol. Chem. , 47 : 1829
  • Voutsinas , L. P. , Cheung , E. and Nakai , S. 1983 . Relationships of hydrophobicity to emulsifying properties of heat denatured proteins . J. Food Sci. , 48 : 26
  • Townsend , A. A. and Nakai , S. 1983 . Relationships between hydrophobicity and foaming characteristics of food proteins . J. Food Sci. , 48 : 588
  • Kinsella , J. E. and Whitehead , D. M. 1988 . “ Properties of chemically modified proteins ” . In Advances in Food Emulsions and Foams , 163 New York : Elsevier Applied Science .
  • Kato , A. , Fujimoto , K. , Matsudomi , N. and Kobayashi , K. 1986 . Protein flexibility and functional properties of heat‐denatured ovalbumin and lysozyme . Anal. Biol. Chem. , 50 : 417
  • Cooney , C. M. and Morr , C. V. 1972 . Hydrogen peroxide alteration of whey proteins in whey and concentrated whey systems . J. Dairy Sci. , 55 : 567
  • Morr , C. V. 1985 . Functionality of heated milk proteins in dairy and related foods . J. Dairy Sci. , 68 : 2773
  • Mangino , M. E. , Liao , Y. Y. , Harper , W. J. , Morr , C. V. and Zadow , J. G. 1987 . The effects of heat processing on the functionality of whey protein concentrates . J. Food Sci. , 52 : 1522
  • Modler , H. W. and Emmons , D. B. 1977 . Properties of whey protein concentrate prepared by heating under acidic conditions . J. Daily Sci. , 60 : 177
  • McDonough , F. E. , Hargrove , R. E. , Nattingly , W. A. , Postati , C. P. and Alford , J. A. 1974 . Composition and properties of whey protein concentrates from ultrafiltration . J. Dairy Sci. , 57 : 1438
  • Maubois , J. L. 1991 . New applications of membrane technology in the dairy industry . Aust. J. Dairy Technol. , 46 : 91
  • Kim , S. H. , Morr , C. V. , Seo , A. and Surak , J. G. 1989 . Effect of whey pretreatment on composition and func‐ tional properties of whey protein concentrate . J. Food Sci. , 54 : 25
  • Grindstaff , D. Process for Pretreating Raw Whey . U.S. Patent 3,864,506 . 1975 .
  • Lehmann , H. and Wasen , D. Method of De‐phospholidating Whey . U.S. Patent 4,897,279 . 1990 .
  • Lee , C. N. and Merson , R. L. 1976 . Prefiltration of cottage cheese whey to reduce fouling of ultrafiltration membrane . J. Food Sci. , 41 : 402
  • Ennis , B. M. , Johns , J. E. M. and O'Connell , M. T. 1981 . The effect of the replacement of calcium with sodium on the ultrafiltration of acid whey . N.Z. J. Dairy Sci. Technol. , 16 : 69
  • Merin , N. , Gordin , S. and Tanny , G. B. 1983 . Microfiltration of sweet cheese whey . N.Z. J. Dairy Sci. Technol. , 18 : 153
  • Olsen , N. and Jensen , F. 1989 . Microfiltration: the influence of operation parameters on the process . Milchwissenschaft , 44 : 476
  • Knopp , T. K. 1992 . “ Lipoprotein Removal from Cheese Whey by Cross‐flow Microfiltration ” . Ohio State University . M.Sci. thesis
  • Spencer , G. and Thomas , R. L. 1991 . Fouling, cleaning, and rejuvenation of formed‐in‐place membranes . Food Technol. , 45 : 98
  • Bouchet , P. and Houdoux , A. 1989 . Effect of pretreatment and drying condition on compositional and functional properties of whey protein concentrate . unpublished
  • Tratnik , L. and Krsev , L. 1991 . Production of whey protein concentrates . Milchwissenschaft , 46 : 91
  • Duxbury , D. D. 1992 . Advanced process for whey protein concentrate increases yield . Food Process. , 53 : 82
  • Nichols , J. A. and Morr , C. V. 1985 . Spherosil‐S ion exchange process for preparing whey protein concentrate . J. Food Sci. , 50 : 610
  • Pearce , R. J. 1983 . Thermal separation of β‐lactoglobulin and α‐lactalbumin in bovine Cheddar cheese whey . Aust. J. Daily Technol. , 38 : 144
  • Ker , Y. C. and Toledo , R. T. 1992 . Influence of shear treatments on consistency and gelling properties of whey protein isolate suspensions . J. Food Sci. , 57 : 82
  • Avers , J. S. and Peterson , M. J. 1985 . Whey protein recovery using a range of novel ion‐exchangers . N.Z. J. Dairy Sci. Technol. , 20 : 129
  • Avers , J. S. , Elgar , D. F. and Petersen , M. J. 1986 . Whey protein recovery using Indion S, an industrial ion exchanger for proteins . N.Z. J. Dairy Sci. Technol. , 21 : 21
  • Lopez‐Leiva , M. H. 1988 . The use of electrodialysis in food processing. I. Some theoretical concepts . Lebensm. Wiss. Technol. , 21 : 119
  • Lopez‐Leiva , M. H. 1988 . The use of electrodialysis in food processing. II. Review of practical applications . Lebensm. Wiss. Technol. , 21 : 177
  • Chen , J. P. and Wang , C. H. 1991 . Microfiltration affinity purification of lactoferrin and immunoglobulin G from cheese whey . J. Food Sci. , 56 : 701
  • Rechtor , J. , Matsudomi , N. and Kinsella , J. E. 1991 . Changes in gelling behavior of whey protein isolate and β‐lactoglobulin during storage: possible mechanism(s) . J. Food Sci. , 56 : 782
  • Hsu , K. H. and Fennema , O. 1989 . Changes in the functionality of dry whey protein concentrate during storage . J. Dairy Sci. , 72 : 829

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