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DNA Sequence Volume 15, 2004 - Issue 3
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Research Article

Cloning and Sequencing of an Epoxide Hydrolase Gene from Rhodosporidium paludigenum

Michel Labuschagne Department of Microbial, Biochemical and Food Biotechnology University of the Free State P.O. Box 339 9300 Bloemfontein South Africa
,
Adriana L. Botes Department of Microbial, Biochemical and Food Biotechnology University of the Free State P.O. Box 339 9300 Bloemfontein South Africa
&
Jacobus Albertyn Department of Microbial, Biochemical and Food Biotechnology University of the Free State P.O. Box 339 9300 Bloemfontein South Africa
Pages 202-205 | Published online: 11 Jul 2009

References

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  • Arand, M., Mu¨ ller, F., Mecky, A., Hinz, W., Urban, P., Pompon, D., Kellner, R. and Oesch, F. (1999) “Catalytic triad of microsomal epoxide hydrolase: replacement of Glu404 with Asp leads to a strongly increased turnover rate”, Biochemical Journal 337, 37–43.
  • Archelas, A. and Furstoss, R. (2001) “Synthetic applications of epoxide hydrolases”, Current Opinion in Chemical Biology 5, 112– 119.
  • Lacourciere, G.M. and Armstrong, R.N. (1993) “The catalytic mechanism of microsomal epoxide hydrolase involves an ester intermediate”, Journal of the American Chemical Society 115, 10466– 10467.
  • Ochman, H., Gerber, A.S. and Hartl, D.L. (1988) “Genetic application of an inverse polymerase chain reaction”, Genetics 120, 621– 623.
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  • Orru, V.A.R. and Faber, K. (1999) “Stereoselectivities of microbial epoxide hydrolases”, Current Opinion in Chemical Biology 3, 16– 21.
  • Visser, H., De Bont, J.A.M. and Verdoes, J.C. (1999) “Isolation and characterization of the epoxide hydrolase encoding gene from Xanthophyllomyces dendrorhous”, Applied and Environmental Microbiology 65, 5459– 5463.
  • Visser, H., Vreugdenhil, S., De Bont, J.A.M. and Verdoes, J.C. (2000) “Cloning and characterization of an epoxide hydrolase encoding gene from Rhodotorula glutinis”, Applied Microbial Biotechnology 53, 415– 419.
  • Visser, H., Weijers, C.A.M.G., Van Ooyen, A.J.J. and Verdoes, J.C. (2002) “Cloning, characterization and heterologous expression of epoxide hydrolase-encoding cDNA sequences from yeast belonging to the genera Rhodotorula and Rhodosporidium”, Biotechnology Letters 24, 1687– 1694.

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