Evaluation of real-time PCR as an alternative for potency testing of Brucella abortus vaccines

References

• Kollannur JD, Rathore R, Chauhan RS. Epidemiology and economics of brucellosis in animals and its zoonotic significance. XII International Society of Animal Hygiene (ISAH). 2007;466–468.
   Google Scholar
• Singh BB, Khatkar MS, Aulakh RS, Gill JPS, Dhand NK. Estimation of the health and economic burden of human brucellosis in India. Prevent Vet Med. 2018;154:148–155.
   PubMed Web of Science ®Google Scholar
• Singh BB, Kostoulas P, Gill JP, Dhand NK. Cost-benefit analysis of intervention policies for prevention and control of brucellosis in India. PLoS Negl Trop Dis. 2018;12(5):e0006488.
   PubMed Web of Science ®Google Scholar
• PD-ADAMS. National Control Programme on Brucellosis: Aim and Strategies. Bengaluru, India; 2012.
   Google Scholar
• Schurig GG, Sriranganathan N, Corbel MJ. Brucellosis vaccines: past, present and future. Vet Microbiol. 2002;90(1–4):479–496.
   PubMed Web of Science ®Google Scholar
• Moriyón I, Grilló MJ, Monreal D, et al. Rough vaccines in animal brucellosis: structural and genetic basis and present status. Vet Res. 2004;35(1):1–38.
   PubMed Web of Science ®Google Scholar
• OIE. Bovine Brucellosis. OIE Terrestrial Manual. Paris, France: OIE-the World Organization for Animal Health; 2016:1–35.
   Google Scholar
• Indian pharmacopoeia. Vol. III: section 2.2.11. Government of India, Ministry of Health and Family Welfare, Indian Pharmacopoeia Commission, Ghaziabad. 2014.
   Google Scholar
• Forsey T, Heath AB, Minor PD. A collaborative study to assess the proficiency of laboratory estimates of potency of live measles vaccines. Biologicals. 1992;20(3):233–242.
   PubMed Web of Science ®Google Scholar
• Forsey T, Heath AB, Minor PD. A European collaborative study to assess the proficiency of laboratory estimates of potency of live measles, mumps and rubella tri-valent vaccines. Biologicals. 1993;21(3):239–249.
   PubMed Web of Science ®Google Scholar
• Fukuda A, Şengün F, Sarpay HE, et al. Parameters for plaque formation in the potency assay of Japanese measles vaccines. J Virol Meth. 1996;61(1–2):1–6.
   PubMed Web of Science ®Google Scholar
• Baron EJ, Miller JM, Weinstein MP, et al. A guide to utilization of the microbiology laboratory for diagnosis of infectious diseases: 2013 recommendations by the Infectious Diseases Society of America (IDSA) and the American Society for Microbiology (ASM). Clin Infect Dis. 2013;57(4):e22–e121.
   PubMed Web of Science ®Google Scholar
• Bricker BJ, Halling SM. Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51. Journal of Clinical Microbiology. 1995;33(6):1640–1642.
   Google Scholar
• García-Yoldi D, Marín CM, de Miguel MJ, Muñoz PM, Vizmanos JL, López-Goñi I. Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1. Clinical Chemistry. 2006;52(4):779–781.
   Google Scholar
• Kang SI, Her M, Kim JW, Kim JY, Ko KY, Ha YM, Jung SC. Advanced multiplex PCR assay for differentiation of Brucella species. Applied and Environmental Microbiology. 2011;77(18):6726–6728
   Google Scholar
• Sangari FJ, García-Lobo JM, Agüero J. The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes. FEMS Microbiol Lett. 1994;121(3):337–342.
   PubMed Web of Science ®Google Scholar
• Alton GG, Jones LM, Pietz DE. Laboratory techniques in brucellosis. Monogr Ser World Health Organ. 1975;55:1–163.
   Google Scholar
• Das A, Kumar B, Chakravarti S, et al. Rapid visual isothermal nucleic acid‐based detection assay of Brucella species by polymerase spiral reaction. J. Appl. Microbiol. 2018;125(3):646–654.
   PubMed Web of Science ®Google Scholar
• Altschul SF, Gish W. Local alignment statistics. Methods Enzymol. 1996;266:460–480.
   PubMed Web of Science ®Google Scholar
• Prediger E. https://eu.idtdna.com/pages/education/decoded/article/calculations-converting-from-nanograms-to-copy-number 2013.
   Google Scholar
• Schalk JA, van den Elzen C, Ovelgönne H, Baas C, Jongen PM. Estimation of the number of infectious measles viruses in live virus vaccines using quantitative real-time PCR. J Virol Meth. 2004;117(2):179–187.
   PubMed Web of Science ®Google Scholar
• Kallesh DJ, Hosamani M, Balamurugan V, Bhanuprakash V, Yadav V, Singh RK. Quantitative PCR: a quality control assay for estimation of viable virus content in live attenuated goat pox vaccine. Ind J Exp Biol. 2009;47(11):911–915.
   PubMed Web of Science ®Google Scholar
• Ranheim T, Mathis PK, Joelsson DB, et al. Development and application of a quantitative RT-PCR potency assay for a pentavalent rotavirus vaccine (RotaTeq®). J Virol Meth. 2006;131(2):193–201.
   PubMed Web of Science ®Google Scholar
• Sastry L, Johnson T, Hobson MJ, Smucker B, Cornetta K. Titering lentiviral vectors: comparison of DNA, RNA and marker expression methods. Gene Ther. 2002;9(17):1155–1162.
   PubMed Web of Science ®Google Scholar
• Prabhu M, Sankar MS, Bhanuprakash V, et al. Real time PCR: a rapid tool for potency estimation of live attenuated camelpox and buffalopox vaccines. Biologicals. 2012;40(1):92–95.
   PubMed Web of Science ®Google Scholar
• Wang F, Puddy AC, Mathis BC, et al. Using QPCR to assign infectious potencies to adenovirus based vaccines and vectors for gene therapy: toward a universal method for the facile quantitation of virus and vector potency. Vaccine. 2005;23(36):4500–4508.
   PubMed Web of Science ®Google Scholar
• Halling SM, Tatum FM, Bricker BJ. Sequence and characterization of an insertion sequence, IS711, from Brucella ovis. Gene. 1993;133(1):123–127.
   PubMed Web of Science ®Google Scholar
• Ouahrani S, Michaux S, Widada JS, et al. Identification and sequence analysis of IS6501, an insertion sequence in Brucella spp.: relationship between genomic structure and the number of IS6501 copies. Microbiology. 1993;139(12):3265–3273.
   Google Scholar
• Cloeckaert A, Grayon M, Grepinet O. An IS711 element downstream of thebp26 gene is a specific marker of Brucella spp. isolated from marine mammals. Clin. Diagn. Lab. Immunol. 2000;7:835–839.
   PubMedGoogle Scholar
• Patil MR, Kumar A, Bannalikar S, Dighe VD. eryC-5’nuclease PCR: differentiating wild Brucella strains from vaccine strain S19. TAJAS. 2014;9(2):207–211.
   Google Scholar
• Grilló MJ, Blasco JM, Gorvel JP, Moriyón I, Moreno E. What have we learned from brucellosis in the mouse model? Vet Res. 2012;43(1):29.
   PubMedGoogle Scholar