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Toxicology Methods Volume 9, 1999 - Issue 2
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Research Article

A SENSITIVE, HIGH-CAPACITY RADIOIMMUNOASSAY FOR METALLOTHIONEIN AND ITS APPLICATION TO URINE SAMPLES FROM CADMIUM-TREATED RATS

Zahir Shaikh Department of Biomedical Sciences, College of Pharmacy, University of Rhode Island, Kings ton, Rhode Island, USA
,
Jordan Scott Department of Biomedical Sciences, College of Pharmacy, University of Rhode Island, Kings ton, Rhode Island, USA
,
Kido Teruhiko Department of Biomedical Sciences, College of Pharmacy, University of Rhode Island, Kings ton, Rhode Island, USA
,
Tang Weifeng Department of Biomedical Sciences, College of Pharmacy, University of Rhode Island, Kings ton, Rhode Island, USA
,
Gross Walter Department of Fisheries, Animal and Veterinary Science, College of Life and Environmental Scien ces, University of Rhode Island, Kingston , Rhode Island, USA
&
Sabbioni Enrico Environment Institute, Joint Research Centre, European Commission, Ispra , Varese Italy
Pages 47-56 | Published online: 30 Sep 2008

  • Webb M. Metallothionein. In Webb M, ed. The Chemistry, Biochemistry and Biology of Cadmium. Amsterdam: EIsevier/North-Holland Biomedical;1979;195-266.
  • Hamer DH. Metallothionein. An nu Rev Biochem. 1986;55:913-951.
  • Eaton DL, Cherian MG. Determination of metalloth ionein in tissues by cadmium-hemoglobin affinity assay. Methods Enzymol. 1991;205:83-88.
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  • Summer KH, Klein D. Determination of metal loth ionein in biological materials. Methods Enzymol. 1991;205:57-60.
  • Tohyama C, Shaikh ZA. Cross-reactivity of metallothioneins from different origins with rabbit anti-rat hepatic metallothione in antibody. Biochem Biophys Res Commun. 1978;84:907-913.
  • Tohyama C, Shaikh ZA. Metallothionein in plasma and urine of cadmium-exposed rats determined bya single-antibody radioimmunoassay. Fundam AppIToxicol. 1981;1:1-7.
  • Nolan CV, Shaikh ZA. Determination of metallothionein in tissues by radioimmunoassay and by cadmium saturation method. Anal Biochem. 1986;154:213-223.
  • Shaikh ZA, Nolan CV Comparison of cadmium-saturation assay and radioimmunoassay for the determination of metallothionein concentration in tissues. In Kagi JHR, Kojima Y, eds. Metallothionein II. Basel: Birkhauser;1987;343-349.
  • Shaikh ZA. Radioimmunoassay for metallothionein in body fluids and tissues. Methods Enzymol. 1991;205:120-130.
  • Vander Mallie RJ, Garvey J S. Radioimmunoassay of metalloth ioneins. J Biol Chem. 1979;254:8416-8421.
  • Mehra RK, Bremner I. Development of a radioimmunoassay for rat liver metalloth ionein-I and its application to the analysis of rat plasma and kidneys. Biochem J. 1983;213:459-465.
  • Leibbrandt MEI, Koropatnick J, Harris J F, Cherian MG. Radioimmunoassay of metallothionein in rabbit, rat, mouse, Chinese hamster, and human ceIls. Biol Trace Elem Res. 1991;30:245-256.
  • Tohyama C, Shaikh ZA, Ellis KJ, Cohn SH. Metallothionein excretion in urine upon cadmium exposure: its relationship with liver and kidney cadmium. Toxicology. 1981;22:181-191.
  • Tohyama C, Shaikh ZA, Nogawa K, Kobayashi E, Honda R. Urinary metallothionein as a new index of renal dysfunction in "Itai-Itai"disease patients and other Japanese women environmentally exposed to cadmium. Arch Toxicol. 1982;50:159-166.
  • Shaikh ZA, Lucis OJ. Isolation of cadmium-binding proteins. Experientia. 1971;27:1024-1025.
  • Tang W, Kido T, Gross WA, Nogawa K, Sabbioni E, Shaikh ZA. Measurement of cadmiuminduced metallothionein in urine by ELISA and prevention of overestimation due to polymerization. J Anal Toxicol. 1999, vol. 23.
  • Heinegard D, Tiderstrom G. Determination of serum creatinine by a direct colorimetricmethod. Clin Chim Acta. 1973;43:305-310.
  • Shaikh ZA, Tohyama C. Urinary metallothionein as an indicator of cadmium body burden and of cadmium-induced nephrotoxicity. Environ Health Perspect. 1984;54:171-174.
  • Shaikh ZA, Harnett KM, Perlin SA, Huang PC. Chroniccadmium intake results in dose-related excretion of metallothionein in urine. Experientia. 1989;45:146-148.

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