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Biotechnic & Histochemistry Volume 79, 2004 - Issue 2
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Original Paper

Histochemical assay to detect class III ADH activity in situ in Arabidopsis seedlings

M Díaz Biochemistry and Molecular Biology Department, Building C, Autonomous University of Barcelona, 08193, Bellaterra (Barcelona), SpainCorrespondence[email protected]
,
MR Fernández Biochemistry and Molecular Biology Department, Building C, Autonomous University of Barcelona, 08193, Bellaterra (Barcelona), Spain
&
MC Martínez Biochemistry and Molecular Biology Department, Building C, Autonomous University of Barcelona, 08193, Bellaterra (Barcelona), Spain
Pages 91-94 | Received 06 Jan 2004, Accepted 07 Apr 2004, Published online: 12 Jul 2009

References

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  • Casanova-Schmitz M, David RM, Heck HD’A (1984) Oxidation of formaldehyde and acetaldehyde by NAD+- dependent dehydrogenases in rat nasal mucosal homo- genates. Biochem. Pharmacol. 33: 1137 – 1142. Connolly JH, Berlyn GP (1996) Cytochemical assay for differential respiratory activity in roots and root hairs. Biotech. & Histochem. 71: 197 – 201.
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  • Keller DA, Heck HD’A, Randall HW, Morgan KT (1990) Histochemical localization of formaldehyde dehydrogen- ase in the rat. Tox. & Appl. Pharmacol. 106: 311 – 326.
  • Koivusalo M, Baumann M, Uotila L (1989) Evidence for the identity of glutathione-dependent formaldehyde dehydrogenase and class III alcohol dehydrogenase. FEBS Lett. 257: 105 - 109.
  • Martı´nez SE, Vaglenova J, Sabria` J, Martı´nez MC, Farre´s J, Pare´s X (2001) Distribution of alcohol dehydro- genase mRNA in the rat central nervous system. Con- sequences for brain ethanol and retinoid metabolism. Eur. J. Biochem. 268: 5045– 5056.
  • Uotila L, Mannervik B (1979) A steady-state kinetic model for formaldehyde dehydrogenase from human liver. Biochem. J. 177: 869 - 878.
  • Uotila L, Koivusalo M (1987) Multiple forms of formal-dehyde dehydrogenase from human red blood cells. Hum. Hered. 37: 102 – 106.

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