A simple method for fixation and microdissection of frozen fresh tissue sections for molecular cytogenetic analysis of cancers

References

• Ahram M, Flaig MJ, Gillespie JW, Duray PH, Linehan WM, Ornstein DK, Niu S, Zhao Y, Petricoin EF, Emmert-Buck MR. Evaluation of ethanol fixed, paraffin embedded tissues for proteomic applications. Proteomics 2003; 3: 413–421
   PubMed Web of Science ®Google Scholar
• Ben-Ezra J, Johnson DA, Rossi J, Cook N, Wu A. Effect of fixation on the amplification of nucleic acids from paraffin embedded material by the polymerase chain reaction. J. Histochem. Cytochem. 1991; 39: 351–354
   PubMed Web of Science ®Google Scholar
• Craven RA, Banks RE. Laser capture microdissection and proteomics: possibilities and limitations. Proteomics 2001; 1: 1200–1204
   PubMed Web of Science ®Google Scholar
• Eltoum IA, Siegal GP, Frost AR. Microdissection of histologic sections: past, present, and future. Adv. Anat. Pathol. 2002; 9: 316–322
   PubMed Web of Science ®Google Scholar
• Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, Goldstein SR, Weiss RA, Liotta LA. Laser capture microdissection. Science 1996; 274: 998–1001
   PubMed Web of Science ®Google Scholar
• Giercksky HE, Thorstensen L, Qvist H, Nesland JM, Lothe RA. Comparison of genetic changes in frozen biopsies and microdissected archival material from the same colorectal liver metastases. Diagn. Mol. Pathol. 1997; 6: 318–325
   Google Scholar
• Hirose Y, Aldape K, Takahashi M, Berger MS, Feuerstein BG. Tissue microdissection and degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) is an effective method to analyze genetic aberrations in invasive tumors. J. Mol. Diagn. 2001; 3: 62–67
   PubMed Web of Science ®Google Scholar
• Huang Q, Schantz SP, Rao PH, Mo J, McCormick SA, Chaganti RS. Improving degenerate oligonucleotide primed PCR-comparative genomic hybridization for analysis of DNA copy number changes in tumors. Genes Chromosomes Cancer 2000; 28: 395–403
   Google Scholar
• Huang Q, Yu GP, McCormick SA, Mo J, Datta B, Mahimkar M, Lazarus P, Schaffer AA, Desper R, Schantz SP. Genetic differences detected by comparative genomic hybridization in head and neck squamous cell carcinomas from different tumor sites: construction of oncogenetic trees for tumor progression. Genes Chromosomes Cancer 2002; 34: 224–233
   PubMedGoogle Scholar
• Kallioniemi A, Kallioniemi OP, Sudar D, Rutovitz D, Gray JW, Waldman F, Pinkel D. Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science 1992; 258: 818–821
   PubMed Web of Science ®Google Scholar
• Kallioniemi OP, Kallioniemi A, Piper J, Isola J, Waldman FM, Gray JW, Pinkel D. Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumors. Genes Chromosomes Cancer 1994; 10: 231–243
   PubMed Web of Science ®Google Scholar
• Lee JY, Dong SM, Kim SY, Yoo NJ, Lee SH, Park WS. A simple, precise and economical microdissection technique for analysis of genomic DNA from archival tissue sections. Virchows Arch. 1998; 433: 305–309
   PubMed Web of Science ®Google Scholar
• Maitra A, Gazdar AF. Tissue microdissection and processing. Cancer Treat Res. 2001; 106: 63–84
   Google Scholar
• Moskaluk CA, Kern SE. Microdissection and polymerase chain reaction amplification of genomic DNA from histological tissue sections. Am. J. Pathol. 1997; 150: 1547–1552
   PubMed Web of Science ®Google Scholar
• Shen KL, Yang LS, Hsieh HF, Chen CJ, Yu JC, Tsai NM, Harn HJ. Microsatellite alterations on human chromosome 11 in in situ and invasive breast cancer: a microdissection microsatellite analysis and correlation with p53, ER (estrogen receptor), and PR (progesterone receptor) protein immunoreactivity. J. Surg. Oncol. 2000; 74: 100–107
   Google Scholar
• Speicher MR, du Manoir S, Schrock E, Holtgreve-Grez H, Schoell B, Lengauer C, Cremer T, Ried T. Molecular cytogenetic analysis of formalin fixed, paraffin embedded solid tumors by comparative genomic hybridization after universal DNA-amplification. Hum. Mol. Genet. 1993; 2: 1907–1914
   PubMedGoogle Scholar
• Su JM, Perlaky L, Li XN, Leung HC, Antalffy B, Armstrong D, Lau CC. Comparison of ethanol versus formalin on preservation of histology and RNA in laser capture microdissected brain tissues. Brain Pathol. 2004; 14: 175–182
   PubMed Web of Science ®Google Scholar
• Telenius H, Carter NP, Bebb CE, Nordenskjold M, Ponder BA, Tunnacliffe A. Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer. Genomics 1992; 13: 718–725
   PubMed Web of Science ®Google Scholar
• Thompson-Hehir J, Davies MP, Green JA, Halliwell N, Joyce KA, Salisbury J, Sibson DR, Vergote I, Walker C. Novel polymerase chain reaction approach for full-coding p53 mutation detection in microdissected archival tumors. Diagn. Mol. Pathol. 2000; 9: 110–119
   Google Scholar
• Weber RG, Scheer M, Born IA, Joos S, Cobbers JM, Hofele C, Reifenberger G, Zoller JE, Lichter P. Recurrent chromosomal imbalances detected in biopsy material from oral premalignant and malignant lesions by combined tissue microdissection, universal DNA amplification, and comparative genomic hybridization. Am. J. Pathol. 1998; 153: 295–303
   PubMedGoogle Scholar
• Williams C, Ponten F, Moberg C, Soderkvist P, Uhlen M, Ponten J, Sitbon G, Lundeberg J. A high frequency of sequence alterations is due to formalin fixation of archival specimens. Am. J. Pathol. 1999; 155: 1467–1471
   PubMed Web of Science ®Google Scholar
• Zhuang Z, Bertheau P, Emmert-Buck MR, Liotta LA, Gnarra J, Linehan WM, Lubensky IA. A microdissection technique for archival DNA analysis of specific cell populations in lesions < 1 mm in size. Am. J. Pathol. 1995; 146: 620–625
   PubMed Web of Science ®Google Scholar