Advanced search
Publication Cover
Free Radical Research Volume 36, 2002 - Issue 2
Submit an article Journal homepage
169
Views
19
CrossRef citations to date
0
Altmetric
Research Article

Simple Cryoprotection and Cell Dissociation Techniques for Application of the Comet Assay to Fresh and Frozen Rat Tissues

Miao-Lin Hu Department of Food Science, National Chung-Hsing University, 250 Kuo-Kuang Road, Taichung 402, Taiwan
,
Cheng-Hung Chuang Department of Food Science, National Chung-Hsing University, 250 Kuo-Kuang Road, Taichung 402, Taiwan
,
Hok-Man Sio Department of Food Science, National Chung-Hsing University, 250 Kuo-Kuang Road, Taichung 402, Taiwan
&
Shu-Lan Yeh Department of Food Science, National Chung-Hsing University, 250 Kuo-Kuang Road, Taichung 402, Taiwan
Pages 203-209 | Published online: 07 Jul 2009

  • Ames, B.N. and Gold, L.S. (1991) "Endogenous mutagens andthe causes of aging and cancer", Mutat. Res. 250, 3-16.
  • Ames, B.N., Gold, L.S. and Willet, W.C. (1995) "The causesand prevention of cancer", Proc. Natl Acad. Sci. USA 92,5258-5265.
  • Cerutti, P., Larsson, R., Krupitza, G., Muehlmatter, D.,Crawford, D. and Amstad, P. (1989) "Pathophysiologicalmechanisms of active oxygen", Mutat. Res. 214, 81-88.
  • Floyd, R.A. (1990) "The role of 8-deoxyguanine in carcino-genesis", Carcinogenesis 11, 1447-1450.
  • Lindahl, T. and Wood, R.D. (1999) "Quality control by DNArepair", Science 286, 1897-1905.
  • Singh, N.P., McCoy, M.T., Tice, R.R. and Schneider, E.L.A.(1988) "A simple technique for quantitation of low levels ofDNA damage in individual cells", Exp. Cell Res. 175, 184-191.
  • Fairbairn, D.W., Olive, P.L. and O'Neill, K.L. (1995) "Thecomet assay: a comprehensive review", Mutat. Res. 339,37-59.
  • Collins, A.R., Dobson, V.L., Dusinska, M., Kennedy, G. andStetina, R. (1997) "The comet assay: what can it really tellus?", Mutat. Res. 375, 183-193.
  • Halliwell, B. and Gutteridge, J.M.C. (1999) Free Radicals inBiology and Medicine, 3rd ed. (Oxford University Press, NewYork), Figure 4.8, pp 268-269.
  • Monteith, D.K. and Vanstone, J. (1995) "Comparison of themicrogel electrophoresis assay and other assays for geno-toxicity in the detection of DNA damage", Mutat. Res. 345,97-103.
  • Miyamae, Y., Yamamoto, M., Sasaki, Y.F, Kobayashi, H., Igarashi-Soga, M., Shimoi, K. and Hayashi, M. (1998) "Evaluation of a tissue homogenization technique that isolates nuclei for the in vivo single cell gel electrophoresis (comet) assay: a collaborative study by five laboratories", Mutat. Res. 418, 131-140.
  • Tice, R.R., Andrews, P.W. and Singh, N.P. (1990) "The single cell gel electrophoresis assay: a sensitive technique for evaluating intracellular differences in DNA damage and repair", Basic Life Sci. 53, 291-301.
  • Betti, C., Davini, T., Giannessi, L., Loprieno, N. and Barale, R. (1993) "Comparative studies on cytotoxic and genotoxic effects of two organic mercury compounds in lymphocytes and gastric mucosa cells of Sprague-Dawley rats", Environ. MoI. Mutagen. 22, 172-180.
  • Plappert, U., Barthel, E., Raddatz, K. and Seidel, H.J. (1994) "Early effects of benzene exposure in mice. Hematological versus genotoxic effects", Arch. Toxicol. 68, 284-290.
  • Sirota, N.P., Bezlepkin, V.G., Kuznetsova, E.A., Lomayeva, M.G., Milonova, I.M., Ravin, V.K., Gaziev, A.I. and Bradbury, R.J. (1996) "Modifying effect in vivo of interferon on induction and repair of lesions in DNA of lymphoid cells of gammairradiated mice", Radiat. Res. 146, 100-105.
  • Fairbairn, D.W., Reyes, W.A., Grigsby, R.V. and O'Neill, K. (1994) "Laser scanning microscopic analysis of DNA damage in frozen tissues", Cancer Lett. 76, 127-132.
  • Sasaki, Y.E, Tsuda, S., Izumiyama, F. and Nishidate, E. (1997) "Detection of chemically induced DNA lesions in multiple mouse organs (liver, spleen, kidney, and bone marrow) using the single cell gel electrophoresis (Comet) assay", Mutat. Res. 388, 33-44.
  • Sasaki, Y.F., Nishidate, E., Izumiyama, F., Matsusaka, N. and Tsuda, S. (1997) "Simple detection of chemical mutagens by the alkaline single-cell gel electrophoresis (Comet) assay in multiple mouse organs (liver, spleen, kidney, and bone marrow)", Mutat. Res. 391, 215-231.
  • Mazur, P. (1970) "Cryobiology: the freezing of biological systems", Science 168, 939-949.
  • Ebina, Y., Okada, S., Hamazaki, S., Ogino, F,. Li, J.I. and Midorikawa, O. (1986) "Nephrotoxicity and renal cell carcinoma after use of iron- and aluminum nitrilotriacetate complexes in rats", J. Natl Inst. Cancer 76, 107-113.
  • Iqbal, M. and Arthur, M. (1998) "Attenuation of ironnitrilotriacetate (Fe-NTA)-mediated renal oxidative stress, toxicity and hyperproliferative response by the prophylactic treatment of rats with garlic oil", Food Chem. Toxicol. 36, 485-495.
  • Iqbal, M., Giri, U. and Arthur, M. (1995) "Ferric nitrilotriacetate (Fe-NTA) is a potent hepatic tumor promoter and acts through the generation of oxidative stress", Biochem. Biophys. Res. Commun. 212, 557-563.
  • Phillips, H.J. (1973) "Dye exclusion tests for cell viability", In: Kruse, P.E. and Patterson, M.K., eds, Tissue Culture, Method and Applications (Academic Press, New York), pp 406-408.
  • Collins, A.R., Ma, A-G. and Duthie, S.J. (1995) "The kinetics of repair of oxidative DNA damage (strand breaks and oxidized pyrimidines) in human cells", Mutat. Res. 336, 69-77.
  • Olive, P.L., Banath, J.P. and Durand, R.E. (1990) "Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells using the comet assay", Radiat. Res. 122, 86-94.
  • Mckelvey-Martin, V.J., Green, M.H.L., Schmezer, P., PoolZobel, B.L., De Meo, M.P. and Collins, A. (1993) "The single cell gel electrophoresis assay (comet assay): a European review", Mutat. Res. 288, 47-63.
  • Collins, A.R. (1992) "Workshop on single cell gel electrophoresis (the "comet" assay)", Mutagenesis 7, 357-358.
  • Yeh, S.-L. and Hu, M.-L. (2001) "Induction of DNA damage in human foreskin fibroblast Hs68 cells by oxidized ß-carotene and lycopene", Free Radic. Res. 35, 203-213.
  • Cesarone, C.E, Fugassa, E., Gallo, G., Voci, A. and Orunesu, M. (1984) "Collagenase perfusion of rat liver induces DNA damage and DNA repair in hepatocytes", Mutat. Res. 141, 113-116.
  • Chen, C.-C. (1998) "The cryopreservation of animal cell lines", Food Indust. (Taiwan) 30, 44-50.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.