R-Lipoic Acid Inhibits Mammalian Pyruvate Dehydrogenase Kinase

References

• Bustamante, J., Lodge, J.K., Marcocci, L., Tritschler, H.J., Packer, L. and Rihn, B.H. (1998) "Alpha-lipoic acid in liver metabolism and disease", Free Radic. Biol. Med. 24, 1023–1039.
   PubMed Web of Science ®Google Scholar
• Patel, M.S. and Hong, YS. (1998) "Lipoic acid as an antioxidant. The role of dihydrolipoamide dehydrogenase", Methods Mol. Biol. 108, 337–346.
   PubMedGoogle Scholar
• Carreau, J.P. (1979) "Biosynthesis of lipoic acid via unsatu-rated fatty acids", Methods Enzymol. 62, 152–158.
   PubMedGoogle Scholar
• Prasad, PD., Wang, H., Kekuda, R., Fujita, T., Fei, Y.J., Devoe, L.D., Leibach, F.H. and Ganapathy, V. (1998) "Cloning and functional expression of a cDNA encoding a mammalian sodium-dependent vitamin transporter mediating the uptake of pantothenate, biotin, and lipoate", J. Biol. Chem. 273, 7501–7506.
   PubMed Web of Science ®Google Scholar
• Moini, H., Packer, L. and Saris, N.E. (2002) "Antioxidant and prooxidant activities of alpha-lipoic acid and dihydrolipoic acid", Toxicol. Appl. Pharmacol. 182, 84–90.
   PubMed Web of Science ®Google Scholar
• Kagan, V.E., Serbinova, E.A., Forte, T., Scita, G. and Packer, L. (1992) "Recycling of vitamin E in human low density lipoproteins", J. Lipid Res. 33, 385–397.
   PubMed Web of Science ®Google Scholar
• Gotz, M.E., Dirr, A., Burger, R., Janetzky, B., Weinmuller, M., Chan, W.W., Chen, S.C., Reichmann, H., Rausch, W.D. and Riederer, P. (1994) "Effect of lipoic acid on redox state of coenzyme Q in mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and diethyldithiocarbamate", Fur. J. Pharmacol. 266, 291–300.
   Google Scholar
• Jocelyn, P.C. (1967) "The standard redox potential of cysteine-cystine from the thiol-disulphide exchange reaction with glutathione and lipoic acid", Fur. J. Biochem. 2, 327–331.
   Web of Science ®Google Scholar
• Haak, E.S., Usadel, K.H., Kohleisen, M., Yilmaz, A., Kusterer, K. and Haak, T. (1999) "The effect of alpha-lipoic acid on the neurovascular reflex arc in patients with diabetic neuropathy assessed by capillary microscopy", Microvasc. Res. 58, 28–34.
   PubMed Web of Science ®Google Scholar
• Yaworsky, K., Somwar, R., Ramlal, T., Tritschler, H.J. and Klip, A. (2000) "Engagement of the insulin-sensitive pathway in the stimulation of glucose transport by alpha-lipoic acid in 3T3-L1 adipocytes", Diabetologia 43, 294–303.
   PubMed Web of Science ®Google Scholar
• Konrad, D., Somwar, R., Sweeney, G., Yaworsky, K., Hayashi, M., Ramlal, T. and Klip, A. (2001) "The antihyperglycemic drug alpha-lipoic acid stimulates glucose uptake via both GLUT4 translocation and GLUT4 activation: potential role of p38 mitogen-activated protein kinase in GLUT4 activation", Diabetes 50, 1464–1471.
   PubMed Web of Science ®Google Scholar
• Moini, H., Tirosh, 0., Park, Y.C., Cho, K.J. and Packer, L. (2002) "R-alpha-lipoic acid action on cell redox status, the insulin receptor, and glucose uptake in 3T3-L1 adipocytes", Arch. Biochem. Biophys. 397, 384–391.
   Google Scholar
• Konrad, T., Vicini, P., Kusterer, K., Hoflich, A., Assadkhani, A., Bohles, H.J., Sewell, A., Tritschler, H.J., Cobelli, C. and Usadel, K.H. (1999) "Alpha-Lipoic acid treatment decreases serum lactate and pyruvate concentrations and improves glucose effectiveness in lean and obese patients with type 2 diabetes", Diabetes Care 22, 280–287.
   PubMed Web of Science ®Google Scholar
• Hagen, T.M., Ingersoll, R.T., Lykkesfeldt, J., Liu, J., Wehr, C.M., Vinarsky, V, Bartholomew, J.C. and Ames, A.B. (1999) "(R)-alpha-lipoic acid-supplemented old rats have improved mitochondrial function, decreased oxidative damage, and increased metabolic rate", FASEB J. 13, 411–418.
   PubMed Web of Science ®Google Scholar
• Patel, US. and Korotchkina, L.G. (2003) "The biochemistry of the pyruvate dehydrogenase complex", Biochem. Mol. Biol. Educ. 31, 5–15.
   Web of Science ®Google Scholar
• Zhou, Z.H., McCarthy, D.B., O'Connor, C.M., Reed, L.J. and Stoops, J.K. (2001) "The remarkable structural and functional organization of the eukaryotic pyruvate dehydrogenase complexes", Proc. Natl Acad. Sci. USA 98, 14802–14807.
   PubMed Web of Science ®Google Scholar
• Yeaman, S.J., Hutcheson, E.T., Roche, T.E., Pettit, F.H., Brown, J.R., Reed, L.J., Watson, D.C. and Dixon, G.H. (1978) "Sites of phosphorylation on pyruvate dehydrogenase from bovine kidney and heart", Biochemistry 17, 2364–2370.
   PubMed Web of Science ®Google Scholar
• Gudi, R., Bowker-Kinley, M.M., Kedishvili, N.Y., Zhao, Y. and Popov, K.M. (1995) "Diversity of the pyruvate dehydrogenase kinase gene family in humans", J. Biol. Chem. 270, 28989–28994.
   PubMed Web of Science ®Google Scholar
• Rowles, J., Scherer, S.W., Xi, T., Majer, M., Nickle, D.C., Rommens, J.M., Popov, K.M., Harris, R.A., Riebow, N.L., Xia, J., Tsui, L.C., Bogardus, C. and Prochazka, M. (1996) "Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme inhuman", J. Biol. Chem. 271, 22376 —22382.
   Web of Science ®Google Scholar
• Huang, B., Gudi, R., Wu, P., Harris, R.A., Hamilton, J. and Popov, K.M. (1998) "Isoenzymes of pyruvate dehydrogenase phosphatase. DNA-derived amino acid sequences, expression and regulation", J. Biol. Chem. 273, 17680–17688.
   PubMed Web of Science ®Google Scholar
• Bowker-Kinley, M.M., Davis, W.I., Wu, P., Harris, R.A. and Popov, K.M. (1998) "Evidence for existence of tissue-specific regulation of the mammalian pyruvate dehydrogenase complex", Biochem. J. 329, 191–196.
   PubMed Web of Science ®Google Scholar
• Roche, T.E., Baker, J.C., Yan, X., Hiromasa, Y., Gong, X., Peng, T., Dong, J., Turkan, A. and Kasten, S.A. (2001) "Distinct regulatory properties of pyruvate dehydrogenase kinase and phosphatase isoforms", Progr. Nucl. Acid Res. Mol. Biol. 70, 33–75.
   PubMed Web of Science ®Google Scholar
• Sugden, M.C. and Holness, M.J. (2003) "Recent advances in mechanisms regulating glucose oxidation at the level of the pyruvate dehydrogenase complex by PDKs", Am. J. Physiol. Endocrinol. Metab. 284, E855—E862.
   Web of Science ®Google Scholar
• Estrada, D.E., Ewart, H.S., Tsakiridis, T., Volchuk, A., Ramlal, T., Tritschler, H. and Klip, A. (1996) "Stimulation of glucose uptake by the natural coenzyme alpha-lipoic acid/ thioctic acid: participation of elements of the insulin signaling pathway", Diabetes 45, 1798–1804.
   PubMed Web of Science ®Google Scholar
• Gandhi, V.M., Wagh, S.S., Natraj, CV. and Menon, K.K.G. (1985) "Lipoic acid and diabetes II: Mode of action of lipoic acid", J. Biosci. 9, 117–127.
   Web of Science ®Google Scholar
• Walgren, J.L., Amani, Z., McMillan, J.M., Locher, M. and Buse, M.G. (2004) "Effect of R(+)alpha-lipoic acid on pyruvate metabolism and fatty acid oxidation in rat hepatocytes", Metabolism 53, 165–173.
   PubMed Web of Science ®Google Scholar
• Korotchkina, L.G., Tucker, M.M., Thekkumkara, T.J., Madhu-sudhan, K.T., Pons, G., Kim, H. and Patel, M.S. (1995) "Overexpression and characterization of human tetrameric pyruvate dehydrogenase and its individual subunits", Protein Expr. Purif 6, 79–90.
   PubMed Web of Science ®Google Scholar
• Korotchkina, L.G. and Patel, M.S. (1995) "Mutagenesis studies of the phosphorylation sites of recombinant human pyruvate dehydrogenase. Site-specific regulation", J. Biol. Chem. 270, 14297–14304.
   PubMed Web of Science ®Google Scholar
• Korotchkina, L.G. and Patel, US. (2001) "Probing the mechanism of inactivation of human pyruvate dehydroge-nase by phosphorylation of three sites", J. Biol. Chem. 276, 5731 —5738.
   Web of Science ®Google Scholar
• Liu, T.-C., Korotchkina, L.G., Hyatt, S., Vettakkorumakankav, N.N. and Patel, M.S. (1995) "Spectroscopic studies of the characterization of recombinant human dihydrolipoamide dehydrogenase and its site-directed mutants", J. Biol. Chem. 270, 15545–15550.
   PubMed Web of Science ®Google Scholar
• Bradford, M.M. (1976) "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal. Biochem. 72, 248–254.
   PubMed Web of Science ®Google Scholar
• Yang, D., Gong, X., Yakhnin, A. and Roche, T.E. (1998) "Requirements for the adaptor protein role of dihydrolipoyl acetyltransferase in the up-regulated function of the pyruvate dehydrogenase kinase and pyruvate dehydrogenase phos-phatase", J. Biol. Chem. 273, 14130–14137.
   PubMed Web of Science ®Google Scholar
• Korotchkina, L.G. and Patel, US. (2001) "Site specificity of four pyruvate dehydrogenase kinase isoenzymes toward the three phosphorylation sites of human pyruvate dehydro-genase", J. Biol. Chem. 276, 37223–37229.
   PubMed Web of Science ®Google Scholar
• Ravindran, S., Radke, G.A., Guest, J.R. and Roche, T.E. (1996) "Lipoyl domain-based mechanism for the integrated feed-back control of the pyruvate dehydrogenase complex by enhancement of pyruvate dehydrogenase kinase activity", J. Biol. Chem. 271, 653–662.
   PubMed Web of Science ®Google Scholar
• Jacobia, S.J., Korotchkina, L.G. and Patel, M.S. (2001) "Differential effects of two mutations at arginine-234 in the alpha subunit of human pyruvate dehydrogenase", Arch. Biochem. Biophys. 395, 121–128.
   PubMed Web of Science ®Google Scholar
• Pratt, M.L. and Roche, T.E. (1979) "Mechanism of pyruvate inhibition of kidney pyruvate dehydrogenase kinase and synergistic inhibition by pyruvate and ADP", J. Biol. Chem. 254, 7191–7196.
   PubMed Web of Science ®Google Scholar
• Thelen, J.J., Miernyk, J.A. and Randall, D.D. (2000) "Pyruvate dehydrogenase kinase from Arabidopsis thaliana: a protein histidine kinase that phosphorylates serine residues", Biochem. J. 349, 195–201.
   PubMed Web of Science ®Google Scholar
• Ciszak, EM., Korotchkina, L.G., Dominiak, P.M., Sidhu, S. and Patel, US. (2003) "Structural basis for flip-flop action of thiamin pyrophosphate-dependent enzymes revealed by human pyruvate dehydrogenase", J. Biol. Chem. 278, 21240–21246.
   PubMed Web of Science ®Google Scholar
• Pettit, F.H., Humphreys, J. and Reed, L.J. (1982) "Regulation of pyruvate dehydrogenase kinase activity by protein thiol-disulfide exchange", Proc. Natl Acad. Sci. USA 79, 3945–3948.
   PubMed Web of Science ®Google Scholar
• Steussy, C.N., Popov, KM., Bowker-Kinley, UM., Sloan, R.B., Jr., Harris, R.A. and Hamilton, J.A. (2001) "Structure of pyruvate dehydrogenase kinase. Novel folding pattern for a serine protein kinase", J. Biol. Chem. 276, 37443–37450.
   PubMed Web of Science ®Google Scholar
• Mann, W.R., Dragland, C.J., Vinluan, C.C., Vedananda, T.R., Bell, P.A. and Aicher, T.D. (2000) "Diverse mechanisms of inhibition of pyruvate dehydrogenase kinase by structurally distinct inhibitors", Biochim. Biophys. Acta 1480, 283–292.
   PubMed Web of Science ®Google Scholar
• Mayers, R.M., Butlin, R.J., Kilgour, E., Leighton, B., Martin, D., Myatt, J., Orme, J.P. and Holloway, B.R. (2003) "AZD7545, a novel inhibitor of pyruvate dehydrogenase kinase 2 (PDHK2), activates pyruvate dehydrogenase in vivo and improves blood glucose control in obese (fa/fa) Zucker rats", Biochem. Soc. Trans. 31, 1165–1167.
   PubMed Web of Science ®Google Scholar
• Morrell, J.A., Orme, J., Butlin, R.J., Roche, T.E., Mayers, R.M. and Kilgour, E. (2003) "AZD7545 is a selective inhibitor of pyruvate dehydrogenase kinase 2", Biochem. Soc. Trans. 31, 1168–1170.
   PubMed Web of Science ®Google Scholar
• Huang, B., Wu, P., Popov, KM. and Harris, R.A. (2003) "Starvation and diabetes reduce the amount of pyruvate dehydrogenase phosphatase in rat heart and kidney", Diabetes 52, 1371–1376.
   PubMed Web of Science ®Google Scholar
• Harris, R.A., Huang, B. and Wu, P. (2001) "Control of pyruvate dehydrogenase kinase gene expression", Adv. Enzyme Regul. 41, 269–288.
   PubMedGoogle Scholar
• Wu, P., Sato, J., Zhao, Y., Jaskiewicz, J., Popov, K.M. and Harris, R.A. (1998) "Starvation and diabetes increase the amount of pyruvate dehydrogenase kinase isoenzyme 4 in rat heart", Biochem. J. 329, 197–201.
   PubMed Web of Science ®Google Scholar
• Wu, P., Blair, P.V., Sato, J., Jaskiewicz, J., Popov, K.M. and Harris, R.A. (2000) "Starvation increases the amount of pyruvate dehydrogenase kinase in several mammalian tissues", Arch. Biochem. Biophys. 381, 1–7.
   PubMed Web of Science ®Google Scholar
• Sugden, MC., Lall, H.S., Harris, R.A. and Holness, M.J. (2000) "Selective modification of the pyruvate dehydrogenase kinase isoform profile in skeletal muscle in hyperthyroidism: implications for the regulatory impact of glucose on fatty acid oxidation", J. Endocrinol. 167, 339–345.
   PubMed Web of Science ®Google Scholar