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Free Radical Research Volume 55, 2021 - Issue 6
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Original Articles

In-gel SOD assay reveals SOD-2 is the single active, water-soluble SOD enzyme in C. elegans

Lourds M. Fernandoa Department of Biology, Howard University, Washington, DC, USA;b St. Jude Children's Research Hospital, Memphis, Tennessee, USAView further author information
,
Silwat Adeela Department of Biology, Howard University, Washington, DC, USAView further author information
,
Mohammed Abul Basara Department of Biology, Howard University, Washington, DC, USA;c Stem Cell Biochemistry Unit, National Institute of Dental and Craniofacial Research National Institutes of Health, Bethesda, MD, USAView further author information
,
Anna K. Allena Department of Biology, Howard University, Washington, DC, USAView further author information
&
Atanu Duttaroya Department of Biology, Howard University, Washington, DC, USACorrespondence[email protected]
View further author information
Pages 619-624 | Received 29 Apr 2020, Accepted 07 Sep 2021, Published online: 26 Oct 2021

References

  • Fridovich I. Superoxide radical and superoxide dismutases. Annu Rev Biochem. 1995;64:97–112.
  • Weisiger RA, Fridovich I. Superoxide dismutase: organelle specificity. J Biol Chem. 1973;248(13):4793–4796.
  • Missirlis F, Hu J, Kirby K, et al. Compartment- specific protection of iron–sulfur proteins by superoxide dismutase. J Biol Chem. 2003;278(48):47365–47369.
  • Muller FL, Liu Y, Van Remmen H. Complex III releases superoxide to both sides of the inner mitochondrial membrane. J Biol Chem. 2004;279(47):49064–49073.
  • Lebovitz RM, Zhang H, Vogel H, et al. Neurodegeneration, myocardial injury, and perinatal death in mitochondrial superoxide dismutase-deficient mice. Proc Natl Acad Sci USA. 1996;93(18):9782–9787.
  • Li Y, Huang TT, Carlson EJ, et al. Dilated cardiomyopathy and neonatal lethality in mutant mice lacking manganese superoxide dismutase. Nat Genet. 1995;11(4):376–381.
  • Duttaroy A, Paul A, Kundu M, et al. A Sod2 null mutation confers severely reduced adult life span in drosophila. Genetics. 2003;165(4):2295–2299.
  • Kirby K, Hu J, Hilliker AJ, Phillips JP. RNA interference-mediated silencing of Sod2 in Drosophila leads to early adult-onset mortality and elevated endogenous oxidative stress. Proc Natl Acad Sci USA. 2002;99(25):16162–16167.
  • Paul A, Belton A, Nag S, et al. Reduced mitochondrial SOD displays mortality characteristics reminiscent of natural aging. Mech Ageing Dev. 2007;128(11–12):706–716.
  • Godenschwege T, Forde R, Davis CP, et al. Mitochondrial superoxide radicals differentially affect muscle activity and neural function. Genetics. 2009;183(1):175–184.
  • Culotta VC, Yang M, O'Halloran TV. Activation of superoxide dismutases: putting the metal to the pedal. Biochim Biophys Acta. 2006;1763(7):747–758.
  • Valentine JS, Doucette PA, Potter SZ. Copper-zinc superoxide dismutase and amyotrophic lateral sclerosis. Annu Rev Biochem. 2005;74:563–593.
  • Phillips JP, Campbell SD, Michaud D, et al. Null mutation of copper/zinc superoxide dismutase in drosophila confers hypersensitivity to paraquat and reduced longevity. Proc Natl Acad Sci USA. 1989;86(8):2761–2765.
  • Culotta VC. Superoxide dismutase, oxidative stress, and cell metabolism. Curr Top Cell Regul. 2000;36:117–132.
  • Corsi AK, Wightman B, Chalfie M. A transparent window into biology: a primer on Caenorhabditis elegans. Genetics. 2015;200(2):387–407.
  • Landis GN, Tower J. Superoxide dismutase evolution and life span regulation. Mech Ageing Dev. 2005;126(3):365–379.
  • Doonan R, McElwee JJ, Matthijssens F, et al. Against the oxidative damage theory of aging: superoxide dismutases protect against oxidative stress but have little or no effect on life span in Caenorhabditis elegans. Genes Dev. 2008;22(23):3236–3241.
  • Raamsdonk JM, Hekimi S. Superoxide dismutase is dispensable for normal animal lifespan. Proc Natl Acad Sci USA. 2012;109(15):5785–5790.
  • Hunter T, Bannister WH, Hunter GJ. Cloning, expression, and characterization of two manganese superoxide dismutases from Caenorhabditis elegans. J Biol Chem. 1997;272(45):28652–28659.
  • Jensen LT, Culotta VC. Activation of CuZn superoxide dismutases from Caenorhabditis elegans does not require the copper chaperone CCS. J Biol Chem. 2005;280(50):41373–41379.
  • Sakamoto T, Imai H. Hydrogen peroxide produced by superoxide dismutase SOD-2 activates sperm in Caenorhabditis elegans. J Biol Chem. 2017;292(36):14804–14813.
  • Weydert CJ, Cullen JJ. Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nat Protoc. 2010;5(1):51–66.
  • Honda Y, Honda S. The daf-2 gene network for longevity regulates oxidative stress resistance and Mn-superoxide dismutase gene expression in Caenorhabditis elegans. Faseb J. 1999;13(11):1385–1393.
  • Hunter GJ, Trinh CH, Bonetta R, et al. The structure of the Caenorhabditis elegans manganese superoxide dismutase MnSOD-3-azide complex. Protein Sci. 2015;24(11):1777–1788.
  • Sheng Y, Abreu IA, Cabelli DE, et al. Superoxide dismutases and superoxide reductases. Chem Rev. 2014;114(7):3854–3918.
  • Fujii M, Ishi N, Joguchi A, et al. A novel superoxide dismutase gene encoding membrane-bound and extracellular isoforms by alternative splicing in Caenorhabditis elegans. DNA Res. 1998;5(1):25–30.
  • Van Raamsdonk JM, Hekimi S. Deletion of the mitochondrial superoxide dismutase sod-2 extends lifespan in Caenorhabditis elegans. PLOS Genet. 2009;5(2):e1000361.
  • Mehta R, Steinkraus KA, Sutphin GL, et al. Proteasomal regulation of the hypoxic response modulates aging in C. elegans. Science. 2009;324(5931):1196–1198.

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