Design, recombinant expression, and antibacterial activity of a novel hybrid magainin–thanatin antimicrobial peptide

References

• Rather, I.A.; Kim, B.C.; Bajpai, V.K.; Park, Y.H. Self-Medication and Antibiotic Resistance: Crisis, Current Challenges, and Prevention. Saudi J. Biol. Sci. 2017, 24(4), 808–812.
   PubMed Web of Science ®Google Scholar
• Mackenzie, D. Antibiotic Resistance Hits Crisis Point. New Sci. 2016, 232(32), 3104–3106.
   Google Scholar
• Rossolini, G.M.; Arena, F.; Pecile, P.; Pollini, S. Update on the Antibiotic Resistance Crisis. Curr. Opin. Pharmaco. 2014, 18, 56–60.
   PubMed Web of Science ®Google Scholar
• Małgorzata, J.B.; Kamila, S.S. Peptides having Antimicrobial Activity and their Complexes with Transition Metal Ions. Eur. J. Med. Chem. 2018, 143, 997–1009.
   PubMed Web of Science ®Google Scholar
• Brogden, K.A. Antimicrobial Peptides: Pore Formers or Metabolic Inhibitors in Bacteria. Nat. Rev. Microbiol. 2005, 3(3) 238–250.
   PubMed Web of Science ®Google Scholar
• Wieprecht, T.; Dathe, M.; Beyermann, M.; Krause, E.; Maloy, W.L.; MacDonald, D.L.; Bienert, M. Peptide Hydrophobicity Controls the Activity and Selectivity of Magainin 2 Amide in Interaction with Membranes. Biochem. 1997, 36(20), 6124–6132.
   PubMed Web of Science ®Google Scholar
• Zasloff, M. Magainins, A Class of Antimicrobial Peptides from Xenopus Skin: Isolation, Characterization of Two Active Forms, and Partial cDNA Sequence of a Precursor. Proc. Natl. Acad. Sci. USA. 1987, 84(15), 5449–5449.
   Web of Science ®Google Scholar
• Bulet, P.; Hetru, C.; Dimarcq, J.L.; Hoffmann, D. Antimicrobial Peptides in Insects: Structure and Function. Dev. Comp. Immunol. 1999. 23(4–5), 329–344.
   PubMed Web of Science ®Google Scholar
• Ladokhin, A.S.; Selsted, M.E.; White, S.H. CD Spectra of Indolicidin Antimicrobial Peptides suggest Turns, Not Polyproline Helix. Biochem. 1999, 38(38), 12313–12319.
   PubMed Web of Science ®Google Scholar
• Lamberty, M.; Zachary, D.; Lanot, R.; Bordereau, C.; Robert, A.; Hoffman, J.A.; Bulet, P. Insect Immunity – Constitutive Expression of a Cysteine-rich Antifungal and a Linear Antibacterial Peptide in a Termite Insect. J. Biol. Chem. 2001, 276(6), 4085–4092.
   PubMed Web of Science ®Google Scholar
• Mandard, N.; Sodano, P.; Labbe, H.; Bonmatin, J.M.; Bulet, P.; Hetru, C.; Ptak, M.; Vovelle, F. Solution Structure of Thanatin, A Potent Bactericidal and Fungicidal Insect Peptide, Determined from Proton Two-Dimensional Nuclear Magnetic Resonance Data. Eur. J. Biochem. 1998, 256(2), 404–410.
   PubMedGoogle Scholar
• Fehlbaum, P.; Bulet, P.; Chernysh, S.; Briand, J.P.; Roussel, J.P.; Letellier, L.; Hetru, C.; Hoffmann, J. Structure–Activity Analysis of Thanatin, A 21-Residue Inducible Insect Defense Peptide with Sequence Homology to Frog Skin Antimicrobial Peptides. Proc. Natl. Acad. Sci. USA. 1996, 93(3), 1221–1225.
   PubMed Web of Science ®Google Scholar
• Deng, T.; Ge, H.R.; He, H.H.; Liu,Y.; Zhai, C.; Feng, L.; Yi. L. The Heterologous Expression Strategies of Antimicrobial Peptides in Microbial Systems. Protein Expres. Purif. 2017, 140, 52–59.
   PubMed Web of Science ®Google Scholar
• Qu, H.; Chen, B.; Peng, H.; Wang, K. Molecular Cloning, Recombinant Expression, and Antimicrobial Activity of EC-Hepcidin3, A New Four-Cysteine Hepcidin Isoform from Epinephelus coioides. Biosci. Biotechnol. Biochem. 2013, 77(1), 103–110.
   PubMed Web of Science ®Google Scholar
• Cereghino, G.P.; Cereghino, J.L.; Ilgen, C.; Cregg, J.M. Production of Recombinant Proteins in Fermenter Cultures of the Yeast Pichia pastoris. Curr. Opin. Biotechnol. 2002, 13(4), 329–332.
   PubMed Web of Science ®Google Scholar
• Huang, L.; Ching, C.B.; Jiang, R.R.; Leong, S.S.J. Production of Bioactive Human Beta-Defensin 5 and 6 in Escherichia coli by Soluble Fusion Expression. Protein Expr. Purif. 2008, 61(2), 168–174.
   PubMed Web of Science ®Google Scholar
• Kane, J.F. Effects of Rare Codon Clusters on High-Level Expression of Heterologous Proteins in Escherichia coli. Curr. Opin. Biotechnol. 1995, 6(5), 494–500.
   PubMed Web of Science ®Google Scholar
• Zhou, Y.; Cao, W.; Wang, J.; Ma, Y.; Wei, D. Comparison of Expression of Monomeric and Multimeric Adenoregulin Genes in Escherichia coli and Pichia pastoris. Protein Pept. Lett. 2005, 12(4), 349–355.
   PubMed Web of Science ®Google Scholar
• Ferre, R.; Melo, M.N.; Correia, A.D.; Feliu, L.; Bardaji, E.; Planas, M.; Castanho, M. Synergistic Effects of the Membrane Actions of Cecropin–Melittin Antimicrobial Hybrid Peptide BP100. Biophys. J. 2009, 96(5), 1815–1827.
   PubMed Web of Science ®Google Scholar
• Chen, X.; Zhu, F.; Cao, Y.; Qiao, S. Novel Expression Vector for Secretion of Cecropin AD in Bacillus subtilis with Enhanced Antimicrobial Activity. Antimicrob. Agents Ch. 2009, 53(9), 3683–3689.
   PubMed Web of Science ®Google Scholar
• Sruthy, K.S.; Nair, A.; Puthumana, J.; Antony, S.P.; Singh, I.S.B.; Philip, R. Molecular Cloning, Recombinant Expression and Functional Characterization of an Antimicrobial Peptide, Crustin from the Indian White Shrimp, Fenneropenaeus indicus. Fish Shellfish Immunol. 2017, 71, 83–94.
   PubMed Web of Science ®Google Scholar
• Ashcheulova, D.O.; Efimova, L.V.; Lushchyk, A.Y.; Yantsevich, A.V.; Baikov, A.N.; Pershina, A.G. Production of the Recombinant Antimicrobial Peptide UBI18-35 in Escherichia coli. Protein Expres. Purif. 2017, 143, 38–44.
   PubMed Web of Science ®Google Scholar
• Di, X.; Da T.; Xiu, M.W.; Ruo, Y.M.; Ya, L.Y.; Wen, S.X.; Jian, H.W. Design, Expression and Characterization of the Hybrid Antimicrobial Peptide LHP7, Connected by a Flexible Linker, Against Staphylococcus and Streptococcus. Process Biochem. 2013, 48(3), 453–461.
   Web of Science ®Google Scholar
• Cao, Y.; Yu, R.Q.; Liu, Y.; Zhou, H.X.; Song, L.L.; Cao, Y.; Qiao, D.R. Design, Recombinant Expression, and Antibacterial Activity of the Cecropins–Melittin Hybrid Antimicrobial Peptides. Curr. Microbio. 2010, 61(3), 169–175.
   PubMed Web of Science ®Google Scholar
• Gasteiger, E.; Hoogland, C.; Gattiker, A.; Duvaud, S.; Wilkins, M.R.; Appel, R.D.; Bairoch, A. Protein Identification and Analysis Tools on the ExPASy Server. In The Proteomics Protocols Handbook. 1999; pp. 531–542. Humana Press, Passaic County, New Jersey, United States.
   Google Scholar
• Gasteiger, E.; Gattiker, A.; Hoogland, C.; Ivanyi, I.; Appel, R.D.; Bairoch, A. ExPASy: The Proteomics Server for In-depth Protein Knowledge and Analysis. Nucleic Acids Res. 2003, 31(13), 3784–3788.
   PubMed Web of Science ®Google Scholar
• Schneider, M.; Tognolli, M.; Bairoch, A. The Swiss-Prot Protein Knowledgebase and ExPASy: Providing the Plant Community with High Quality Proteomic Data and Tools. Plant Physiol. Bioch. 2004, 42(12), 1013–1021.
   PubMed Web of Science ®Google Scholar
• Hoogland, C.; Mostaguir, K.; Appel, R.D.; Lisacek, F. The World-2DPAGE Constellation to Promote and Publish Gel-based Proteomics Data Through the ExPASy Server. J. Proteomics. 2008, 71(2), 245–248.
   PubMed Web of Science ®Google Scholar
• Geourjon, C.; Deleage, G. SOPM: A Self-Optimized Method for Protein Secondary Structure Prediction. Protein Eng. 1994, 7(2), 157–164.
   PubMedGoogle Scholar
• Geourjon, C.; Deleage, G. GSOPMA: Significant Improvements in Protein Secondary Structure Prediction by Consensus Prediction from Multiple Alignments. Bioinformatics. 1995, 11(6), 681–684.
   Google Scholar
• Rice, P.; Longden, I.; Bleasby, A. EMBOSS: The European Molecular Biology Open Software Suite. Trends Genet. 2000, 16(6), 276–277.
   PubMed Web of Science ®Google Scholar
• Spagnoli, G.; Bolchi, A.; Cavazzini, D.; Pouyanfard, S.; Müller, M.; Ottonello, S. Secretory Production of Designed Multipeptides Displayed on a Thermostable Bacterial Thioredoxin Scaffold in Pichia pastoris. Protein Expres. Purif. 2017, 129, 150–157.
   PubMed Web of Science ®Google Scholar
• Tan, L.; Chen, H.J.; Yu, S.Q.; Qiu, X.S.; Song, C.P.; Chen, D.Q.; Zhang, S.L.; Zhang, F.Q.; He, S.B.; Shen, X.Y.; Hu, M.R.; Ding, C. A SOE-PCR Method of Introducing Multiple Mutations into Mycoplasma gallisepticum neuraminidase. J Microbiol. Methods. 2013, 94(2), 117–120.
   PubMed Web of Science ®Google Scholar
• Marius, K.L.; Bruno, M. Analysis of Polypeptides by Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Alongside In Vitro-Generated Reference Peptides. Anal. Biochem. 2003, 319(2), 327–331.
   PubMed Web of Science ®Google Scholar
• Huang, Y.; Huang, J.; Chen, Y. Alpha-Helical Cationic Antimicrobial Peptides: Relationships of Structure and Function. Protein Cell. 2010, 1(2), 143–152.
   PubMed Web of Science ®Google Scholar
• Epand, R.M.; Epand, R.F. Lipid Domains in Bacterial Membranes and The Action of Antimicrobial Agents. BBA-Biomembranes. 2009, 1788(1), 289–294.
   PubMed Web of Science ®Google Scholar
• Yin, L.M.; Edwards, M.A.; Li, J.; Yip, C.M.; Deber, C.M. Roles of Hydrophobicity and Charge Distribution of Cationic Antimicrobial Peptides in Peptide–Membrane Interactions. J. Biol. Chem. 2012, 287(10), 7738–7745.
   PubMed Web of Science ®Google Scholar
• Nasiri, S. S.; Hajizadeh, N.; Alizadeh, H. Antibacterial Effects of Aqueous and Organic Quince Leaf Extracts on Gram-Positive and Gram-Negative Bacteria. Banat's J. Biotech. 2017, 8(16), 54–61.
   Web of Science ®Google Scholar
• Zhang, L.; Benz, R.; Hancock, R.E. Influence of Proline Residues on the Antibacterial and Synergistic Activities of Alphahelical Peptides. Biochem. 1999, 38, 8102–8111.
   PubMed Web of Science ®Google Scholar
• Liu, B.; Li, G.; Sui, X.; Yin, J.; Wang, H.; Ren, X. Expression and Functional Analysis of Porcine Aminopeptidase N Produced in Prokaryotic Expression System. J. Biotech. 2009, 141(1–2), 91–96.
   PubMed Web of Science ®Google Scholar
• Cao, Y.; Yu, R.Q.; Liu, Y.; Zhou, H.X.; Song, L.L.; Cao, Y.; Qiao, D.R. Design, Recombinant Expression, and Antibacterial Activity of the Cecropins–Melittin Hybrid Antimicrobial Peptides. Curr. Microbial. 2010, 61(3), 169–175.
   PubMed Web of Science ®Google Scholar
• Ji, S.; Li, W.; Baloch, A.R.; Wang, M.; Li, H.; Cao, B.; Zhang, H. Efficient Biosynthesis of a Cecropin A–Melittin Mutant in Bacillus subtilis WB700. Sci. Rep. 2017, 7, 40587.
   PubMed Web of Science ®Google Scholar