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Designing an ELP-intein system: toward a more realistic outlook

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References

  • Pavlou, A.K.; Reichert, J.M. Recombinant Protein Therapeutics–Success Rates, Market Trends and Values to 2010. Nat. Biotechnol. 2004, 22, 1513.
  • Mee, C.; Banki, M. R.; Wood, D.W. Towards the Elimination of Chromatography in Protein Purification: Expressing Proteins Engineered to Purify Themselves. Chem. Eng. J. 2008, 135, 56–62.
  • Arnau, J.; Lauritzen, C.; Petersen, G.E.; Pedersen, J. Current Strategies for the Use of Affinity Tags and Tag Removal for the Purification of Recombinant Proteins. Protein Expr. Purif. 2006, 48, 1–13.
  • Kalyanpur, M. Downstream Processing in the Biotechnology Industry. Mol. Biotechnol. 2002, 22, 87–98.
  • Meyer, D.E.; Chilkoti, A. Purification of Recombinant Proteins by Fusion with Thermally-Responsive Polypeptides. Nat. Biotechnol. 1999, 17, 1112–1115.
  • Trabbic, ‐Carlson, K.; Liu, L.; Kim, B.; Chilkoti, A. Expression and Purification of Recombinant Proteins from Escherichia coli: Comparison of an Elastin‐like Polypeptide Fusion with an Oligohistidine Fusion. Protein Sci. 2009, 13, 3274–3284.
  • Floss, D.M.; Schallau, K.; Rose-John, S.; Conrad, U.; Scheller, J. Elastin-like Polypeptides Revolutionize Recombinant Protein Expression and Their Biomedical Application. Trends Biotechnol. 2010, 28, 37–45.
  • Meyer D.E.; Chilkoti A. Chapter 18. Protein Purification by Inverse Transition Cycling. Protein-protein interactions: A molecular cloning manual. 2002:329–344.
  • Phan, H.T.; Conrad, U. Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-like Polypeptide Fusions. Int. J. Mol. Sci. 2011, 12, 2808–2821.
  • Meyer, D.E.; Chilkoti, A. Genetically Encoded Synthesis of Protein-Based Polymers with Precisely Specified Molecular Weight and Sequence by Recursive Directional Ligation: examples from the Elastin-like Polypeptide System. Biomacromolecules. 2002, 3, 357–367.
  • Hassouneh, W.; Christensen, T.; Chilkoti, A. Elastin‐like Polypeptides as a Purification Tag for Recombinant Proteins. Curr. Protoc. Protein. Sci. 2010, 6, 1–6.
  • Waugh, D.S. An Overview of Enzymatic Reagents for the Removal of Affinity Tags. Protein Expr. Purif. 2011, 80, 283–293.
  • Banki, M.R.; Wood, D.W. Inteins and Affinity Resin Substitutes for Protein Purification and Scale up. Microb. Cell Fact. 2005, 4, 32
  • Wood, D. W.; Wu, W.; Belfort, G.; Derbyshire, V.; Belfort, M. A Genetic System Yields self-cleaving inteins for bioseparations. Nat. Biotechnol. 1999, 17, 889–892.
  • Elleuche, S.; Pöggeler, S. Inteins, Valuable Genetic Elements in Molecular Biology and Biotechnology. Appl. Microbiol. Biotechnol. 2010, 87, 479–489.
  • McDaniel, J.R.; MacKay, J.A.; Quiroz, F.G.; Chilkoti, A. Recursive Directional Ligation by Plasmid Reconstruction Allows Rapid and Seamless Cloning of Oligomeric Genes. Biomacromolecules. 2010, 11, 944.
  • Desai, S.; Dworecki, B.; Cichon, E. Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel. Anal. Biochem. 2001, 297, 94–98.
  • Sanchez-Garcia, L.; Martín, L.; Mangues, R.; Ferrer-Miralles, N.; Vázquez, E.; Villaverde, A. Recombinant Pharmaceuticals from Microbial Cells: A 2015 Update. Microb. Cell Fact. 2016, 15, 33.
  • Tian, L.; Sun, S.S. A Cost-Effective ELP-Intein Coupling System for Recombinant Protein Purification from Plant Production Platform. PLoS One. 2011, 6, e24183.
  • Banki, M.R.; Liang, F.; Wood, D.W. Simple Bioseparations Using Self-Cleaving Elastin-like Polypeptide Tags. Nat. Methods. 2005, 2, 659
  • Hommelsheim, C.M.; Frantzeskakis, L.; Huang, M.; Ülker, B. PCR Amplification of Repetitive DNA: A Limitation to Genome Editing Technologies and Many Other Applications. Sci. Rep. 2015, 4, 5052.
  • Sallach, R.E.; Conticello, V.P.; Chaikof, E.L. Expression of a Recombinant Elastin‐like Protein in Pichia pastoris. Biotechnol. Progress. 2009, 25, NA–N8.
  • Chu, H.-S.; Lee, K.-H.; Park, J.-E.; Kim, D.-M.; Kim, B.-G.; Won, J.-I. Expression Analysis of an Elastin-like Polypeptide (ELP) in a Cell-Free Protein Synthesis System. Enzyme Microb. Technol. 2010, 46, 87–91.
  • Gustafsson, C.; Govindarajan, S.; Minshull, J. Codon Bias and Heterologous Protein expression. Trends Biotechnol. 2004, 22, 346–353.
  • Rosano, G.L.; Ceccarelli, E. A. Recombinant Protein Expression in Escherichia coli: advances and Challenges. Frontiers in microbiology. 2014 Apr 17; 5, 172.
  • Teulé, F.; Cooper, A.R.; Furin, W.A.; Bittencourt, D.; Rech, E. L.; Brooks, A.; Lewis, R. V. A Protocol for the Production of Recombinant Spider Silk-like Proteins for Artificial Fiber Spinning. Nat. Protoc. 2009, 4, 341–355.
  • Xia, X.-X.; Qian, Z.-G.; Ki, C.S.; Park, Y.H.; Kaplan, D.L.; Lee, S. Y. Native-Sized Recombinant Spider Silk Protein Produced in Metabolically Engineered Escherichia coli Results in a Strong Fiber. Proc. Natl. Acad. Sci. 2010, 107, 14059–14063.
  • Hassouneh, W.; MacEwan, S.R.; Chilkoti, A. Fusions of Elastin-like Polypeptides to Pharmaceutical Proteins. Meth. Enzymol. 2012, 502, 215
  • Chow, D.C.; Dreher, M.R.; Trabbic, ‐Carlson, K.; Chilkoti, A. Ultra‐High Expression of a Thermally Responsive Recombinant Fusion Protein in E. coli. Biotechnol. Prog. 2006, 22, 638–646.
  • Chu H.S.; Park J.E.; Kim D.M.; Kim B.G.; Won J.I. The effects of supplementing specific amino acids on the expression of elastin-like polypeptides (ELPs). Protein expression and purification. 2010, 74, 298–303.
  • Christensen, T.; Trabbic-Carlson, K.; Liu, W.; Chilkoti, A. Purification of Recombinant Proteins from E. coli at Low Expression Levels by Inverse Transition Cycling. Anal. Biochem. 2007, 360, 166.
  • Fong, B.A.; Wood, D.W. Expression and Purification of ELP-Intein-Tagged Target Proteins in High Cell Density E. coli Fermentation. Microb. Cell Fact. 2010, 9, 77
  • Lewis, R.V.; Hinman, M.; Kothakota, S.; Fournier, M. J. Expression and Purification of a Spider Silk Protein: A New Strategy for Producing Repetitive Proteins. Protein Expr. Purif. 1996, 7, 400–406.
  • Fong, B.A.; Wu, W.-Y.; Wood, D.W. The Potential Role of Self-Cleaving Purification Tags in Commercial-Scale Processes. Trends Biotechnol. 2010, 28, 272–279.
  • Wang, L.; Kang, J.H.; Kim, K.H.; Lee, E. Expression of Intein‐Tagged Fusion Protein and Its Applications in Downstream Processing. J. Chem. Technol. Biotechnol. 2010, 85, 11–18.

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