Biochemical characterization of a partially purified protease from Aspergillus terreus 7461 and its application as an environmentally friendly dehairing agent for leather industry

References

• Khambhaty, Y. Applications of Enzymes in Leather Processing. Environ. Chem. Lett. 2020, 18, 747–769. DOI: 10.1007/s10311-020-00971-5.
   Web of Science ®Google Scholar
• Wanyonyi, W. C.; Mulaa, F. J. Alkaliphilic Enzymes and Their Application in Novel Leather Processing Technology for Next-Generation Tanneries. Adv. Biochem. Eng. Biotechnol. 2020, 172, 195–220. DOI: 10.1007/10_2019_95.
   PubMed Web of Science ®Google Scholar
• Khandelwal, H. B.; More, S. V.; Kalal, K. M.; Laxman, R. S. Eco-Friendly Enzymatic Dehairing of Skins and Hides by C. brefeldianus Protease. Clean Techn. Environ. Policy 2015, 17, 393–405. DOI: 10.1007/s10098-014-0791-y.
   Web of Science ®Google Scholar
• Huang, Q.; Peng, Y.; Li, X.; Wang, H.; Zhang, Y. Purification and Characterization of an Extracellular Alkaline Serine Protease with Dehairing Function from Bacillus pumilus. Curr. Microbiol. 2003, 46, 169–173. DOI: 10.1007/s00284-002-3850-2.
   PubMed Web of Science ®Google Scholar
• Abu-Tahon, M. A.; Arafat, H. H.; Isaac, G. S. Laundry Detergent Compatibility and Dehairing Efficiency of Alkaline Thermostable Protease Produced from Aspergillus terreus Under Solid-State Fermentation. J. Oleo Sci. 2020, 69, 241–254. DOI: 10.5650/jos.ess19315. J.
   PubMed Web of Science ®Google Scholar
• Sharma, M.; Gat, Y.; Arya, S.; Kumar, V.; Panghal, A.; Kumar, A. A Review on Microbial Alkaline Protease: An Essential Tool for Various Industrial Approaches. Ind. Biotechnol 2019, 15, 69–78. DOI: 10.1089/ind.2018.0032.
   Google Scholar
• Zekeya, N.; China, C.; Mbwana, S.; Mtambo, M. Dehairing of Animal Hides and Skins by Alkaline Proteases of Aspergillus oryzae for Efficient Processing to Leather Products in Tanzania. Afr. J. Biotechnol. 2019, 18, 426–434. DOI: 10.5897/AJB2019.16752.
   Google Scholar
• Sharma, K. M.; Kumar, R.; Panwar, S.; Kumar, A. Microbial Alkaline Proteases: Optimization of Production Parameters and Their Properties. J. Genet. Eng. Biotechnol. 2017, 15, 115–116. DOI: 10.1016/j.jgeb.2017.02.001.
   PubMedGoogle Scholar
• Neto, J. M. W. D.; Wanderley, M. C. A.; Lima, C. A.; Porto, A. L. F. Single Step Purification via Magnetic Nanoparticles of New Broad pH Active Protease from Penicillium Aurantiogriseum. Protein Expr. Purif. 2018, 147, 22–28. DOI: 10.1016/j.pep.2018.01.016.
   PubMed Web of Science ®Google Scholar
• Sumantha, A.; Larroche, C.; Pandey, A. Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective. Food Technol. Biotechnol. 2006, 44, 211–220. ISSN 1330–9862.
   Web of Science ®Google Scholar
• Rao, M. B.; Tanksale, A. M.; Ghatge, M. S.; Deshpande, V. V. Molecular and Biotechnological Aspects of Microbial Proteases. Microbiol. Mol. Biol. Rev. 1998, 62, 597–635. DOI: 10.1128/MMBR.62.3.597-635.1998.
   PubMed Web of Science ®Google Scholar
• de Souza, P. M.; Bittencourt, M. L. d A.; Caprara, C. C.; de Freitas, M.; de Almeida, R. P. C.; Silveira, D.; Fonseca, Y. M.; Ferreira Filho, E. X.; Pessoa Junior, A.; Magalhães, P. O. A Biotechnology Perspective of Fungal Proteases. Braz. J. Microbiol. 2015, 46, 337–346. DOI: 10.1590/S1517-838246220140359.
   PubMed Web of Science ®Google Scholar
• Razzaq, A.; Shamsi, S.; Ali, A.; Ali, Q.; Sajjad, M.; Malik, A.; Ashraf, M. Microbial Proteases Applications. Front. Bioeng. Biotechnol. 2019, 7, 110. DOI: 10.3389/fbioe.2019.00110.
   PubMed Web of Science ®Google Scholar
• Sandhya, C.; Sumantha, A.; Szakacs, G.; Pandey, A. Comparative Evaluation of Neutral Protease Production by Aspergillus oryzae in Submerged and Solid-State Fermentation. Process Biochem. 2005, 40, 2689–2694. DOI: 10.1016/j.procbio.2004.12.001.
   Web of Science ®Google Scholar
• Malathi, S.; Chakraborty, R. Production of Alkaline Protease by a New Aspergillus flavus Isolate under Solid-Substrate Fermentation Conditions for Use as a Depilation Agent. Appl. Environ. Microbiol. 1991, 57, 712–716. DOI: 10.1128/AEM.57.3.712-716.1991.
   PubMed Web of Science ®Google Scholar
• Franco, D. G.; Spalanzani, R. N.; Lima, E. E.; Marchetti, C. R.; Silva, P. O.; Masui, D. C.; Giannesi, G. C.; Zanoelo, F. F. Biochemical Properties of a Serine Protease from Aspergillus flavus and Application in Dehairing. Biocatal. Biotransform. 2017, 35, 249–259. DOI: 10.1080/10242422.2017.1322584.
   Web of Science ®Google Scholar
• Matkawala, F.; Nighojkar, S.; Kumar, A.; Nighojkar, A. Enhanced Production of Alkaline Protease by Neocosmospora sp. N1 Using Custard Apple Seed Powder as Inducer and Its Application for Stain Removal and Dehairing. Biocatal. Agricul. Biotechnol. 2019, 21, 101310. DOI: 10.1016/j.bcab.2019.101310.
   Web of Science ®Google Scholar
• White, T. J.; Bruns, T.; Lee, S.; Taylor, J. W. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In PCR Protocols: A Guide to the Methods and Applications; Innis, M. A., Gelfand, D. H., Sninsky, J. J., White, T. J., Eds.; Academic Press: New York, 1990; pp. 315–322.
   Google Scholar
• Hall, T. A. BioEdit: A User- Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. Nucleic Ácids Symposium Series 1999, 41, 95–98.
   Google Scholar
• Kumar, S.; Stecher, G.; Tamura, K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol. Biol. Evol. 2016, 33, 1870–1874. DOI: 10.1093/molbev/msw054.
   PubMed Web of Science ®Google Scholar
• Saitou, N.; Nei, M. The Neighbor-Joining Method: A New Method for Reconstructing Phylogenetic Trees. Mol. Biol. Evol. 1987, 4, 406–425. DOI: 10.1093/oxfordjournals.molbev.a040454.
   PubMed Web of Science ®Google Scholar
• Khanna, P.; Sundari, S. S.; Kumar, N. J. Production, Isolation and Partial Purification of Xylanases from an Aspergillus sp. World J. Microbiol. Biotechnol. 1995, 11, 242–243. DOI: 10.1007/BF00704661.
   PubMed Web of Science ®Google Scholar
• Sarath, G.; Zeece, M. G.; Penheiter, A. R. In Protease Assay Methods. Proteolytic Enzymes: A Practical Approach; Beynon, R., Bond, J.S. Ed.; Oxford University Press, New York, NY, 2001; pp. 45–76.
   Google Scholar
• Lowry, H.; Rosebrough, N. J.; Farr, A. L.; Randal, R. J. Protein Measurement with the Folin Phenol Reagent. J. Biol. Chem. 1951, 193, 265–275.
   PubMed Web of Science ®Google Scholar
• Laemmli, U. K. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature 1970, 227, 680–685. DOI: 10.1038/227680a0.
   PubMed Web of Science ®Google Scholar
• Davis, B. J. Methods and Application to Human Serum Proteins, Disc. Electroforesis II 2006, 121, 404–427. DOI: 10.1111/j.1749-6632.1964.tb14213.x.
   Google Scholar
• Erlanger, B.; Kokowsky, N.; Cohen, W. The Preparation and Properties of Two New Chromogenic Substrates of Trypsin. Arch. Biochem. Biophys. 1961, 95, 271–278. DOI: 10.1016/0003-9861(61)90145-X.
   PubMed Web of Science ®Google Scholar
• Bundy, H.,F. Chymotrypsin Catalysed Hydrolysis of N-Acetyl and N-Benzoyl L-Tyrosine p-Nitroanilides. Arch. Biochem. Biophys 1963, 102, 416–422. DOI: 10.1016/0003-9861(63)90249-2.
   PubMed Web of Science ®Google Scholar
• Leone, F. A.; Baranauskas, J. A.; Furriel, R. P. M.; Borin, I. A. SigrafW: An Easy-to-Use Program for Fitting Enzyme Kinetic Data. Biochem. Mol. Biol. Educ. 2005, 33, 399–403. DOI: 10.1002/bmb.2005.49403306399.
   PubMed Web of Science ®Google Scholar
• Sorgatto, M.; Guimarães, N. C. A.; Zanoelo, F. F.; Marques, M. R.; Peixoto-Nogueira, S. C.; Giannesi, G. C. Purification and Characterization of an Extracellular Xylanase Produced by the Endophytic Fungus, Aspergillus terreus, Grown in Submerged Fermentation. Afr. J. Biotechnol. 2012, 11, 8076–8084. DOI: 10.5897/AJB11.2686.
   Google Scholar
• Di Cologna, N. M.; Gómez-Mendoza, D. P.; Zanoelo, F. F.; Giannesi, G. C.; Guimarães, N. C. A.; Moreira, L. R. S.; Ferreira-Filho, E. X.; Ricart, C. A. O. Exploring Trichoderma and Aspergillus Secretomes: Proteomics Approaches for the Identification of Enzymes of Biotechnological Interest. Enzyme Microb. Technol. 2018, 109, 1–10. DOI: 10.1016/j.enzmictec.2017.08.007.
   PubMed Web of Science ®Google Scholar
• Niyonzima, F. N.; More, S. S. Purification and Characterization of Detergent-Compatible Protease from Aspergillus terreus gr. 3 Biotech 2015, 5, 61–70. DOI: 10.1007/s13205-014-0200-6.
   PubMedGoogle Scholar
• Biaggio, R. T.; Silva, R. R.; Rosa, N. G.; Leite, R. S.; Arantes, E. C.; Cabral, T. P. Purification and Biochemical Characterization of an Extracellular Serine Peptidase from Aspergillus terreus. Prep. Biochem. Biotechnol 2016, 46, 294–304. 10.1080/10826068.2015.1031387.
   Web of Science ®Google Scholar
• Hajji, M.; Rebai, A.; Gharsallah, N.; Nasri, M. Optimization of Alkaline Protease Production by Aspergillus Clavatus ES1 in Mirabilis Jalapa Tuber Powder Using Statistical Experimental Design. Appl. Microbiol. Biotechnol. 2008, 79, 915–923. DOI: 10.1007/s00253-008-1508-0.
   PubMed Web of Science ®Google Scholar
• Ao, X-l.; Yu, X.; Wu, D-t.; Li, C.; Zhang, T.; Liu, S-l.; Chen, S-j.; He, L.; Zhou, K.; Zou, L-k. Purification and Characterization of Neutral Protease from Aspergillus oryzae Y1 Isolated from Naturally Fermented Broad Beans. AMB Express 2018, 8, 96DOI: 10.1186/s13568-018-0611-6.
   PubMed Web of Science ®Google Scholar
• Chimbekujwo, K. I.; Ja'afaru, M. I.; Adeyemo, O. M. Purification, Characterization and Optimization Conditions of Protease Produced by Aspergillus Brasiliensis Strain BCW2. Sci. African 2020, 8, e00398. DOI: 10.1016/j.sciaf.2020.e00398.
   Google Scholar
• Anitha, T. S.; Palanivelu, P. Purification and Characterization of an Extracellular Keratinolytic Protease from a New Isolate of Aspergillus parasiticus. Protein Expr. Purif. 2013, 88, 214–220. DOI: 10.1016/j.pep.2013.01.007.
   PubMed Web of Science ®Google Scholar
• Wang, S. L.; Chen, Y. H.; Wang, C. L.; Yen, Y. H.; Chern, M. K. Purification and Characterization of a Serine Protease Extracellularly Produced by Aspergillus fumigatus in a Shrimp and Crab Shell Powder Medium. Enz. Microb. Technol. 2005, 36, 660–665. DOI: 10.1016/j.enzmictec.2004.10.006.
   Web of Science ®Google Scholar
• Chakrabarti, S. K.; Matsumura, N.; Ranu, R. S. Purification and Characterization of an Extracellular Alkaline Serine Protease from Aspergillus terreus (IJIRA 6.2). Curr. Microbiol. 2000, 40, 239–244. DOI: 10.1007/s002849910048.
   PubMed Web of Science ®Google Scholar
• Wang, B.; Wu, W.; Liu, X. Purification and Characterization of a Neutral Serine Protease with Nematicidal Activity from Hirsutella Rhossiliensis. Mycopathologia 2007, 163, 169–176. DOI: 10.1007/s11046-007-0100-y.
   PubMed Web of Science ®Google Scholar
• Negi, S.; Banerjee, R. Characterization of Amylase and Protease Produced by Aspergillus Awamori in a Single Bioreactor. Food Res. Int. 2009, 42, 443–448. DOI: 10.1016/j.foodres.2009.01.004.
   Web of Science ®Google Scholar
• Silva, R. R.; Cabral, T. P. F.; Rodrigues, A.; Cabral, H. Production and Partial Characterization of Serine and Metallo Peptidases Secreted by Aspergillus fumigatus Fresenius in Submerged and Solid State Fermentation. Braz. J. Microbiol. 2013, 44, 235–242. DOI: 10.1590/S1517-83822013000100034.
   PubMed Web of Science ®Google Scholar
• Silva, R. R.; Caetano, R. C.; Okamoto, D. N.; Oliveira, L. C. G.; Bertolin, T. C.; Juliano, M. A.; Juliano, J.; Oliveira, A. H. C.; Rosa, J. C.; Cabral, H. The Identification and Biochemical Properties of the Catalytic Specificity of a Serine Peptidase Secreted by Aspergillus fumigatus Fresenius. Protein Pept. Lett. 2014, 21, 663–671. DOI: 10.2174/0929866521666140408114646.
   PubMed Web of Science ®Google Scholar
• Merheb-Dini, C.; Cabral, H.; Leite, R. S.; Zanphorlin, L. M.; Okamoto, D. N.; Rodriguez, G. O.; Juliano, L.; Arantes, E. C.; Gomes, E.; da Silva, R. Biochemical and Functional Characterization of a Metalloprotease from the Thermophilic Fungus Thermoascus Aurantiacus. J. Agric. Food Chem. 2009, 57, 9210–9217. DOI: 10.1021/jf9017977.
   PubMed Web of Science ®Google Scholar
• Siala, R.; Sellami-Kamoun, A.; Hajji, M.; Abid, I.; Gharsallah, N.; Nasri, M. Extracellular Acid Protease from Aspergillus niger I1: Purification and Characterization. Afr. J. Biotechnol. 2009, 8, 4582–4589. 10.5897/AJB09.063.
   Google Scholar
• Zhou, C.; Qin, H.; Chen, X.; Zhang, Y.; Xue, Y.; Ma, Y. A Novel Alkaline Protease from Alkaliphilic Idiomarina sp. C9-1 with Potential Application for Eco-Friendly Enzymatic Dehairing in the Leather Industry. Sci. Rep. 2018, 8, 16467. DOI: 10.1038/s41598-018-34416-5.
   PubMed Web of Science ®Google Scholar
• Manning, M.; Colón, W. Structural Basis of Protein Kinetic Stability: Resistance to Sodium Dodecyl Sulfate Suggests a Central Role for Rigidity and a Bias Toward Beta-Sheet Structure. Biochemistry 2004, 43, 11248–11254. DOI: 10.1021/bi0491898.
   PubMed Web of Science ®Google Scholar
• Fahrney, D. E.; Gold, A. M. Sulfonyl Fluorides as Inhibitors of Esterases. I. Rates of Reaction with Acetylcholinesterase, α-Chymotrypsin, and Trypsin. J. Amer. Chem. Soc. 1963, 85, 9. 10.1021/ja00890a037.
   Web of Science ®Google Scholar
• Powers, J. C.; Asgian, J. L.; Ekici, O. D.; James, K. E. Irreversible Inhibitors of Serine, Cysteine, and Threonine Proteases. Chem. Rev. 2002, 102, 4639–4750. DOI: 10.1021/cr010182v.
   PubMed Web of Science ®Google Scholar
• Kumar, C. G. Purification and Characterization of a Thermostable Alkaline Protease from Alkalophilic Bacillus pumilus. Lett. Appl. Microbiol. 2002, 34, 13–17. DOI: 10.1046/j.1472-765x.2002.01044.x.
   PubMed Web of Science ®Google Scholar
• Sabotič, J.; Kos, J. Microbial and Fungal Protease Inhibitors-Current and Potential Applications. Appl. Microbiol. Biotechnol. 2012, 93, 1351–1375. DOI: 10.1007/s00253-011-3834-x.
   PubMed Web of Science ®Google Scholar
• Mortuza, M. F.; Rahman, M. H.; Rahman, M. H.; Nahar, A.; Islam Khan, M. R.; Hasan, A. K. M. M.; Rahman, M. Isolation, Biochemical and Genetic Characterization of Extracellular Protease Producing Cattle Hide Dehairing Bacterium- a Potential Alternative to Chemical Dehairing. Ecol. Genet. Gen. 2017, 2, 3–12. DOI: 10.1016/j.egg.2016.11.002.
   Google Scholar
• Zanphorlin, L. M.; Cabral, H.; Arantes, E.; Assis, D.; Juliano, L.; Juliano, M. A.; da-Silva, R.; Gomes, E.; Bonilla-Rodriguez, G. O. Purification and Characterization of a New Alkaline Serine Protease from the Thermophilic Fungus Myceliophthora sp. Process Biochem. 2011, 46, 2137–2143. DOI: 10.1016/j.procbio.2011.08.014.
   Web of Science ®Google Scholar
• Silva, R. R.; Oliveira, L. C. G.; Juliano, M. A.; Juliano, L.; Oliveira, A. H. C.; Rosa, J. C.; Cabral, H. Biochemical and Milk-Clotting Properties and Mapping of Catalytic Subsites of an Extracellular Aspartic Peptidase from Basidiomycete Fungus Phanerochaete chrysosporium. Food Chem. 2017, 225, 45–54. DOI: 10.1016/j.foodchem.2017.01.009.
   PubMed Web of Science ®Google Scholar
• Ida, E. L.; Silva, R. R.; Oliveira, T. B.; Souto, T. B.; Leite, J. A.; Rodrigues, A.; Cabral, H. Biochemical Properties and Evaluation of Washing Performance in Commercial Detergent Compatibility of Two Collagenolytic Serine Peptidases Secreted by Aspergillus fischeri and Penicillium citrinum. Prep. Biochem. Biotechnol. 2017, 47, 282–290. DOI: 10.1080/10826068.2016.1224247.
   PubMed Web of Science ®Google Scholar
• Salihi, A.; Asoodeh, A.; Aliabadian, M. Production and Biochemical Characterization of an Alkaline Protease from Aspergillus oryzae CH93. Int. J. Biol. Macromol. 2017, 94, 827–835. DOI: 10.1016/j.ijbiomac.2016.06.023.
   PubMed Web of Science ®Google Scholar
• Yossan, S.; Reungsang, A.; Yasuda, M. Purification and Characterization of Alkaline Protease from Bacillus megaterium Isolated from Thai Fish Sauce Fermentation Process. Sci. Asia 2006, 32, 377–383. DOI: 10.2306/scienceasia1513-1874.2006.32.377.
   Google Scholar
• Devi, M. K.; Banu, A. R.; Gnanaprabhal, G. R.; Pradeep, B. V.; Palaniswamy, M. Purification, Characterization of Alkaline Protease Enzyme from Native Isolate Aspergillus niger and Its Compatibility with Commercial Detergents. IJST. 2008, 1, 1–6. DOI: 10.17485/ijst/2008/v1i7/29599.
   Google Scholar
• Rani, K.; Rana, R.; Datt, S. Review on Latest Overview of Proteases. Int. J. Curr. Life Sci. 2012, 2, 12–18.
   Web of Science ®Google Scholar
• Zhou, L.; Xu, Q.; Zhang, Y.; Zhou, Z.; Guan, W.; Li, Y. Purification, Characterization and in Vitro Anthelmintic Activity of a Neutral Metalloprotease from Laccocephalum Mylittae. Chin. J. Chem. Eng. 2010, 18, 122–128. DOI: 10.1016/S1004-9541(08)60332-8.
   Web of Science ®Google Scholar
• Shankar, S.; Rao, M.; Laxman, R. S. Purification and Characterization of an Alkaline Protease by a New Strain of Beauveria sp. Process Biochem. 2011, 46, 579–585. DOI: 10.1016/j.procbio.2010.10.013.
   Web of Science ®Google Scholar
• Niyonzima, F. N.; More, S. Purification and Properties of Detergent-Compatible Extracellular Alkaline Protease from Scopulariopsis Spp. Prep. Biochem. Biotechnol. 2014, 44, 738–759. DOI: 10.1080/10826068.2013.854254.
   PubMed Web of Science ®Google Scholar
• Zhang, W.; Geng, X.; Chen, W.; Wang, H.; Ng, T. B. Purification and Characterization of a Novel Protease from the Inky Cap Mushroom, Coprinopsis atramentaria (Agaricomycetes). Int. J. Med. Mushrooms. 2018, 20, 349–358. DOI: 10.1615/IntJMedMushrooms.2018025810.
   PubMed Web of Science ®Google Scholar
• Rodríguez, K. L.; Higuera, B. L.; Martínez, S. T. Induction of Proteases Secreted by Fusarium oxysporum f. sp. Dianthi in the Presence of Carnation Root Cell Walls. Biochemical Characterization of a Serine Protease. J. Plant. Pathol. 2017, 99, 609–617. DOI: 10.4454/jpp.v99i3.3984.
   Web of Science ®Google Scholar
• Sivasubramanian, S.; Manohar, B. M.; Puvanakrishnan, R. Mechanism of Enzymatic Dehairing of Skins Using a Bacterial Alkaline Protease. Chemosphere 2008, 70, 1025–e1034. DOI: 10.1016/j.chemosphere.2007.07.084.
   PubMed Web of Science ®Google Scholar
• Zambare, V.; Nilegaonkar, S.; Kanekar, P. A Novel Extracellular Protease from Pseudomonas aeruginosa MCM B-327: Enzyme Production and Its Partial Characterization. N Biotechnol. 2011, 28, 173–181. DOI: 10.1016/j.nbt.2010.10.002.
   PubMed Web of Science ®Google Scholar
• Chellapandi, P. Production and Preliminary Characterization of Alkaline Protease from Aspergillus flavus and Aspergillus terreus. Electron J. Chem. 2010, 7, 479–482. DOI: 10.1155/2010/502583.
   Google Scholar
• Laxman, R. S.; Sonawane, A. P.; More, S. V.; Rao, B. S.; Rele, M. V.; Jogdand, V. V.; Deshpande, V. V.; Rao, M. B. Optimization and Scale up of Production of Alkaline Protease from Conidiobolus Coronatus. Process. Biochem. 2005, 40, 3152–3158. DOI: 10.1016/j.procbio.2005.04.005.
   Web of Science ®Google Scholar
• Abdel-Fattah, A. M. Novel Keratinase from Marine Nocardiopsis dassonvillei NRC2aza Exhibiting Remarkable Hide Dehairing. Egypt. Pharmaceut. J. 2013, 12, 142–147. DOI: 10.4103/1687-4315.124016.
   Google Scholar
• Gillespie, J. M. The Depilation of Sheep Skins with Enzymes. J. Soc. Leath. Trad. Chem. 1953, 37, 344–353.
   Google Scholar
• Dayanandan, A.; Kanagaraj, J.; Sounderraj, L.; Govindaraju, R.; Rajkumar, G. S. Application of an Alkaline Protease in Leather Processing: An Ecofriendly Approach. J. Clean. Prod. 2003, 11, 533–536. DOI: 10.1016/S0959-6526(02)00056-2.
   Web of Science ®Google Scholar
• Puvanakrishnan, R.; Dhar, S. C. Enzyme Technology in Beamhouse Pratice. Enzymes in Dehairing. DICLAI Publication Chennai. 1988; pp. 92–120.
   Google Scholar
• Malathi, S.; Dhar, S. C. Production of Extracellular Proteases by an Aspergillus flavus Isolate and Its Application in the Depilation of Skins. Leather Sci. 1987, 34, 67–76.
   Google Scholar
• Dettmer, A.; Cavalli, É.; Ayub, M. A. Z.; Gutterres, M. Environmentally Friendly Hide Unhairing: enzymatic Hide Processing for the Replacement of Sodium Sulfide and Delimig. J. Clean. Prod. 2013, 47, 11–18. DOI: 10.1016/j.jclepro.2012.04.024.
   Web of Science ®Google Scholar