Expression and characterization of cholesterol oxidase with high thermal and pH stability from Janthinobacterium agaricidamnosum

References

• Smith, A. G.; Brooks, C. J. Application of Cholesterol Oxidase in the Analysis of Steroids. J. Chromatogr. 1974, 101, 373–378.
   PubMed Web of Science ®Google Scholar
• MacLachlan, J.; Wotherspoon, A. T.; Ansell, R. O.; Brooks, C. J. Cholesterol Oxidase: sources, Physical Properties and Analytical Applications. J. Steroid Biochem. Mol. Biol. 2000, 72, 169–195.
   PubMed Web of Science ®Google Scholar
• Doukyu, N. Characteristics and Biotechnological Applications of Microbial Cholesterol Oxidases. Appl. Microbiol. Biotechnol. 2009, 83, 825–837.
   PubMed Web of Science ®Google Scholar
• Doukyu, N.; Aono, R. Two Moles of O2 Consumption and One Mole of H2O2 Formation during Cholesterol Peroxidation with Cholesterol Oxidase from Pseudomonas sp. strain ST-200. Biochem. J. 1999, 341, 621–627.
   PubMed Web of Science ®Google Scholar
• Purcell, J. P.; Greenplate, J. T.; Jennings, M. G.; Ryerse, J. S.; Pershing, J. C.; Sims, S. R.; Prinsen, M. J.; Corbin, D. R.; Tran, M.; Sammons, R. D. Cholesterol Oxidase: A Potent Insecticidal Protein Active against Boll Weevil Larvae. Biochem. Biophys. Res. Commun. 1993, 196, 1406–1413.
   PubMed Web of Science ®Google Scholar
• Dieth, S.; Tritsch, D.; Biellmann, J.-F. Resolution of Allylic Alcohols by Cholesterol Oxidase Isolated from Rhodococcus erythropolis. Tetrahedron Lett. 1995, 36, 2243–2246.
   Web of Science ®Google Scholar
• Mathieu, J. M.; Wang, F.; Segatori, L.; Alvarez, P. J. Increased Resistance to Oxysterol Cytotoxicity in Fibroblasts Transfected with a Lysosomally Targeted Chromobacterium Oxidase. Biotechnol Bioeng. 2012, 109, 2409–2415.
   PubMed Web of Science ®Google Scholar
• Wang, M.; Wang, S.; Zong, G.; Hou, Z.; Liu, F.; Liao, D. J.; Zhu, X. Improvement of Natamycin Production by Cholesterol Oxidase Overexpression in Streptomyces Gilvosporeus. J. Microbiol. Biotechnol. 2016, 26, 241–247.
   PubMed Web of Science ®Google Scholar
• Chen, Y.-R.; Huang, H.-H.; Cheng, Y.-F.; Tang, T.-Y.; Liu, W.-H. Expression of a Cholesterol Oxidase Gene from Arthrobacter simplex in Escherichia coli and Pichia pastoris. Enzyme Microb. Technol. 2006, 39, 854–860.
   Web of Science ®Google Scholar
• Fujishiro, K.; Uchida, H.; Shimokawa, K.; Nakano, M.; Sano, F.; Ohta, T.; Kayahara, N.; Aisaka, K.; Uwajima, T. Purification and Properties of a New Brevibacterium Sterolicum Cholesterol Oxidase Produced by E. coli MM294/pnH10. FEMS Microbiol. Lett. 2002, 215, 243–248.
   PubMed Web of Science ®Google Scholar
• Brzostek, A.; Dziadek, B.; Rumijowska-Galewicz, A.; Pawelczyk, J.; Dziadek, J. Cholesterol Oxidase is Required for Virulence of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2007, 275, 106–112.
   PubMed Web of Science ®Google Scholar
• Doukyu, N.; Ishikawa, M. Cholesterol Oxidase from Rhodococcus erythropolis with High Specificity Toward β-Cholestanol and Pytosterols. PLOS One. 2020, 15, e0241126.
   PubMed Web of Science ®Google Scholar
• Murooka, Y.; Ishizaki, T.; Nimi, O.; Maekawa, N. Cloning and Expression of a Streptomyces Cholesterol Oxidase Gene in Streptomyces lividans with Plasmid pIJ702. Appl. Environ. Microbiol. 1986, 52, 1382–1385.
   PubMed Web of Science ®Google Scholar
• Mahmoud, H. E.; El-Far, S. W.; Embaby, A. M. Cloning, Expression, and in Silico Structural Modeling of Cholesterol Oxidase of Acinetobacter sp. strain RAMD in E. coli. FEBS Open Bio. 2021, 11, 2560–2575.
   PubMed Web of Science ®Google Scholar
• Doukyu, N.; Aono, R. Cloning, Sequence Analysis and Expression of a Gene Encoding an Organic Solvent- and Detergent-Tolerant Cholesterol Oxidase of Burkholderia cepacia Strain ST-200. Appl. Microbiol. Biotechnol. 2001, 57, 146–152.
   PubMed Web of Science ®Google Scholar
• Doukyu, N.; Aono, R. Purification of Extracellular Cholesterol Oxidase with High Activity in the Presence of Organic Solvents from Pseudomonas sp. Strain ST-200. Appl. Environ. Microbiol. 1998, 64, 1929–1932.
   PubMed Web of Science ®Google Scholar
• Doukyu, N.; Shibata, K.; Ogino, H.; Sagermann, M. Cloning, Sequence Analysis, and Expression of a Gene Encoding Chromobacterium sp. DS-1 Cholesterol Oxidase. Appl. Microbiol. Biotechnol. 2009, 82, 479–490.
   PubMed Web of Science ®Google Scholar
• Doukyu, N.; Nihei, S. Cholesterol Oxidase with High Catalytic Activity from Pseudomonas aeruginosa: Screening, Molecular Genetic Analysis, Expression and Characterization. J. Biosci. Bioeng. 2015, 120, 24–30.
   PubMed Web of Science ®Google Scholar
• Reiss, R.; Faccio, G.; Thöny-Meyer, L.; Richter, M. Cloning, Expression and Biochemical Characterization of the Cholesterol Oxidase CgChoA from Chryseobacterium gleum. BMC Biotechnol. 2014, 14, 46.
   PubMed Web of Science ®Google Scholar
• Vrielink, A.; Ghisla, S. Cholesterol Oxidase: Biochemistry and Structural Features. FEBS J. 2009, 276, 6826–6843.
   PubMed Web of Science ®Google Scholar
• Heuts, D. P.; Scrutton, N. S.; McIntire, W. S.; Fraaije, M. W. What's in a Covalent Bond? On the Role and Formation of Covalently Bound Flavin Cofactors. FEBS J. 2009, 276, 3405–3427.
   PubMed Web of Science ®Google Scholar
• Lincoln, S. P.; Fermor, T. R.; Tindall, B. J. Janthinobacterium agaricidamnosum sp. nov., A Soft Rot Pathogen of Agaricus Bisporus. Int. J. Syst. Evol. Microbiol. 1999, 49, 1577–1589.
   Google Scholar
• Navas, J.; González-Zorn, B.; Ladrón, N.; Garrido, P.; Vázquez-Boland, J. A. Identification and Mutagenesis by Allelic Exchange of choE, Encoding a Cholesterol Oxidase from the Intracellular Pathogen Rhodococcus equi. J. Bacteriol. 2001, 183, 4796–4805.
   PubMed Web of Science ®Google Scholar
• Altschul, S. F.; Gish, W.; Miller, W.; Myers, E. W.; Lipman, D. J. Basic Local Alignment Search Tool. J. Mol. Biol. 1990, 215, 403–410.
   PubMed Web of Science ®Google Scholar
• Bradford, M. M. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Anal. Biochem. 1976, 72, 248–254.
   PubMed Web of Science ®Google Scholar
• Piubelli, L.; Pedotti, M.; Molla, G.; Feindler-Boeckh, S.; Ghisla, S.; Pilone, M. S.; Pollegioni, L. On the Oxygen Reactivity of Flavoprotein Oxidases: An Oxygen Access Tunnel and Gate in Brevibacterium Sterolicum Cholesterol Oxidase. J. Biol. Chem. 2008, 283, 24738–24747.
   PubMed Web of Science ®Google Scholar
• Nishiya, Y.; Harada, N.; Teshima, S. I.; Yamashita, M.; Fujii, I.; Hirayama, N.; Murooka, Y. Improvement of Thermal Stability of Streptomyces Cholesterol Oxidase by Random Mutagenesis and a Structural Interpretation. Protein Eng. 1997, 10, 231–235.
   PubMedGoogle Scholar
• Sun, Y.; Yang, H.; Wang, W. Improvement of the Thermostability and Enzymatic Activity of Cholesterol Oxidase by Site-Directed Mutagenesis. Biotechnol. Lett. 2011, 33, 2049–2055.
   PubMed Web of Science ®Google Scholar
• Sagermann, M.; Ohtaki, A.; Newton, K.; Doukyu, N. Structural Characterization of the Organic Solvent-Stable Cholesterol Oxidase from Chromobacterium sp. DS-1. J. Struct. Biol. 2010, 170, 32–40.
   PubMed Web of Science ®Google Scholar