Liquid Chromatographic Validation of a Quantitation Method for Phytoestrogens, Biochanin‐A, Coumestrol, Daidzein, Formononetin, and Genistein, in Lucerne

References

• Wang , C. , Prasain , J. and Barnes , S. 2002 . Review of the methods used in the determination of phytoestrogens . J. Chromatogr. B , 777 : 3 – 28 .
   PubMed Web of Science ®Google Scholar
• Wilkinson , A. P. , Wahala , K. and Williamson , G. 2002 . Identification and quantification of polyphenol phytoestrogens in foods and human fluids . J. Chromatogr. B , I : 93 – 109 .
   Google Scholar
• Adams , N. 1995 . Detection of the effects of phytoestrogens on sheep and cattle . J. Anim. Sci. , 73 : 1509 – 1515 .
   PubMed Web of Science ®Google Scholar
• Ganmaa , D. , Wang , P. Y. , Qin , L. , Hoshi , K. and Sato , A. 2001 . Is milk responsible for male reproductive disorders? . Med. Hypotheses. , 57 ( 4 ) : 510 – 514 .
   PubMed Web of Science ®Google Scholar
• Kuiper , G. , Lemmen , J. , Carlsson , B. , Corton , J. , Safe , S. , Saag , P. , Burg , B. and Gustafsson , J. 1998 . Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor β . Endocrinology , 139 : 4252 – 4263 .
   PubMed Web of Science ®Google Scholar
• Lundh , T.J.O. , Pettersson , H. and Kiessling , K. H. 1988 . Liquid chromatographic determination of the estrogens daidzein, formononetin, coumestrol, and equol in bovine blood plasma and urine . J. Assoc. Off. Anal. Chem. , 71 : 938 – 941 .
   PubMedGoogle Scholar
• McLachlan , J. A. 2001 . Environmental signalling: what embryos and evolution teach us about endocrine disrupting chemicals . Endocrinol. Rev. , 22 ( 3 ) : 319 – 341 .
   PubMed Web of Science ®Google Scholar
• Murkies , A. L. , Wilcox , G. and Davis , S. R. 1998 . Phytoestrogens . J. Clin. Endocrinol. Metab. , 83 : 297 – 303 .
   PubMed Web of Science ®Google Scholar
• Safe , S. , Foster , W. G. , Lamb , J. , Newbold , R. R. and Kraak , G. 2000 . Estrogenicity and endocrine disruption . CAST. , Issue Paper, July, 16
   Google Scholar
• Setchell , K. D.R. 1998 . Phytoestrogens: The biochemistry, physiology, and implications for human health of soy isoflavones . Am. J. Clin. Nutr. , 68 ( suppl ) : 1333 – 1346 .
   Google Scholar
• Benassayag , C. , Perrot‐Applanat , M. and Ferre , F. 2002 . Phytoestrogens as modulators of steroid action in target cells . J. Chromatogr. B , 777 : 233 – 248 .
   PubMed Web of Science ®Google Scholar
• Aussenac , T. , Lacombe , S. and Daydé , J. 1998 . Quantification of isoflavones by capillary zone electrophoresis in soybean seeds: effects of variety and environment . Am. J. Clin. Nutr. , 68 : 1480S – 1485S .
   Web of Science ®Google Scholar
• Tsukamoto , C. , Shimada , S. , Igita , K. , Kudou , S. , Kokubun , M. , Okubo , K. and Kitamura , K. 1995 . Factors affecting isoflavone content in soybean seeds: changes in isoflavones, saponins, and composition of fatty acids at different temperatures during seed development . J. Agric. Food Chem. , 43 : 1184 – 1192 .
   Web of Science ®Google Scholar
• Hoeck , J. , Fehr , W. , Murphy , P. and Welke , G. 2000 . Influence of genotype and environment on isoflavone contents of soybean . Crop Sci. , 40 : 48 – 51 .
   Google Scholar
• Tiller , S. , Parry , A. and Edwards , R. 1994 . Changes in the accumulation of flavonoid and isoflavonoid conjugates associated with plant age and nodulation in alfalfa . (Medicago sativa). Physiol. Plant. , 91 : 27 – 36 .
   Google Scholar
• Livingston , A. , Bickoff , E. , Guggolz , J. and Thompson , C. 1961 . Quantitative determination of coumestrol in fresh and dried alfalfa . J. Agric. Food Chem. , 9 ( 2 ) : 135 – 137 .
   Google Scholar
• Franke , A. , Custer , L. J. , Cerna , C. and Narala , K. 1994 . Quantitation of phytoestrogens in legumes by HPLC . J. Agric. Food Chem. , 42 : 1905 – 1913 .
   Web of Science ®Google Scholar
• Murphy , P. A. , Song , T. , Buseman , G. and Barua , K. 1997 . Isoflavones in soy‐based infant formulas . J. Agric. Food Chem. , 45 : 4635 – 4638 .
   Web of Science ®Google Scholar
• Wang , G. , Kuan , S. , Francis , O. , Ware , G. and Carman , A. 1990 . A simplified HPLC method for the determination of phytoestrogens in soybean and its processed products . J. Agric. Food Chem. , 38 : 185 – 190 .
   Web of Science ®Google Scholar
• Pettersson , H. and Kiessling , K. 1984 . Liquid chromatographic determination of the plant estrogens coumestrol and isoflavones in animal feed . J. Assoc. Off. Anal. Chem. , 67 : 503 – 506 .
   PubMedGoogle Scholar
• Lindsay , S. 1992 . High Performance Liquid Chromatography. Analytical Chemistry by Open University , 2nd Edn. Edited by: Barnes , J. 337 Chichester, , England : J. Wiley & Sons Ltd. .
   Google Scholar
• Liggins , J. , Mulligan , A. , Runswick , S. and Bingham , S. A. 2002 . Daidzein and genistein content of cereals . Eur. J. Clin. Nutr. , 56 : 961 – 966 .
   PubMed Web of Science ®Google Scholar
• Hubert , L. 2001 . Validation of analytical methods: review and strategy . : 22 www.labcompliance.com. Revision 2.01 July
   Google Scholar
• Castro , AF. 2000 . “ Validação de métodos internos de ensaio em análise química ” . Portugal, Fev. GT03 Guia RELACRE 13. Ed
   Google Scholar