Simultaneous Determination of Testosterone and its Major Metabolite Epitestosterone in Biological Fluids by HPLC

References

• Elvers , B. , Rounsaville , J. F. and Schulz , G. 1989 . Ullmann's Encyclopedia of Industrial Chemistry Vol. A13 , 101 – 126 . New York : VCH Publishers .
   Google Scholar
• Bee , H. and Kah , H.‐Y. 2003 . Determination of plasma testosterone using a simple liquid chromatographic method . J. Chromatogr. B , 793 : 421 – 426 .
   PubMed Web of Science ®Google Scholar
• Starka , L. 2003 . Epitestosterone . J. Steroid Biochem. & Molec. Biol. , 87 : 27 – 34 .
   PubMed Web of Science ®Google Scholar
• Navajas , R. , Imaz , C. , Carreras , D. , Garcia , M. , Perez , M. and Cortes , R. 1995 . Determination of epitestosterone and testosterone in urine by HPLC . J. Chromatogr. B , 673 : 159 – 164 .
   PubMed Web of Science ®Google Scholar
• Xing‐Fang , L. , Mingsheng , M. , Cheng , A. , Zheng , J. and Tam , Y.‐K. 2002 . Determination of testosterone and its metabolites using liquid chromatography with elevated column temperature and flow rate gradient . Anal. Chim. Acta. , 457 : 165 – 171 .
   Web of Science ®Google Scholar
• Whalley , P. M. , Bakes , D. , Grime , K. and Weaver , R. J. 2001 . Rapid HPLC for the separation of hydroxylated testosterone metabolites . J. Chromatogr. B , 760 : 281 – 288 .
   PubMed Web of Science ®Google Scholar
• Marcos , J. , Pascual , J. A. , Barbosa , J. and Segura , J. 2000 . Quantification of testosterone and epitestosterone in human urine by capillary liquid chromatography . J. Micro. , 12 : 623 – 629 .
   Google Scholar
• Borst , D. and Bowers , L. 2000 . Direct measurement of urinary testosterone and epitestosterone conjugates using HPLC tandem mass spectrometry . J. Mass Spectr. , 35 : 50 – 61 .
   PubMed Web of Science ®Google Scholar
• Bales , M. R.H. , Dubois , J. G. and Hanocq , M. 1998 . Ethyl acetate extraction procedure and isocratic HPLC assay for testosterone metabolites in cell microsomes . J. Chromatogr. B , 706 : 201 – 207 .
   PubMed Web of Science ®Google Scholar
• Magnuson , M. and Sandstrom , R. 2004 . Quantitive analysis of eight testosterone metabolites using column switching and liquid chromatography tandem mass spectrometry . RCM , 18 : 1089 – 1094 .
   Google Scholar
• Zhang , T. , Zhu , Y. and Gunaratna , C. 2002 . Rapid and quantitative determination of metabolites from multiple cytochrome P450 probe substrates by gradient LC‐electrospray ionization‐ion trap MS . J. Chromatogr. B , 780 : 371 – 379 .
   PubMed Web of Science ®Google Scholar
• Arellano , C. , Philibert , C. , Woodley , J. and Houin , G. 2004 . LC‐MS method to assess cytochrome P450, mediated metabolism of testosterone by rat everted gut sacs . J. Chromatogr. B , 807 : 263 – 270 .
   PubMed Web of Science ®Google Scholar
• Venturelli , E. , Cavalleri , A. and Secreto , G. 1995 . Methods for urinary testosterone analysis . J. Chromatogr. B , 671 : 363 – 380 .
   PubMed Web of Science ®Google Scholar
• De Kock , K. J.S. , Delbeke , F. T. , Van Eenoo , P. , Desmet , N. , Roels , K. and De Backer , P. 2001 . Detection and determination of anabolic steroids in nutritional supplements . J. Pharm. & Biomed. Anal. , 25 : 843 – 852 .
   PubMed Web of Science ®Google Scholar
• Saudan , C. , Entenza , J. M. , Baume , N. , Mangin , P. and Saugy , M. 2006 . Short‐term stability of testosterone and epitestosterone conjugates in urine samples: Quantification by liquid chromatography–linear ion trap mass spectrometry . J. Chromatogr. B , 844 : 168 – 174 .
   PubMed Web of Science ®Google Scholar
• Chiyang , He. , Shehong , L. , Huwei , L. , Kean , L. and Feng , L. 2005 . Extraction of testosterone and epitestosterone in human urine using aqueous two‐phase systems of ionic liquid and salt . J. Chromatogr. A , 1082 : 143 – 149 .
   PubMed Web of Science ®Google Scholar