Development of a Validated HPLC Method for the Simultaneous Determination of Anabolic Steroids in Biological Fluids

REFERENCES

• Elvers , B. ; Rounsaville , J.F. ; Schulz , G. Ullmann's Encyclopedia of Industrial Chemistry ; Vol. A13; VCH Publishers : New York , USA , 1989 ; 101 – 126 .
   Google Scholar
• Samanidou , V.F. ; Karageorgou , E.G. ; Papadoyannis , I.N. Simultaneous determination of testosterone and its major metabolite epitestosterone in biological fluids by HPLC . J. Liquid Chromatogr. & Rel. Technol. 2007 , 30 , 1317 – 1331 .
   Web of Science ®Google Scholar
• http://en.wikipedia.org/wiki/Testosterone, 15/11/2007 .
   Google Scholar
• Hong , B.N. ; Yuen , K.H. Determination of plasma testosterone using a simple liquid chromatographic method . J. Chromatogr. B 2003 , 793 , 421 – 426 .
   PubMed Web of Science ®Google Scholar
• Starcevic , B. ; DiStefano , E. ; Wang , C. ; Catlin , D.H. Liquid chromatography-tandem mass spectrometry assay for human serum testosterone and trideuterated testosterone . J. Chromatogr. B 2003 , 792 , 197 – 204 .
   PubMed Web of Science ®Google Scholar
• http://en.wikipedia.org/wiki/Epitestosterone, 15/11/2007 .
   Google Scholar
• Starka , L. Epitestosterone. J. Ster. Biochem. & Mol. Biol. 2003 , 87 , 27 – 34 .
   PubMed Web of Science ®Google Scholar
• Navajas , R. ; Imaz , C. ; Carreras , D. ; Perez , M. ; Rodriguez , C. ; Rodriguez , A.F. ; Cortes , R. Determination of epitestosterone and testosterone in urine by high-performance liquid chromatography . J. Chromatogr. B 1995 , 673 , 159 – 164 .
   PubMed Web of Science ®Google Scholar
• Borts , D.J. ; Bowers , L.D. Direct measurement of urinary testosterone and epitestosterone conjugates using high-performance liquid chromatography/tandem mass spectrometry . J. Mass. Spectrom. 2000 , 35 , 50 – 61 .
   PubMed Web of Science ®Google Scholar
• Garle , M. ; Ocka , R. ; Palonek , E. ; Bjorkhem , I. Increased urinary testosterone/epitestosterone ratios found in Swedish athletes in connection with a a national program, Evaluation of 28 cases . J. Chromatogr. B 1996 , 687 , 55 – 59 .
   PubMed Web of Science ®Google Scholar
• Marcos , J. ; Pascual , J.A. ; Barbosa , J. Quantification of Testosterone and Epitestosterone in human urine by Capillary Liquid Chromatography . J. Microcol. Sepn. 2000 , 12 , 623 – 629 .
   Google Scholar
• Maitre , A. ; Saudan , C. ; Mangin , P. ; Saugy , M. Urinary Analysis of Four Testosterone Metabolites and Pregnanediol by Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry after Oral Administration of Testosterone . J. Anal. Toxicol. 2004 , 28 .
   PubMed Web of Science ®Google Scholar
• Politi , L. ; Groppi , A. ; Polettini , A. Applications of Liquid Chromatography-Mass Spectrometry in Doping Control . J. Anal. Toxicol. 2005 , 29 .
   PubMed Web of Science ®Google Scholar
• Guan , F. ; Uboh , C.E. ; Soma , L.R. ; Luo , Y. ; Rudy , J. ; Tobin , T. Detection, quantification and confirmation of anabolic steroids in equine plasma by liquid chromatography and tandem mass spectrometry . J. Chromatogr. B 2005 , 829 , 56 – 68 .
   PubMed Web of Science ®Google Scholar
• He , C. ; Li , S. ; Liu , H. ; Li , K. ; Liu , F. Extraction of testosterone and epitestosterone in human urine using aqueous two-phase systems of ionic liquid and salt . J. Chromatogr. A 2005 , 1082 , 143 – 149 .
   PubMed Web of Science ®Google Scholar
• http://en.wikipedia.org/wiki/Nandrolone, 15/11/2007 .
   Google Scholar
• Le Bizec , B. ; Monteau , F. ; Gaudin , I. ; Andre , F. Evidence for the presence of endogenous 19-norandrosterone in human urine. J. Chromatogr. B 1999, 723, 157–172.
   PubMed Web of Science ®Google Scholar
• Dehennin , L. ; Bonnaire , Y. ; Plou , P. Human nutritional supplements in the horse: comparative effects of 19-norandrostenedione and 19-norandrostenediol on the 19-norsteroid profile and consequences for doping control . J. Chromatogr. B 2002 , 766 , 257 – 263 .
   PubMed Web of Science ®Google Scholar
• Tseng , Y.L. ; Kuo , F.H. ; Sun , K.H. Quantification and Profiling of 19-Norandrosterone and 19-Noretiocholanolone in Human Urine after Consumption of a Nutritional Supplement and Norsteroids . J. Anal. Toxicol. 2005 , 29 .
   PubMed Web of Science ®Google Scholar
• Strahm , E. ; Saudan , C. ; Sottas , P.E. ; Mangin , P. ; Saugy , M. Direct detection and quantification of 19-norandrosreone sulphate in human urine by liquid chromatography-linear ion trap mass spectrometry . J. Chromatogr. B 2007 , 852 , 491 – 496 .
   PubMed Web of Science ®Google Scholar
• Sanwald , P. ; Blankson , E.A. ; Dulery , B.D. ; Schoun , J. ; Huebert , N.D. ; Dow , J. Isocratic high-performance liquid chromatographic method for the separation of testosterone metabolites . J. Chromatogr. B 1995 , 672 , 207 – 215 .
   PubMed Web of Science ®Google Scholar
• Baltes , M.R.H. ; Dubois , J.G. ; Hanocq , M. Ethyl acetate extraction procedure and isocratic high-performance liquid chromatographic assay for testosterone metabolites in cell microsomes . J Chromatogr. B 1998 , 706 , 201 – 207 .
   PubMed Web of Science ®Google Scholar
• Whalley , P.M. ; Bakes , D. ; Grime , K. ; Weaver , R.J. Rapid high-performance liquid chromatographic method for the separation of hydroxylated testosterone metabolites . J. Chromatogr. B 2001 , 760 , 281 – 288 .
   PubMed Web of Science ®Google Scholar
• Li , X.F. ; Ma , M. ; Cheng , A. ; Zheng , J. ; Tam , Y.K. Determination of testosterone and its metabolites using liquid chromatography with elevated column temperature and flow-rate gradient . Anal. Chim. Acta 2002 , 457 , 165 – 171 .
   Web of Science ®Google Scholar
• Loi , V. ; Vertzou , M. ; Vryonidou , A. ; Phenekos , C. J. Development and validation of a simple reversed-phase high-performance liquid chromatography method for the determination of testosterone in serum of males . Pharm. Biomed. Anal. 2006 , 41 , 527 – 532 .
   PubMed Web of Science ®Google Scholar
• Shinamoto , A. ; Liu , J. ; Kozawa , S. ; Fujimiya , T. Determination of endogenous testosterone in rat tissues following fetal alcohol exposure using HPLC with UV detection . J. Chromatogr. B 2006 , 836 , 69 – 73 .
   PubMed Web of Science ®Google Scholar
• Draisci , R. ; Palleschi , L. ; Ferretti , L. ; Lucentini , L. ; Cammarata , P. Quantification of anabolic hormones and their metabolites in bovine serum and urine by liquid chromatography-tandem mass spectrometry . J. Chromatogr. A 2000 , 870 , 511 – 522 .
   PubMed Web of Science ®Google Scholar
• Saudan , C. ; Entenza , J.M. ; Baume , N. ; Mangin , P. ; Saugy , M. Short-term stabilityh of testosterone and epitestosterone conjugates in urine samples: Quantification by liquid chromatography-linear ion trap mass spectrometry . J. Chromatogr. B 2006 , 844 , 168 – 174 .
   PubMed Web of Science ®Google Scholar
• De Cock , K.J.S. ; Delbeke , F.T. ; Van Eenoo , P. ; Desmet , N. ; Roels , K. ; De Backer , P. Detection and determination of anabolic steroids in nutritional supplements . J. Pharm. Biomed. Anal. 2001 , 25 , 843 – 852 .
   PubMed Web of Science ®Google Scholar
• Liu , H. ; He , C. ; Wen , D. ; Liu , F. ; Li , K. Extraction of testosterone and epitestosterone in human urine using 2-propanol-salt-H2O system. Anal. Chim. Acta 2006, 557, 329–336.
   Web of Science ®Google Scholar